gRNA information of J2/EJ2-sgRNA2


gRNA
Name: J2/EJ2-sgRNA2
gRNA sequence: ACGTTGCAGCTATGCTACTC
Primer used to get the mutation
Organism: groundcherry (no reference genome, using de novo assembly transcriptome )
Position: Ppr-t_50452:1727-1746
Feature: Ppr-t_50452
Forward primer: TTCTGTTCTTTGTGATGCTGAAGT
Reverse primer: AATTTCTCTGAGTTCGTTGGAGGA
Project & Transformation
Project: Rapid improvement of domestication traits in an orphan crop by genome editing
Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics resembling wild relatives. We developed genomic resources and efficient transformation in the orphan So......
Lab: Lippman Lab, Cold Spring Harbor Laboratory; Van Eck lab, Boyce Thompson Institute
Contact: lippman@cshl.edu; jv27@cornell.edu
Transformation: J2/EJ2-sgRNA2 -- Construct: J2/EJ2-sgRNA2



This database is supported by NSF (IOS-1546625) and hosted by BTI.