Plant Crispr Database

Plant Information

Name: CRT0003-1-49

Seed stock: 5044
Seed availability: No

Relationship:
Parent: CRT0003-1
Progeny: CRT0003-1-49-121

Transformation: CRT0003
transformation CRT0003 to RG-prf3

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0003-1-49 (T1 )	CRT0003	CTG0003-1	CGR0003-1	gene : Solyc11g072910 (Mal1)	WT(-45%); -4 bp (-35%)
CRT0003-1-49 (T1 )	CRT0003	CTG0003-2	CGR0003-2	gene : Solyc06g005230 (Mal2)	-200 bp/-200 bp Is tDNA present: Yes Mutation sequence: TTGTCATTAAATAGTTTATTTTTTGTACTGTTGTTGGGTTTTTACTCAAACTTCTAGTTATAAATTCCCTATTAGTAAAC TTGGTAGGCATTGGATCCGTCTTCATTTTTCCCCCTTTGTGTACCAATCTTATGATATGAGTTTAGCAACTTTCTCTGTT TCAACTCAACAAAATGTGCTCCTTGGAAATTTTAGTCCTAAAAAAGATGTATCTGTTAAGGAGTTTTCTGTTAACGTAAC GACGGGTGATCTTGTCATCACTTTTTCTCCTTTGAGTGATTCATTTGCTTATGTAAATGCTTTGGAAGTTGTCTCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 370 CCACTTCATCGCCCATTCAGCTAAATTAACCATCTCCCTTGGAAG-AGAT 418 \|.\|\|\|\|\| \|\| \|\|.\|\|\|.\|.\|.\| \|\|\| \|.\|\| MutationRev 6 CAACTTC----------CA--------AAGCATTTACAT---AAGCAAAT 34 Wildtype 419 GGATCGATTACAGGCCTAGCACAAAG-------AACTTCA---------- 451 \|.\|\|\|..\|.\|.\|\|\| \|\| \|.\|\|\|\| \|\|..\|\|\| MutationRev 35 GAATCACTCAAAGG---AG-AAAAAGTGATGACAAGATCACCCGTCGTTA 80 Wildtype 452 ----AACAGAACAACACCAAAAGAGTAAACATCACTTTTCTCAGTCAATT 497 \|\|\|\|\|\|\| \|\|\|.\|\|..\|\|.\|\| \|.\|\|\|\|\|..\|\|\|\|...\| MutationRev 81 CGTTAACAGAA-AACTCCTTAACAG-ATACATCTTTTTTAGGA------- 121 Wildtype 498 GCTGCCTCCTGAAATATTCGGGATCTAGGTACCCGAAGCTCCCTTTCACT 547 \|\|.\|\|\|\| \|\|\| \|.\|\|\| \|\|\|.\|..\|\|\| MutationRev 122 --------CTAAAAT-TTC-----CAAGG-------AGCACATTTT---- 146 Wildtype 548 GCCGTGCTAACATGAGTTTGATCGAGCTCGGGTCCTGCCTTAGAAAGTCC 597 \|\| \|\|\|\| \|\|\|\|...\|\| \|\|\|\|\|\| MutationRev 147 ---GT-------TGAG-TTGAAACAG---------------AGAAAG--- 167 Wildtype 598 GAAATCAGCTACTTTTGCCA---TCATATTCTCATCAAGCAATATGTTTG 644 \|\|\|\|.\| \|\|\|\|\| \|\|\|\|.\| MutationRev 168 --------------TTGCTAAACTCATA---TCATAA------------- 187 Wildtype 645 CTGATTTG-ACATCACGATGTATAACTGCTTTGGCGTAGCTAGTATGAAG 693 \|\|\|\|.\| \|\|\|..\|.\|..\|.\|.\|\| \|\|\|\| MutationRev 188 --GATTGGTACACAAAGGGGGAAAA-------------------ATGA-- 214 Wildtype 694 GTAGTGTAGACCTCTGGCTGCCCCAATACATATCTCCAGCCTCTGTTTCC 743 \|\|\|\| \|\|.\| \|\|\|\|\|.\|.\|\| \|\|\| .\|\|\|\|.\| MutationRev 215 -------AGAC----GGAT---CCAATGCCTA---CCA-----AGTTTAC 242 Wildtype 744 AGCTCATACTAGGCA---TATCCGAACCATACAGATGGCTCTTAAGGGTC 790 \|\|.\|\|\|\|.\| \|\|\| \|\|\|.\|.\| MutationRev 243 ------TAATAGGGAATTTAT---AACTAGA------------------- 264 Wildtype 791 CCATTTTCCATGTACTCGTACACGAGAATCATCTCATTCTTTTCATCACA 840 \|.\|\|\| \|\|\|.\|..\|.\|.\|\| \|..\|\|\| MutationRev 265 --AGTTT----------------GAGTAAAAACCCA--------ACAACA 288 Wildtype 841 GTATCCAATCAATGAAACAA---GATGACGA 868 \|\|\| \|\|\|..\|\|\| \|\|\|\|.\| .\|\|\|\|\|.\| MutationRev 289 GTA--CAAAAAAT-AAACTATTTAATGACAA 316

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0003-1-49 (T1 )

CRT0003

CTG0003-1

CGR0003-1

gene : Solyc11g072910 (Mal1)

WT(-45%); -4 bp (-35%)

CRT0003-1-49 (T1 )

CRT0003

CTG0003-2

CGR0003-2

gene : Solyc06g005230 (Mal2)

-200 bp/-200 bp

Is tDNA present: Yes

Mutation sequence:

TTGTCATTAAATAGTTTATTTTTTGTACTGTTGTTGGGTTTTTACTCAAACTTCTAGTTATAAATTCCCTATTAGTAAAC
TTGGTAGGCATTGGATCCGTCTTCATTTTTCCCCCTTTGTGTACCAATCTTATGATATGAGTTTAGCAACTTTCTCTGTT
TCAACTCAACAAAATGTGCTCCTTGGAAATTTTAGTCCTAAAAAAGATGTATCTGTTAAGGAGTTTTCTGTTAACGTAAC
GACGGGTGATCTTGTCATCACTTTTTCTCCTTTGAGTGATTCATTTGCTTATGTAAATGCTTTGGAAGTTGTCTCT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         370 CCACTTCATCGCCCATTCAGCTAAATTAACCATCTCCCTTGGAAG-AGAT    418
                     |.|||||          ||        ||.|||.|.|.|   ||| |.||
MutationRev        6 CAACTTC----------CA--------AAGCATTTACAT---AAGCAAAT     34

Wildtype         419 GGATCGATTACAGGCCTAGCACAAAG-------AACTTCA----------    451
                     |.|||..|.|.|||   || |.||||       ||..|||          
MutationRev       35 GAATCACTCAAAGG---AG-AAAAAGTGATGACAAGATCACCCGTCGTTA     80

Wildtype         452 ----AACAGAACAACACCAAAAGAGTAAACATCACTTTTCTCAGTCAATT    497
                         ||||||| |||.||..||.|| |.|||||..||||...|       
MutationRev       81 CGTTAACAGAA-AACTCCTTAACAG-ATACATCTTTTTTAGGA-------    121

Wildtype         498 GCTGCCTCCTGAAATATTCGGGATCTAGGTACCCGAAGCTCCCTTTCACT    547
                             ||.|||| |||     |.|||       |||.|..|||    
MutationRev      122 --------CTAAAAT-TTC-----CAAGG-------AGCACATTTT----    146

Wildtype         548 GCCGTGCTAACATGAGTTTGATCGAGCTCGGGTCCTGCCTTAGAAAGTCC    597
                        ||       |||| ||||...||               ||||||   
MutationRev      147 ---GT-------TGAG-TTGAAACAG---------------AGAAAG---    167

Wildtype         598 GAAATCAGCTACTTTTGCCA---TCATATTCTCATCAAGCAATATGTTTG    644
                                   ||||.|   |||||   ||||.|             
MutationRev      168 --------------TTGCTAAACTCATA---TCATAA-------------    187

Wildtype         645 CTGATTTG-ACATCACGATGTATAACTGCTTTGGCGTAGCTAGTATGAAG    693
                       ||||.| |||..|.|..|.|.||                   ||||  
MutationRev      188 --GATTGGTACACAAAGGGGGAAAA-------------------ATGA--    214

Wildtype         694 GTAGTGTAGACCTCTGGCTGCCCCAATACATATCTCCAGCCTCTGTTTCC    743
                            ||||    ||.|   |||||.|.||   |||     .||||.|
MutationRev      215 -------AGAC----GGAT---CCAATGCCTA---CCA-----AGTTTAC    242

Wildtype         744 AGCTCATACTAGGCA---TATCCGAACCATACAGATGGCTCTTAAGGGTC    790
                           ||.||||.|   |||   |||.|.|                   
MutationRev      243 ------TAATAGGGAATTTAT---AACTAGA-------------------    264

Wildtype         791 CCATTTTCCATGTACTCGTACACGAGAATCATCTCATTCTTTTCATCACA    840
                       |.|||                |||.|..|.|.||        |..|||
MutationRev      265 --AGTTT----------------GAGTAAAAACCCA--------ACAACA    288

Wildtype         841 GTATCCAATCAATGAAACAA---GATGACGA    868
                     |||  |||..||| ||||.|   .|||||.|
MutationRev      289 GTA--CAAAAAAT-AAACTATTTAATGACAA    316