Plant Crispr Database

Plant Information

Name: CRT0009-9

Seed stock: NA
Seed availability: No

Relationship:

Transformation: CRT0009
transformation CRT0009 to RG-prf3

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0009-9 (T0 )	CRT0009	CTG0009-1	CGR0009-1	gene : Solyc04g009640 (FLS3)	-1 bp/-1 bp Is tDNA present: Yes Mutation sequence: TATGGTCATATAAATCAAAATAGAAACACAAAACGACAACTTTTCGTCTTGTGAAATAACTCAAGTGGAGTCATTCATTC CAATATATCCTTTTTCCATTTCTCTTCATATATTTCAACTTTCAAATGCTTAGTAACATCATGGAGAAACACATTTTCTT ATTGATACTTGCTATCTTAGTTCAATTTTACTTTGTTTCTTCTATATCAGCTACTATTTCCTCAAATGAGACTGATCAAG AAGCTCTACTAGCTTTTCGAAACCTTGTACGAGTGATTCTAGTCATTTTTTAGCCAATAATTGGACAAAAAACACTTCAT TTTGCTCTTGGTTTGGTGTCACTTGTAGTCCAAAAAGGCAAAGGGTTGTAGCCTTGACTCTTCCTAATTTGCAACTTCAA GGCACAATTTCGCCGTCTTTGGCCAATCTATCTTTTCTCATAGAGCTAAATCTCGCAAACAACAACTTACACAGTGAAAT CCCTGATGGCATTGGCCGCTTGCCTCGTCTACGAGTGATTGATATTCAGAACAATCAGCTGCATGGAAGTATTCCAACAA GTCTATTTCAACACGGGAGTGTTCAAATCATTTCATTGGCTTTCAATAAACTCGGTGGTGAAATGTGGAACGGTACATGG TATGTACCCGAACTCAGAGTCTTAAATCTCAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 3080 AGGAGGGATCACACCTGTAATGGTATTGTTCCTGAGATTTAAGACTCTGA 3129 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 AGGAGGGATCACACCTGTAATGGTATTGTTCCTGAGATTTAAGACTCTGA 50 Wildtype 3130 GTTCGGGTACATACCATGTACCGTTCCACATTTCACCACCGAGTTTATTG 3179 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GTTCGGGTACATACCATGTACCGTTCCACATTTCACCACCGAGTTTATTG 100 Wildtype 3180 AAAGCCAATGAAATGATTTGAACACTCCCGTGTTGAAATAGACTTGTTGG 3229 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AAAGCCAATGAAATGATTTGAACACTCCCGTGTTGAAATAGACTTGTTGG 150 Wildtype 3230 AATACTTCCATGCAGCTGATTGTTCTGAATATCAATCACTCGTAGACGAG 3279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 AATACTTCCATGCAGCTGATTGTTCTGAATATCAATCACTCGTAGACGAG 200 Wildtype 3280 GCAAGCGGCCAATGCCATCAGGGATTTCACTGTGTAAGTTGTTGTTTGCG 3329 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GCAAGCGGCCAATGCCATCAGGGATTTCACTGTGTAAGTTGTTGTTTGCG 250 Wildtype 3330 AGATTTAGCTCTATGAGAAAAGATAGATTGGCCAAAGACGGCGAAATTGT 3379 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 AGATTTAGCTCTATGAGAAAAGATAGATTGGCCAAAGACGGCGAAATTGT 300 Wildtype 3380 GCCTTGAAGTTGCAAATTAGGAAGAGTCAAGGCTACAACCCTTTGCCTTT 3429 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 GCCTTGAAGTTGCAAATTAGGAAGAGTCAAGGCTACAACCCTTTGCCTTT 350 Wildtype 3430 TTGGACTACAAGTGACACCAAACCAAGAGCAAAATGAAGTGTTTTTTGTC 3479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 TTGGACTACAAGTGACACCAAACCAAGAGCAAAATGAAGTGTTTTTTGTC 400 Wildtype 3480 CAATTATTGGCTAAAAAATGACTAGAATCACTCGTA-ACAAGGTTTCGAA 3528 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 CAATTATTGGCTAAAAAATGACTAGAATCACTCGTAGACAAGGTTTCGAA 450 gRNA GACTAGAATCACTCGTA-ACA Wildtype 3529 AAGCTAGTAGAGCTTCTTGATCAGTCTCATTTGAGGAAATAGTAGCTGAT 3578 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 AAGCTAGTAGAGCTTCTTGATCAGTCTCATTTGAGGAAATAGTAGCTGAT 500 Wildtype 3579 ATAGAAGAAACAAAGTAAAATTGAACTAAGATAGCAAGTATCAATAAGAA 3628 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 ATAGAAGAAACAAAGTAAAATTGAACTAAGATAGCAAGTATCAATAAGAA 550 Wildtype 3629 AATGTGTTTCTCCATGATGTTACTAAGCATTTGA 3662 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 551 AATGTGTTTCTCCATGATGTTACTAAGCATTTGA 584

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0009-9 (T0 )

CRT0009

CTG0009-1

CGR0009-1

gene : Solyc04g009640 (FLS3)

-1 bp/-1 bp

Is tDNA present: Yes

Mutation sequence:

TATGGTCATATAAATCAAAATAGAAACACAAAACGACAACTTTTCGTCTTGTGAAATAACTCAAGTGGAGTCATTCATTC
CAATATATCCTTTTTCCATTTCTCTTCATATATTTCAACTTTCAAATGCTTAGTAACATCATGGAGAAACACATTTTCTT
ATTGATACTTGCTATCTTAGTTCAATTTTACTTTGTTTCTTCTATATCAGCTACTATTTCCTCAAATGAGACTGATCAAG
AAGCTCTACTAGCTTTTCGAAACCTTGTACGAGTGATTCTAGTCATTTTTTAGCCAATAATTGGACAAAAAACACTTCAT
TTTGCTCTTGGTTTGGTGTCACTTGTAGTCCAAAAAGGCAAAGGGTTGTAGCCTTGACTCTTCCTAATTTGCAACTTCAA
GGCACAATTTCGCCGTCTTTGGCCAATCTATCTTTTCTCATAGAGCTAAATCTCGCAAACAACAACTTACACAGTGAAAT
CCCTGATGGCATTGGCCGCTTGCCTCGTCTACGAGTGATTGATATTCAGAACAATCAGCTGCATGGAAGTATTCCAACAA
GTCTATTTCAACACGGGAGTGTTCAAATCATTTCATTGGCTTTCAATAAACTCGGTGGTGAAATGTGGAACGGTACATGG
TATGTACCCGAACTCAGAGTCTTAAATCTCAG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        3080 AGGAGGGATCACACCTGTAATGGTATTGTTCCTGAGATTTAAGACTCTGA   3129
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 AGGAGGGATCACACCTGTAATGGTATTGTTCCTGAGATTTAAGACTCTGA     50

Wildtype        3130 GTTCGGGTACATACCATGTACCGTTCCACATTTCACCACCGAGTTTATTG   3179
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GTTCGGGTACATACCATGTACCGTTCCACATTTCACCACCGAGTTTATTG    100

Wildtype        3180 AAAGCCAATGAAATGATTTGAACACTCCCGTGTTGAAATAGACTTGTTGG   3229
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AAAGCCAATGAAATGATTTGAACACTCCCGTGTTGAAATAGACTTGTTGG    150

Wildtype        3230 AATACTTCCATGCAGCTGATTGTTCTGAATATCAATCACTCGTAGACGAG   3279
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 AATACTTCCATGCAGCTGATTGTTCTGAATATCAATCACTCGTAGACGAG    200

Wildtype        3280 GCAAGCGGCCAATGCCATCAGGGATTTCACTGTGTAAGTTGTTGTTTGCG   3329
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GCAAGCGGCCAATGCCATCAGGGATTTCACTGTGTAAGTTGTTGTTTGCG    250

Wildtype        3330 AGATTTAGCTCTATGAGAAAAGATAGATTGGCCAAAGACGGCGAAATTGT   3379
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 AGATTTAGCTCTATGAGAAAAGATAGATTGGCCAAAGACGGCGAAATTGT    300

Wildtype        3380 GCCTTGAAGTTGCAAATTAGGAAGAGTCAAGGCTACAACCCTTTGCCTTT   3429
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 GCCTTGAAGTTGCAAATTAGGAAGAGTCAAGGCTACAACCCTTTGCCTTT    350

Wildtype        3430 TTGGACTACAAGTGACACCAAACCAAGAGCAAAATGAAGTGTTTTTTGTC   3479
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 TTGGACTACAAGTGACACCAAACCAAGAGCAAAATGAAGTGTTTTTTGTC    400

Wildtype        3480 CAATTATTGGCTAAAAAATGACTAGAATCACTCGTA-ACAAGGTTTCGAA   3528
                     |||||||||||||||||||||||||||||||||||| |||||||||||||
MutationRev      401 CAATTATTGGCTAAAAAATGACTAGAATCACTCGTAGACAAGGTTTCGAA    450
gRNA                                    GACTAGAATCACTCGTA-ACA

Wildtype        3529 AAGCTAGTAGAGCTTCTTGATCAGTCTCATTTGAGGAAATAGTAGCTGAT   3578
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 AAGCTAGTAGAGCTTCTTGATCAGTCTCATTTGAGGAAATAGTAGCTGAT    500

Wildtype        3579 ATAGAAGAAACAAAGTAAAATTGAACTAAGATAGCAAGTATCAATAAGAA   3628
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      501 ATAGAAGAAACAAAGTAAAATTGAACTAAGATAGCAAGTATCAATAAGAA    550

Wildtype        3629 AATGTGTTTCTCCATGATGTTACTAAGCATTTGA   3662
                     ||||||||||||||||||||||||||||||||||
MutationRev      551 AATGTGTTTCTCCATGATGTTACTAAGCATTTGA    584