| CRT0001 |
Moneymaker |
CTG0001-1 |
CGR0001-1 |
CRT0001-9 (T0 ) |
-12 bp (24.3%); -1 bp (18.8%); +1 bp (4.2%)
|
|
| CRT0001 |
Moneymaker |
CTG0001-1 |
CGR0001-1 |
CRT0001-9-1 (T1 ) |
-12 bp/-12 bp
Is tDNA present: No
Mutation sequence: TATGTGTGGAGGGCGGGGGTGATGTAGAATCTGTAGATTGTTCTTTTCTGTTTGGCAGCCATAAAGAGAACCAAGTTTCT
AATTATATAGTTGTGCTCTGTATATCAGGCAAGACCATTGTTCAAAGATCGGAGGAAGTTCTCACAGATGGCTGATCCAA
TGCTACAAGGCCATTATCCAGTAAGAGGCATGTACCAAGCTCTAGCAGTTGCAGCAATGTGTGTTCAGGAGCAACCAGTC
ATAGCAGACGTTGTCACGGCTTTGACTTACCTTGCTTCACAAAAGTTTGACCATGAAACCCGAGGGGGCATCCAAACTTC
ACGGCCAGGACCTGCTACTTCACCTAGAATGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 12 TCATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCT 61
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TCATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCT 50
Wildtype 62 CGGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGAC 111
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CGGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGAC 100
Wildtype 112 AACGTCTGCTATGACTGGACGCATGTTAGGTTGCTCCTGAACACACATTG 161
|||||||||||||||| ||||||||||||||||||||||
MutationRev 101 AACGTCTGCTATGACT------------GGTTGCTCCTGAACACACATTG 138
gRNA GCTATGACTGGACGCATGTT
Wildtype 162 CTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGT 211
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 139 CTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGT 188
Wildtype 212 AGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGG 261
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 189 AGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGG 238
Wildtype 262 TCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCT 311
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 239 TCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCT 288
Wildtype 312 TTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCC 361
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 289 TTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCC 338
Wildtype 362 GCCCTCCACACATA 375
||||||||||||||
MutationRev 339 GCCCTCCACACATA 352
|
|
| CRT0002 |
RG-prf3 |
CTG0001-1 |
CGR0001-1 |
CRT0002-16 (T0 ) |
-1 b[(30.0%); +1 bp (28.6%); WT (24.9%)
|
|
| CRT0002 |
RG-prf3 |
CTG0001-1 |
CGR0001-1 |
CRT0002-16-4 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: GCATTATATGTGTGGAGGGCGGGGGTGATGTAGAATCTGTAGATTGTTCTTTTCTGTTTGGCAGCCATAAAGAGAACCAA
GTTTCTAATTATATAGTTGTGCTCTGTATATCAGGCAAGACCATTGTTCAAAGATCGGAGGAAGTTCTCACAGATGGCTG
ATCCAATGCTACAAGGCCATTATCCAGTAAGAGGCATGTACCAAGCTCTAGCAGTTGCAGCAATGTGTGTTCAGGAGCAA
CCTAACAATGCGTCCAGTCATAGCAGACGTTGTCACGGCTTTGACTTACCTTGCTTCACAAAAGTTTGACCATGAAACCC
GAGGGGGCATCCAAACTTCACGGCCAGGACCTGCTACTTCACCTAGAATG
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 13 CATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCTC 62
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 CATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCTC 50
Wildtype 63 GGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGACA 112
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGACA 100
Wildtype 113 ACGTCTGCTATGACTGGACGCA-TGTTAGGTTGCTCCTGAACACACATTG 161
|||||||||||||||||||||| |||||||||||||||||||||||||||
MutationRev 101 ACGTCTGCTATGACTGGACGCATTGTTAGGTTGCTCCTGAACACACATTG 150
gRNA GCTATGACTGGACGCA-TGTT
Wildtype 162 CTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGT 211
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 CTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGT 200
Wildtype 212 AGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGG 261
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 AGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGG 250
Wildtype 262 TCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCT 311
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCT 300
Wildtype 312 TTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCC 361
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 TTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCC 350
Wildtype 362 GCCCTCCACACATATAATGC 381
||||||||||||||||||||
MutationRev 351 GCCCTCCACACATATAATGC 370
|
Morphology : Stunted growth; Germination <30%(Phenotype Image )
|
| CRT0002 |
RG-prf3 |
CTG0001-1 |
CGR0001-1 |
CRT0002-23 (T0 ) |
-1 bp (60.8%); WT (31.1%)
|
|
| CRT0002 |
RG-prf3 |
CTG0001-1 |
CGR0001-1 |
CRT0002-23-4 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: GGGCATTATATGTGTGGAGGGCGGGGGTGATGTAGAATCTGTAGATTGTTCTTTTCTGTTTGGCAGCCATAAAGAGAACC
AAGTTTCTAATTATATAGTTGTGCTCTGTATATCAGGCAAGACCATTGTTCAAAGATCGGAGGAAGTTCTCACAGATGGC
TGATCCAATGCTACAAGGCCATTATCCAGTAAGAGGCATGTACCAAGCTCTAGCAGTTGCAGCAATGTGTGTTCAGGAGC
AACCTAACTGCGTCCAGTCATAGCAGACGTTGTCACGGCTTTGACTTACCTTGCTTCACAAAAGTTTGACCATGAAACCC
GAGGGGGCATCCAAACTTCACGGCCAGGACCTGCTACTTCACCTA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 18 TAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCTCGGGTT 67
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCTCGGGTT 50
Wildtype 68 TCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGACAACGTC 117
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 TCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGACAACGTC 100
Wildtype 118 TGCTATGACTGGACGCATGTTAGGTTGCTCCTGAACACACATTGCTGCAA 167
||||||||||||||||| ||||||||||||||||||||||||||||||||
MutationRev 101 TGCTATGACTGGACGCA-GTTAGGTTGCTCCTGAACACACATTGCTGCAA 149
gRNA GCTATGACTGGACGCATGTT
Wildtype 168 CTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGTAGCATT 217
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 150 CTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGTAGCATT 199
Wildtype 218 GGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGGTCTTGC 267
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 200 GGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGGTCTTGC 249
Wildtype 268 CTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCTTTATGG 317
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 250 CTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCTTTATGG 299
Wildtype 318 CTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCCGCCCTC 367
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 300 CTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCCGCCCTC 349
Wildtype 368 CACACATATAATGCCC 383
||||||||||||||||
MutationRev 350 CACACATATAATGCCC 365
|
Morphology : Stunted growth; Germination >90%(Phenotype Image )
|
| CRT0002 |
RG-prf3 |
CTG0001-1 |
CGR0001-1 |
CRT0002-32 (T0 ) |
-1 bp (48%); -3 bp (48%)
|
|
| CRT0002 |
RG-prf3 |
CTG0001-1 |
CGR0001-1 |
CRT0002-32-3 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: CATTATATGTGTGGAGGGCGGGGGTGATGTAGAATCTGTAGATTGTTCTTTTCTGTTTGGCAGCCATAAAGAGAACCAAG
TTTCTAATTATATAGTTGTGCTCTGTATATCAGGCAAGACCATTGTTCAAAGATCGGAGGAAGTTCTCACAGATGGCTGA
TCCAATGCTACAAGGCCATTATCCAGTAAGAGGCATGTACCAAGCTCTAGCAGTTGCAGCAATGTGTGTTCAGGAGCAAC
CTAACTGCGTCCAGTCATAGCAGACGTTGTCACGGCTTTGACTTACCTTGCTTCACAAAAGTTTGACCATGAAACCCGAG
GGGGCATCCAAACTTCACGGCCAGGACCTGCTACTTCACCTAGAATGAA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 11 TTCATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TTCATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCC 50
Wildtype 61 TCGGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGA 110
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 TCGGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGA 100
Wildtype 111 CAACGTCTGCTATGACTGGACGCATGTTAGGTTGCTCCTGAACACACATT 160
|||||||||||||||||||||||| |||||||||||||||||||||||||
MutationRev 101 CAACGTCTGCTATGACTGGACGCA-GTTAGGTTGCTCCTGAACACACATT 149
gRNA GCTATGACTGGACGCATGTT
Wildtype 161 GCTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTG 210
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 150 GCTGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTG 199
Wildtype 211 TAGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATG 260
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 200 TAGCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATG 249
Wildtype 261 GTCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTC 310
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 250 GTCTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTC 299
Wildtype 311 TTTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 300 TTTATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCC 349
Wildtype 361 CGCCCTCCACACATATAATG 380
||||||||||||||||||||
MutationRev 350 CGCCCTCCACACATATAATG 369
|
|
| CRT0002 |
RG-prf3 |
CTG0001-1 |
CGR0001-1 |
CRT0002-32-5 (T1 ) |
-3 bp/-3 bp
Is tDNA present: No
Mutation sequence: GGGCATTATATGTGTGGAGGGCGGGGGTGATGTAGAATCTGTAGATTGTTCTTTTCTGTTTGGCAGCCATAAAGAGAACC
AAGTTTCTAATTATATAGTTGTGCTCTGTATATCAGGCAAGACCATTGTTCAAAGATCGGAGGAAGTTCTCACAGATGGC
TGATCCAATGCTACAAGGCCATTATCCAGTAAGAGGCATGTACCAAGCTCTAGCAGTTGCAGCAATGTGTGTTCAGGAGC
AACCTATGCGTCCAGTCATAGCAGACGTTGTCACGGCTTTGACTTACCTTGCTTCACAAAAGTTTGACCATGAAACCCGA
GGGGGCATCCAAACTTCACGGCCAGGACCTGCTACTTCACCTAGAATG
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 13 CATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCTC 62
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 CATTCTAGGTGAAGTAGCAGGTCCTGGCCGTGAAGTTTGGATGCCCCCTC 50
Wildtype 63 GGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGACA 112
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 GGGTTTCATGGTCAAACTTTTGTGAAGCAAGGTAAGTCAAAGCCGTGACA 100
Wildtype 113 ACGTCTGCTATGACTGGACGCATGTTAGGTTGCTCCTGAACACACATTGC 162
|||||||||||||||||||||| |||||||||||||||||||||||||
MutationRev 101 ACGTCTGCTATGACTGGACGCA---TAGGTTGCTCCTGAACACACATTGC 147
gRNA GCTATGACTGGACGCATGTT
Wildtype 163 TGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGTA 212
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 148 TGCAACTGCTAGAGCTTGGTACATGCCTCTTACTGGATAATGGCCTTGTA 197
Wildtype 213 GCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGGT 262
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 198 GCATTGGATCAGCCATCTGTGAGAACTTCCTCCGATCTTTGAACAATGGT 247
Wildtype 263 CTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCTT 312
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 248 CTTGCCTGATATACAGAGCACAACTATATAATTAGAAACTTGGTTCTCTT 297
Wildtype 313 TATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCCG 362
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 298 TATGGCTGCCAAACAGAAAAGAACAATCTACAGATTCTACATCACCCCCG 347
Wildtype 363 CCCTCCACACATATAATGCCC 383
|||||||||||||||||||||
MutationRev 348 CCCTCCACACATATAATGCCC 368
|
|
| CRT0073 |
RG-PtoR |
CTG0073-2 |
CGR0073-2 |
CRT0073-1 (T0 ) |
-7 bp (13.1%); WT (85%)
|
|
| CRT0073 |
RG-PtoR |
CTG0073-2 |
CGR0073-2 |
CRT0073-1-6 (T1 ) |
azygous
Is tDNA present: Yes
Mutation sequence: TTTAATGGTGCAGGTCGTCGCCATCAAGCAACTAGATCGTAATGGATTGCAGGGCAACAGGGAATTTCTTGTCGAAGTGC
TGATGTTAAGTCTGTTACACCATCCTAACCTTGTAAATCTAATTGGCTATTGTGCTGATGGAGATCAGAGACTTCTGGTC
TACGAATATATGCCATTAGGATCATTGGAGGACCATCTTCATGGTAATATTTCTTAGGTAGAATTTCCTTTTCATTTTAT
TTGAAGTCGGATTAAATTTGGAATTGAAGTATTGCTCTGCAGATCCTACGCCAGGTAAGGAACGGCTAGATTGGAACACA
AGAATGAAAATTGCTGCCGGAGCAGCAAA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1348 TTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCCGTTC 1397
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCCGTTC 50
Wildtype 1398 CTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTTAATC 1447
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTTAATC 100
Wildtype 1448 CGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATG 1497
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 CGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATG 150
Wildtype 1498 AAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGAAGTC 1547
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 AAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGAAGTC 200
gRNA AATGGCATATATTCGTAGAC
Wildtype 1548 TCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGGATGG 1597
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGGATGG 250
Wildtype 1598 TGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGCCCTG 1647
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGCCCTG 300
Wildtype 1648 CAATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATTAAA 1696
|||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 CAATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATTAAA 349
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-1 (T0 ) |
-4 bp (35%); WT (62.5%)
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-1-12 (T1 ) |
-4 bp/-4 bp
Is tDNA present: No
Mutation sequence: ACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAGGG
GGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCCGTTC
CTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCAAATAAAATGAAAAGGAAATTCT
ACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCC
ATCAGCACAATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCC
TGTTGCCCTGCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAAACTTCA
GCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAATGACTTT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1238 ACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTG 1287
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTG 50
Wildtype 1288 GAGCATTTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATA 1337
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 GAGCATTTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATA 100
Wildtype 1338 TTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAAT 1387
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAAT 150
Wildtype 1388 CTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCC 1437
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 CTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCC 200
Wildtype 1438 AAATTTAATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAA 1487
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 AAATTTAATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAA 250
Wildtype 1488 TATTACCATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAG 1537
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 TATTACCATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAG 300
Wildtype 1538 ACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAG 1587
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 ACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAG 350
Wildtype 1588 GTTAGGATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCC 1637
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 GTTAGGATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCC 400
Wildtype 1638 TGTTGCCCTGCAATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGC 1687
|||||||||| ||||||||||||||||||||||||||||||||||||
Mutation 401 TGTTGCCCTG----CCATTACGATCTAGTTGCTTGATGGCGACGACCTGC 446
gRNA TGCAATCCATTACGATCTA
Wildtype 1688 ACCATTAAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACT 1737
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 447 ACCATTAAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACT 496
Wildtype 1738 TAGACGTGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAAT 1787
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 497 TAGACGTGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAAT 546
Wildtype 1788 GTAAATGACTTT 1799
||||||||||||
Mutation 547 GTAAATGACTTT 558
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-1-13 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: GGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCATTTCAAATC
ACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGT
TCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCAAATAAA
ATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCA
GAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATCAGCACT
TCGACAAGAAATTCCCTGTTGCCCTGCAATCCATTACGATCTAGTTGCTTGATG
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1222 GGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTC 1271
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 GGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTC 50
Wildtype 1272 ACCAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAGGGGGAC 1321
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ACCAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAGGGGGAC 100
Wildtype 1322 TTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTC 1371
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTC 150
Wildtype 1372 ATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGA 1421
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 ATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGA 200
Wildtype 1422 GCAATACTTCAATTCCAAATTTAATCCGACTTCAAATAAAATGAAAAGGA 1471
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 GCAATACTTCAATTCCAAATTTAATCCGACTTCAAATAAAATGAAAAGGA 250
Wildtype 1472 AATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGATCCTAA 1521
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 AATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGATCCTAA 300
Wildtype 1522 TGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCACAATAGC 1571
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 TGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCACAATAGC 350
Wildtype 1572 CAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATCAGCACT 1621
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 CAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATCAGCACT 400
Wildtype 1622 TCGACAAGAAATTCCCTGTTGCCCTGCAATCCATTACGATCTAGTTGCTT 1671
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 TCGACAAGAAATTCCCTGTTGCCCTGCAATCCATTACGATCTAGTTGCTT 450
gRNA TGCAATCCATTACGATCTA
Wildtype 1672 GATG 1675
||||
Mutation 451 GATG 454
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-2 (T0 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCAC
CAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCT
GCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAA
TTCCAAATTTAATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAAT
GATCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG
ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGCCCTGCATCCATTACGATCTAGTTGCTTGAT
GGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAA
CCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAATGACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCT
CACATATATGTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCC
ATGAAATGTACATCACCACTTGTTAGAAAGAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGAT
GCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAAAAAC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1194 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA 1243
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA 50
Wildtype 1244 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT 1293
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT 100
Wildtype 1294 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA 1343
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA 150
Wildtype 1344 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC 1393
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC 200
Wildtype 1394 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT 1443
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT 250
Wildtype 1444 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC 1493
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC 300
Wildtype 1494 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA 1543
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA 350
Wildtype 1544 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG 1593
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG 400
Wildtype 1594 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC 1643
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC 450
Wildtype 1644 CCTGCAATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT 1693
||||| ||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CCTGC-ATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT 499
gRNA TGCAATCCATTACGATCTA
Wildtype 1694 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG 1743
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 500 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG 549
Wildtype 1744 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT 1793
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 550 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT 599
Wildtype 1794 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT 1843
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 600 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT 649
Wildtype 1844 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC 1893
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 650 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC 699
Wildtype 1894 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA 1943
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 700 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA 749
Wildtype 1944 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA 1993
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 750 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA 799
Wildtype 1994 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA 2043
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 800 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA 849
Wildtype 2044 AAAC 2047
||||
Mutation 850 AAAC 853
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-2-1 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCAC
CAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCT
GCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAA
TTCCAAATTTAATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAAT
GATCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG
ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGCCCTGCATCCATTACGATCTAGTTGCTTGAT
GGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAA
CCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAATGACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCT
CACATATATGTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCC
ATGAAATGTACATCACCACTTGTTAGAAAGAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGAT
GCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAAAAAC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1194 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA 1243
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA 50
Wildtype 1244 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT 1293
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT 100
Wildtype 1294 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA 1343
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA 150
Wildtype 1344 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC 1393
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC 200
Wildtype 1394 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT 1443
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT 250
Wildtype 1444 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC 1493
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC 300
Wildtype 1494 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA 1543
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA 350
Wildtype 1544 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG 1593
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG 400
Wildtype 1594 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC 1643
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC 450
Wildtype 1644 CCTGCAATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT 1693
||||| ||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CCTGC-ATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT 499
gRNA TGCAATCCATTACGATCTA
Wildtype 1694 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG 1743
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 500 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG 549
Wildtype 1744 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT 1793
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 550 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT 599
Wildtype 1794 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT 1843
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 600 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT 649
Wildtype 1844 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC 1893
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 650 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC 699
Wildtype 1894 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA 1943
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 700 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA 749
Wildtype 1944 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA 1993
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 750 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA 799
Wildtype 1994 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA 2043
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 800 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA 849
Wildtype 2044 AAAC 2047
||||
Mutation 850 AAAC 853
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-2-3 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCAC
CAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCT
GCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAA
TTCCAAATTTAATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAAT
GATCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG
ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGCCCTGCATCCATTACGATCTAGTTGCTTGAT
GGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAA
CCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAATGACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCT
CACATATATGTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCC
ATGAAATGTACATCACCACTTGTTAGAAAGAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGAT
GCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAAAAAC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1194 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA 1243
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA 50
Wildtype 1244 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT 1293
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT 100
Wildtype 1294 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA 1343
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA 150
Wildtype 1344 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC 1393
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC 200
Wildtype 1394 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT 1443
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT 250
Wildtype 1444 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC 1493
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC 300
Wildtype 1494 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA 1543
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA 350
Wildtype 1544 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG 1593
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG 400
Wildtype 1594 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC 1643
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC 450
Wildtype 1644 CCTGCAATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT 1693
||||| ||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CCTGC-ATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT 499
gRNA TGCAATCCATTACGATCTA
Wildtype 1694 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG 1743
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 500 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG 549
Wildtype 1744 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT 1793
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 550 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT 599
Wildtype 1794 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT 1843
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 600 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT 649
Wildtype 1844 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC 1893
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 650 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC 699
Wildtype 1894 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA 1943
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 700 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA 749
Wildtype 1944 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA 1993
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 750 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA 799
Wildtype 1994 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA 2043
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 800 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA 849
Wildtype 2044 AAAC 2047
||||
Mutation 850 AAAC 853
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-3 (T0 ) |
-1 bp (36.2%); WT (62.4%)
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-3-8 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCAC
CAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCT
GCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAA
TTCCAAATTTAATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAAT
GATCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG
ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGCCCTGCATCCATTACGATCTAGTTGCTTGAT
GGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAA
CCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAATGACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCT
CACATATATGTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCC
ATGAAATGTACATCACCACTTGTTAGAAAGAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGAT
GCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAAAAAC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1194 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA 1243
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 TGTAGAAACATGGGTGTTATCCCCTACAGGCCCCAATTTGGCCAACCCGA 50
Wildtype 1244 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT 1293
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCAT 100
Wildtype 1294 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA 1343
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TTCAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAA 150
Wildtype 1344 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC 1393
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TCCTTTTGCTGCTCCGGCAGCAATTTTCATTCTTGTGTTCCAATCTAGCC 200
Wildtype 1394 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT 1443
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 GTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTT 250
Wildtype 1444 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC 1493
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 AATCCGACTTCAAATAAAATGAAAAGGAAATTCTACCTAAGAAATATTAC 300
Wildtype 1494 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA 1543
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGTAGACCAGA 350
Wildtype 1544 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG 1593
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 AGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGG 400
Wildtype 1594 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC 1643
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 ATGGTGTAACAGACTTAACATCAGCACTTCGACAAGAAATTCCCTGTTGC 450
Wildtype 1644 CCTGCAATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT 1693
||||| ||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CCTGC-ATCCATTACGATCTAGTTGCTTGATGGCGACGACCTGCACCATT 499
gRNA TGCAATCCATTACGATCTA
Wildtype 1694 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG 1743
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 500 AAAAGTCAACAAAAGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACG 549
Wildtype 1744 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT 1793
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 550 TGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAATGTAAAT 599
Wildtype 1794 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT 1843
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 600 GACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATAT 649
Wildtype 1844 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC 1893
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 650 GTGAGCAAAGGGAATTGAGCCCAATTAGCTCAACGAATATGATAAAAACC 699
Wildtype 1894 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA 1943
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 700 CTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAA 749
Wildtype 1944 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA 1993
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 750 GAAGAAAAGCATTTTCCTTATTTGAGTAAGGCCTAGTCTCACTTTGCAGA 799
Wildtype 1994 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA 2043
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 800 TGCTAAAGAACAAAAGTGAAATTTACGTTTCAGATTTCTCATGTAACTAA 849
Wildtype 2044 AAAC 2047
||||
Mutation 850 AAAC 853
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| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-4 (T0 ) |
-1 bp (12%); +1 bp (12.6%); WT (74%)
|
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| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-4-7 (T1 ) |
-1 bp (71.5%); WT 12%; +1 bp 14%
|
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| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-5 (T0 ) |
-11 bp/-11 bp
Is tDNA present: Yes
Mutation sequence: CCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCATTT
CAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTCATTC
TTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCA
AATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGT
AGACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATC
AGCACTTCGACAAGAAATTCCCTGTTGCCCACGATCTAGTTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAA
AGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAA
TGTAAATGACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATATGTGAGCAAAGGGAATTGAGCCCA
ATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAAGAA
GAAAAGCATTTTCCTTATTTGAGTAAGG
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1216 CCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATA 1265
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 CCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATA 50
Wildtype 1266 ACCCTCACCAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAG 1315
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ACCCTCACCAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAG 100
Wildtype 1316 GGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCA 1365
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 GGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCA 150
Wildtype 1366 ATTTTCATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATC 1415
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 ATTTTCATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATC 200
Wildtype 1416 TGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCAAATAAAATGA 1465
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCAAATAAAATGA 250
Wildtype 1466 AAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGA 1515
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 AAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGA 300
Wildtype 1516 TCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCAC 1565
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 TCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCAC 350
Wildtype 1566 AATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATC 1615
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 AATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATC 400
Wildtype 1616 AGCACTTCGACAAGAAATTCCCTGTTGCCCTGCAATCCATTACGATCTAG 1665
|||||||||||||||||||||||||||||| |||||||||
Mutation 401 AGCACTTCGACAAGAAATTCCCTGTTGCCC-----------ACGATCTAG 439
gRNA TGCAATCCATTACGATCTA
Wildtype 1666 TTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAA 1715
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 440 TTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAA 489
Wildtype 1716 ACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCAT 1765
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 490 ACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCAT 539
Wildtype 1766 GGTGGCAACACTAGAAGAAAATGTAAATGACTTTGTCAGTTATACCTTTG 1815
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 540 GGTGGCAACACTAGAAGAAAATGTAAATGACTTTGTCAGTTATACCTTTG 589
Wildtype 1816 CCTATTTCAACTCGAACCTCACATATATGTGAGCAAAGGGAATTGAGCCC 1865
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 590 CCTATTTCAACTCGAACCTCACATATATGTGAGCAAAGGGAATTGAGCCC 639
Wildtype 1866 AATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCCA 1915
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 640 AATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCCA 689
Wildtype 1916 TGAAATGTACATCACCACTTGTTAGAAAGAAGAAAAGCATTTTCCTTATT 1965
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 690 TGAAATGTACATCACCACTTGTTAGAAAGAAGAAAAGCATTTTCCTTATT 739
Wildtype 1966 TGAGTAAGG 1974
|||||||||
Mutation 740 TGAGTAAGG 748
|
|
| CRT0074 |
RG-PtoR |
CTG0074-1 |
CGR0074-1 |
CRT0074-5-1 (T1 ) |
-11 bp/-11 bp
Is tDNA present: Yes
Mutation sequence: CCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATAACCCTCACCAAGCAAAATGTTGGAGCATTT
CAAATCACGATATATTACAGGGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCAATTTTCATTC
TTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATCTGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCA
AATAAAATGAAAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGATCCTAATGGCATATATTCGT
AGACCAGAAGTCTCTGATCTCCATCAGCACAATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATC
AGCACTTCGACAAGAAATTCCCTGTTGCCCACGATCTAGTTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAA
AGGTGTTAAACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCATGGTGGCAACACTAGAAGAAAA
TGTAAATGACTTTGTCAGTTATACCTTTGCCTATTTCAACTCGAACCTCACATATATGTGAGCAAAGGGAATTGAGCCCA
ATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCCATGAAATGTACATCACCACTTGTTAGAAAGAA
GAAAAGCATTTTCCTTATTTGAGTAAGG
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1216 CCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATA 1265
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 CCTACAGGCCCCAATTTGGCCAACCCGAAGTCAGAGAGCTTCGCATGATA 50
Wildtype 1266 ACCCTCACCAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAG 1315
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ACCCTCACCAAGCAAAATGTTGGAGCATTTCAAATCACGATATATTACAG 100
Wildtype 1316 GGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCA 1365
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 GGGGACTTGCACTGTGTAAATATTCCAATCCTTTTGCTGCTCCGGCAGCA 150
Wildtype 1366 ATTTTCATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATC 1415
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 ATTTTCATTCTTGTGTTCCAATCTAGCCGTTCCTTACCTGGCGTAGGATC 200
Wildtype 1416 TGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCAAATAAAATGA 1465
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TGCAGAGCAATACTTCAATTCCAAATTTAATCCGACTTCAAATAAAATGA 250
Wildtype 1466 AAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGA 1515
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 AAAGGAAATTCTACCTAAGAAATATTACCATGAAGATGGTCCTCCAATGA 300
Wildtype 1516 TCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCAC 1565
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 TCCTAATGGCATATATTCGTAGACCAGAAGTCTCTGATCTCCATCAGCAC 350
Wildtype 1566 AATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATC 1615
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 AATAGCCAATTAGATTTACAAGGTTAGGATGGTGTAACAGACTTAACATC 400
Wildtype 1616 AGCACTTCGACAAGAAATTCCCTGTTGCCCTGCAATCCATTACGATCTAG 1665
|||||||||||||||||||||||||||||| |||||||||
Mutation 401 AGCACTTCGACAAGAAATTCCCTGTTGCCC-----------ACGATCTAG 439
gRNA TGCAATCCATTACGATCTA
Wildtype 1666 TTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAA 1715
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 440 TTGCTTGATGGCGACGACCTGCACCATTAAAAGTCAACAAAAGGTGTTAA 489
Wildtype 1716 ACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCAT 1765
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 490 ACTTCAGCTCCAGCTAACAACTTAGACGTGAGAAGCAAACCATCAAGCAT 539
Wildtype 1766 GGTGGCAACACTAGAAGAAAATGTAAATGACTTTGTCAGTTATACCTTTG 1815
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 540 GGTGGCAACACTAGAAGAAAATGTAAATGACTTTGTCAGTTATACCTTTG 589
Wildtype 1816 CCTATTTCAACTCGAACCTCACATATATGTGAGCAAAGGGAATTGAGCCC 1865
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 590 CCTATTTCAACTCGAACCTCACATATATGTGAGCAAAGGGAATTGAGCCC 639
Wildtype 1866 AATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCCA 1915
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 640 AATTAGCTCAACGAATATGATAAAAACCCTAATAGAAACCAAAGTATCCA 689
Wildtype 1916 TGAAATGTACATCACCACTTGTTAGAAAGAAGAAAAGCATTTTCCTTATT 1965
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 690 TGAAATGTACATCACCACTTGTTAGAAAGAAGAAAAGCATTTTCCTTATT 739
Wildtype 1966 TGAGTAAGG 1974
|||||||||
Mutation 740 TGAGTAAGG 748
|
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