Name: Solyc05g013290
(Exon Intron)
Length: 963
TCATTCAGGATCATCTTGAATGGTAGGCTCTTGGAGACAAAGTGCATACTCCAGTTTCCACAGCACATCACCCATTGATGGCCTATTTTTACTAGACGGAGCTAAGCATTTCATCGCTGTTTCTCCAAACATCCTGAGGGACTCTGGTCTTATTTTGGCCGCAATAGCGGGATCTACAATTTGTTCCAACTGTCCCATCT
TCTGCGTCTCATCATCAGAACTCATCATCTCTGAAAGATATAGGGCAGGCCTAGCACAAAGAACTTCGAATAAAACAACACCGAAAGAATAAACATCAGA
TTTTTCTGTCAGCTGTCCCCACAAAGCATATTCAGGGGCAATGTAGCCTATATTTCCTCTCACTACTGTGCTAAGATGGGTTAGATCAAGCTCGGGCATT
GTCTTAGATATTCCAAAATCAGTAATTTTTGGCACAAAATTCTCATCAAGCAATATGTTTGTACACTTGACATCACGATGTATAACTCCGTTAGTATGAA
GGTAGTGAAGACCTCTGGCTGCCCCTATGCATATCTCCAGCCTCTGCTCCCAGCTCATGCTCATAGTGGGTAGATCTGAGCCATACAAATGGCTCTTGAG
GTTCCCATTCTCCATGTAGTCATAAATTAGAATCATCTCATTTCTTTCATCACAGAATCCTATCAATGAAACCAGATGCGGATGGCTGCAAAATGAGAGA
ATCTCAATTTCTGTTTCGAACTCTTCAATACCTTGTGAGGACTCACGTTTATGCTTTTTCAGGGCGACCTTTGTTCCATCACGCAAAACACCCCTGTAAA
CCTTCCCAAATCCACCCTCTCCAATGAAAAACTTGTCATCAAAGTTGTTAGTTGCTTCCTCCAAATCTACAAAAGGAACTCGATAATTTTCAAAAGGAAT
GCCATAACTCAAGTTAGAAGCATCATTTATGGAATTTGTTGCCTTGGAATACTTGCTTCCCAT
The mutation and phenotype for selected feature: Solyc05g013290
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-1 (T0 ) | WT(48%); -1 bp 47% | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-1-4 (T1 ) | -1 bp/-1 bp | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-1-9 (T1 ) | -1 bp/-1 bp | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-2 (T0 ) | WT(48%); -1 bp 47% | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-2-6 (T1 ) | -1 bp | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-3 (T0 ) | -1 bp 47%; -2 bp 49% | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-4 (T0 ) | +1 bp 48%; -1 bp 48% | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-4-10 (T1 ) | -1 bp | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-4-11 (T1 ) | +1 bp | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-5 (T0 ) | -3 bp 46%; -7 bp 49% | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-6 (T0 ) | -1 bp 45%; -5 bp 49% | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-6-8 (T1 ) | -1 bp (43.5%); -5 bp (46%) | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-6-8-6 (T2 ) | -5 bp | |
| CRT0037 | RG-PtoR | CTG0037-1 | CGR0037-1 | CRT0037-6-8-7 (T2 ) | -5 bp | |
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-1 (T0 ) | -9 bp (35.8%); -1 bp (36.6%); WT (20.7%) | |
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-1-10 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: CTGTGAGTTTTCGATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGT ATTCCAAGGCAACAAATTCGATAAATGATGCTTCTAACTTGATTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTG TAGATTTGGAGGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGT GTTTTGCGTGATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGA AATTGAGATTCTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTC TAATTTATGACTACATGGAGAATGGGAACCTCAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-1-12 (T1 ) | -9 bp/-1 bp | |
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-1-2 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: CTGTGAGTTTTCGATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGT ATTCCAAGGCAACAAATTCGATAAATGATGCTTCTAACTTGATTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTG TAGATTTGGAGGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGT GTTTTGCGTGATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGA AATTGAGATTCTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTC TAATTTATGACTACATGGAGAATGGGAACCTCAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : (Phenotype Image ) Immunity response: More susceptible to DC3000:mut5 (lacks E3 ligase of AvrPtoB) |
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-4 (T0 ) | -6 bp (55%); +1 bp (41%) | |
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-4-13 (T1 ) |
+1 bp /+1 bp
Is tDNA present: Yes
Mutation sequence: GATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGTATTCCAAGGCAA CAAATTCGATAAATGATGCTTCTAACTTGAGTTTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTGTAGATTTGGA GGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGTGTTTTGCGTG ATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGAAATTGAGATT CTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTCTAATTTATGA CTACATGGAGAATGGGAACCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : (Phenotype Image ) Immunity response: More susceptible to DC3000:mut5 (lacks E3 ligase of AvrPtoB) |
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-4-13-1 (T2 ) |
+1 bp/+1 bp
Is tDNA present: Yes
Mutation sequence: GATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGTATTCCAAGGCAA CAAATTCGATAAATGATGCTTCTAACTTGAGTTTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTGTAGATTTGGA GGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGTGTTTTGCGTG ATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGAAATTGAGATT CTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTCTAATTTATGA CTACATGGAGAATGGGAACCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Immunity response: More susceptible to DC3000:mut5 (lacks E3 ligase of AvrPtoB) |
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-4-13-6 (T2 ) |
+1 bp/+1 bp
Is tDNA present: Yes
Mutation sequence: GATTTTGCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGTATTCCAAGGCAA CAAATTCGATAAATGATGCTTCTAACTTGAGTTTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTGTAGATTTGGA GGAAGCAACTAACAACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGTGTTTTGCGTG ATGGAACAAAGGTCGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGAAATTGAGATT CTCTCATTTTGCAGCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTCTAATTTATGA CTACATGGAGAATGGGAACCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Immunity response: More susceptible to DC3000:mut5 (lacks E3 ligase of AvrPtoB) |
| CRT0038 | RG-pto11 | CTG0037-1 | CGR0037-1 | CRT0038-4-14 (T1 ) |
-6 bp/-6 bp
Is tDNA present: Yes
Mutation sequence: GCTGTAGAAGTTTGCCCTGTCAAATACTAGTAGATTCCATTATCTATTAATGGGAAGCAAGTATTCCAAGGCAACAAATT CGATAAATGATGCTTCTAATTATGGCGTTCCTTTTGAAAATTATCGAGTTCCTTTTGTAGATTTGGAGGAAGCAACTAAC AACTTTGATGACAATTTTTTCATTGGAGAGGGTGGATTTGGGAAGGTTTACAGGGGTGTTTTGCGTGATGGAACAAAGGT CGCCCTGAAAAAGCATAAACCTGAGTCCTCACAAGGTATTGAAGAGTTCGAAACAGAAATTGAGATTCTCTCATTTTGCA GCCATCCGCATCTGGTTTCATTGATAGGATTCTGTGATGAAAGAAATGAGATGATTCTAATTTATGACTACATGGAGAAT GGGAACCTCAAGAGCCATTTGTATG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
