Is tDNA present:

Mutation sequence:

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
ACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACA
TTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTTC
CAAAGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT
GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTC
TTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAG
ACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGAT
TTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCA
GAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA
GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTT
TCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGA
GAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATC
TAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
gRNA                       CCAGACAAAATAAGACAACC

Wildtype         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250

Wildtype         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300

Wildtype         301 GAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGAG    350
                     ||||||||||||||||||      ||||||||||||||||||||||||||
Mutation         301 GAACAACCAAGAAAATCT------AAGAGGCGCAGCATCCACAGGAGGAG    344

Wildtype         351 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         345 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    394

Wildtype         401 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         395 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    444

Wildtype         451 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         445 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    494

Wildtype         501 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         495 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    544

Wildtype         551 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         545 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    594

Wildtype         601 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         595 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    644

Wildtype         651 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         645 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    694

Wildtype         701 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         695 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    744

Wildtype         751 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         745 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    794

Wildtype         801 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         795 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    844

Wildtype         851 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         845 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    894

Wildtype         901 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         895 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    944

Wildtype         951 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         945 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG    994

Wildtype        1001 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         995 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1044

Wildtype        1051 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     ||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1045 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1092

Is tDNA present:

Mutation sequence:

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
ACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACA
TTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTTC
CAAAGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT
GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTC
TTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAG
ACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGAT
TTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCA
GAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA
GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTT
TCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGA
GAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATC
TAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
gRNA                       CCAGACAAAATAAGACAACC

Wildtype         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250

Wildtype         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300

Wildtype         301 GAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGAG    350
                     ||||||||||||||||||      ||||||||||||||||||||||||||
Mutation         301 GAACAACCAAGAAAATCT------AAGAGGCGCAGCATCCACAGGAGGAG    344

Wildtype         351 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         345 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    394

Wildtype         401 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         395 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    444

Wildtype         451 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         445 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    494

Wildtype         501 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         495 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    544

Wildtype         551 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         545 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    594

Wildtype         601 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         595 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    644

Wildtype         651 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         645 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    694

Wildtype         701 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         695 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    744

Wildtype         751 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         745 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    794

Wildtype         801 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         795 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    844

Wildtype         851 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         845 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    894

Wildtype         901 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         895 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    944

Wildtype         951 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         945 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG    994

Wildtype        1001 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         995 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1044

Wildtype        1051 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     ||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1045 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1092

Is tDNA present:

Mutation sequence:

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
ACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACA
TTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTTC
CAAAGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT
GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTC
TTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAG
ACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGAT
TTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCA
GAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA
GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTT
TCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGA
GAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATC
TAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200

Wildtype         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250

Wildtype         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300

Wildtype         301 GAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGAG    350
                     ||||||||||||||||||      ||||||||||||||||||||||||||
Mutation         301 GAACAACCAAGAAAATCT------AAGAGGCGCAGCATCCACAGGAGGAG    344
gRNA                        CAAGAAAATCTTCCAAGAAG

Wildtype         351 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         345 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    394

Wildtype         401 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         395 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    444

Wildtype         451 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         445 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    494

Wildtype         501 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         495 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    544

Wildtype         551 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         545 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    594

Wildtype         601 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         595 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    644

Wildtype         651 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         645 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    694

Wildtype         701 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         695 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    744

Wildtype         751 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         745 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    794

Wildtype         801 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         795 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    844

Wildtype         851 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         845 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    894

Wildtype         901 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         895 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    944

Wildtype         951 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         945 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG    994

Wildtype        1001 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         995 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1044

Wildtype        1051 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     ||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1045 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1092

Is tDNA present:

Mutation sequence:

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
ACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACA
TTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTTC
CAAAGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT
GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTC
TTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAG
ACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGAT
TTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCA
GAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA
GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTT
TCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGA
GAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATC
TAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200

Wildtype         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250

Wildtype         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300

Wildtype         301 GAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGAG    350
                     ||||||||||||||||||      ||||||||||||||||||||||||||
Mutation         301 GAACAACCAAGAAAATCT------AAGAGGCGCAGCATCCACAGGAGGAG    344
gRNA                        CAAGAAAATCTTCCAAGAAG

Wildtype         351 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         345 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    394

Wildtype         401 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         395 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    444

Wildtype         451 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         445 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    494

Wildtype         501 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         495 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    544

Wildtype         551 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         545 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    594

Wildtype         601 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         595 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    644

Wildtype         651 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         645 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    694

Wildtype         701 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         695 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    744

Wildtype         751 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         745 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    794

Wildtype         801 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         795 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    844

Wildtype         851 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         845 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    894

Wildtype         901 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         895 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    944

Wildtype         951 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         945 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG    994

Wildtype        1001 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         995 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1044

Wildtype        1051 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     ||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1045 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1092