Name: Solyc05g005700
(Exon Intron)
Length: 4389
TAAGAGGAGAAATGTGAATGTAATAAAACTAGTTGCCAATTGACATCAAATGCAGTTTGTATATAAAAGAAGTTTTGAAAGAAAATCAACATTTATCGTTATCAACATCATGAACTAGAGCAACTTGATATCACTATAATTTAGTTTTATTTGTAAAAATTGCTTTAAGAGGATTATGTTTATAACCATGCTGGATTCTT
CAATGGTGTCACAACTGCCTTTACTTGCAAGTAATTTTTGAGACCGTATTCTCCCTTTTCTCTTCCCTGACCACTCATCTTATAGCCACCAAAAGGAATT
GTAGCATCAAACGTATCAAAGCAATTAATCCATACCGTCCCAACTCTCAAGGCTCGTGCCAACGTGTTTGCAGTGTCGATGTTCTGTGAAAATACTCCAG
CAGCTAGACCATACCGAGAACTATTAGCTCTCCGTACCACTTCATCAAGATCCCTGTAATGAACAAATACATACCATAAATAGAAGTCGATAATACAGCT
CCATGTACTGTTAACATATATATTTGATCGTTCTCACATACTTAAATTTTAAGATGGACTGTACTGGACCAAAAATCTCATCCTGTGCAATCAGCATATC
ATCCTGACAATTGTACAATTTGTTAGTTATGACAATTTATGGACGTTGATACTAAAAAGCACGCCTATTAAAGTGAAAATGTATATACCTTAACATTAGA
GAAGACGGTGGGCCTAATATAGTATCCCTTTTTACCAAATTGCTCACCGCCAGTTTCAAGAGTAGCTCCACTATCAACACCAGATCTAATGTACTTCATG
ATCTTATCGAACTGTTTTGAATCAATCTGTGTGACAGGAAACTCTCAGGTTCGGTATTGTTACCTTATGAGAAGACTTAAAAGGAAGGAGATATGCTATT
TTTTTTCATTATACAGGTTTAATACATTGGTTTCCAGCATTTCAGATAAGAAGAGTATTCGTTAGGAGATTATGAATACCTGAGGACCCTGCTCAGTGCC
TGATTTAAACGGATCACCAACTACTCGTTTCAAGGCGCGTGCCTTTGCCTTCTCCAGAAATTCATCATAGACCTTCTCGTGAACATAGGTCCGAGAACCA
GCACAACAACATTGGCCCTTTAAAATTTATGTCATAGGTGTTACCGTGTTAGTATCTAACATGAACCTGACCAACACCCGACGAAGAAGTATAGATAGTA
AACATTACCTGATTAAAGAAGAGAGCAAAATGAGCTTGTTCAACGGCCGTATCAATATCAGCATCCTCACAAACAATAAAAGGGGATTTCCCTCCAAGTT
CCAATGTCACAGGCTTAAGATTGCTCTTAGCAGCCAATTCAAGTATGGTTTTCCCGGTTTCTGTTGATCCAGTAAAAGCAAGCTGTTGTTGGAACATAAG
TGAATCAGTGTCCAATCCAGTGCAATATACTGGTATCTACTTCCATATGTACAACTATACATAGATATAAATAGATAATTCAAGTCATCTGTTGCCTTAT
CTACTCGTTAGACTTGAATAAGCTACATATGTGCAGACGTGTCTTCCATCCTAATACTTAGAGTTTTGATAAATTGCATATAATAGCACGGCTGGCTAAA
GATTCAAGTGCTACCAACTCTATAATCCCATAAATACAGTCCAGAATTGGTTAAGAATGAGAGTAACGCTAAGCATCAAATACTACCAGAGTTTTCATAC
GGAATGGGATCAACTATTGTAGATGCACTAGATTTCCTGCATAATATGTGTGAAACTTGCCTTCTTTATTGATATATGAAAACTAGTCTTTTCAAGTGAC
AAGATTTCTCCACTGTTGTTTTTGAAATAATACTAATTTTTTGAAATAATACTAATAATATCAATGTGAGGGATATTAGCTCATTTGGCACAACTAGTAT
TTGGAAAAAAATGGCTACAAATGCCAATACATGAATTAAATGCTTGAATATGAACGAGGTTAAGAGTAGTTGTGGTGTCTCTAGCAATCCGTCAAACCAA
ATAAATTGACACAATATGGTGAAATAAAAGCCAAAGAGCCACAAACTACAGATCTTCACACAGAACAGACTAAAAGACGGAATAAAAGTAGATAGAGTTA
CCTTATCCACATCCATATGACTACACAGAGAAGCACCAGCTGTGGCACCATAACCAGAAATTATATTCAGAACACCTTCAGGCAGGCCAGCCTGTTAGTA
ATAACTGAGTATTAGAGATATACCGTATCACTGGAAACATGTAGCAAGTGGCAACAAAAATGTATTAAAAAGAAATAAGTTGTGGCATATTAACAAGTAG
ATGCTTCAGTTTGTGATTATTATTAACACCACAAACTCATGACACTTTAGTGACTTAGTATAAGAAGTAATGGATTATTTTTATAAAATTTGAGAAGTCG
AAACTGATGATTTCACTTTTCATATGATGCCTATAAGATGAGACTTTGTGTACTTGCAGTGTGGAACAACTCTTGTCGGAAATAAAAGAGGAAACTTGAA
CGGTACCTCCTGAAATAGACTTGCTACATACAATGCAGATAATGGGGTCTGCTCAGCCGTCTTTAGCACAATTGTGTTACCACAAGCTAAAGCAGGGCCA
ATCTTCCAAGAAAACATGAGAAGAGGAAAATTCCATGGGATAATCTGACCAGCAACCCCGATCGGTTCATGCAACGTTTGAACATGATATGGTCCATCGG
CAGGAATGGTCATACCATGAATTTTATCTGCCCAGCCTGCAGAATTAAAAGGAGAACATCAGGTGCATAAAACATTTTCAGGAGCCAACCAACATGAAAG
AAGTGGCGAAATACAATTACGCTCTTCATATGGTTATCTAACATACCAGCATAATAACGGAGTAGACGTACAACCATTGGTACTTCAATCTTAGCAGCTT
GTGCATAAGGCTTCCCAGTGTCCCAAGTCTCGAGCGTTGCAATTTGATCGTTATGTTTTTCAATCAGATCAGCAAGGCGCAGCAATATCTTTGACCTTTC
CTGCAGTTATGAACCATTAACGAGTAGATGAAATCTCGCATGCACATTCATTAACATAGCAAAAGAATGCACTAAGAGATACAAAGTAGATACATAAGCA
GTCATCCTAGGCCATGGTCCTTCATCGAAAGCCTTACGAGCAGCAGCAACTGCCCGATTAATATCTTCTGCATCACCTTCAGCAATATGTGCAATTACCT
CCCCTGTCCTGGGGTCGAGAGTCGGGAAAGTTTTTCCTAAAAGTTCAACGAATTAGCTCCATTGTACAACAAAAGGGCCAAAATAGAAGTATAAAGAAGA
GAATTGAGATCCATTATGGTGATTAACATGGATTAACAAATTTACCTGATGCAGAATCAACAAATTGCCCGTTGATAAGAAGTTTAGTATGATCCACATT
GACAGTAGGCTTGACGGGCTCCTCGAAAGCAGCCGCTGTGCTGTATTTATAAGCAGCTATTTTGCCAAGATGGGATCTTTTTCCTACACAAAATAGTTTT
TCTCATTATAGTTCTCTACATTTCATCTCAGAATATTCAACCATTTCAATAAGATTTCTACCTCAAAGATTAAGAATAGTCAAAGTGAAACAGGACTAAA
ACTCTTGAATATTCATCCATATTCAACTTTTGAATAGTTCATAAGCACAAGGTTGTTGAAAAGACATTAAACTCTATACAATATATTCAACCATTTTAAT
GAGTTTTCTGCATTTAAAGACTACAACTCTTCAACAACCTACATCAAAGAAAAATCATGATAATTTAACATGATGGAGATGTACAACAAGACTCATGCCA
CTAAAACACATGTATTTTTTATGATTATGCCGTTCGAGCCAGCTTTTACGCATCTTGACTAATTCCACAGAATACATGTTACCTCCCACCACCAACAAGT
CCTAACGAAAAAAAAACAGAAGTGAGGCTCGGTCAAGTCGTTGAACACCTCTCGAGCTAAGTGTGAACACTATTGATACTCGATCCTCCTAGTGATAGAG
TGGGGAGGAAAGTTTAATTTTACTACACATGTTTCTAAGTTTCTTGTTTTTGATTGTATAAAGATGAGATCTTTAAGCATCATGAAATCAAAACCACTAA
AAACACATATAGGGGCTCCAACATTATTGCTAAAACACACAAAAGATTGAAACTTTTTATCCAAATCTTGAAATTCTATTAACAAGATCATGAAATTCAT
TAATTTAACCCCATAATTAATTCAACAAAAATAATCAAAATCCATTAAACAGAGAAACATAAAAGTAAAAAAAAAAAATACCCTTTGATAGCAGATGAAC
AGAACGAGAGAGAAATACACGAGCCGCCATTATTGGAGATGAAAATTACAGCACTGTTTTCAAGGAGAAAAAAAAGTAGCAAACTTTAT
The mutation and phenotype for selected feature: Solyc05g005700
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0060 | RG-PtoR | CTG0060-1 | CGR0060-1 | CRT0060-2 (T0 ) | -4 bp (19.8%); -5 bp (40.4%); -13 bp (13.9%) | |
| CRT0060 | RG-PtoR | CTG0060-1 | CGR0060-1 | CRT0060-2-1 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: CTTTGAGGTAGAAATCTTATTGAAATGGTTGAATATTCTGAGATGAAATGTAGAGAACTATAATGAGAAAAACTATTTTG TGTAGGAAAAAGATCCCATCTTGGCAAAATAGCTGCTTATAAATACAGCACAGCGGCTGCTTTCGAGGAGCCCGTCAAGC CTACTGTCAATGTGGATCATACTAAACTTCTTATCAACGGGCAATTTGTTGATTCTGCATCAGGTAAATTTGTTAATCCA TGTTAATCACCATAATGGATCTCAATTCTCTTCTTTATACTTCTATTTTGGCCCTTTTGTTGTACAATGGAGCTAATTCG TTGAACTTTTAGGAAAAACTTTCCCGACTCTCGACCCCAGGACAGGGGAGGTAATTGCACATATTGCTGAAGGTGATGCA GAAGATATTAATCGGGCAGTTGCTGCTGCTCGTAAGGCTTTCGATGAAGGACCATGGCCTAGGATGACTGCTTATGTATC TACTTTGTATCTCTTAGTGCATTCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0060 | RG-PtoR | CTG0060-1 | CGR0060-1 | CRT0060-2-20 (T1 ) |
-4 bp and -9 bp
Is tDNA present: No
Mutation sequence: ATCTTTGAGGTAGAAATCTTATTGAAATGGTTGAATATTCTGAGATGAAATGTAGAGAACTATAATGAGAAAAACTATTT TGTGTAGGAAAAAGATCCCATCTTGGCAAAATAGCTGCTTATAAATACAGCACAGCGGCTGCTTTCGAGGAGCCCGTCAA GCCTACTATGTGGATCATACTAAACTTCTTATCAACGGGCAATTTGTTGATTCTGCATCAGGTAAATTTGTTAATCCATG TTAATCACCATAATGGATCTCAATTCTCTTCTTTATACTTCTATTTTGGCCCTTTTGTTGTACAATGGAGCTAATTCGTT GAACTTTTAGGAAAAACTTTCCCGACTCTCGACCCCAGGACAGGGGAGGTAATTGCACATATTGCTGAAGGTGATGCAGA AGATATTAATCGGGCAGTTGCTGCTGCTTTCGATGAAGGACCATGGCCTAGGATGACTGCTTATGTATCTACTTTGTATC TCTTAGTGCATTCTTTTGCTATGTTAATGAATGTGCATGCGAGATTTCATCTACTCGTTAATGGTTCATAACTGCAGGAA AGGTCAAAGATATTGCTGCGCCTTGCTGATCTGATTGAAAAACAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0060 | RG-PtoR | CTG0060-1 | CGR0060-1 | CRT0060-2-23 (T1 ) |
-5 bp/-5 bp
Is tDNA present: No
Mutation sequence: TTTGAGGTAGAAATCTTATTGAAATGGTTGAATATTCTGAGATGAAATGTAGAGAACTATAATGAGAAAAACTATTTTGT GTAGGAAAAAGATCCCATCTTGGCAAAATAGCTGCTTATAAATACAGCACAGCGGCTGCTTTCGAGGAGCCCGTCAAGCC TACATGTGGATCATACTAAACTTCTTATCAACGGGCAATTTGTTGATTCTGCATCAGGTAAATTTGTTAATCCATGTTAA TCACCATAATGGATCTCAATTCTCTTCTTTATACTTCTATTTTGGCCCTTTTGTTGTACAATGGAGCTAATTCGTTGAAC TTTTAGGAAAAACTTTCCCGACTCTCGACCCCAGGACAGGGGAGGTAATTGCACATATTGCTGAAGGTGATGCAGAAGAT ATTAATCGGGCAGTTGCTGCTGCTCGTAAGGCTTTCGATGAAGGACCATGGCCTAGGATGACTGCTTATGTATCTACTTT GTATCTCTTAGTGCATTCTTTTGCTATGTTAATGAATGTGCATGCGAGATTTCATCTACTCGTTAATGGTTCATAACTGC AGGAAAGGTCAAAGATATTGCTGCGCCTTGCTGATCTGATTGAAAAACATAACGAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0060 | RG-PtoR | CTG0060-1 | CGR0060-1 | CRT0060-6 (T0 ) |
-12 bp/-12 bp
Is tDNA present: Yes
Mutation sequence: CTATTCTTAATCTTTGAGGTAGAAATCTTATTGAAATGGTTGAATATTCTGAGATGAAATGTAGAGAACTATAATGAGAA AAACTATTTTGTGTAGGAAAAAGATCCCATCTTGGCAAAATAGCTGCTTATAAATACAGCACAGCGGCTGCTTTCGAGGA GCCCGTCAAGCCTACTCATACTAAACTTCTTATCAACGGGCAATTTGTTGATTCTGCATCAGGTAAATTTGTTAATCCAT GTTAATCACCATAATGGATCTCAATTCTCTTCTTTATACTTCTATTTTGGCCCTTTTGTTGTACAATGGAGCTAATTCGT TGAACTTTTAGGAAAAACTTTCCCGACTCTCGACCCCAGGACAGGGGAGGTAATTGCACATATTGCTGAAGGTGATGCAG AAGATATTAATCGGGCAGTTGCTGCTGCTCGTAAGGCTTTCGATGAAGGACCATGGCCTAGGATGACTGCTTATGTATCT ACTTTGTATCTCTTAGTGCATTCTTTTGCTATGTTAATGAATGTGCATGCGAGATTTCATCTACTCGTTAATGGTTCATA ACTGCAGGAAAGGTCAAAGATATTGCTGCGCCTTGCTGATCTGATTGAAAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Elongated internodes; fruit shape changed; pathenocarpic fruits |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
