Is tDNA present: No

Mutation sequence:

TCTTTTGTTCTCTCTGCATTTCCTCTCTCTAACCAAAAAGTCAAGGCCGTAGCCAAGAAATTAACAAAATGGAAGAGGAT
TGGGATCTACATGCGGTGGTTCAGAGGCTGCGCCGCTAGTTCCACCGCCGCCGCTGCTGCCGCCACCACCACAGCCACCA
CTAATTCTTGTTGCAGCAGCTTCCAACCAAGACAAGATGGCAACTTTTTTAGTTTTCAAGATCCATTTGTCCCAAGATTT
GATAACCCCATAAGTGATTTTGAAGAGTTGCATAATCTTTACAAGCCTTTCTTCCCTAAAGCACAACAAATACCTCTTTC
TCCTCAAAATAATACTAATAATATACCCATTTCACCTCTCTCTGTTCTTGGAGGACTACAAGATCTATCACCCCAACAAA
CACTAAAACAACAACAACATATTCATCAGCTAAACAGTACAAGATTAACACAACCCAAACAATCTCCTCTCTCTGCATAT
GGTTCCACAACTACTAGTGCTTCACATGTTCAAAGCCCAAGACCTAAAAGAAGGTAACTTTAACAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype          65 TCTCTGCATTTCCTCTCTCTAACCAAAAAGTCAAGGCCGTAGCCAAGAAA    114
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TCTCTGCATTTCCTCTCTCTAACCAAAAAGTCAAGGCCGTAGCCAAGAAA     50

Wildtype         115 TTAACAAAATGGAAGAGGATTGGGATCTACATGCGGTGG-TCAGAGGCTG    163
                     ||||||||||||||||||||||||||||||||||||||| ||||||||||
Mutation          51 TTAACAAAATGGAAGAGGATTGGGATCTACATGCGGTGGTTCAGAGGCTG    100
gRNA                                        GATCTACATGCGGTGG-TCAG

Wildtype         164 CGCCGCTAGTTCCACCGCCGCCGCTGCTGCCGCCACCACCACAGCCACCA    213
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 CGCCGCTAGTTCCACCGCCGCCGCTGCTGCCGCCACCACCACAGCCACCA    150

Wildtype         214 CTAATTCTTGTTGCAGCAGCTTCCAACCAAGACAAGATGGCAACTTTTTT    263
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTAATTCTTGTTGCAGCAGCTTCCAACCAAGACAAGATGGCAACTTTTTT    200

Wildtype         264 AGTTTTCAAGATCCATTTGTCCCAAGATTTGATAACCCCATAAGTGATTT    313
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 AGTTTTCAAGATCCATTTGTCCCAAGATTTGATAACCCCATAAGTGATTT    250

Wildtype         314 TGAAGAGTTGCATAATCTTTACAAGCCTTTCTTCCCTAAAGCACAACAAA    363
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAAGAGTTGCATAATCTTTACAAGCCTTTCTTCCCTAAAGCACAACAAA    300

Wildtype         364 TACCTCTTTCTCCTCAAAATAATACTAATAATATACCCATTTCACCTCTC    413
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TACCTCTTTCTCCTCAAAATAATACTAATAATATACCCATTTCACCTCTC    350

Wildtype         414 TCTGTTCTTGGAGGACTACAAGATCTATCACCCCAACAAACACTAAAACA    463
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TCTGTTCTTGGAGGACTACAAGATCTATCACCCCAACAAACACTAAAACA    400

Wildtype         464 ACAACAACATATTCATCAGCTAAACAGTACAAGATTAACACAACCCAAAC    513
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 ACAACAACATATTCATCAGCTAAACAGTACAAGATTAACACAACCCAAAC    450

Wildtype         514 AATCTCCTCTCTCTGCATATGGTTCCACAACTACTAGTGCTTCACATGTT    563
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 AATCTCCTCTCTCTGCATATGGTTCCACAACTACTAGTGCTTCACATGTT    500

Wildtype         564 CAAAGCCCAAGACCTAA    580
                     |||||||||||||||||
Mutation         501 CAAAGCCCAAGACCTAA    517

Is tDNA present: No

Mutation sequence:

CAAAAACCAGCTAAAAATACACGCATACAACACTCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCC
CCTCACTCTTTACCTCAAAAAAGTCAGCGCCTTCTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGG
ATAAAGCAGAGCAAAAACAGAGAGAGACTATGGAAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACC
ACAACCGCCGCGCGACATCAGTTTTACCAACTTCCAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTG
TATTTTGCTTTGAAGATCTGGTTCTCAAACAGAGCCTAGTATTGAATAGAAATAAAATTAGTGATGATTTCGAAGAATTG
CATCAACTTTGCAAGCCATTTATTAATCAACCATCCGAAAAATTAACGGTCATTTCATCACAATCACCGTCGCAACAGCT
TACTGATACGAAATCAATTCAACCCAACAGAACTTTATCATGTAATTTACATGCTCAAAGTCAAACAATCAAAAGAAGGT
AAATTATAATCACTATTA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1654 TTCAATACTAGGCTCTGTTTGAGAACCAGATCTTCAAAGCAAAATACAGA   1703
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TTCAATACTAGGCTCTGTTTGAGAACCAGATCTTCAAAGCAAAATACAGA     50

Wildtype        1704 GTTATTGTTACTGTTATTATCATCATCTGTTGTTCTTGGTTGGAAGTTGG   1753
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GTTATTGTTACTGTTATTATCATCATCTGTTGTTCTTGGTTGGAAGTTGG    100
gRNA                                                                 GG

Wildtype        1754 TAAAACTGATGTCGCCGGCGGTTGTGGTGGCGGTGCAGCTTCTGACGACG   1803
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 TAAAACTGATGTCGCCGGCGGTTGTGGTGGCGGTGCAGCTTCTGACGACG    150
gRNA                 GGTAAAACTGATGTCGCCGG

Wildtype        1804 GCGTTTAGATCCCAATCTTCTTCCATAGTCTCTCTCTGTTTTTGCTCTGC   1853
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GCGTTTAGATCCCAATCTTCTTCCATAGTCTCTCTCTGTTTTTGCTCTGC    200

Wildtype        1854 TTTATCCTTGACTTTTTCTTTTCTTTTTTTAGAGAGAAGAAGAAGACAAA   1903
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 TTTATCCTTGACTTTTTCTTTTCTTTTTTTAGAGAGAAGAAGAAGACAAA    250

Wildtype        1904 AGAAGGCGCTGACTTTTTTGAGGTAAAGAGTGAGGGGAT   1942
                     |||||||||||||||||||||||||||||||||||||||
MutationRev      251 AGAAGGCGCTGACTTTTTTGAGGTAAAGAGTGAGGGGAT    289