Project Information
Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal of this project is to take advantage of the natural variation present in tomato and its wild relatives along with the extensive genome sequence data available to discover new genes/loci that contribute to plant immunity. The molecular functions of these genes are then being investigated by using CRISPR/Cas9 and other molecular approaches. Our aim is to develop 150 tomato lines with CRISPR/Cas9-generated mutations in a diverse array of immunity-associated genes and to make these available to researchers for assessing the role of these genes in resistance or susceptibility to bacterial, fungal, viral, oomycete, and nematode pathogens.Lab: Martin Lab
Contact: Gregory Martin: gbm7@cornell.edu
List of Transformation
| Transformation | Description | Genetic Background | Construct | Publication |
|---|---|---|---|---|
| CRT0001 | transformation CRT0001 to Moneymaker | Moneymaker | CTG0001-1; CTG0001-1 | |
| CRT0002 | transformation CRT0002 to RG-prf3 | RG-prf3 | CTG0001-1; CTG0001-1 | |
| CRT0003 | transformation CRT0003 to RG-prf3 | RG-prf3 | CTG0003-1; CTG0003-2 | |
| CRT0004 | transformation CRT0004 to TA537 | TA537 | CTG0003-1; CTG0003-2 | |
| CRT0005 | transformation CRT0005 to Moneymaker | Moneymaker | CTG0005-1; CTG0005-2 | |
| CRT0006 | transformation CRT0006 to RG-prf3 | RG-prf3 | CTG0005-1; CTG0005-2 | |
| CRT0007 | transformation CRT0007 to RG-PtoR | RG-PtoR | CTG0007-1; CTG0007-2; CTG0007-3; CTG0007-4 | |
| CRT0008 | transformation CRT0008 to RG-PtoR | RG-PtoR | CTG0008-1; CTG0008-2; CTG0008-3 | |
| CRT0009 | transformation CRT0009 to RG-prf3 | RG-prf3 | CTG0009-1 | |
| CRT0010 | transformation CRT0010 to RG-prf3 | RG-prf3 | CTG0010-1; CTG0010-1 | |
| CRT0011 | transformation CRT0011 to RG-PtoR | RG-PtoR | CTG0011-1 | |
| CRT0012 | transformation CRT0012 to RG-PtoR | RG-PtoR | CTG0012-1; CTG0012-2 | |
| CRT0013 | transformation CRT0013 to Hawaii 7981 | Hawaii 7981 | CTG0012-1; CTG0012-2 | |
| CRT0016 | transformation CRT0016 to RG-PtoR | RG-PtoR | CTG0016-1; CTG0016-2 | |
| CRT0018 | transformation CRT0018 to RG-PtoR | RG-PtoR | CTG0018-1; CTG0018-2 | |
| CRT0019 | transformation CRT0019 to RG-PtoR | RG-PtoR | CTG0019-1; CTG0019-2 | |
| CRT0020 | transformation CRT0020 to RG-PtoR | RG-PtoR | CTG0020-1; CTG0020-2 | |
| CRT0021 | transformation CRT0021 to RG-PtoR | RG-PtoR | CTG0021-1; CTG0021-2 | |
| CRT0022 | transformation CRT0022 to RG-PtoR | RG-PtoR | CTG0022-1; CTG0022-2; CTG0022-3 | |
| CRT0023 | transformation CRT0023 to RG-PtoR | RG-PtoR | CTG0023-1; CTG0023-2; CTG0023-2 | |
| CRT0024 | transformation CRT0024 to RG-PtoR | RG-PtoR | CTG0024-1; CTG0024-2 | |
| CRT0025 | transformation CRT0025 to RG-PtoR | RG-PtoR | CTG0025-1; CTG0025-2 | |
| CRT0026 | transformation CRT0026 to RG-prf3 | RG-prf3 | CTG0009-1; CTG0010-1; CTG0010-1 | |
| CRT0029 | transformation CRT0029 to RG-PtoR | RG-PtoR | CTG0029-1; CTG0029-2 | |
| CRT0030 | transformation CRT0030 to RG-PtoR | RG-PtoR | CTG0030-1; CTG0030-2 | |
| CRT0031 | transformation CRT0031 to RG-PtoR | RG-PtoR | CTG0031-1; CTG0031-2; CTG0031-2; CTG0031-3 | |
| CRT0032 | transformation CRT0032 to RG-PtoR | RG-PtoR | CTG0032-1; CTG0032-2 | |
| CRT0033 | transformation CRT0033 to RG-PtoR | RG-PtoR | CTG0033-1; CTG0033-2 | |
| CRT0034 | transformation CRT0034 to RG-PtoR | RG-PtoR | CTG0034-1; CTG0034-2 | |
| CRT0035 | transformation CRT0035 to RG-PtoR | RG-PtoR | CTG0035-1; CTG0035-2 | |
| CRT0036 | transformation CRT0036 to RG-prf3 | RG-prf3 | CTG0036-1 | |
| CRT0037 | transformation CRT0037 to RG-PtoR | RG-PtoR | CTG0037-1; CTG0037-2 | |
| CRT0038 | transformation CRT0038 to RG-pto11 | RG-pto11 | CTG0037-1; CTG0037-2 | |
| CRT0039 | transformation CRT0039 to RG-PtoR | RG-PtoR | CTG0039-1; CTG0039-2; CTG0039-3; CTG0039-4; CTG0039-5 | |
| CRT0040 | transformation CRT0040 to RG-PtoR | RG-PtoR | CTG0040-1; CTG0040-2 | |
| CRT0041 | transformation CRT0041 to RG-PtoR | RG-PtoR | CTG0041-1; CTG0041-2 | |
| CRT0042 | transformation CRT0042 to RG-PtoR | RG-PtoR | CTG0042-1; CTG0042-2 | |
| CRT0043 | transformation CRT0043 to RG-PtoR | RG-PtoR | CTG0043-1; CTG0043-2 | |
| CRT0050 | transformation CRT0050 to RG-PtoR | RG-PtoR | CTG0050-1; CTG0050-2 | |
| CRT0051 | transformation CRT0051 to RG-PtoR | RG-PtoR | CTG0051-1; CTG0051-2 | |
| CRT0052 | transformation CRT0052 to RG-PtoR | RG-PtoR | CTG0052-1; CTG0052-2 | |
| CRT0053 | transformation CRT0053 to RG-PtoR | RG-PtoR | CTG0053-1; CTG0053-2 | |
| CRT0054 | transformation CRT0054 to RG-PtoR | RG-PtoR | CTG0054-1; CTG0054-2 | |
| CRT0055 | transformation CRT0055 to RG-PtoR | RG-PtoR | CTG0055-1; CTG0055-2 | |
| CRT0056 | transformation CRT0056 to RG-PtoR | RG-PtoR | CTG0056-1; CTG0056-2 | |
| CRT0057 | transformation CRT0057 to RG-PtoR | RG-PtoR | CTG0057-1; CTG0057-2 | |
| CRT0058 | transformation CRT0058 to RG-PtoR | RG-PtoR | CTG0058-1; CTG0058-2 | |
| CRT0059 | transformation CRT0059 to RG-prf3 | RG-prf3 | CTG0059-1; CTG0059-1; CTG0059-1 | |
| CRT0060 | transformation CRT0060 to RG-PtoR | RG-PtoR | CTG0060-1; CTG0060-2 | |
| CRT0061 | transformation CRT0061 to RG-PtoR | RG-PtoR | CTG0061-1; CTG0061-2 | |
| CRT0062 | transformation CRT0062 to RG-PtoR | RG-PtoR | CTG0062-1; CTG0062-2 | |
| CRT0063 | transformation CRT0063 to RG-PtoR | RG-PtoR | CTG0063-1; CTG0063-2 | |
| CRT0064 | transformation CRT0064 to RG-PtoR | RG-PtoR | CTG0064-1; CTG0064-2 | |
| CRT0065 | transformation CRT0065 to RG-PtoR | RG-PtoR | CTG0065-1; CTG0065-2 | |
| CRT0066 | transformation CRT0066 to RG-PtoR | RG-PtoR | CTG0066-1; CTG0066-2 | |
| CRT0067 | transformation CRT0067 to RG-PtoR | RG-PtoR | CTG0067-1; CTG0067-2 | |
| CRT0068 | transformation CRT0068 to RG-PtoR | RG-PtoR | CTG0068-1; CTG0068-2 | |
| CRT0069 | transformation CRT0069 to RG-PtoR | RG-PtoR | CTG0069-1; CTG0069-2 | |
| CRT0070 | transformation CRT0070 to RG-PtoR | RG-PtoR | CTG0070-1; CTG0070-2 | |
| CRT0071 | transformation CRT0071 to RG-PtoR | RG-PtoR | CTG0071-1; CTG0071-2; CTG0071-2; CTG0071-3 | |
| CRT0072 | transformation CRT0072 to RG-PtoR | RG-PtoR | CTG0072-1; CTG0072-2 | |
| CRT0073 | transformation CRT0073 to RG-PtoR | RG-PtoR | CTG0073-1; CTG0073-2 | |
| CRT0074 | transformation CRT0074 to RG-PtoR | RG-PtoR | CTG0074-1; CTG0074-1 | |
| CRT0075 | transformation CRT0075 to RG-PtoR | RG-PtoR | CTG0075-1 | |
| CRT0076 | transformation CRT0076 to RG-PtoR | RG-PtoR | CTG0076-1; CTG0076-2; CTG0076-3 | |
| CRT0077 | transformation CRT0077 to RG-PtoR | RG-PtoR | CTG0077-1 | |
| CRT0078 | transformation CRT0078 to RG-PtoR | RG-PtoR | CTG0078-1 | |
| CRT0079 | transformation CRT0079 to RG-PtoR | RG-PtoR | CTG0079-1; CTG0079-1; CTG0079-2; CTG0079-2 | |
| CRT0080 | transformation CRT0080 to RG-PtoR | RG-PtoR | CTG0080-1; CTG0080-2 | |
| CRT0081 | transformation CRT0081 to RG-PtoR | RG-PtoR | CTG0081-1; CTG0081-2 | |
| CRT0082 | transformation CRT0082 to RG-PtoR | RG-PtoR | CTG0082-1 | |
| CRT0083 | transformation CRT0083 to RG-PtoR | RG-PtoR | CTG0083-1 | |
| CRT0084 | transformation CRT0084 to RG-PtoR | RG-PtoR | CTG0084-1; CTG0084-1; CTG0084-2; CTG0084-2 | |
| CRT0085 | transformation CRT0085 to RG-PtoR | RG-PtoR | CTG0085-1; CTG0085-2 | |
| CRT0086 | transformation CRT0086 to RG-PtoR | RG-PtoR | CTG0086-1; CTG0086-2; CTG0086-3 | |
| CRT0087 | transformation CRT0087 to RG-PtoR | RG-PtoR | CTG0087-3; CTG0087-4 | |
| CRT0088 | transformation CRT0088 to RG-PtoR | RG-PtoR | CTG0088-1 | |
| CRT0089 | transformation CRT0089 to RG-PtoR | RG-PtoR | CTG0089-1 | |
| CRT0090 | transformation CRT0090 to RG-PtoR | RG-PtoR | CTG0090-1; CTG0090-1; CTG0090-2; CTG0090-2 | |
| CRT0091 | transformation CRT0091 to RG-PtoR | RG-PtoR | CTG0091-1 | |
| CRT0092 | transformation CRT0092 to RG-PtoR | RG-PtoR | CTG0092-1 | |
| CRT0093 | transformation CRT0093 to RG-PtoR | RG-PtoR | CTG0093-1; CTG0093-1; CTG0093-2; CTG0093-2 | |
| CRT0094 | transformation CRT0094 to RG-PtoR | RG-PtoR | CTG0094-1; CTG0094-2 | |
| CRT0095 | transformation CRT0095 to RG-PtoR | RG-PtoR | CTG0095-1; CTG0095-2 | |
| CRT0096 | transformation CRT0096 to RG-PtoR | RG-PtoR | CTG0096-1; CTG0096-2 | |
| CRT0097 | transformation CRT0097 to RG-PtoR | RG-PtoR | CTG0097-1; CTG0097-2 | |
| CRT0098 | transformation CRT0098 to RG-PtoR | RG-PtoR | CTG0098-1; CTG0098-2 | |
| CRT0099 | transformation CRT0099 to RG-PtoR | RG-PtoR | CTG0099-1; CTG0099-2 | |
| CRT0100 | transformation CRT0100 to RG-PtoR | RG-PtoR | CTG0100-1; CTG0100-2 | |
| CRT0101 | transformation CRT0101 to RG-PtoR | RG-PtoR | CTG0101-1; CTG0101-2 | |
| CRT0102 | transformation CRT0102 to RG-PtoR | RG-PtoR | CTG0102-1; CTG0102-2 | |
| CRT0103 | transformation CRT0103 to RG-PtoR | RG-PtoR | CTG0103-1; CTG0103-2 | |
| CRT0104 | transformation CRT0104 to RG-PtoR | RG-PtoR | CTG0104-1; CTG0104-2 | |
| CRT0105 | transformation CRT0105 to RG-PtoR | RG-PtoR | CTG0105-1; CTG0105-2 | |
| CRT0106 | transformation CRT0106 to RG-PtoR | RG-PtoR | CTG0106-1; CTG0106-2 | |
| CRT0107 | transformation CRT0107 to RG-PtoR | RG-PtoR | CTG0107-3; CTG0107-4 | |
| CRT0108 | transformation CRT0108 to RG-PtoR | RG-PtoR | CTG0108-1; CTG0108-2 | |
| CRT0109 | transformation CRT0109 to RG-PtoR | RG-PtoR | CTG0109-1; CTG0109-2 | |
| CRT0110 | transformation CRT0110 to RG-PtoR | RG-PtoR | CTG0110-1; CTG0110-2 | |
| CRT0111 | transformation CRT0111 to RG-PtoR | RG-PtoR | CTG0111-1; CTG0111-2 | |
| CRT0112 | transformation CRT0112 to RG-PtoR | RG-PtoR | CTG0112-1; CTG0112-2 | |
| CRT0113 | transformation CRT0113 to RG-PtoR | RG-PtoR | CTG0113-1; CTG0113-2 | |
| CRT0114 | transformation CRT0114 to RG-PtoR | RG-PtoR | CTG0114-1; CTG0114-2 | |
| CRT0115 | transformation CRT0115 to RG-PtoR | RG-PtoR | CTG0115-1; CTG0115-2 | |
| CRT0116 | transformation CRT0116 to RG-PtoR | RG-PtoR | CTG0116-1; CTG0116-2 | |
| CRT0117 | transformation CRT0117 to RG-PtoR | RG-PtoR | CTG0117-1; CTG0117-2 | |
| CRT0118 | transformation CRT0118 to RG-PtoR | RG-PtoR | CTG0118-1; CTG0118-2 | |
| CRT0119 | transformation CRT0119 to RG-PtoR | RG-PtoR | CTG0119-1; CTG0119-2 | |
| CRT0120 | transformation CRT0120 to RG-PtoR | RG-PtoR | CTG0120-1; CTG0120-2 | |
| CRT0121 | transformation CRT0121 to RG-PtoR | RG-PtoR | CTG0121-1; CTG0121-2 | |
| CRT0122 | transformation CRT0122 to RG-PtoR | RG-PtoR | CTG0122-1; CTG0122-2 | |
| CRT0123 | transformation CRT0123 to RG-PtoR | RG-PtoR | CTG0123-1; CTG0123-2 | |
| CRT0124 | transformation CRT0124 to RG-PtoR | RG-PtoR | CTG0124-1; CTG0124-2 | |
| CRT0125 | transformation CRT0125 to RG-PtoR | RG-PtoR | CTG0125-1; CTG0125-2 | |
| CRT0126 | transformation CRT0126 to RG-prf3 | RG-prf3 | CTG0138-3; CTG0138-4 | |
| CRT0127 | transformation CRT0127 to RG-prf3 | RG-prf3 | CTG0138-16; CTG0138-13; CTG0138-14 | |
| CRT0128 | transformation CRT0128 to RG-prf3 | RG-prf3 | CTG0138-11; CTG0138-18; CTG0138-20 | |
| CRT0129 | transformation CRT0129 to LA2109 | LA2109 | CTG0129-1; CTG0129-2 | |
| CRT0130 | transformation CRT0130 to RG-PtoR | RG-PtoR | CTG0130-1; CTG0130-2 | |
| CRT0131 | transformation CRT0131 to RG-PtoR | RG-PtoR | CTG0131-1; CTG0131-2 | |
| CRT0132 | transformation CRT0132 to RG-PtoR | RG-PtoR | CTG0132-1; CTG0132-2; CTG0132-3; CTG0132-4 | |
| CRT0133 | transformation CRT0133 to RG-PtoR | RG-PtoR | CTG0133-1; CTG0133-2 | |
| CRT0134 | transformation CRT0134 to LA4245 | LA4245 | CTG0134-1; CTG0134-2 | |
| CRT0135 | transformation CRT0135 to LA4245 | LA4245 | CTG0135-1; CTG0135-2 | |
| CRT0136 | transformation CRT0136 to LA4245 | LA4245 | CTG0136-1; CTG0136-2 | |
| CRT0137 | transformation CRT0137 to M82 sp+ | M82 sp+ | CTG0137-1; CTG0137-10; CTG0137-11; CTG0137-12; CTG0137-13; CTG0137-14; CTG0137-15; CTG0137-16; CTG0137-17; CTG0137-18; CTG0137-19; CTG0137-2; CTG0137-20; CTG0137-21; CTG0137-22; CTG0137-23; CTG0137-24; CTG0137-25; CTG0137-26; CTG0137-27; CTG0137-28; CTG0137-29; CTG0137-3; CTG0137-30; CTG0137-31; CTG0137-32; CTG0137-33; CTG0137-34; CTG0137-35; CTG0137-36; CTG0137-37; CTG0137-38; CTG0137-39; CTG0137-4; CTG0137-40; CTG0137-41; CTG0137-42; CTG0137-43; CTG0137-44; CTG0137-45; CTG0137-46; CTG0137-47; CTG0137-48; CTG0137-5; CTG0137-6; CTG0137-7; CTG0137-8; CTG0137-9 | Thomas B. Jacobs, Ning Zhang, Dhruv Patel, and Gregory B. Martin (2017). Generation of a collection of mutant tomato lines using pooled CRISPR libraries. Plant Physiology: pp-00489. |
| CRT0138 | transformation CRT0138 to RG-PtoR | RG-PtoR | CTG0138-16; CTG0138-1; CTG0138-10; CTG0138-11; CTG0138-12; CTG0138-13; CTG0138-14; CTG0138-15; CTG0138-17; CTG0138-18; CTG0138-19; CTG0138-2; CTG0138-20; CTG0138-21; CTG0138-22; CTG0138-23; CTG0138-24; CTG0138-25; CTG0138-26; CTG0138-27; CTG0138-28; CTG0138-29; CTG0138-3; CTG0138-30; CTG0138-4; CTG0138-5; CTG0138-6; CTG0138-7; CTG0138-8; CTG0138-9 | ; Thomas B. Jacobs, Ning Zhang, Dhruv Patel, and Gregory B. Martin (2017). Generation of a collection of mutant tomato lines using pooled CRISPR libraries. Plant Physiology: pp-00489. |
| CRT0139 | transformation CRT0139 to RG-PtoR | RG-PtoR | CTG0139-1; CTG0139-2 | |
| CRT0140 | transformation CRT0140 to RG-PtoR | RG-PtoR | CTG0140-1; CTG0140-2 | |
| CRT0141 | transformation CRT0141 to RG-PtoR | RG-PtoR | CTG0141-1; CTG0141-2; CTG0141-3 | |
| CRT0142 | transformation CRT0142 to RG-PtoR | RG-PtoR | CTG0142-1; CTG0142-2 | |
| CRT0143 | transformation CRT0143 to RG-PtoR | RG-PtoR | CTG0143-1; CTG0143-2 | |
| CRT0144 | transformation CRT0144 to RG-PtoR | RG-PtoR | CTG0144-1; CTG0144-2 | |
| CRT0145 | transformation CRT0145 to RG-PtoR | RG-PtoR | CTG0145-1; CTG0145-2 | |
| CRT0146 | transformation CRT0146 to RG-PtoR | RG-PtoR | CTG0146-1; CTG0146-2 | |
| CRT0147 | transformation CRT0147 to RG-PtoR | RG-PtoR | CTG0147-1; CTG0147-1; CTG0147-2; CTG0147-2 | |
| CRT0148 | transformation CRT0148 to RG-PtoR | RG-PtoR | CTG0148-1; CTG0148-2 | |
| CRT0149 | transformation CRT0149 to RG-PtoR | RG-PtoR | CTG0149-1; CTG0149-2 | |
| CRT0150 | transformation CRT0150 to RG-PtoR | RG-PtoR | CTG0150-1; CTG0150-2 | |
| CRT0151 | transformation CRT0151 to RG-PtoR | RG-PtoR | CTG0151-1; CTG0151-2 | |
| CRT0152 | transformation CRT0152 to RG-PtoR | RG-PtoR | CTG0152-1; CTG0152-1; CTG0152-2; CTG0152-3 | |
| CRT0153 | transformation CRT0153 to RG-PtoR | RG-PtoR | CTG0153-1; CTG0153-2 | |
| CRT0154 | transformation CRT0154 to RG-PtoR | RG-PtoR | CTG0154-1; CTG0154-2 | |
| CRT0155 | transformation CRT0155 to RG-PtoR | RG-PtoR | CTG0155-1; CTG0155-2 | |
| CRT0156 | transformation CRT0156 to RG-PtoR | RG-PtoR | CTG0156-1; CTG0156-2 |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
