Name: Solyc06g071500
(Exon Intron)
Length: 4565
ATGGAAGAAACATTTGTTCCCCTACGTGGAATCAAGAATGATCTCAAGGGGAGATTGTTATGCTACAAGCAAGACTGGACTGGTGGTCTCCGTGCTGGTATCAGGTTAGCTGAAGTGCAACTTAATTATCAATGATTTGCACTTGGAAAAGCATTCTTTTTCAGTTAAATTGTACTATAAACAGTGTAGTGCATAAGTAG
TAGTAGTAAATTATTTTATTCTCTATACATTTTTTGGATGCATAAAAGTTGTCTATCATATGCTGTCGCAGGATCCTGGCTCCAACGACCTACATTTTCT
TTGCATCAGCAATTCCGGTTATTTCATTCGGGGAGCAACTGGAAAGAAGCACCGGTTAGATTTGATCTTTTCCACTTGTATCAGCTACCTCTTATTGATG
GTATAGTCACTGAGATGTCATTTCTGTCAAATGTTATCTCAGATGGAAGTATAACTGCAGTGCAGACTCTTGCTTCAACAGCACTTTGTGGTGTAATCCA
TTCCATTCTTGGTGGACAGCCACTCCTTATACTAGGAGTAGCTGAGCCAACTGTCTTGATGTACACTTTCATGTTCAACTTTGCGAAAGATCGGAAGGAC
TTGGGTCCGAATCTCTTTCTAGCCTGGACAGGATGGTAACTGCTGAGCACTCTCAAACTCTTCCTAGCCTCATCATCGTCCTTTCTAACATGTTTATGTG
CAGGGTTTGTGTTTGGACCGCCTTTCTGCTTTTCTTGCTAGCCGTTCTGGGGGCGTGCTCCATAATAAACAGATTTACACGTCTGGCTGGTGAATTATTC
GGCATGCTCATAGCAATGCTCTTTATGCAAGAGGCTATTAAGGTAACCAAACTTAAATATGTTACATGATCCTAGTTTTCCGTCAATCTCCTGATCAGAA
AAAACAAATTGAAAGCAATGCAGAGTGCATCAAACCAACATAATCATGAAGAACATAAAAAAAAGTCTTAGTTCTGCTGTTGTTTTTTCTCAGAAAGTAG
CAGCCGACTTACATTCACTATAATGAAAGCTTTATCTTTGTGAAGCTCTCAGCTAAATAACCAAAAAACTTATGAAATTTATGTCTTCTTCGAACTATTT
TCAGTATTTGGCGAGGTTTGGAAGACCAATTACCTTTTAGCATCAGTAAAGCCAGTTATGAGTTATGACCCATTTCTAGAGTTTTTCCACTATTGGCTGG
TTTGCACCAAAGACAGGGTTTTCCACCAATTTGTTCAATTGTCATAACATATTGGGACAATATTCTTAGAATTAGCATAATGCTTTCCTAAATTTCAATG
CTTGTCGCAAAAATGCAGGGGTTGGTGGAGGAGTTTGGCATACCTCAGAGAGAAAATCCTCGTCAGGCTGCATTATCACCTTCTTGGCGCTTTGGAAATG
GAATGTTTGCACTAGTTTTATCTTTCGGGCTTCTTCTTACCGCACTGAGGAGCCGTAAAGCTAGATCCTGGCGTTATGGAACAGGTTTATTATTCTGAAT
AACTATGTGAATATTACTGAAAACAGAATGAAATTTTATCGTCTTAAAAACATATTATCCTTATCCTTAATATCTGATATAGTTTGTAGGTAAGGCACTT
GCTAATAGTCATCGATTTAGAATGGTTATTTCCTGTTTAACTAAGAAGGTAATTTATTAATTTGCTGACACTTCAATGTTGTTCAAAGGCTGGTTTCGAG
GATTTATTGCTGACTATGGTGTCCCACTAATGGTGCTTGTTTGGACGGCTGTTTCTTACATACCAGCACGTGATGTTCCACAAGGGATACCACGGAGGCT
TTTCAGCCCCAATCCATGGTCACCTGGAGCTTACTCAAATTGGACGGTTATCAAGGTGCTTTGCTTAAAAAATCTGTAGTATTTGATCAATCTCAAGATC
ATGGATTTAAGATTTGAGATATTGTTATCCATTTGATCTTGCATATTTTAAAACTCAAGATGATGCGTTTTACAGGAAATGATGCATGTTCCTCCTCTAT
ATATTGTCGGAGCGTTTATCCCAGCCACAATGATTGCTGTCCTTTACTATTTCGATCACAGTGTTGCATCTCAACTTTCCCAACAGAAAGAGTTCAATCT
AAAGAAACCTTCCTCATATCATTACGACCTCCTTCTTTTGGGCTTCTTGGTATGCTATTTACTCTGGCTTTCTTGTTTGTTAACATCAATTTTACATTAT
AAGAGTACATTAATAACGTTCTATGTTCACTAGGTCATTATATGCGGTCTTCTTGGCATTCCTCCTTCAAATGGAGTCATTCCACAATCTCCAATGCACA
CAAAAAGCTTGGCCACTCTGAAACATCAGGTACTGAAATTATCTCTCAATATAGAAGTCTACACAGTTCTCCACAAATTGTAACCTTGAATTGGAAGTTT
ACTTTCTTGTAGAATAATGCAAGTCCAAACACCAGCTTGCTAGGCAGTAGGCACATGTGAAATCTTAATTATTCATTATGTAATCAAAACCACATGATGG
GCCCAGAATGGAAAAAGTTTTTAGTTTATCAAGTATTTGTTTTCTCATGCTTTCTTGGTAAATTTAGGATGATCAGTCTAATTTTCTTATGTCGGAGAGT
ATTCTAATTTTCGCACTTCATTTGAAACAGCTTTTACGGAATAAACTTGCCTCAACAGCGAAAAAAAACATTGATAAAAATGCAAATCTTGGCCAATTAT
ATAGGAGCATGCAGGAGGCTTACAACGAGATGCAGACTCCCCTTGTGTATCAAACTCCTTCTGGCCTGGTAAATAGTGAGTCATTTCCAAATGGATAGGG
TTAAGATAGTTAAAATGCTTATTGTTACAGTGTTCCTTTAACAGGGATTGAAAGAGCTAAAAGAGACCACAGTTCAACGGGCATCGAGTACCGGGTACAT
AGACGCACCAGTCGACGAGACAGTCTTTGATGTGGATAAGGATATTGATGAGCTCTTACCAGTAGAAGTCAAAGAACAGCGTCTAAGTAATCTCCTTCAG
GCGTTAATGGTTGGTGCTTGTGTGGCTGCTATGCCTATCTTGAAAAGGATTCCTTCTTCTGTTCTCTGGGGATACTTTGCTTTCATGGCAATAGAGAGCT
TGCCAGGAAATCAGTTTTGGGAGAGGATATTATTGCTTTTCACTGCACCAAGTAGAAGATACAAGTAAGTCACTGCCCAATGCTCCGATCATATCTCAGT
CCCTACTCTACTTCCCATCTATTTTATTCTAATATTGTTTCGTAAGTTACAAGTGTGCCTGTCAGATCACATCCCAGAGAGAGGTCTAAAGTAATTAAGA
CTGATTAGAATCTTTAAAAAAGAGATGACTTCTTAAGTGAACTAAAAACTTTCTTTTCTGAAAGACCTTAAAGAGTTAGAATCAATCCTCATTTTCACCA
TAACCTCTTACAGTTACCCACGAGGTTCTTACATTATACAAATAGAATGTAGTCGGTTTTGTCTCTGATATCGCTTCTGCTATACCTTTCCTAATCTGCA
CATTTGTCAGGGTCTTGGAAGATAATCATGCAACATTTGTCGAGACAGTACCTTTCAAGACAATTGCCTGGTTCACCTTGTTTCAGACATTTTATTTGCT
CCTTTGTTTCGGCATCACATGGATACCAATAGCTGGTGTTCTTTTCCCTTTGTTGATCATGCTTCTAGTCCCAGTGCGCCAGTATTTGCTTCCCAAGTTC
TTCAAAGGAGCACATTTACAAGATCTAGACGCTGCAGAATATGAAGAAGCCCCTGCAATAGCATACAACATGTCCTATGGAGTAAGTTGTCAAAACCTTC
CTACATCTTTCAGCTTTTAAAAAGAAGTTCTTTTTTTATCCCTTCAGTCTTAAATAACGTTCACCACCAACCATTAATCAGAATATGAAATTTGGCCTAG
GGAAGTTGTGAATGAAAAAGTTTTTGATGGAAATGTATGATAGAAACTGTCCTGATATCATCTTGTTGATCCTATAATTTATGCACTTGCAGGATCAAGA
TCCTCAGGGAAGACCAGCCTGCATTGATAGTGGTGAGGTTCTTGATGATATGATCACAAGAAGCCGTGGGGAGATTAGGCGTTCATGCAGCCCAAGAGTA
ACTAGTTCAACTCCTACCTCTCTTGATGAAATCAAGTCAACACACAGCCCACAGTTATCTCAAAGGGCTTATAGTCCAAGAATCAATGTACTAAGAGGAG
AAAGAAGCCCCAGATTCAATGGCAAGGGACTTGAAATAAAACAAACTCCCAGCCCTCAGCCATCTAAACTTGGTCAAAATGATCGCGGTCCGTCTTCCAT
CTAGTATCAACAAATATTTTTTGTGAAATTAGAGTCTCTAGTTGTTCTCGAGAGGTCTTGTCTAAGTCTTAAGTATGTGATTAATCTTTTCCTAGGCATC
TGTGTTTCCTTGTTTGTGTGTCTTTACTGGTCTTTAACAATGTATACTGTTTTATTGTGTTGTGTTCTTGTATGCAAATCAAAATACTCTGGGCAGAGGT
TAGACAAACCATTATATCAATGAATAAAAGAGTGATAATTTTCTTAAGTACAGCTGTTTTTGTTA
The mutation and phenotype for selected feature: Solyc06g071500
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0138 | RG-PtoR | CTG0138-21 | CGR0138-21 | CRT0138-10 (T0 ) | wt (44.6%) / +1 bp (52% ) | Morphology : Boron-deficient phenotype; few seeds |
| CRT0138 | RG-PtoR | CTG0138-21 | CGR0138-21 | CRT0138-10-5 (T1 ) |
+1 bp/+1 bp
Is tDNA present: Yes
Mutation sequence: TGCAGGAGGCTTACAACGAGATGCAGACTCCCCTTGTGTATCAAACTCCTTCTGGCCTGGTAAATAGTGAGTCATTTCCA AATGGATAGGGTTAAGATAGTTAAAATGCTTATTGTTACAGTGTTCCTTTAACAGGGATTGAAAGAGCTAAAAGAGACCA CAGTTCAACGGGCATCGAGTACCGGGTAACATAGACGCACCAGTCGACGAGACAGTCTTTGATGTGGATAAGGATATTGA TGAGCTCTTACCAGTAGAAGTCAAAGAACAGCGTCTAAGTAATCTCCTTCAGGCGTTAATGGTTGGTGCTTGTGTGGCTG CTATGCCTATCTTGAAAAGGATTCCTTCTTCTGTTCTCTGGGGATACTTTGCTTTCATGGCAATAGAGAGCTTGCCAGGA AATCAGTTTTGGGAGAGGATATTATTGCTTTTCACTGCACCAAGTAGAAGATACAAGTAAGTCACTGCCCAATGCTCCGA TCATATCTCAGTCCCTACTCTACTTCCCATCTATTTTATTCTAATATTGTTTCGTAAGTTACAAGTGTGCCTGTCAGATC ACATCCCAGAGAGAGGTCTAAAGTAATTAAGACTGATTAGAATCTTTAAAAAAGAGATGACTTCTTAAGTGAACTAAAAA CTTTCTTTTCTGAAAGACCTTAAAGAGTTAGAATCAATCCTCATTTTCACCATAACCTCTTACAGTTACCCACGAGGTTC TTACATTATACAAATA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Boron-dificient phenotype; sterile |
| CRT0138 | RG-PtoR | CTG0138-21 | CGR0138-21 | CRT0138-21 (T0 ) | wt (89.3%) / -1 bp (3%) / +1 bp (5.1%) | |
| CRT0138 | RG-PtoR | CTG0138-21 | CGR0138-21 | CRT0138-4 (T0 ) | -31 bp (28.3%) / -29 bp (3.4%) / -4 bp (29.6%) / -2 bp (3.1%) |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
