Is tDNA present:

Mutation sequence:

TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTCTCAAAGCCCAAGAACAATATTTCCTGACCA
GATTCCAAGTTCAGCTTTTTGGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGCTCCAGTCCAG
GTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGATATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGT
CCAGAGGTTCACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTGCATAGCGGAAGTGTCTCTCCA
TTGCATCCGAGGGCTGGTGGGATGGCACCTGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACAG
ATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCGAACAACTCCGCCCCAACAAGTCCTATATCTC
GTAGTCCTGGTAGAGCAGAGAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2542 TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTC   2591
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTC     50

Wildtype        2592 TCAAAGCCCAAGAACAATATTTCCTGACCAGATTCCAAGTTCAGCTTTTT   2641
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TCAAAGCCCAAGAACAATATTTCCTGACCAGATTCCAAGTTCAGCTTTTT    100

Wildtype        2642 GGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGC   2691
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 GGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGC    150

Wildtype        2692 TCCAGTCCAGGTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGA   2741
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCCAGTCCAGGTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGA    200

Wildtype        2742 TATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGTCCAGAGTGTT   2791
                     |||||||||||||||||||||||||||||||||||||||||||||| |||
Mutation         201 TATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGTCCAGAG-GTT    249
gRNA                                                            GAGTGTT

Wildtype        2792 CACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTG   2841
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 CACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTG    299
gRNA                 GAGTGTTCACCAATTCCTAG

Wildtype        2842 CATAGCGGAAGTGTCTCTCCATTGCATCCGAGGGCTGGTGGGATGGCACC   2891
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 CATAGCGGAAGTGTCTCTCCATTGCATCCGAGGGCTGGTGGGATGGCACC    349

Wildtype        2892 TGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACA   2941
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         350 TGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACA    399

Wildtype        2942 GATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCG   2991
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         400 GATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCG    449

Wildtype        2992 AACAACTCCGCCCCAACAAGTCCTATATCTCGTAGTCCTGGTAGAGCAGA   3041
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         450 AACAACTCCGCCCCAACAAGTCCTATATCTCGTAGTCCTGGTAGAGCAGA    499

Wildtype        3042 GAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA   3080
                     |||||||||||||||||||||||||||||||||||||||
Mutation         500 GAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA    538