gRNA information of J2/EJ2-sgRNA1
gRNA
Name: J2/EJ2-sgRNA1
gRNA sequence: CTTATCATCTTCTCTAATCG
Primer used to get the mutation
Organism: groundcherry (no reference genome, using de novo assembly transcriptome )
Position: Ppr-t_50452:133-152
Feature: Ppr-t_50452 Forward primer: TTCTGTTCTTTGTGATGCTGAAGT
Reverse primer: AATTTCTCTGAGTTCGTTGGAGGA
Project & Transformation
Position: Ppr-t_50452:133-152
Feature: Ppr-t_50452 Forward primer: TTCTGTTCTTTGTGATGCTGAAGT
Reverse primer: AATTTCTCTGAGTTCGTTGGAGGA
Project: Rapid improvement of domestication traits in an orphan crop by genome editing
Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics resembling wild relatives. We developed genomic resources and efficient transformation in the orphan So......
Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics resembling wild relatives. We developed genomic resources and efficient transformation in the orphan So......
Lab: Lippman Lab, Cold Spring Harbor Laboratory; Van Eck lab, Boyce Thompson Institute
Contact: lippman@cshl.edu; jv27@cornell.edu
Transformation: J2/EJ2-sgRNA1 -- Construct: J2/EJ2-sgRNA1
This database is supported by NSF (IOS-1546625) and hosted by BTI.
