Project Information
Rapid improvement of domestication traits in an orphan crop by genome editing
Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics resembling wild relatives. We developed genomic resources and efficient transformation in the orphan Solanaceae crop 'groundcherry' (Physalis pruinosa) and used clustered regularly interspaced short palindromic repeats (CRISPR)-CCRISPR-associated protein-9 nuclease (Cas9) (CRISPR-CCas9) to mutate orthologues of tomato domestication and improvement genes that control plant architecture, flower production and fruit size, thereby improving these major productivity traits. Thus, translating knowledge from model crops enables rapid creation of targeted allelic diversity and novel breeding germplasm in distantly related orphan crops.Lab: Lippman Lab, Cold Spring Harbor Laboratory; Van Eck lab, Boyce Thompson Institute
Contact: lippman@cshl.edu; jv27@cornell.edu
List of Transformation
| Transformation | Description | Genetic Background | Construct | Publication |
|---|---|---|---|---|
| AGO7-sgRNA1 | transformation AGO7-sgRNA1 to Physalis pruinosa | Physalis pruinosa | AGO7-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| AGO7-sgRNA2 | transformation AGO7-sgRNA2 to Physalis pruinosa | Physalis pruinosa | AGO7-sgRNA2 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| AGO7-sgRNA3 | transformation AGO7-sgRNA3 to Physalis pruinosa | Physalis pruinosa | AGO7-sgRNA3 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| CLV1-sgRNA1 | transformation CLV1-sgRNA1 to Physalis pruinosa | Physalis pruinosa | CLV1-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| CLV1-sgRNA3 | transformation CLV1-sgRNA3 to Physalis pruinosa | Physalis pruinosa | CLV1-sgRNA3 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| CRTL-B-sgRNA1 | transformation CRTL-B-sgRNA1 to Physalis pruinosa | Physalis pruinosa | CRTL-B-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| CRTL-B-sgRNA2 | transformation CRTL-B-sgRNA2 to Physalis pruinosa | Physalis pruinosa | CRTL-B-sgRNA2 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| J2/EJ2-sgRNA1 | transformation J2/EJ2-sgRNA1 to Physalis pruinosa | Physalis pruinosa | J2/EJ2-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| J2/EJ2-sgRNA2 | transformation J2/EJ2-sgRNA2 to Physalis pruinosa | Physalis pruinosa | J2/EJ2-sgRNA2 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| MPF2-sgRNA1 | transformation MPF2-sgRNA1 to Physalis pruinosa | Physalis pruinosa | MPF2-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| MPF2-sgRNA2 | transformation MPF2-sgRNA2 to Physalis pruinosa | Physalis pruinosa | MPF2-sgRNA2 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| OVATE-sgRNA1 | transformation OVATE-sgRNA1 to Physalis pruinosa | Physalis pruinosa | OVATE-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| OVATE-sgRNA2 | transformation OVATE-sgRNA2 to Physalis pruinosa | Physalis pruinosa | OVATE-sgRNA2 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| SOV1-sgRNA1 | transformation SOV1-sgRNA1 to Physalis pruinosa | Physalis pruinosa | SOV1-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| SOV1-sgRNA2 | transformation SOV1-sgRNA2 to Physalis pruinosa | Physalis pruinosa | SOV1-sgRNA2 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| SP-sgRNA1 | transformation SP-sgRNA1 to Physalis pruinosa | Physalis pruinosa | SP-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| SP-sgRNA2 | transformation SP-sgRNA2 to Physalis pruinosa | Physalis pruinosa | SP-sgRNA2 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| SP5G-sgRNA1 | transformation SP5G-sgRNA1 to Physalis pruinosa | Physalis pruinosa | SP5G-sgRNA1 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
| SP5G-sgRNA2 | transformation SP5G-sgRNA2 to Physalis pruinosa | Physalis pruinosa | SP5G-sgRNA2 | Rapid improvement of domestication traits in an orphan crop by genome editing.Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB. Nat Plants. 2018 Oct;4(10):766-770. |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
