Name: Solyc00g174340
(Exon Intron)
Length: 480
TTAGTAAGGACGTTGTCCGATCCAGTTGCCTACAGGATCATAGTTGCAAGAAATGAACCACCATCCGTTGTTGCAACGTGCCCGACCACAACCTAGTCGGACTGAGTTGCGCCAGACTACTTGAGTATAATGTCTACACTTTTTTCCACCAACACATTGGTTGGTAGCGTAGTTATAGCTTGGCCTCTCGGACACCCACA
ATTGCACGGCTGCCCTCCCCGTGAAGTCACCACCACCCTTGGCAAGATTCTCCCCAGCACCAGAATGAATCAAGTTACAATCACCAGCTCTTGAGTTGGC
ATAGTTTTGTGCTCGGGATGCCAAGTTGGCATCCCAAGACATAGGCCCGACTCCGACTTGGGCACGGGCATCGTTATGAACCGCAAGATAGTCTTGGGGT
GAATTTTGGGCCTCACAAGAGTGAAATATGGCTAATACCATGAGACAAGTGAGTAACAATGAGATGTTGAACAACCCCAT
The mutation and phenotype for selected feature: Solyc00g174340
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0019 | RG-PtoR | CTG0019-2 | CGR0019-2 | CRT0019-1 (T0 ) | -6 bp (26.4%); -4 bp (34.2%); -3 bp (32.6%) | |
| CRT0019 | RG-PtoR | CTG0019-2 | CGR0019-2 | CRT0019-2 (T0 ) |
+1 bp/+1 bp
Is tDNA present: Yes
Mutation sequence: AAAGAAAAATGGGGTTGTTCAACATCTCATTGTTACTCACTTGTCTCATGGTATTAGCCATATTTCACTCTTGTGAGGCC CAAAATTCACCCAAAGACTATCTTGCGGTTCATAACGATGCCCGTGCCCAAGTCGGAGTCGGGCCTATGTCTTGGGATGC CAACTTGGCATCCCGAGCACAAAACTATGCCAACTCAAGAGCTGGTGATTGTAACTTGATTCATTCTGGTGCTGGGGAGA ATCTTGCCAAGGGTGGTGGTGACTTCACGGGGAGGGCAGCCGTGCAATTGTGGGTGTCCGAGAGGCCAAGCTATAACTAC GCTACCAACCAATGTGTTGGTGGAAAACAGTGTAGACATTATACTCAAGTAGTCTGGCGCAACTCAGTCCGACTAGGTTG TGGTCGGGCACGTTGCAACAACCGGATGGTGGTTCATTTCTTGCAACTATGATCCTGTAGGCAACTGGATCGGACAACGT CCTTACTAAAATGATGTATACTTATGACATGTTGCTAGTATTAAATAAAATTCTCATATGAGACGTCGAGAAGTTAAAAT TTAAGTTTGACATATGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Low germination (<10%) |
| CRT0019 | RG-PtoR | CTG0019-2 | CGR0019-2 | CRT0019-3 (T0 ) | -5 bp (32%); -4 bp (31.5%); -3 bp (29.5%) | |
| CRT0019 | RG-PtoR | CTG0019-2 | CGR0019-2 | CRT0019-3-7 (T1 ) |
-5 bp/-5 bp
Is tDNA present: Yes
Mutation sequence: GGGGTTGTTCAACATCTCATTGTTACTCACTTGTCTCATGGTATTAGCCATATTTCACTCTTGTGAGGCCCAAAATTCAC CCAAAGACTATCTTGCGGTTCATAACGATGCCCGTGCCCAAGTCGGAGTCGGGCCTATGTCTTGGGATGCCAACTTGGCA TCCCGAGCACAAAACTATGCCAACTCAAGAGCTGGTGATTGTAACTTGATTCATTCTGGTGCTGGGGAGAATCTTGCCAA GGGTGGTGGTGACTTCACGGGGAGGGCAGCCGTGCAATTGTGGGTGTCCGAGAGGCCAAGCTATAACTACGCTACCAACC AATGTGTTGGTGGAAAACAGTGTAGACATTATACTCAAGTAGTCTGGCGCAACTCAGTCCGACTAGGTTGTGGTCGGGCA CGTTGCAGGATGGTGGTTCATTTCTTGCAACTATGATCCTGTAGGCAACTGGATCGGACAACGTCCTTACTAAAATGATG TATACTTATGACATGTTGCTAGTATTAAATAAAATTCTCATATGAGACGTCGAGAAGTTAAAATTTAAGTTTGACATAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
