Plant Crispr Database

Plant Information

Name: CRT0004-5-16

Seed stock: 5047
Seed availability: No

Relationship:
Parent: CRT0004-5

Transformation: CRT0004
transformation CRT0004 to TA537

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0004-5-16 (T1 )	CRT0004	CTG0003-1	CGR0003-1	gene : Solyc11g072910 (Mal1)	-2 bp/-2 bp Is tDNA present: No Mutation sequence: TTGGGAAGAGTTTGATAATATTGGTGTTGGGAGTTCTGTGTGTTTCTGGTTTTGAACCTGCTGATAATTTCTTAGTATTG TGGATCATCTAAAGATACTAATGTTGGCAATAGGGTCTTTATGGCTGATAAATCAGCTTCAAAGTTTTTGTCAACTTCAA AAGATATATTGGCTGATACCCCTTCAAATTCAATTACAAAAGCTAATGATTCACCCCTTTATCAAACTGCTAGAATTTTT ACTCAACAATCCAGCTATAAATTCCCAATTAGCCTGAAGGGGAGGCATTGGATTCGCCTTTATTTTTACCCGTTTGTTTA CCAAATTTATGATATGAGCACTGCAATGTTCTCTGTTTCCACTCAAAATAATGTTCTTCTTGGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2100 TGCCAAGAAGAACATTATTTTGAGTGGAAACAGAGAACATTGCAGTGCTC 2149 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TGCCAAGAAGAACATTATTTTGAGTGGAAACAGAGAACATTGCAGTGCTC 50 Wildtype 2150 ATATCATAAATTTGGTAAACAAACGGGTAAAAATAAAGGCGAATCCAATG 2199 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 ATATCATAAATTTGGTAAACAAACGGGTAAAAATAAAGGCGAATCCAATG 100 Wildtype 2200 CCTCCCCTTCAGGCTAATTGGGAATTTATAGCTGGATTGTTGAGTAAAAA 2249 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CCTCCCCTTCAGGCTAATTGGGAATTTATAGCTGGATTGTTGAGTAAAAA 150 Wildtype 2250 TTCTAGCAGTTTGATAAAGGGGTGAATCATTAGCTTTTGTAATTGAATTT 2299 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TTCTAGCAGTTTGATAAAGGGGTGAATCATTAGCTTTTGTAATTGAATTT 200 Wildtype 2300 GAAGGGGTATCAGCCAATATATCTTTTGAAGTTGACAAAAACTTTGAAGC 2349 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GAAGGGGTATCAGCCAATATATCTTTTGAAGTTGACAAAAACTTTGAAGC 250 Wildtype 2350 TGATTTATCAGCCATAAAGACCCTATTGCCAACATTAGTATCTTTAGATG 2399 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TGATTTATCAGCCATAAAGACCCTATTGCCAACATTAGTATCTTTAGATG 300 Wildtype 2400 ATCCACAATCTACTAAGAAATTATCAGCAGGTTCAAAACCAGAAACACAC 2449 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 ATCCACA----ACTAAGAAATTATCAGCAGGTTCAAAACCAGAAACACAC 346 gRNA CAATCTACTAAGAAATTATC Wildtype 2450 AGAACTCCCAACACCAATATTATCAAACTCTTCCCAA 2486 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 347 AGAACTCCCAACACCAATATTATCAAACTCTTCCCAA 383
CRT0004-5-16 (T1 )	CRT0004	CTG0003-2	CGR0003-2	gene : Solyc06g005230 (Mal2)	-6/WT

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0004-5-16 (T1 )

CRT0004

CTG0003-1

CGR0003-1

gene : Solyc11g072910 (Mal1)

-2 bp/-2 bp

Is tDNA present: No

Mutation sequence:

TTGGGAAGAGTTTGATAATATTGGTGTTGGGAGTTCTGTGTGTTTCTGGTTTTGAACCTGCTGATAATTTCTTAGTATTG
TGGATCATCTAAAGATACTAATGTTGGCAATAGGGTCTTTATGGCTGATAAATCAGCTTCAAAGTTTTTGTCAACTTCAA
AAGATATATTGGCTGATACCCCTTCAAATTCAATTACAAAAGCTAATGATTCACCCCTTTATCAAACTGCTAGAATTTTT
ACTCAACAATCCAGCTATAAATTCCCAATTAGCCTGAAGGGGAGGCATTGGATTCGCCTTTATTTTTACCCGTTTGTTTA
CCAAATTTATGATATGAGCACTGCAATGTTCTCTGTTTCCACTCAAAATAATGTTCTTCTTGGCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2100 TGCCAAGAAGAACATTATTTTGAGTGGAAACAGAGAACATTGCAGTGCTC   2149
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TGCCAAGAAGAACATTATTTTGAGTGGAAACAGAGAACATTGCAGTGCTC     50

Wildtype        2150 ATATCATAAATTTGGTAAACAAACGGGTAAAAATAAAGGCGAATCCAATG   2199
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 ATATCATAAATTTGGTAAACAAACGGGTAAAAATAAAGGCGAATCCAATG    100

Wildtype        2200 CCTCCCCTTCAGGCTAATTGGGAATTTATAGCTGGATTGTTGAGTAAAAA   2249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 CCTCCCCTTCAGGCTAATTGGGAATTTATAGCTGGATTGTTGAGTAAAAA    150

Wildtype        2250 TTCTAGCAGTTTGATAAAGGGGTGAATCATTAGCTTTTGTAATTGAATTT   2299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 TTCTAGCAGTTTGATAAAGGGGTGAATCATTAGCTTTTGTAATTGAATTT    200

Wildtype        2300 GAAGGGGTATCAGCCAATATATCTTTTGAAGTTGACAAAAACTTTGAAGC   2349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GAAGGGGTATCAGCCAATATATCTTTTGAAGTTGACAAAAACTTTGAAGC    250

Wildtype        2350 TGATTTATCAGCCATAAAGACCCTATTGCCAACATTAGTATCTTTAGATG   2399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 TGATTTATCAGCCATAAAGACCCTATTGCCAACATTAGTATCTTTAGATG    300

Wildtype        2400 ATCCACAATCTACTAAGAAATTATCAGCAGGTTCAAAACCAGAAACACAC   2449
                     |||||||    |||||||||||||||||||||||||||||||||||||||
MutationRev      301 ATCCACA----ACTAAGAAATTATCAGCAGGTTCAAAACCAGAAACACAC    346
gRNA                      CAATCTACTAAGAAATTATC

Wildtype        2450 AGAACTCCCAACACCAATATTATCAAACTCTTCCCAA   2486
                     |||||||||||||||||||||||||||||||||||||
MutationRev      347 AGAACTCCCAACACCAATATTATCAAACTCTTCCCAA    383

CRT0004-5-16 (T1 )

CRT0004

CTG0003-2

CGR0003-2

gene : Solyc06g005230 (Mal2)

-6/WT