CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-1 (T0 ) |
heterozygous
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-1-5 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-2 (T0 ) |
heterozygous
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-2-27 (T1 ) |
+1 bp/+1 bp
Is tDNA present: Yes
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
Immunity response: More susceptible to DC3000dd; decrease in ROS response to csp22
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-2-27-1 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-2-27-2 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-2-27-3 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4 (T0 ) |
heterozygous
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-10 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: GGATAACTATGAGCTGCTTCGGTTGTTGTGACGATGATGACATGCATAAACCTGCTGATCATGGACCATTCATGACCAAC
AATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGACTCTTTGAAATAATAAATTCAGCTG
CAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAGCTCAACCTTAACCACTGAATACTGA
TGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCCTATCAGGTCTATAAGTAAATTAGTG
CAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACCTGGTATGGGAGATATGCCTGCTCTG
CAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAGTCAGGTGAAAGTTGTAGTGTTTAGT
ACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCATGATTTTACTCGCTGAGATTCTATT
AATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTTTAGGCTACAATGCAGGGCAGCGTGT
AACAGAAAGTGCGCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGTTCCTTCTATAACTGTTGATGAGTTAA
AAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATGGAAGGGTATACCATGGTGTCCTGAAA
AGTGGGCGGGCTGCAGCCATTAAAAAGTTAGACTCGAGCAAGCAACCTGATCGAGAATTTTCAGCACAGGTCAGGTCGTT
TGGATTGTCCTACTTTGAACTATTCTATATTATACATAAACCTCTTACCTTGCTTGGATATGATGTTACCATTTCAGGTT
TCCATGGTATCAAGACTAAAACATGAAAACGTGGTTGAGTTACTCGGTTATAGTGTGGATGGCGGTCTTCGTGTGCTGGC
TTATGAGTATGCCCCCAATGGATCTCTCCATGACATTCTTCATGGTTAGTTTCTATAAGCCTTTGTGTATCCATTCTATG
CATGTTGACCAATTAATGATTTGGTTGTTCTAATCTATATATACCGGTGAAATTCAGATTTGGTTGTTCTAATTTATATC
CATCTTGGTACGTTGAGTTTGGAAATGATTGCGCAATCCAAATCCAACTCGGTCATGCGCCTTCCCTATAGTGTGCACTT
TAAGTACTTGACTACTGCACCCTCAAGAAGTTGTCAAAGCTCCTCCCCCAATATATCCCCTTTCTTGAAGTACCTCACCA
GGGTCTTTTTTGACATGTGTCATGGAAGTGTCATGTAAAGTTGCTCTAATCAAGAGTAGACTTGCACCAACACTTCCTCA
GATTTTATGCTACTAATTGGACAAACCCTTAAGTATCTCCTTCTTAATTTCAGATTTTATCCTTTTCTTCTCTGTTGTGC
ATATACATTTCTTTCTCTTGTCCTCTTCTCAAATATATATTTCATTCATAAGTTCCATTTGTTACGTACTTGACTGTGCA
TATAGTGAAAAAGTTTTCTACATATTATATTGGTGGTGACTGGACAGACTTCTGAAAATGATAAGTTGTTAAAATGGAAA
ATGTCTACATATAAGTTTTAAGTTCCTCAGATTGAGTAGCTATTAATCCTAATGACAATAGGGGTTTGAACTCATGCTTG
CTTTAACCTAACCTACTTGGATCCAGATTTTGGTATATTGGGTTTCAATGTATAATTCTGTCCAATCTGGTTCAAGACTG
TGTTAAACATTTTGGACGTGTAAAATTGGACAGGAAGTGTGCACTGGGTTAAGATATAGGGTTTACGGGATATCTGAAGG
TAGAATATTATTATTTTTAGTGTGCTTCCATTCCCCTAGTAGCCTTTTGATAGTCCAAACTTGGAAAGACTGCTGTCACT
AAGGTCATTGCGATTGGCCCACCTCATTTATCAGATATCGGAAGGATAATCTATCAAGGAAGTAGGGAGAGGAAGAGAGC
TTGAAGTGGGTTTCTACATCATACTGATGAAGCTTTATACGGAGATGGTTGTTCCTATATATTTTGACCCAATGTAATTG
AATTTGATTGCAGTCGCGTAAGATGCTCTTCTTTTTCTTCAAAATTTAGTCTAACCCACCTTTTTCTCATGTTAATTTCA
GCGATTGGCACTGCTCCGTTAGTAGGTAAAAGAAAAGGATAAAATCCTAGTATCTTGGAAGTCCATCATTATCCTGCCAA
ATCAATTGCATCCTTCCTCTCTCTCTCTCCCTTATATGTAAATTCTAATATATGGGAACAGCTCCAAAAAGTGATGTTTT
GGGGTTGTATTTACCTATCCATTTCCACTATGTTAAGCAAAATAAAACTTCTGCCGTGCAATTGCTGTTTAATGAAAATT
GTTGTCATTTGCTGACAGCATGTTGTATTTCTATTTAACAGAGCAAATTCCGTAACAAGCCTTTGTTTTCTCTTACAATT
TTCCTTATTTGAACTATTTTCTGACTACGAGGGTATCTATCTCTGTACATATGCGTGTGTATACATATTCCTGTTTTATT
TGGAAGTTTTTGTTGTGTTTTCCTTCTTTATGTATGGCTGATCATTCTCATTTTTTTTACTTTTCTGGAACAACTATTGA
TTGTGATGGTTGTCTCAGCCAAGTTAATGTGTCATCTCATACTAATGCTGAAAACTGATCCGACTACCTATTTTTTCAAA
TTGGTTGTAGCAGCCTCAAGCTACCTGGATTATAAATTATTTTTTTGATGACAAAAGAAATATAAAAAAATATATTCCAC
GTAGCCTGGGGCTGCTACAATCAATTTGTAATTCTTGAAATAGTTAGTCAAATTGGGTTTCTCATATTCCATAATCTTGC
TAATCCAATCTGTAAAATGCAAATAACTTTTCAATTTGTAATTACAGGACGAAAAGGTGTGAAAGGTGCACAGCCAGGTC
CAGTATTATCATGGGCCCAACGGGTTAAAATTGCCGTTGGAGCTGCAAAAGGGCTAGAGTACTTGCATGAAAAAGCACAA
CCACATATTATCCATCGTGATATTAAGTCTAGCAATGTCCTACTCTTTGATGATGATGTTGCTAAGATTGCGGATTTTGA
TTTATCAAATCAAGCTCCTGATATGGCAGCACGCCTTCACTCCACACGTGTTCTTGGAACCTTTGGTTATCATGCTCCTG
AGTGAGTTCACTTCTTAATATTTGATACAAATAGACTGTAAACTTGGTTAACTCACTTAAATGAAGCAATTTTAGCTCAG
GGCCCAAAGGTCCTTCATTTTTATGCTGAATGGACTTTGCTCATGTTGCAGATATGCAATGACTGGTCAGCTGAGTTCAA
AGAGTGATGTTTACAGCTTTGGTGTTGTCCTCCTTGAACTACTCACTGGTCGTAAACCTGTTGATCATACATTACCACGT
GGCCAACAGAGTCTTGTGACATGGGTATAACCCTCAAGATACTGACATTACTATAACTGATGCACCAATTTTTTAGTTGA
TTATTTTGATTCTGAATTGACTTTCTGCACCATGTGTAATCAGGCAACACCGAGACTTAGTGAAGATAAAGTGAAGCAAT
GTGTTGACGCTAGACTCGGTACAGATTACCCTCCTAAGGCCATTGCAAAGGTATGTCACTAGAGCAGAAAATTCTTGACT
ACTAAAATCATAATCCTGCATCTTCTTACATCTCTTCTTGATTTTTCATCAATTCCTATTTCTCAAATTTTGTGTTGCTG
AAACAGATGGCTGCTGTTGCTGCTTTGTGTGTGCAATACGAAGCTGATTTTCGGCCAAATATGAGCATTGTGGTAAAAGC
TCTACAACCTCTTTTGCACGCTCGACCTGCACCTAGTGAAACATCAAACTTGTGATTCTCTCCTTTGCCGCAGCCGCTAC
CAAATGGAATGACAGTGATTGAGATTTGTTTTGTTGAGTTGAAAATGATATTATACTAGTAGTTATCTGGGTAAATAGCA
GACATAATGTGCTTTCCCCCCTTCTTTTACTGGTCTCAATTGAATGTGAATAAATTTATACATGTACTAATGAAAGTAGC
CTTTGCCTATTCTTTTAATGGATATTATTCTTGAGGTCTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 TCAAGACCTCAAGAATAATATCCATTAAAAGAATAGGCAAAGGCTACTTT 50
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TCAAGACCTCAAGAATAATATCCATTAAAAGAATAGGCAAAGGCTACTTT 50
Wildtype 51 CATTAGTACATGTATAAATTTATTCACATTCAATTGAGACCAGTAAAAGA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CATTAGTACATGTATAAATTTATTCACATTCAATTGAGACCAGTAAAAGA 100
Wildtype 101 AGGGGGGAAAGCACATTATGTCTGCTATTTACCCAGATAACTACTAGTAT 150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 AGGGGGGAAAGCACATTATGTCTGCTATTTACCCAGATAACTACTAGTAT 150
Wildtype 151 AATATCATTTTCAACTCAACAAAACAAATCTCAATCACTGTCATTCCATT 200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 AATATCATTTTCAACTCAACAAAACAAATCTCAATCACTGTCATTCCATT 200
Wildtype 201 TGGTAGCGGCTGCGGCAAAGGAGAGAATCACAAGTTTGATGTTTCACTAG 250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TGGTAGCGGCTGCGGCAAAGGAGAGAATCACAAGTTTGATGTTTCACTAG 250
Wildtype 251 GTGCAGGTCGAGCGTGCAAAAGAGGTTGTAGAGCTTTTACCACAATGCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GTGCAGGTCGAGCGTGCAAAAGAGGTTGTAGAGCTTTTACCACAATGCTC 300
Wildtype 301 ATATTTGGCCGAAAATCAGCTTCGTATTGCACACACAAAGCAGCAACAGC 350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 ATATTTGGCCGAAAATCAGCTTCGTATTGCACACACAAAGCAGCAACAGC 350
Wildtype 351 AGCCATCTGTTTCAGCAACACAAAATTTGAGAAATAGGAATTGATGAAAA 400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AGCCATCTGTTTCAGCAACACAAAATTTGAGAAATAGGAATTGATGAAAA 400
Wildtype 401 ATCAAGAAGAGATGTAAGAAGATGCAGGATTATGATTTTAGTAGTCAAGA 450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCAAGAAGAGATGTAAGAAGATGCAGGATTATGATTTTAGTAGTCAAGA 450
Wildtype 451 ATTTTCTGCTCTAGTGACATACCTTTGCAATGGCCTTAGGAGGGTAATCT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 ATTTTCTGCTCTAGTGACATACCTTTGCAATGGCCTTAGGAGGGTAATCT 500
Wildtype 501 GTACCGAGTCTAGCGTCAACACATTGCTTCACTTTATCTTCACTAAGTCT 550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 GTACCGAGTCTAGCGTCAACACATTGCTTCACTTTATCTTCACTAAGTCT 550
Wildtype 551 CGGTGTTGCCTGATTACACATGGTGCAGAAAGTCAATTCAGAATCAAAAT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 CGGTGTTGCCTGATTACACATGGTGCAGAAAGTCAATTCAGAATCAAAAT 600
Wildtype 601 AATCAACTAAAAAATTGGTGCATCAGTTATAGTAATGTCAGTATCTTGAG 650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AATCAACTAAAAAATTGGTGCATCAGTTATAGTAATGTCAGTATCTTGAG 650
Wildtype 651 GGTTATACCCATGTCACAAGACTCTGTTGGCCACGTGGTAATGTATGATC 700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GGTTATACCCATGTCACAAGACTCTGTTGGCCACGTGGTAATGTATGATC 700
Wildtype 701 AACAGGTTTACGACCAGTGAGTAGTTCAAGGAGGACAACACCAAAGCTGT 750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 701 AACAGGTTTACGACCAGTGAGTAGTTCAAGGAGGACAACACCAAAGCTGT 750
Wildtype 751 AAACATCACTCTTTGAACTCAGCTGACCAGTCATTGCATATCTGCAACAT 800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 751 AAACATCACTCTTTGAACTCAGCTGACCAGTCATTGCATATCTGCAACAT 800
Wildtype 801 GAGCAAAGTCCATTCAGCATAAAAATGAAGGACCTTTGGGCCCTGAGCTA 850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 801 GAGCAAAGTCCATTCAGCATAAAAATGAAGGACCTTTGGGCCCTGAGCTA 850
Wildtype 851 AAATTGCTTCATTTAAGTGAGTTAACCAAGTTTACAGTCTATTTGTATCA 900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 851 AAATTGCTTCATTTAAGTGAGTTAACCAAGTTTACAGTCTATTTGTATCA 900
Wildtype 901 AATATTAAGAAGTGAACTCACTCAGGAGCATGATAACCAAAGGTTCCAAG 950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 901 AATATTAAGAAGTGAACTCACTCAGGAGCATGATAACCAAAGGTTCCAAG 950
Wildtype 951 AACACGTGTGGAGTGAAGGCGTGCTGCCATATCAGGAGCTTGATTTGATA 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 951 AACACGTGTGGAGTGAAGGCGTGCTGCCATATCAGGAGCTTGATTTGATA 1000
Wildtype 1001 AATCAAAATCCGCAATCTTAGCAACATCATCATCAAAGAGTAGGACATTG 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1001 AATCAAAATCCGCAATCTTAGCAACATCATCATCAAAGAGTAGGACATTG 1050
Wildtype 1051 CTAGACTTAATATCACGATGGATAATATGTGGTTGTGCTTTTTCATGCAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1051 CTAGACTTAATATCACGATGGATAATATGTGGTTGTGCTTTTTCATGCAA 1100
Wildtype 1101 GTACTCTAGCCCTTTTGCAGCTCCAACGGCAATTTTAACCCGTTGGGCCC 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1101 GTACTCTAGCCCTTTTGCAGCTCCAACGGCAATTTTAACCCGTTGGGCCC 1150
Wildtype 1151 ATGATAATACTGGACCTGGCTGTGCACCTTTCACACCTTTTCGTCCTGTA 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1151 ATGATAATACTGGACCTGGCTGTGCACCTTTCACACCTTTTCGTCCTGTA 1200
Wildtype 1201 ATTACAAATTGAAAAGTTATTTGCATTTTACAGATTGGATTAGCAAGATT 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1201 ATTACAAATTGAAAAGTTATTTGCATTTTACAGATTGGATTAGCAAGATT 1250
Wildtype 1251 ATGGAATATGAGAAACCCAATTTGACTAACTATTTCAAGAATTACAAATT 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1251 ATGGAATATGAGAAACCCAATTTGACTAACTATTTCAAGAATTACAAATT 1300
Wildtype 1301 GATTGTAGCAGCCCCAGGCTACGTGGAATATATTTTTTTATATTTCTTTT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1301 GATTGTAGCAGCCCCAGGCTACGTGGAATATATTTTTTTATATTTCTTTT 1350
Wildtype 1351 GTCATCAAAAAAATAATTTATAATCCAGGTAGCTTGAGGCTGCTACAACC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1351 GTCATCAAAAAAATAATTTATAATCCAGGTAGCTTGAGGCTGCTACAACC 1400
Wildtype 1401 AATTTGAAAAAATAGGTAGTCGGATCAGTTTTCAGCATTAGTATGAGATG 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1401 AATTTGAAAAAATAGGTAGTCGGATCAGTTTTCAGCATTAGTATGAGATG 1450
Wildtype 1451 ACACATTAACTTGGCTGAGACAACCATCACAATCAATAGTTGTTCCAGAA 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1451 ACACATTAACTTGGCTGAGACAACCATCACAATCAATAGTTGTTCCAGAA 1500
Wildtype 1501 AAGTAAAAAAAATGAGAATGATCAGCCATACATAAAGAAGGAAAACACAA 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1501 AAGTAAAAAAAATGAGAATGATCAGCCATACATAAAGAAGGAAAACACAA 1550
Wildtype 1551 CAAAAACTTCCAAATAAAACAGGAATATGTATACACACGCATATGTACAG 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1551 CAAAAACTTCCAAATAAAACAGGAATATGTATACACACGCATATGTACAG 1600
Wildtype 1601 AGATAGATACCCTCGTAGTCAGAAAATAGTTCAAATAAGGAAAATTGTAA 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1601 AGATAGATACCCTCGTAGTCAGAAAATAGTTCAAATAAGGAAAATTGTAA 1650
Wildtype 1651 GAGAAAACAAAGGCTTGTTACGGAATTTGCTCTGTTAAATAGAAATACAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1651 GAGAAAACAAAGGCTTGTTACGGAATTTGCTCTGTTAAATAGAAATACAA 1700
Wildtype 1701 CATGCTGTCAGCAAATGACAACAATTTTCATTAAACAGCAATTGCACGGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1701 CATGCTGTCAGCAAATGACAACAATTTTCATTAAACAGCAATTGCACGGC 1750
Wildtype 1751 AGAAGTTTTATTTTGCTTAACATAGTGGAAATGGATAGGTAAATACAACC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1751 AGAAGTTTTATTTTGCTTAACATAGTGGAAATGGATAGGTAAATACAACC 1800
Wildtype 1801 CCAAAACATCACTTTTTGGAGCTGTTCCCATATATTAGAATTTACATATA 1850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1801 CCAAAACATCACTTTTTGGAGCTGTTCCCATATATTAGAATTTACATATA 1850
Wildtype 1851 AGGGAGAGAGAGAGAGGAAGGATGCAATTGATTTGGCAGGATAATGATGG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1851 AGGGAGAGAGAGAGAGGAAGGATGCAATTGATTTGGCAGGATAATGATGG 1900
Wildtype 1901 ACTTCCAAGATACTAGGATTTTATCCTTTTCTTTTACCTACTAACGGAGC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1901 ACTTCCAAGATACTAGGATTTTATCCTTTTCTTTTACCTACTAACGGAGC 1950
Wildtype 1951 AGTGCCAATCGCTGAAATTAACATGAGAAAAAGGTGGGTTAGACTAAATT 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1951 AGTGCCAATCGCTGAAATTAACATGAGAAAAAGGTGGGTTAGACTAAATT 2000
Wildtype 2001 TTGAAGAAAAAGAAGAGCATCTTACGCGACTGCAATCAAATTCAATTACA 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2001 TTGAAGAAAAAGAAGAGCATCTTACGCGACTGCAATCAAATTCAATTACA 2050
Wildtype 2051 TTGGGTCAAAATATATAGGAACAACCATCTCCGTATAAAGCTTCATCAGT 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2051 TTGGGTCAAAATATATAGGAACAACCATCTCCGTATAAAGCTTCATCAGT 2100
Wildtype 2101 ATGATGTAGAAACCCACTTCAAGCTCTCTTCCTCTCCCTACTTCCTTGAT 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2101 ATGATGTAGAAACCCACTTCAAGCTCTCTTCCTCTCCCTACTTCCTTGAT 2150
Wildtype 2151 AGATTATCCTTCCGATATCTGATAAATGAGGTGGGCCAATCGCAATGACC 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2151 AGATTATCCTTCCGATATCTGATAAATGAGGTGGGCCAATCGCAATGACC 2200
Wildtype 2201 TTAGTGACAGCAGTCTTTCCAAGTTTGGACTATCAAAAGGCTACTAGGGG 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2201 TTAGTGACAGCAGTCTTTCCAAGTTTGGACTATCAAAAGGCTACTAGGGG 2250
Wildtype 2251 AATGGAAGCACACTAAAAATAATAATATTCTACCTTCAGATATCCCGTAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2251 AATGGAAGCACACTAAAAATAATAATATTCTACCTTCAGATATCCCGTAA 2300
Wildtype 2301 ACCCTATATCTTAACCCAGTGCACACTTCCTGTCCAATTTTACACGTCCA 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2301 ACCCTATATCTTAACCCAGTGCACACTTCCTGTCCAATTTTACACGTCCA 2350
Wildtype 2351 AAATGTTTAACACAGTCTTGAACCAGATTGGACAGAATTATACATTGAAA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2351 AAATGTTTAACACAGTCTTGAACCAGATTGGACAGAATTATACATTGAAA 2400
Wildtype 2401 CCCAATATACCAAAATCTGGATCCAAGTAGGTTAGGTTAAAGCAAGCATG 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2401 CCCAATATACCAAAATCTGGATCCAAGTAGGTTAGGTTAAAGCAAGCATG 2450
Wildtype 2451 AGTTCAAACCCCTATTGTCATTAGGATTAATAGCTACTCAATCTGAGGAA 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2451 AGTTCAAACCCCTATTGTCATTAGGATTAATAGCTACTCAATCTGAGGAA 2500
Wildtype 2501 CTTAAAACTTATATGTAGACATTTTCCATTTTAACAACTTATCATTTTCA 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2501 CTTAAAACTTATATGTAGACATTTTCCATTTTAACAACTTATCATTTTCA 2550
Wildtype 2551 GAAGTCTGTCCAGTCACCACCAATATAATATGTAGAAAACTTTTTCACTA 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2551 GAAGTCTGTCCAGTCACCACCAATATAATATGTAGAAAACTTTTTCACTA 2600
Wildtype 2601 TATGCACAGTCAAGTACGTAACAAATGGAACTTATGAATGAAATATATAT 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2601 TATGCACAGTCAAGTACGTAACAAATGGAACTTATGAATGAAATATATAT 2650
Wildtype 2651 TTGAGAAGAGGACAAGAGAAAGAAATGTATATGCACAACAGAGAAGAAAA 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2651 TTGAGAAGAGGACAAGAGAAAGAAATGTATATGCACAACAGAGAAGAAAA 2700
Wildtype 2701 GGATAAAATCTGAAATTAAGAAGGAGATACTTAAGGGTTTGTCCAATTAG 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2701 GGATAAAATCTGAAATTAAGAAGGAGATACTTAAGGGTTTGTCCAATTAG 2750
Wildtype 2751 TAGCATAAAATCTGAGGAAGTGTTGGTGCAAGTCTACTCTTGATTAGAGC 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2751 TAGCATAAAATCTGAGGAAGTGTTGGTGCAAGTCTACTCTTGATTAGAGC 2800
Wildtype 2801 AACTTTACATGACACTTCCATGACACATGTCAAAAAAGACCCTGGTGAGG 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2801 AACTTTACATGACACTTCCATGACACATGTCAAAAAAGACCCTGGTGAGG 2850
Wildtype 2851 TACTTCAAGAAAGGGGATATATTGGGGGAGGAGCTTTGACAACTTCTTGA 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2851 TACTTCAAGAAAGGGGATATATTGGGGGAGGAGCTTTGACAACTTCTTGA 2900
Wildtype 2901 GGGTGCAGTAGTCAAGTACTTAAAGTGCACACTATAGGGAAGGCGCATGA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2901 GGGTGCAGTAGTCAAGTACTTAAAGTGCACACTATAGGGAAGGCGCATGA 2950
Wildtype 2951 CCGAGTTGGATTTGGATTGCGCAATCATTTCCAAACTCAACGTACCAAGA 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2951 CCGAGTTGGATTTGGATTGCGCAATCATTTCCAAACTCAACGTACCAAGA 3000
Wildtype 3001 TGGATATAAATTAGAACAACCAAATCTGAATTTCACCGGTATATATAGAT 3050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3001 TGGATATAAATTAGAACAACCAAATCTGAATTTCACCGGTATATATAGAT 3050
Wildtype 3051 TAGAACAACCAAATCATTAATTGGTCAACATGCATAGAATGGATACACAA 3100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3051 TAGAACAACCAAATCATTAATTGGTCAACATGCATAGAATGGATACACAA 3100
Wildtype 3101 AGGCTTATAGAAACTAACCATGAAGAATGTCATGGAGAGATCCATTGGGG 3150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3101 AGGCTTATAGAAACTAACCATGAAGAATGTCATGGAGAGATCCATTGGGG 3150
Wildtype 3151 GCATACTCATAAGCCAGCACACGAAGACCGCCATCCACACTATAACCGAG 3200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3151 GCATACTCATAAGCCAGCACACGAAGACCGCCATCCACACTATAACCGAG 3200
Wildtype 3201 TAACTCAACCACGTTTTCATGTTTTAGTCTTGATACCATGGAAACCTGAA 3250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3201 TAACTCAACCACGTTTTCATGTTTTAGTCTTGATACCATGGAAACCTGAA 3250
Wildtype 3251 ATGGTAACATCATATCCAAGCAAGGTAAGAGGTTTATGTATAATATAGAA 3300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3251 ATGGTAACATCATATCCAAGCAAGGTAAGAGGTTTATGTATAATATAGAA 3300
Wildtype 3301 TAGTTCAAAGTAGGACAATCCAAACGACCTGACCTGTGCTGAAAATTCTC 3350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3301 TAGTTCAAAGTAGGACAATCCAAACGACCTGACCTGTGCTGAAAATTCTC 3350
Wildtype 3351 GATCAGGTTGCTTGCTCGAGTCTAACTTTTTAATGGCTGCAGCCCGCCCA 3400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3351 GATCAGGTTGCTTGCTCGAGTCTAACTTTTTAATGGCTGCAGCCCGCCCA 3400
Wildtype 3401 CTTTTCAGGACACCATGGTATACCCTTCCATACGATCCCTCACCTATCAA 3450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3401 CTTTTCAGGACACCATGGTATACCCTTCCATACGATCCCTCACCTATCAA 3450
Wildtype 3451 GGCTTTTGTACCAAAGTTGTCTGTGATGTCTTTTAACTCATCAACAGTTA 3500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3451 GGCTTTTGTACCAAAGTTGTCTGTGATGTCTTTTAACTCATCAACAGTTA 3500
Wildtype 3501 TAGAAGGAACAGCAATGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGC 3550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3501 TAGAAGGAACAGCAATGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGC 3550
gRNA TTGC
Wildtype 3551 GCACTTTCTGTTACACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCC 3600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3551 GCACTTTCTGTTACACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCC 3600
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3601 AGTTAGTGAAAACCATAAATCGTACAGGTAAAAAAAAGAATTAATAGAAT 3650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3601 AGTTAGTGAAAACCATAAATCGTACAGGTAAAAAAAAGAATTAATAGAAT 3650
Wildtype 3651 CTCAGCGAGTAAAATCATGACACTATCTAAAGAAAGCATATATTTAGGAT 3700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3651 CTCAGCGAGTAAAATCATGACACTATCTAAAGAAAGCATATATTTAGGAT 3700
Wildtype 3701 AGGCGTAAAGCCAAAGCAATGTACTAAACACTACAACTTTCACCTGACTG 3750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3701 AGGCGTAAAGCCAAAGCAATGTACTAAACACTACAACTTTCACCTGACTG 3750
Wildtype 3751 TTTCAAAATTTACCAAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTT 3800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3751 TTTCAAAATTTACCAAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTT 3800
Wildtype 3801 TGCAGAGCAGGCATATCTCCCATACCAGGTCACAACGACACTAGATTCAA 3850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3801 TGCAGAGCAGGCATATCTCCCATACCAGGTCACAACGACACTAGATTCAA 3850
Wildtype 3851 ACATCATGATCTCTTAACTTCCAAGATACTTGCACTAATTTACTTATAGA 3900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3851 ACATCATGATCTCTTAACTTCCAAGATACTTGCACTAATTTACTTATAGA 3900
Wildtype 3901 CCTGATAGGAAGATTTGCCTATTAGTTCCCAACTCTACTGAGTACTGATA 3950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3901 CCTGATAGGAAGATTTGCCTATTAGTTCCCAACTCTACTGAGTACTGATA 3950
Wildtype 3951 ATGTATTATACATCAGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGA 4000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3951 ATGTATTATACATCAGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGA 4000
Wildtype 4001 GATACTAGGATTGAAACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAA 4050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4001 GATACTAGGATTGAAACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAA 4050
Wildtype 4051 TTTATTATTTCAAAGAGTCTGAAAATATTATTAATAGCAAATAGTACAAG 4100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4051 TTTATTATTTCAAAGAGTCTGAAAATATTATTAATAGCAAATAGTACAAG 4100
Wildtype 4101 GAGGAAATTAACCTGCTGCATTGTTGGTCATGAATGGTCCATGATCAGCA 4150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4101 GAGGAAATTAACCTGCTGCATTGTTGGTCATGAATGGTCCATGATCAGCA 4150
Wildtype 4151 GGTTTATGCATGTCATCATCGTCACAACAACCGAAGCAGCTCATAGTTAT 4200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4151 GGTTTATGCATGTCATCATCGTCACAACAACCGAAGCAGCTCATAGTTAT 4200
Wildtype 4201 CC 4202
||
MutationRev 4201 CC 4202
|
Immunity response: "No significant change in resistance to DC3000dd, ROS not tested"
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-13 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: GGATAACTATGAGCTGCTTCGGTTGTTGTGACGATGATGACATGCATAAACCTGCTGATCATGGACCATTCATGACCAAC
AATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGACTCTTTGAAATAATAAATTCAGCTG
CAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAGCTCAACCTTAACCACTGAATACTGA
TGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCCTATCAGGTCTATAAGTAAATTAGTG
CAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACCTGGTATGGGAGATATGCCTGCTCTG
CAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAGTCAGGTGAAAGTTGTAGTGTTTAGT
ACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCATGATTTTACTCGCTGAGATTCTATT
AATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTTTAGGCTACAATGCAGGGCAGCGTGT
AACAGAAAGTGCGCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGTTCCTTCTATAACTGTTGATGAGTTAA
AAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATGGAAGGGTATACCATGGTGTCCTGAAA
AGTGGGCGGGCTGCAGCCATTAAAAAGTTAGACTCGAGCAAGCAACCTGATCGAGAATTTTCAGCACAGGTCAGGTCGTT
TGGATTGTCCTACTTTGAACTATTCTATATTATACATAAACCTCTTACCTTGCTTGGATATGATGTTACCATTTCAGGTT
TCCATGGTATCAAGACTAAAACATGAAAACGTGGTTGAGTTACTCGGTTATAGTGTGGATGGCGGTCTTCGTGTGCTGGC
TTATGAGTATGCCCCCAATGGATCTCTCCATGACATTCTTCATGGTTAGTTTCTATAAGCCTTTGTGTATCCATTCTATG
CATGTTGACCAATTAATGATTTGGTTGTTCTAATCTATATATACCGGTGAAATTCAGATTTGGTTGTTCTAATTTATATC
CATCTTGGTACGTTGAGTTTGGAAATGATTGCGCAATCCAAATCCAACTCGGTCATGCGCCTTCCCTATAGTGTGCACTT
TAAGTACTTGACTACTGCACCCTCAAGAAGTTGTCAAAGCTCCTCCCCCAATATATCCCCTTTCTTGAAGTACCTCACCA
GGGTCTTTTTTGACATGTGTCATGGAAGTGTCATGTAAAGTTGCTCTAATCAAGAGTAGACTTGCACCAACACTTCCTCA
GATTTTATGCTACTAATTGGACAAACCCTTAAGTATCTCCTTCTTAATTTCAGATTTTATCCTTTTCTTCTCTGTTGTGC
ATATACATTTCTTTCTCTTGTCCTCTTCTCAAATATATATTTCATTCATAAGTTCCATTTGTTACGTACTTGACTGTGCA
TATAGTGAAAAAGTTTTCTACATATTATATTGGTGGTGACTGGACAGACTTCTGAAAATGATAAGTTGTTAAAATGGAAA
ATGTCTACATATAAGTTTTAAGTTCCTCAGATTGAGTAGCTATTAATCCTAATGACAATAGGGGTTTGAACTCATGCTTG
CTTTAACCTAACCTACTTGGATCCAGATTTTGGTATATTGGGTTTCAATGTATAATTCTGTCCAATCTGGTTCAAGACTG
TGTTAAACATTTTGGACGTGTAAAATTGGACAGGAAGTGTGCACTGGGTTAAGATATAGGGTTTACGGGATATCTGAAGG
TAGAATATTATTATTTTTAGTGTGCTTCCATTCCCCTAGTAGCCTTTTGATAGTCCAAACTTGGAAAGACTGCTGTCACT
AAGGTCATTGCGATTGGCCCACCTCATTTATCAGATATCGGAAGGATAATCTATCAAGGAAGTAGGGAGAGGAAGAGAGC
TTGAAGTGGGTTTCTACATCATACTGATGAAGCTTTATACGGAGATGGTTGTTCCTATATATTTTGACCCAATGTAATTG
AATTTGATTGCAGTCGCGTAAGATGCTCTTCTTTTTCTTCAAAATTTAGTCTAACCCACCTTTTTCTCATGTTAATTTCA
GCGATTGGCACTGCTCCGTTAGTAGGTAAAAGAAAAGGATAAAATCCTAGTATCTTGGAAGTCCATCATTATCCTGCCAA
ATCAATTGCATCCTTCCTCTCTCTCTCTCCCTTATATGTAAATTCTAATATATGGGAACAGCTCCAAAAAGTGATGTTTT
GGGGTTGTATTTACCTATCCATTTCCACTATGTTAAGCAAAATAAAACTTCTGCCGTGCAATTGCTGTTTAATGAAAATT
GTTGTCATTTGCTGACAGCATGTTGTATTTCTATTTAACAGAGCAAATTCCGTAACAAGCCTTTGTTTTCTCTTACAATT
TTCCTTATTTGAACTATTTTCTGACTACGAGGGTATCTATCTCTGTACATATGCGTGTGTATACATATTCCTGTTTTATT
TGGAAGTTTTTGTTGTGTTTTCCTTCTTTATGTATGGCTGATCATTCTCATTTTTTTTACTTTTCTGGAACAACTATTGA
TTGTGATGGTTGTCTCAGCCAAGTTAATGTGTCATCTCATACTAATGCTGAAAACTGATCCGACTACCTATTTTTTCAAA
TTGGTTGTAGCAGCCTCAAGCTACCTGGATTATAAATTATTTTTTTGATGACAAAAGAAATATAAAAAAATATATTCCAC
GTAGCCTGGGGCTGCTACAATCAATTTGTAATTCTTGAAATAGTTAGTCAAATTGGGTTTCTCATATTCCATAATCTTGC
TAATCCAATCTGTAAAATGCAAATAACTTTTCAATTTGTAATTACAGGACGAAAAGGTGTGAAAGGTGCACAGCCAGGTC
CAGTATTATCATGGGCCCAACGGGTTAAAATTGCCGTTGGAGCTGCAAAAGGGCTAGAGTACTTGCATGAAAAAGCACAA
CCACATATTATCCATCGTGATATTAAGTCTAGCAATGTCCTACTCTTTGATGATGATGTTGCTAAGATTGCGGATTTTGA
TTTATCAAATCAAGCTCCTGATATGGCAGCACGCCTTCACTCCACACGTGTTCTTGGAACCTTTGGTTATCATGCTCCTG
AGTGAGTTCACTTCTTAATATTTGATACAAATAGACTGTAAACTTGGTTAACTCACTTAAATGAAGCAATTTTAGCTCAG
GGCCCAAAGGTCCTTCATTTTTATGCTGAATGGACTTTGCTCATGTTGCAGATATGCAATGACTGGTCAGCTGAGTTCAA
AGAGTGATGTTTACAGCTTTGGTGTTGTCCTCCTTGAACTACTCACTGGTCGTAAACCTGTTGATCATACATTACCACGT
GGCCAACAGAGTCTTGTGACATGGGTATAACCCTCAAGATACTGACATTACTATAACTGATGCACCAATTTTTTAGTTGA
TTATTTTGATTCTGAATTGACTTTCTGCACCATGTGTAATCAGGCAACACCGAGACTTAGTGAAGATAAAGTGAAGCAAT
GTGTTGACGCTAGACTCGGTACAGATTACCCTCCTAAGGCCATTGCAAAGGTATGTCACTAGAGCAGAAAATTCTTGACT
ACTAAAATCATAATCCTGCATCTTCTTACATCTCTTCTTGATTTTTCATCAATTCCTATTTCTCAAATTTTGTGTTGCTG
AAACAGATGGCTGCTGTTGCTGCTTTGTGTGTGCAATACGAAGCTGATTTTCGGCCAAATATGAGCATTGTGGTAAAAGC
TCTACAACCTCTTTTGCACGCTCGACCTGCACCTAGTGAAACATCAAACTTGTGATTCTCTCCTTTGCCGCAGCCGCTAC
CAAATGGAATGACAGTGATTGAGATTTGTTTTGTTGAGTTGAAAATGATATTATACTAGTAGTTATCTGGGTAAATAGCA
GACATAATGTGCTTTCCCCCCTTCTTTTACTGGTCTCAATTGAATGTGAATAAATTTATACATGTACTAATGAAAGTAGC
CTTTGCCTATTCTTTTAATGGATATTATTCTTGAGGTCTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 TCAAGACCTCAAGAATAATATCCATTAAAAGAATAGGCAAAGGCTACTTT 50
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TCAAGACCTCAAGAATAATATCCATTAAAAGAATAGGCAAAGGCTACTTT 50
Wildtype 51 CATTAGTACATGTATAAATTTATTCACATTCAATTGAGACCAGTAAAAGA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CATTAGTACATGTATAAATTTATTCACATTCAATTGAGACCAGTAAAAGA 100
Wildtype 101 AGGGGGGAAAGCACATTATGTCTGCTATTTACCCAGATAACTACTAGTAT 150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 AGGGGGGAAAGCACATTATGTCTGCTATTTACCCAGATAACTACTAGTAT 150
Wildtype 151 AATATCATTTTCAACTCAACAAAACAAATCTCAATCACTGTCATTCCATT 200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 AATATCATTTTCAACTCAACAAAACAAATCTCAATCACTGTCATTCCATT 200
Wildtype 201 TGGTAGCGGCTGCGGCAAAGGAGAGAATCACAAGTTTGATGTTTCACTAG 250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TGGTAGCGGCTGCGGCAAAGGAGAGAATCACAAGTTTGATGTTTCACTAG 250
Wildtype 251 GTGCAGGTCGAGCGTGCAAAAGAGGTTGTAGAGCTTTTACCACAATGCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GTGCAGGTCGAGCGTGCAAAAGAGGTTGTAGAGCTTTTACCACAATGCTC 300
Wildtype 301 ATATTTGGCCGAAAATCAGCTTCGTATTGCACACACAAAGCAGCAACAGC 350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 ATATTTGGCCGAAAATCAGCTTCGTATTGCACACACAAAGCAGCAACAGC 350
Wildtype 351 AGCCATCTGTTTCAGCAACACAAAATTTGAGAAATAGGAATTGATGAAAA 400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AGCCATCTGTTTCAGCAACACAAAATTTGAGAAATAGGAATTGATGAAAA 400
Wildtype 401 ATCAAGAAGAGATGTAAGAAGATGCAGGATTATGATTTTAGTAGTCAAGA 450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCAAGAAGAGATGTAAGAAGATGCAGGATTATGATTTTAGTAGTCAAGA 450
Wildtype 451 ATTTTCTGCTCTAGTGACATACCTTTGCAATGGCCTTAGGAGGGTAATCT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 ATTTTCTGCTCTAGTGACATACCTTTGCAATGGCCTTAGGAGGGTAATCT 500
Wildtype 501 GTACCGAGTCTAGCGTCAACACATTGCTTCACTTTATCTTCACTAAGTCT 550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 GTACCGAGTCTAGCGTCAACACATTGCTTCACTTTATCTTCACTAAGTCT 550
Wildtype 551 CGGTGTTGCCTGATTACACATGGTGCAGAAAGTCAATTCAGAATCAAAAT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 CGGTGTTGCCTGATTACACATGGTGCAGAAAGTCAATTCAGAATCAAAAT 600
Wildtype 601 AATCAACTAAAAAATTGGTGCATCAGTTATAGTAATGTCAGTATCTTGAG 650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AATCAACTAAAAAATTGGTGCATCAGTTATAGTAATGTCAGTATCTTGAG 650
Wildtype 651 GGTTATACCCATGTCACAAGACTCTGTTGGCCACGTGGTAATGTATGATC 700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GGTTATACCCATGTCACAAGACTCTGTTGGCCACGTGGTAATGTATGATC 700
Wildtype 701 AACAGGTTTACGACCAGTGAGTAGTTCAAGGAGGACAACACCAAAGCTGT 750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 701 AACAGGTTTACGACCAGTGAGTAGTTCAAGGAGGACAACACCAAAGCTGT 750
Wildtype 751 AAACATCACTCTTTGAACTCAGCTGACCAGTCATTGCATATCTGCAACAT 800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 751 AAACATCACTCTTTGAACTCAGCTGACCAGTCATTGCATATCTGCAACAT 800
Wildtype 801 GAGCAAAGTCCATTCAGCATAAAAATGAAGGACCTTTGGGCCCTGAGCTA 850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 801 GAGCAAAGTCCATTCAGCATAAAAATGAAGGACCTTTGGGCCCTGAGCTA 850
Wildtype 851 AAATTGCTTCATTTAAGTGAGTTAACCAAGTTTACAGTCTATTTGTATCA 900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 851 AAATTGCTTCATTTAAGTGAGTTAACCAAGTTTACAGTCTATTTGTATCA 900
Wildtype 901 AATATTAAGAAGTGAACTCACTCAGGAGCATGATAACCAAAGGTTCCAAG 950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 901 AATATTAAGAAGTGAACTCACTCAGGAGCATGATAACCAAAGGTTCCAAG 950
Wildtype 951 AACACGTGTGGAGTGAAGGCGTGCTGCCATATCAGGAGCTTGATTTGATA 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 951 AACACGTGTGGAGTGAAGGCGTGCTGCCATATCAGGAGCTTGATTTGATA 1000
Wildtype 1001 AATCAAAATCCGCAATCTTAGCAACATCATCATCAAAGAGTAGGACATTG 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1001 AATCAAAATCCGCAATCTTAGCAACATCATCATCAAAGAGTAGGACATTG 1050
Wildtype 1051 CTAGACTTAATATCACGATGGATAATATGTGGTTGTGCTTTTTCATGCAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1051 CTAGACTTAATATCACGATGGATAATATGTGGTTGTGCTTTTTCATGCAA 1100
Wildtype 1101 GTACTCTAGCCCTTTTGCAGCTCCAACGGCAATTTTAACCCGTTGGGCCC 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1101 GTACTCTAGCCCTTTTGCAGCTCCAACGGCAATTTTAACCCGTTGGGCCC 1150
Wildtype 1151 ATGATAATACTGGACCTGGCTGTGCACCTTTCACACCTTTTCGTCCTGTA 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1151 ATGATAATACTGGACCTGGCTGTGCACCTTTCACACCTTTTCGTCCTGTA 1200
Wildtype 1201 ATTACAAATTGAAAAGTTATTTGCATTTTACAGATTGGATTAGCAAGATT 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1201 ATTACAAATTGAAAAGTTATTTGCATTTTACAGATTGGATTAGCAAGATT 1250
Wildtype 1251 ATGGAATATGAGAAACCCAATTTGACTAACTATTTCAAGAATTACAAATT 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1251 ATGGAATATGAGAAACCCAATTTGACTAACTATTTCAAGAATTACAAATT 1300
Wildtype 1301 GATTGTAGCAGCCCCAGGCTACGTGGAATATATTTTTTTATATTTCTTTT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1301 GATTGTAGCAGCCCCAGGCTACGTGGAATATATTTTTTTATATTTCTTTT 1350
Wildtype 1351 GTCATCAAAAAAATAATTTATAATCCAGGTAGCTTGAGGCTGCTACAACC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1351 GTCATCAAAAAAATAATTTATAATCCAGGTAGCTTGAGGCTGCTACAACC 1400
Wildtype 1401 AATTTGAAAAAATAGGTAGTCGGATCAGTTTTCAGCATTAGTATGAGATG 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1401 AATTTGAAAAAATAGGTAGTCGGATCAGTTTTCAGCATTAGTATGAGATG 1450
Wildtype 1451 ACACATTAACTTGGCTGAGACAACCATCACAATCAATAGTTGTTCCAGAA 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1451 ACACATTAACTTGGCTGAGACAACCATCACAATCAATAGTTGTTCCAGAA 1500
Wildtype 1501 AAGTAAAAAAAATGAGAATGATCAGCCATACATAAAGAAGGAAAACACAA 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1501 AAGTAAAAAAAATGAGAATGATCAGCCATACATAAAGAAGGAAAACACAA 1550
Wildtype 1551 CAAAAACTTCCAAATAAAACAGGAATATGTATACACACGCATATGTACAG 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1551 CAAAAACTTCCAAATAAAACAGGAATATGTATACACACGCATATGTACAG 1600
Wildtype 1601 AGATAGATACCCTCGTAGTCAGAAAATAGTTCAAATAAGGAAAATTGTAA 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1601 AGATAGATACCCTCGTAGTCAGAAAATAGTTCAAATAAGGAAAATTGTAA 1650
Wildtype 1651 GAGAAAACAAAGGCTTGTTACGGAATTTGCTCTGTTAAATAGAAATACAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1651 GAGAAAACAAAGGCTTGTTACGGAATTTGCTCTGTTAAATAGAAATACAA 1700
Wildtype 1701 CATGCTGTCAGCAAATGACAACAATTTTCATTAAACAGCAATTGCACGGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1701 CATGCTGTCAGCAAATGACAACAATTTTCATTAAACAGCAATTGCACGGC 1750
Wildtype 1751 AGAAGTTTTATTTTGCTTAACATAGTGGAAATGGATAGGTAAATACAACC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1751 AGAAGTTTTATTTTGCTTAACATAGTGGAAATGGATAGGTAAATACAACC 1800
Wildtype 1801 CCAAAACATCACTTTTTGGAGCTGTTCCCATATATTAGAATTTACATATA 1850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1801 CCAAAACATCACTTTTTGGAGCTGTTCCCATATATTAGAATTTACATATA 1850
Wildtype 1851 AGGGAGAGAGAGAGAGGAAGGATGCAATTGATTTGGCAGGATAATGATGG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1851 AGGGAGAGAGAGAGAGGAAGGATGCAATTGATTTGGCAGGATAATGATGG 1900
Wildtype 1901 ACTTCCAAGATACTAGGATTTTATCCTTTTCTTTTACCTACTAACGGAGC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1901 ACTTCCAAGATACTAGGATTTTATCCTTTTCTTTTACCTACTAACGGAGC 1950
Wildtype 1951 AGTGCCAATCGCTGAAATTAACATGAGAAAAAGGTGGGTTAGACTAAATT 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1951 AGTGCCAATCGCTGAAATTAACATGAGAAAAAGGTGGGTTAGACTAAATT 2000
Wildtype 2001 TTGAAGAAAAAGAAGAGCATCTTACGCGACTGCAATCAAATTCAATTACA 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2001 TTGAAGAAAAAGAAGAGCATCTTACGCGACTGCAATCAAATTCAATTACA 2050
Wildtype 2051 TTGGGTCAAAATATATAGGAACAACCATCTCCGTATAAAGCTTCATCAGT 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2051 TTGGGTCAAAATATATAGGAACAACCATCTCCGTATAAAGCTTCATCAGT 2100
Wildtype 2101 ATGATGTAGAAACCCACTTCAAGCTCTCTTCCTCTCCCTACTTCCTTGAT 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2101 ATGATGTAGAAACCCACTTCAAGCTCTCTTCCTCTCCCTACTTCCTTGAT 2150
Wildtype 2151 AGATTATCCTTCCGATATCTGATAAATGAGGTGGGCCAATCGCAATGACC 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2151 AGATTATCCTTCCGATATCTGATAAATGAGGTGGGCCAATCGCAATGACC 2200
Wildtype 2201 TTAGTGACAGCAGTCTTTCCAAGTTTGGACTATCAAAAGGCTACTAGGGG 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2201 TTAGTGACAGCAGTCTTTCCAAGTTTGGACTATCAAAAGGCTACTAGGGG 2250
Wildtype 2251 AATGGAAGCACACTAAAAATAATAATATTCTACCTTCAGATATCCCGTAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2251 AATGGAAGCACACTAAAAATAATAATATTCTACCTTCAGATATCCCGTAA 2300
Wildtype 2301 ACCCTATATCTTAACCCAGTGCACACTTCCTGTCCAATTTTACACGTCCA 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2301 ACCCTATATCTTAACCCAGTGCACACTTCCTGTCCAATTTTACACGTCCA 2350
Wildtype 2351 AAATGTTTAACACAGTCTTGAACCAGATTGGACAGAATTATACATTGAAA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2351 AAATGTTTAACACAGTCTTGAACCAGATTGGACAGAATTATACATTGAAA 2400
Wildtype 2401 CCCAATATACCAAAATCTGGATCCAAGTAGGTTAGGTTAAAGCAAGCATG 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2401 CCCAATATACCAAAATCTGGATCCAAGTAGGTTAGGTTAAAGCAAGCATG 2450
Wildtype 2451 AGTTCAAACCCCTATTGTCATTAGGATTAATAGCTACTCAATCTGAGGAA 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2451 AGTTCAAACCCCTATTGTCATTAGGATTAATAGCTACTCAATCTGAGGAA 2500
Wildtype 2501 CTTAAAACTTATATGTAGACATTTTCCATTTTAACAACTTATCATTTTCA 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2501 CTTAAAACTTATATGTAGACATTTTCCATTTTAACAACTTATCATTTTCA 2550
Wildtype 2551 GAAGTCTGTCCAGTCACCACCAATATAATATGTAGAAAACTTTTTCACTA 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2551 GAAGTCTGTCCAGTCACCACCAATATAATATGTAGAAAACTTTTTCACTA 2600
Wildtype 2601 TATGCACAGTCAAGTACGTAACAAATGGAACTTATGAATGAAATATATAT 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2601 TATGCACAGTCAAGTACGTAACAAATGGAACTTATGAATGAAATATATAT 2650
Wildtype 2651 TTGAGAAGAGGACAAGAGAAAGAAATGTATATGCACAACAGAGAAGAAAA 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2651 TTGAGAAGAGGACAAGAGAAAGAAATGTATATGCACAACAGAGAAGAAAA 2700
Wildtype 2701 GGATAAAATCTGAAATTAAGAAGGAGATACTTAAGGGTTTGTCCAATTAG 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2701 GGATAAAATCTGAAATTAAGAAGGAGATACTTAAGGGTTTGTCCAATTAG 2750
Wildtype 2751 TAGCATAAAATCTGAGGAAGTGTTGGTGCAAGTCTACTCTTGATTAGAGC 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2751 TAGCATAAAATCTGAGGAAGTGTTGGTGCAAGTCTACTCTTGATTAGAGC 2800
Wildtype 2801 AACTTTACATGACACTTCCATGACACATGTCAAAAAAGACCCTGGTGAGG 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2801 AACTTTACATGACACTTCCATGACACATGTCAAAAAAGACCCTGGTGAGG 2850
Wildtype 2851 TACTTCAAGAAAGGGGATATATTGGGGGAGGAGCTTTGACAACTTCTTGA 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2851 TACTTCAAGAAAGGGGATATATTGGGGGAGGAGCTTTGACAACTTCTTGA 2900
Wildtype 2901 GGGTGCAGTAGTCAAGTACTTAAAGTGCACACTATAGGGAAGGCGCATGA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2901 GGGTGCAGTAGTCAAGTACTTAAAGTGCACACTATAGGGAAGGCGCATGA 2950
Wildtype 2951 CCGAGTTGGATTTGGATTGCGCAATCATTTCCAAACTCAACGTACCAAGA 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2951 CCGAGTTGGATTTGGATTGCGCAATCATTTCCAAACTCAACGTACCAAGA 3000
Wildtype 3001 TGGATATAAATTAGAACAACCAAATCTGAATTTCACCGGTATATATAGAT 3050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3001 TGGATATAAATTAGAACAACCAAATCTGAATTTCACCGGTATATATAGAT 3050
Wildtype 3051 TAGAACAACCAAATCATTAATTGGTCAACATGCATAGAATGGATACACAA 3100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3051 TAGAACAACCAAATCATTAATTGGTCAACATGCATAGAATGGATACACAA 3100
Wildtype 3101 AGGCTTATAGAAACTAACCATGAAGAATGTCATGGAGAGATCCATTGGGG 3150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3101 AGGCTTATAGAAACTAACCATGAAGAATGTCATGGAGAGATCCATTGGGG 3150
Wildtype 3151 GCATACTCATAAGCCAGCACACGAAGACCGCCATCCACACTATAACCGAG 3200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3151 GCATACTCATAAGCCAGCACACGAAGACCGCCATCCACACTATAACCGAG 3200
Wildtype 3201 TAACTCAACCACGTTTTCATGTTTTAGTCTTGATACCATGGAAACCTGAA 3250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3201 TAACTCAACCACGTTTTCATGTTTTAGTCTTGATACCATGGAAACCTGAA 3250
Wildtype 3251 ATGGTAACATCATATCCAAGCAAGGTAAGAGGTTTATGTATAATATAGAA 3300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3251 ATGGTAACATCATATCCAAGCAAGGTAAGAGGTTTATGTATAATATAGAA 3300
Wildtype 3301 TAGTTCAAAGTAGGACAATCCAAACGACCTGACCTGTGCTGAAAATTCTC 3350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3301 TAGTTCAAAGTAGGACAATCCAAACGACCTGACCTGTGCTGAAAATTCTC 3350
Wildtype 3351 GATCAGGTTGCTTGCTCGAGTCTAACTTTTTAATGGCTGCAGCCCGCCCA 3400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3351 GATCAGGTTGCTTGCTCGAGTCTAACTTTTTAATGGCTGCAGCCCGCCCA 3400
Wildtype 3401 CTTTTCAGGACACCATGGTATACCCTTCCATACGATCCCTCACCTATCAA 3450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3401 CTTTTCAGGACACCATGGTATACCCTTCCATACGATCCCTCACCTATCAA 3450
Wildtype 3451 GGCTTTTGTACCAAAGTTGTCTGTGATGTCTTTTAACTCATCAACAGTTA 3500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3451 GGCTTTTGTACCAAAGTTGTCTGTGATGTCTTTTAACTCATCAACAGTTA 3500
Wildtype 3501 TAGAAGGAACAGCAATGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGC 3550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3501 TAGAAGGAACAGCAATGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGC 3550
gRNA TTGC
Wildtype 3551 GCACTTTCTGTTACACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCC 3600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3551 GCACTTTCTGTTACACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCC 3600
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3601 AGTTAGTGAAAACCATAAATCGTACAGGTAAAAAAAAGAATTAATAGAAT 3650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3601 AGTTAGTGAAAACCATAAATCGTACAGGTAAAAAAAAGAATTAATAGAAT 3650
Wildtype 3651 CTCAGCGAGTAAAATCATGACACTATCTAAAGAAAGCATATATTTAGGAT 3700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3651 CTCAGCGAGTAAAATCATGACACTATCTAAAGAAAGCATATATTTAGGAT 3700
Wildtype 3701 AGGCGTAAAGCCAAAGCAATGTACTAAACACTACAACTTTCACCTGACTG 3750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3701 AGGCGTAAAGCCAAAGCAATGTACTAAACACTACAACTTTCACCTGACTG 3750
Wildtype 3751 TTTCAAAATTTACCAAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTT 3800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3751 TTTCAAAATTTACCAAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTT 3800
Wildtype 3801 TGCAGAGCAGGCATATCTCCCATACCAGGTCACAACGACACTAGATTCAA 3850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3801 TGCAGAGCAGGCATATCTCCCATACCAGGTCACAACGACACTAGATTCAA 3850
Wildtype 3851 ACATCATGATCTCTTAACTTCCAAGATACTTGCACTAATTTACTTATAGA 3900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3851 ACATCATGATCTCTTAACTTCCAAGATACTTGCACTAATTTACTTATAGA 3900
Wildtype 3901 CCTGATAGGAAGATTTGCCTATTAGTTCCCAACTCTACTGAGTACTGATA 3950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3901 CCTGATAGGAAGATTTGCCTATTAGTTCCCAACTCTACTGAGTACTGATA 3950
Wildtype 3951 ATGTATTATACATCAGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGA 4000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3951 ATGTATTATACATCAGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGA 4000
Wildtype 4001 GATACTAGGATTGAAACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAA 4050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4001 GATACTAGGATTGAAACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAA 4050
Wildtype 4051 TTTATTATTTCAAAGAGTCTGAAAATATTATTAATAGCAAATAGTACAAG 4100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4051 TTTATTATTTCAAAGAGTCTGAAAATATTATTAATAGCAAATAGTACAAG 4100
Wildtype 4101 GAGGAAATTAACCTGCTGCATTGTTGGTCATGAATGGTCCATGATCAGCA 4150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4101 GAGGAAATTAACCTGCTGCATTGTTGGTCATGAATGGTCCATGATCAGCA 4150
Wildtype 4151 GGTTTATGCATGTCATCATCGTCACAACAACCGAAGCAGCTCATAGTTAT 4200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4151 GGTTTATGCATGTCATCATCGTCACAACAACCGAAGCAGCTCATAGTTAT 4200
Wildtype 4201 CC 4202
||
MutationRev 4201 CC 4202
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-4 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
Immunity response: More susceptible to DC3000dd; decrease in ROS response to csp22
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-4-1 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-4-2 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-4-3 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-4-4 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-8 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
Immunity response: "No significant change in resistance to DC3000dd, ROS not tested"
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-8-1 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0005 |
Moneymaker |
CTG0005-2 |
CGR0005-2 |
CRT0005-4-8-2 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TGATCATGGACCATTCATGACCAACAATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGA
CTCTTTGAAATAATAAATTCAGCTGCAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAG
CTCAACCTTAACCACTGAATACTGATGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCC
TATCAGGTCTATAAGTAAATTAGTGCAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACC
TGGTATGGGAGATATGCCTGCTCTGCAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAG
TCAGGTGAAAGTTGTAGTGTTTAGTACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCA
TGATTTTACTCGCTGAGATTCTATTAATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTT
TAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCGTCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGT
TCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATG
GAAGGGTATACCA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3416 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 3465
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TGGTATACCCTTCCATACGATCCCTCACCTATCAAGGCTTTTGTACCAAA 50
Wildtype 3466 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 3515
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GTTGTCTGTGATGTCTTTTAACTCATCAACAGTTATAGAAGGAACAGCAA 100
Wildtype 3516 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTG-CGCACTTTCTGTTAC 3564
|||||||||||||||||||||||||||||||||| |||||||||||||||
MutationRev 101 TGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGACGCACTTTCTGTTAC 150
gRNA TTG-CGCACTTTCTGTTAC
Wildtype 3565 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 3614
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCCAGTTAGTGAAAACC 200
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3615 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 3664
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 ATAAATCGTACAGGTAAAAAAAAGAATTAATAGAATCTCAGCGAGTAAAA 250
Wildtype 3665 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 3714
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCATGACACTATCTAAAGAAAGCATATATTTAGGATAGGCGTAAAGCCAA 300
Wildtype 3715 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 3764
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGCAATGTACTAAACACTACAACTTTCACCTGACTGTTTCAAAATTTACC 350
Wildtype 3765 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 3814
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTTTGCAGAGCAGGCAT 400
Wildtype 3815 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 3864
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCTCCCATACCAGGTCACAACGACACTAGATTCAAACATCATGATCTCT 450
Wildtype 3865 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 3914
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TAACTTCCAAGATACTTGCACTAATTTACTTATAGACCTGATAGGAAGAT 500
Wildtype 3915 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 3964
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 TTGCCTATTAGTTCCCAACTCTACTGAGTACTGATAATGTATTATACATC 550
Wildtype 3965 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 4014
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 AGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGAGATACTAGGATTGA 600
Wildtype 4015 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 4064
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAATTTATTATTTCAAA 650
Wildtype 4065 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 4114
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GAGTCTGAAAATATTATTAATAGCAAATAGTACAAGGAGGAAATTAACCT 700
Wildtype 4115 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 4147
|||||||||||||||||||||||||||||||||
MutationRev 701 GCTGCATTGTTGGTCATGAATGGTCCATGATCA 733
|
|
CRT0006 |
RG-prf3 |
CTG0005-2 |
CGR0005-2 |
CRT0006-15 (T0 ) |
heterozygous
|
|
CRT0006 |
RG-prf3 |
CTG0005-2 |
CGR0005-2 |
CRT0006-15-10 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: GGATAACTATGAGCTGCTTCGGTTGTTGTGACGATGATGACATGCATAAACCTGCTGATCATGGACCATTCATGACCAAC
AATGCAGCAGGTTAATTTCCTCCTTGTACTATTTGCTATTAATAATATTTTCAGACTCTTTGAAATAATAAATTCAGCTG
CAGTAGAGGAGCAAGAGGGTAGTGGGTTTCAATCCTAGTATCTCCTGGATTAAAGCTCAACCTTAACCACTGAATACTGA
TGTATAATACATTATCAGTACTCAGTAGAGTTGGGAACTAATAGGCAAATCTTCCTATCAGGTCTATAAGTAAATTAGTG
CAAGTATCTTGGAAGTTAAGAGATCATGATGTTTGAATCTAGTGTCGTTGTGACCTGGTATGGGAGATATGCCTGCTCTG
CAAACATGTCTTTCACCTCAATCTTACAGTTTAACCTTGGTAAATTTTGAAACAGTCAGGTGAAAGTTGTAGTGTTTAGT
ACATTGCTTTGGCTTTACGCCTATCCTAAATATATGCTTTCTTTAGATAGTGTCATGATTTTACTCGCTGAGATTCTATT
AATTCTTTTTTTTACCTGTACGATTTATGGTTTTCACTAACTGGAAATCTTTTTTTAGGCTACAATGCAGGGCAGCGTGT
AACAGAAAGTGCGCAAAGGGAGACGCAGAATGTTAACATCCTGCCCATTGCTGTTCCTTCTATAACTGTTGATGAGTTAA
AAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGGGATCGTATGGAAGGGTATACCATGGTGTCCTGAAA
AGTGGGCGGGCTGCAGCCATTAAAAAGTTAGACTCGAGCAAGCAACCTGATCGAGAATTTTCAGCACAGGTCAGGTCGTT
TGGATTGTCCTACTTTGAACTATTCTATATTATACATAAACCTCTTACCTTGCTTGGATATGATGTTACCATTTCAGGTT
TCCATGGTATCAAGACTAAAACATGAAAACGTGGTTGAGTTACTCGGTTATAGTGTGGATGGCGGTCTTCGTGTGCTGGC
TTATGAGTATGCCCCCAATGGATCTCTCCATGACATTCTTCATGGTTAGTTTCTATAAGCCTTTGTGTATCCATTCTATG
CATGTTGACCAATTAATGATTTGGTTGTTCTAATCTATATATACCGGTGAAATTCAGATTTGGTTGTTCTAATTTATATC
CATCTTGGTACGTTGAGTTTGGAAATGATTGCGCAATCCAAATCCAACTCGGTCATGCGCCTTCCCTATAGTGTGCACTT
TAAGTACTTGACTACTGCACCCTCAAGAAGTTGTCAAAGCTCCTCCCCCAATATATCCCCTTTCTTGAAGTACCTCACCA
GGGTCTTTTTTGACATGTGTCATGGAAGTGTCATGTAAAGTTGCTCTAATCAAGAGTAGACTTGCACCAACACTTCCTCA
GATTTTATGCTACTAATTGGACAAACCCTTAAGTATCTCCTTCTTAATTTCAGATTTTATCCTTTTCTTCTCTGTTGTGC
ATATACATTTCTTTCTCTTGTCCTCTTCTCAAATATATATTTCATTCATAAGTTCCATTTGTTACGTACTTGACTGTGCA
TATAGTGAAAAAGTTTTCTACATATTATATTGGTGGTGACTGGACAGACTTCTGAAAATGATAAGTTGTTAAAATGGAAA
ATGTCTACATATAAGTTTTAAGTTCCTCAGATTGAGTAGCTATTAATCCTAATGACAATAGGGGTTTGAACTCATGCTTG
CTTTAACCTAACCTACTTGGATCCAGATTTTGGTATATTGGGTTTCAATGTATAATTCTGTCCAATCTGGTTCAAGACTG
TGTTAAACATTTTGGACGTGTAAAATTGGACAGGAAGTGTGCACTGGGTTAAGATATAGGGTTTACGGGATATCTGAAGG
TAGAATATTATTATTTTTAGTGTGCTTCCATTCCCCTAGTAGCCTTTTGATAGTCCAAACTTGGAAAGACTGCTGTCACT
AAGGTCATTGCGATTGGCCCACCTCATTTATCAGATATCGGAAGGATAATCTATCAAGGAAGTAGGGAGAGGAAGAGAGC
TTGAAGTGGGTTTCTACATCATACTGATGAAGCTTTATACGGAGATGGTTGTTCCTATATATTTTGACCCAATGTAATTG
AATTTGATTGCAGTCGCGTAAGATGCTCTTCTTTTTCTTCAAAATTTAGTCTAACCCACCTTTTTCTCATGTTAATTTCA
GCGATTGGCACTGCTCCGTTAGTAGGTAAAAGAAAAGGATAAAATCCTAGTATCTTGGAAGTCCATCATTATCCTGCCAA
ATCAATTGCATCCTTCCTCTCTCTCTCTCCCTTATATGTAAATTCTAATATATGGGAACAGCTCCAAAAAGTGATGTTTT
GGGGTTGTATTTACCTATCCATTTCCACTATGTTAAGCAAAATAAAACTTCTGCCGTGCAATTGCTGTTTAATGAAAATT
GTTGTCATTTGCTGACAGCATGTTGTATTTCTATTTAACAGAGCAAATTCCGTAACAAGCCTTTGTTTTCTCTTACAATT
TTCCTTATTTGAACTATTTTCTGACTACGAGGGTATCTATCTCTGTACATATGCGTGTGTATACATATTCCTGTTTTATT
TGGAAGTTTTTGTTGTGTTTTCCTTCTTTATGTATGGCTGATCATTCTCATTTTTTTTACTTTTCTGGAACAACTATTGA
TTGTGATGGTTGTCTCAGCCAAGTTAATGTGTCATCTCATACTAATGCTGAAAACTGATCCGACTACCTATTTTTTCAAA
TTGGTTGTAGCAGCCTCAAGCTACCTGGATTATAAATTATTTTTTTGATGACAAAAGAAATATAAAAAAATATATTCCAC
GTAGCCTGGGGCTGCTACAATCAATTTGTAATTCTTGAAATAGTTAGTCAAATTGGGTTTCTCATATTCCATAATCTTGC
TAATCCAATCTGTAAAATGCAAATAACTTTTCAATTTGTAATTACAGGACGAAAAGGTGTGAAAGGTGCACAGCCAGGTC
CAGTATTATCATGGGCCCAACGGGTTAAAATTGCCGTTGGAGCTGCAAAAGGGCTAGAGTACTTGCATGAAAAAGCACAA
CCACATATTATCCATCGTGATATTAAGTCTAGCAATGTCCTACTCTTTGATGATGATGTTGCTAAGATTGCGGATTTTGA
TTTATCAAATCAAGCTCCTGATATGGCAGCACGCCTTCACTCCACACGTGTTCTTGGAACCTTTGGTTATCATGCTCCTG
AGTGAGTTCACTTCTTAATATTTGATACAAATAGACTGTAAACTTGGTTAACTCACTTAAATGAAGCAATTTTAGCTCAG
GGCCCAAAGGTCCTTCATTTTTATGCTGAATGGACTTTGCTCATGTTGCAGATATGCAATGACTGGTCAGCTGAGTTCAA
AGAGTGATGTTTACAGCTTTGGTGTTGTCCTCCTTGAACTACTCACTGGTCGTAAACCTGTTGATCATACATTACCACGT
GGCCAACAGAGTCTTGTGACATGGGTATAACCCTCAAGATACTGACATTACTATAACTGATGCACCAATTTTTTAGTTGA
TTATTTTGATTCTGAATTGACTTTCTGCACCATGTGTAATCAGGCAACACCGAGACTTAGTGAAGATAAAGTGAAGCAAT
GTGTTGACGCTAGACTCGGTACAGATTACCCTCCTAAGGCCATTGCAAAGGTATGTCACTAGAGCAGAAAATTCTTGACT
ACTAAAATCATAATCCTGCATCTTCTTACATCTCTTCTTGATTTTTCATCAATTCCTATTTCTCAAATTTTGTGTTGCTG
AAACAGATGGCTGCTGTTGCTGCTTTGTGTGTGCAATACGAAGCTGATTTTCGGCCAAATATGAGCATTGTGGTAAAAGC
TCTACAACCTCTTTTGCACGCTCGACCTGCACCTAGTGAAACATCAAACTTGTGATTCTCTCCTTTGCCGCAGCCGCTAC
CAAATGGAATGACAGTGATTGAGATTTGTTTTGTTGAGTTGAAAATGATATTATACTAGTAGTTATCTGGGTAAATAGCA
GACATAATGTGCTTTCCCCCCTTCTTTTACTGGTCTCAATTGAATGTGAATAAATTTATACATGTACTAATGAAAGTAGC
CTTTGCCTATTCTTTTAATGGATATTATTCTTGAGGTCTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 TCAAGACCTCAAGAATAATATCCATTAAAAGAATAGGCAAAGGCTACTTT 50
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TCAAGACCTCAAGAATAATATCCATTAAAAGAATAGGCAAAGGCTACTTT 50
Wildtype 51 CATTAGTACATGTATAAATTTATTCACATTCAATTGAGACCAGTAAAAGA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CATTAGTACATGTATAAATTTATTCACATTCAATTGAGACCAGTAAAAGA 100
Wildtype 101 AGGGGGGAAAGCACATTATGTCTGCTATTTACCCAGATAACTACTAGTAT 150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 AGGGGGGAAAGCACATTATGTCTGCTATTTACCCAGATAACTACTAGTAT 150
Wildtype 151 AATATCATTTTCAACTCAACAAAACAAATCTCAATCACTGTCATTCCATT 200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 AATATCATTTTCAACTCAACAAAACAAATCTCAATCACTGTCATTCCATT 200
Wildtype 201 TGGTAGCGGCTGCGGCAAAGGAGAGAATCACAAGTTTGATGTTTCACTAG 250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TGGTAGCGGCTGCGGCAAAGGAGAGAATCACAAGTTTGATGTTTCACTAG 250
Wildtype 251 GTGCAGGTCGAGCGTGCAAAAGAGGTTGTAGAGCTTTTACCACAATGCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GTGCAGGTCGAGCGTGCAAAAGAGGTTGTAGAGCTTTTACCACAATGCTC 300
Wildtype 301 ATATTTGGCCGAAAATCAGCTTCGTATTGCACACACAAAGCAGCAACAGC 350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 ATATTTGGCCGAAAATCAGCTTCGTATTGCACACACAAAGCAGCAACAGC 350
Wildtype 351 AGCCATCTGTTTCAGCAACACAAAATTTGAGAAATAGGAATTGATGAAAA 400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 AGCCATCTGTTTCAGCAACACAAAATTTGAGAAATAGGAATTGATGAAAA 400
Wildtype 401 ATCAAGAAGAGATGTAAGAAGATGCAGGATTATGATTTTAGTAGTCAAGA 450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 ATCAAGAAGAGATGTAAGAAGATGCAGGATTATGATTTTAGTAGTCAAGA 450
Wildtype 451 ATTTTCTGCTCTAGTGACATACCTTTGCAATGGCCTTAGGAGGGTAATCT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 ATTTTCTGCTCTAGTGACATACCTTTGCAATGGCCTTAGGAGGGTAATCT 500
Wildtype 501 GTACCGAGTCTAGCGTCAACACATTGCTTCACTTTATCTTCACTAAGTCT 550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 GTACCGAGTCTAGCGTCAACACATTGCTTCACTTTATCTTCACTAAGTCT 550
Wildtype 551 CGGTGTTGCCTGATTACACATGGTGCAGAAAGTCAATTCAGAATCAAAAT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 CGGTGTTGCCTGATTACACATGGTGCAGAAAGTCAATTCAGAATCAAAAT 600
Wildtype 601 AATCAACTAAAAAATTGGTGCATCAGTTATAGTAATGTCAGTATCTTGAG 650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 AATCAACTAAAAAATTGGTGCATCAGTTATAGTAATGTCAGTATCTTGAG 650
Wildtype 651 GGTTATACCCATGTCACAAGACTCTGTTGGCCACGTGGTAATGTATGATC 700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 GGTTATACCCATGTCACAAGACTCTGTTGGCCACGTGGTAATGTATGATC 700
Wildtype 701 AACAGGTTTACGACCAGTGAGTAGTTCAAGGAGGACAACACCAAAGCTGT 750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 701 AACAGGTTTACGACCAGTGAGTAGTTCAAGGAGGACAACACCAAAGCTGT 750
Wildtype 751 AAACATCACTCTTTGAACTCAGCTGACCAGTCATTGCATATCTGCAACAT 800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 751 AAACATCACTCTTTGAACTCAGCTGACCAGTCATTGCATATCTGCAACAT 800
Wildtype 801 GAGCAAAGTCCATTCAGCATAAAAATGAAGGACCTTTGGGCCCTGAGCTA 850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 801 GAGCAAAGTCCATTCAGCATAAAAATGAAGGACCTTTGGGCCCTGAGCTA 850
Wildtype 851 AAATTGCTTCATTTAAGTGAGTTAACCAAGTTTACAGTCTATTTGTATCA 900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 851 AAATTGCTTCATTTAAGTGAGTTAACCAAGTTTACAGTCTATTTGTATCA 900
Wildtype 901 AATATTAAGAAGTGAACTCACTCAGGAGCATGATAACCAAAGGTTCCAAG 950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 901 AATATTAAGAAGTGAACTCACTCAGGAGCATGATAACCAAAGGTTCCAAG 950
Wildtype 951 AACACGTGTGGAGTGAAGGCGTGCTGCCATATCAGGAGCTTGATTTGATA 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 951 AACACGTGTGGAGTGAAGGCGTGCTGCCATATCAGGAGCTTGATTTGATA 1000
Wildtype 1001 AATCAAAATCCGCAATCTTAGCAACATCATCATCAAAGAGTAGGACATTG 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1001 AATCAAAATCCGCAATCTTAGCAACATCATCATCAAAGAGTAGGACATTG 1050
Wildtype 1051 CTAGACTTAATATCACGATGGATAATATGTGGTTGTGCTTTTTCATGCAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1051 CTAGACTTAATATCACGATGGATAATATGTGGTTGTGCTTTTTCATGCAA 1100
Wildtype 1101 GTACTCTAGCCCTTTTGCAGCTCCAACGGCAATTTTAACCCGTTGGGCCC 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1101 GTACTCTAGCCCTTTTGCAGCTCCAACGGCAATTTTAACCCGTTGGGCCC 1150
Wildtype 1151 ATGATAATACTGGACCTGGCTGTGCACCTTTCACACCTTTTCGTCCTGTA 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1151 ATGATAATACTGGACCTGGCTGTGCACCTTTCACACCTTTTCGTCCTGTA 1200
Wildtype 1201 ATTACAAATTGAAAAGTTATTTGCATTTTACAGATTGGATTAGCAAGATT 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1201 ATTACAAATTGAAAAGTTATTTGCATTTTACAGATTGGATTAGCAAGATT 1250
Wildtype 1251 ATGGAATATGAGAAACCCAATTTGACTAACTATTTCAAGAATTACAAATT 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1251 ATGGAATATGAGAAACCCAATTTGACTAACTATTTCAAGAATTACAAATT 1300
Wildtype 1301 GATTGTAGCAGCCCCAGGCTACGTGGAATATATTTTTTTATATTTCTTTT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1301 GATTGTAGCAGCCCCAGGCTACGTGGAATATATTTTTTTATATTTCTTTT 1350
Wildtype 1351 GTCATCAAAAAAATAATTTATAATCCAGGTAGCTTGAGGCTGCTACAACC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1351 GTCATCAAAAAAATAATTTATAATCCAGGTAGCTTGAGGCTGCTACAACC 1400
Wildtype 1401 AATTTGAAAAAATAGGTAGTCGGATCAGTTTTCAGCATTAGTATGAGATG 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1401 AATTTGAAAAAATAGGTAGTCGGATCAGTTTTCAGCATTAGTATGAGATG 1450
Wildtype 1451 ACACATTAACTTGGCTGAGACAACCATCACAATCAATAGTTGTTCCAGAA 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1451 ACACATTAACTTGGCTGAGACAACCATCACAATCAATAGTTGTTCCAGAA 1500
Wildtype 1501 AAGTAAAAAAAATGAGAATGATCAGCCATACATAAAGAAGGAAAACACAA 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1501 AAGTAAAAAAAATGAGAATGATCAGCCATACATAAAGAAGGAAAACACAA 1550
Wildtype 1551 CAAAAACTTCCAAATAAAACAGGAATATGTATACACACGCATATGTACAG 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1551 CAAAAACTTCCAAATAAAACAGGAATATGTATACACACGCATATGTACAG 1600
Wildtype 1601 AGATAGATACCCTCGTAGTCAGAAAATAGTTCAAATAAGGAAAATTGTAA 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1601 AGATAGATACCCTCGTAGTCAGAAAATAGTTCAAATAAGGAAAATTGTAA 1650
Wildtype 1651 GAGAAAACAAAGGCTTGTTACGGAATTTGCTCTGTTAAATAGAAATACAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1651 GAGAAAACAAAGGCTTGTTACGGAATTTGCTCTGTTAAATAGAAATACAA 1700
Wildtype 1701 CATGCTGTCAGCAAATGACAACAATTTTCATTAAACAGCAATTGCACGGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1701 CATGCTGTCAGCAAATGACAACAATTTTCATTAAACAGCAATTGCACGGC 1750
Wildtype 1751 AGAAGTTTTATTTTGCTTAACATAGTGGAAATGGATAGGTAAATACAACC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1751 AGAAGTTTTATTTTGCTTAACATAGTGGAAATGGATAGGTAAATACAACC 1800
Wildtype 1801 CCAAAACATCACTTTTTGGAGCTGTTCCCATATATTAGAATTTACATATA 1850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1801 CCAAAACATCACTTTTTGGAGCTGTTCCCATATATTAGAATTTACATATA 1850
Wildtype 1851 AGGGAGAGAGAGAGAGGAAGGATGCAATTGATTTGGCAGGATAATGATGG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1851 AGGGAGAGAGAGAGAGGAAGGATGCAATTGATTTGGCAGGATAATGATGG 1900
Wildtype 1901 ACTTCCAAGATACTAGGATTTTATCCTTTTCTTTTACCTACTAACGGAGC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1901 ACTTCCAAGATACTAGGATTTTATCCTTTTCTTTTACCTACTAACGGAGC 1950
Wildtype 1951 AGTGCCAATCGCTGAAATTAACATGAGAAAAAGGTGGGTTAGACTAAATT 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1951 AGTGCCAATCGCTGAAATTAACATGAGAAAAAGGTGGGTTAGACTAAATT 2000
Wildtype 2001 TTGAAGAAAAAGAAGAGCATCTTACGCGACTGCAATCAAATTCAATTACA 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2001 TTGAAGAAAAAGAAGAGCATCTTACGCGACTGCAATCAAATTCAATTACA 2050
Wildtype 2051 TTGGGTCAAAATATATAGGAACAACCATCTCCGTATAAAGCTTCATCAGT 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2051 TTGGGTCAAAATATATAGGAACAACCATCTCCGTATAAAGCTTCATCAGT 2100
Wildtype 2101 ATGATGTAGAAACCCACTTCAAGCTCTCTTCCTCTCCCTACTTCCTTGAT 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2101 ATGATGTAGAAACCCACTTCAAGCTCTCTTCCTCTCCCTACTTCCTTGAT 2150
Wildtype 2151 AGATTATCCTTCCGATATCTGATAAATGAGGTGGGCCAATCGCAATGACC 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2151 AGATTATCCTTCCGATATCTGATAAATGAGGTGGGCCAATCGCAATGACC 2200
Wildtype 2201 TTAGTGACAGCAGTCTTTCCAAGTTTGGACTATCAAAAGGCTACTAGGGG 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2201 TTAGTGACAGCAGTCTTTCCAAGTTTGGACTATCAAAAGGCTACTAGGGG 2250
Wildtype 2251 AATGGAAGCACACTAAAAATAATAATATTCTACCTTCAGATATCCCGTAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2251 AATGGAAGCACACTAAAAATAATAATATTCTACCTTCAGATATCCCGTAA 2300
Wildtype 2301 ACCCTATATCTTAACCCAGTGCACACTTCCTGTCCAATTTTACACGTCCA 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2301 ACCCTATATCTTAACCCAGTGCACACTTCCTGTCCAATTTTACACGTCCA 2350
Wildtype 2351 AAATGTTTAACACAGTCTTGAACCAGATTGGACAGAATTATACATTGAAA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2351 AAATGTTTAACACAGTCTTGAACCAGATTGGACAGAATTATACATTGAAA 2400
Wildtype 2401 CCCAATATACCAAAATCTGGATCCAAGTAGGTTAGGTTAAAGCAAGCATG 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2401 CCCAATATACCAAAATCTGGATCCAAGTAGGTTAGGTTAAAGCAAGCATG 2450
Wildtype 2451 AGTTCAAACCCCTATTGTCATTAGGATTAATAGCTACTCAATCTGAGGAA 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2451 AGTTCAAACCCCTATTGTCATTAGGATTAATAGCTACTCAATCTGAGGAA 2500
Wildtype 2501 CTTAAAACTTATATGTAGACATTTTCCATTTTAACAACTTATCATTTTCA 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2501 CTTAAAACTTATATGTAGACATTTTCCATTTTAACAACTTATCATTTTCA 2550
Wildtype 2551 GAAGTCTGTCCAGTCACCACCAATATAATATGTAGAAAACTTTTTCACTA 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2551 GAAGTCTGTCCAGTCACCACCAATATAATATGTAGAAAACTTTTTCACTA 2600
Wildtype 2601 TATGCACAGTCAAGTACGTAACAAATGGAACTTATGAATGAAATATATAT 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2601 TATGCACAGTCAAGTACGTAACAAATGGAACTTATGAATGAAATATATAT 2650
Wildtype 2651 TTGAGAAGAGGACAAGAGAAAGAAATGTATATGCACAACAGAGAAGAAAA 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2651 TTGAGAAGAGGACAAGAGAAAGAAATGTATATGCACAACAGAGAAGAAAA 2700
Wildtype 2701 GGATAAAATCTGAAATTAAGAAGGAGATACTTAAGGGTTTGTCCAATTAG 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2701 GGATAAAATCTGAAATTAAGAAGGAGATACTTAAGGGTTTGTCCAATTAG 2750
Wildtype 2751 TAGCATAAAATCTGAGGAAGTGTTGGTGCAAGTCTACTCTTGATTAGAGC 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2751 TAGCATAAAATCTGAGGAAGTGTTGGTGCAAGTCTACTCTTGATTAGAGC 2800
Wildtype 2801 AACTTTACATGACACTTCCATGACACATGTCAAAAAAGACCCTGGTGAGG 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2801 AACTTTACATGACACTTCCATGACACATGTCAAAAAAGACCCTGGTGAGG 2850
Wildtype 2851 TACTTCAAGAAAGGGGATATATTGGGGGAGGAGCTTTGACAACTTCTTGA 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2851 TACTTCAAGAAAGGGGATATATTGGGGGAGGAGCTTTGACAACTTCTTGA 2900
Wildtype 2901 GGGTGCAGTAGTCAAGTACTTAAAGTGCACACTATAGGGAAGGCGCATGA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2901 GGGTGCAGTAGTCAAGTACTTAAAGTGCACACTATAGGGAAGGCGCATGA 2950
Wildtype 2951 CCGAGTTGGATTTGGATTGCGCAATCATTTCCAAACTCAACGTACCAAGA 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 2951 CCGAGTTGGATTTGGATTGCGCAATCATTTCCAAACTCAACGTACCAAGA 3000
Wildtype 3001 TGGATATAAATTAGAACAACCAAATCTGAATTTCACCGGTATATATAGAT 3050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3001 TGGATATAAATTAGAACAACCAAATCTGAATTTCACCGGTATATATAGAT 3050
Wildtype 3051 TAGAACAACCAAATCATTAATTGGTCAACATGCATAGAATGGATACACAA 3100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3051 TAGAACAACCAAATCATTAATTGGTCAACATGCATAGAATGGATACACAA 3100
Wildtype 3101 AGGCTTATAGAAACTAACCATGAAGAATGTCATGGAGAGATCCATTGGGG 3150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3101 AGGCTTATAGAAACTAACCATGAAGAATGTCATGGAGAGATCCATTGGGG 3150
Wildtype 3151 GCATACTCATAAGCCAGCACACGAAGACCGCCATCCACACTATAACCGAG 3200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3151 GCATACTCATAAGCCAGCACACGAAGACCGCCATCCACACTATAACCGAG 3200
Wildtype 3201 TAACTCAACCACGTTTTCATGTTTTAGTCTTGATACCATGGAAACCTGAA 3250
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3201 TAACTCAACCACGTTTTCATGTTTTAGTCTTGATACCATGGAAACCTGAA 3250
Wildtype 3251 ATGGTAACATCATATCCAAGCAAGGTAAGAGGTTTATGTATAATATAGAA 3300
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3251 ATGGTAACATCATATCCAAGCAAGGTAAGAGGTTTATGTATAATATAGAA 3300
Wildtype 3301 TAGTTCAAAGTAGGACAATCCAAACGACCTGACCTGTGCTGAAAATTCTC 3350
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3301 TAGTTCAAAGTAGGACAATCCAAACGACCTGACCTGTGCTGAAAATTCTC 3350
Wildtype 3351 GATCAGGTTGCTTGCTCGAGTCTAACTTTTTAATGGCTGCAGCCCGCCCA 3400
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3351 GATCAGGTTGCTTGCTCGAGTCTAACTTTTTAATGGCTGCAGCCCGCCCA 3400
Wildtype 3401 CTTTTCAGGACACCATGGTATACCCTTCCATACGATCCCTCACCTATCAA 3450
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3401 CTTTTCAGGACACCATGGTATACCCTTCCATACGATCCCTCACCTATCAA 3450
Wildtype 3451 GGCTTTTGTACCAAAGTTGTCTGTGATGTCTTTTAACTCATCAACAGTTA 3500
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3451 GGCTTTTGTACCAAAGTTGTCTGTGATGTCTTTTAACTCATCAACAGTTA 3500
Wildtype 3501 TAGAAGGAACAGCAATGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGC 3550
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3501 TAGAAGGAACAGCAATGGGCAGGATGTTAACATTCTGCGTCTCCCTTTGC 3550
gRNA TTGC
Wildtype 3551 GCACTTTCTGTTACACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCC 3600
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3551 GCACTTTCTGTTACACGCTGCCCTGCATTGTAGCCTAAAAAAAGATTTCC 3600
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3601 AGTTAGTGAAAACCATAAATCGTACAGGTAAAAAAAAGAATTAATAGAAT 3650
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3601 AGTTAGTGAAAACCATAAATCGTACAGGTAAAAAAAAGAATTAATAGAAT 3650
Wildtype 3651 CTCAGCGAGTAAAATCATGACACTATCTAAAGAAAGCATATATTTAGGAT 3700
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3651 CTCAGCGAGTAAAATCATGACACTATCTAAAGAAAGCATATATTTAGGAT 3700
Wildtype 3701 AGGCGTAAAGCCAAAGCAATGTACTAAACACTACAACTTTCACCTGACTG 3750
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3701 AGGCGTAAAGCCAAAGCAATGTACTAAACACTACAACTTTCACCTGACTG 3750
Wildtype 3751 TTTCAAAATTTACCAAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTT 3800
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3751 TTTCAAAATTTACCAAGGTTAAACTGTAAGATTGAGGTGAAAGACATGTT 3800
Wildtype 3801 TGCAGAGCAGGCATATCTCCCATACCAGGTCACAACGACACTAGATTCAA 3850
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3801 TGCAGAGCAGGCATATCTCCCATACCAGGTCACAACGACACTAGATTCAA 3850
Wildtype 3851 ACATCATGATCTCTTAACTTCCAAGATACTTGCACTAATTTACTTATAGA 3900
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3851 ACATCATGATCTCTTAACTTCCAAGATACTTGCACTAATTTACTTATAGA 3900
Wildtype 3901 CCTGATAGGAAGATTTGCCTATTAGTTCCCAACTCTACTGAGTACTGATA 3950
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3901 CCTGATAGGAAGATTTGCCTATTAGTTCCCAACTCTACTGAGTACTGATA 3950
Wildtype 3951 ATGTATTATACATCAGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGA 4000
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 3951 ATGTATTATACATCAGTATTCAGTGGTTAAGGTTGAGCTTTAATCCAGGA 4000
Wildtype 4001 GATACTAGGATTGAAACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAA 4050
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4001 GATACTAGGATTGAAACCCACTACCCTCTTGCTCCTCTACTGCAGCTGAA 4050
Wildtype 4051 TTTATTATTTCAAAGAGTCTGAAAATATTATTAATAGCAAATAGTACAAG 4100
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4051 TTTATTATTTCAAAGAGTCTGAAAATATTATTAATAGCAAATAGTACAAG 4100
Wildtype 4101 GAGGAAATTAACCTGCTGCATTGTTGGTCATGAATGGTCCATGATCAGCA 4150
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4101 GAGGAAATTAACCTGCTGCATTGTTGGTCATGAATGGTCCATGATCAGCA 4150
Wildtype 4151 GGTTTATGCATGTCATCATCGTCACAACAACCGAAGCAGCTCATAGTTAT 4200
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 4151 GGTTTATGCATGTCATCATCGTCACAACAACCGAAGCAGCTCATAGTTAT 4200
Wildtype 4201 CC 4202
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MutationRev 4201 CC 4202
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CRT0006 |
RG-prf3 |
CTG0005-2 |
CGR0005-2 |
CRT0006-15-7 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: AGACAGTGTCATGATTTTACTCACTGAGATTCTATTAATTCTTTTTTTTACCTGTAAGATTTATGGTTTTCACTAACTGG
AAATCTTTTTTTAGGCTACAATGCAGGGCAGCGTGTAACAGAAAGTGCCAAAGGGAGACGCAGAATGTTAACATCCTGCC
CATTGCTGTTCCTTCTATAACTGTTGATGAGTTAAAAGACATCACAGACAACTTTGGTACAAAAGCCTTGATAGGTGAGG
GATCGTATGGAAGGGTATACCATGGTGTCCTGAAAAGTGGGCGGG
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3393 CCCGCCCACTTTTCAGGACACCATGGTATACCCTTCCATACGATCCCTCA 3442
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 CCCGCCCACTTTTCAGGACACCATGGTATACCCTTCCATACGATCCCTCA 50
Wildtype 3443 CCTATCAAGGCTTTTGTACCAAAGTTGTCTGTGATGTCTTTTAACTCATC 3492
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CCTATCAAGGCTTTTGTACCAAAGTTGTCTGTGATGTCTTTTAACTCATC 100
Wildtype 3493 AACAGTTATAGAAGGAACAGCAATGGGCAGGATGTTAACATTCTGCGTCT 3542
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 AACAGTTATAGAAGGAACAGCAATGGGCAGGATGTTAACATTCTGCGTCT 150
Wildtype 3543 CCCTTTGCGCACTTTCTGTTACACGCTGCCCTGCATTGTAGCCTAAAAAA 3592
||||||| ||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 CCCTTTG-GCACTTTCTGTTACACGCTGCCCTGCATTGTAGCCTAAAAAA 199
gRNA TTGCGCACTTTCTGTTACAC
Wildtype 3593 AGATTTCCAGTTAGTGAAAACCATAAATCGTACAGGTAAAAAAAAGAATT 3642
|||||||||||||||||||||||||||||.||||||||||||||||||||
MutationRev 200 AGATTTCCAGTTAGTGAAAACCATAAATCTTACAGGTAAAAAAAAGAATT 249
Wildtype 3643 AATAGAATCTCAGCGAGTAAAATCATGACACTATCT 3678
|||||||||||||.||||||||||||||||||.|||
MutationRev 250 AATAGAATCTCAGTGAGTAAAATCATGACACTGTCT 285
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