Is tDNA present: No

Mutation sequence:

GTAGCTTATACAAACAATTTGGTTGGTTCTATTCCATTGGAATGCTGACAGCTTTGCATACCCTTGATCTGAGCGAAAAC
CAGTTATCTGGACCTATACCACCAGAAATTGGCAACTTGTCAAGTTTAGGAATTCTTCAGTTACATCTCAACTCTCTTTC
TGGGAAAATCCCATCTGAACTTGGCCTCTGTATCAATCTTTTTACCTTGAACATGTATACTAATCAATTTACTGGAAGCA
TACCTCCTGAGCTTGGAAATTTAGAAAACTTGCAAATGCTCAGATTGTATAACAATAAGTTGAACTCCAGTATAC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         562 AGGCTTCAACAACAACAATTTCACAGGCAAATTACCATCTGAAATTGGTA    611
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 AGGCTTCAACAACAACAATTTCACAGGCAAATTACCATCTGAAATTGGTA     50

Wildtype         612 ATTTGGCTAATCTTCAGTTGTTTGTAGCTTATACAAACAATTTGGTTGGT    661
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATTTGGCTAATCTTCAGTTGTTTGTAGCTTATACAAACAATTTGGTTGGT    100

Wildtype         662 TTTATGCCTACC-TCCATTGGAATGCTGACAGCTTTGCATACCCTTGATC    710
                     |||||||||||| |||||||||||||||||||||||||||||||||||||
Mutation         101 TTTATGCCTACCTTCCATTGGAATGCTGACAGCTTTGCATACCCTTGATC    150
gRNA                          ACC-TCCATTGGAATGCTGAC

Wildtype         711 TGAGCGAAAACCAGTTATCTGGACCTATACCACCAGAAATTGGCAACTTG    760
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TGAGCGAAAACCAGTTATCTGGACCTATACCACCAGAAATTGGCAACTTG    200

Wildtype         761 TCAAGTTTAGGAATTCTTCAGTTACATCTCAACTCTCTTTCTGGGAAAAT    810
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TCAAGTTTAGGAATTCTTCAGTTACATCTCAACTCTCTTTCTGGGAAAAT    250

Wildtype         811 CCCATCTGAACTTGGCCTCTGTATCAATCTTTTTACCTTGAACATGTATA    860
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CCCATCTGAACTTGGCCTCTGTATCAATCTTTTTACCTTGAACATGTATA    300

Wildtype         861 CTAATCAATTTACTGGAAGCATACCTCCTGAGCTTGGAAATTTAGAAAAC    910
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 CTAATCAATTTACTGGAAGCATACCTCCTGAGCTTGGAAATTTAGAAAAC    350

Wildtype         911 TTGCAAATGCTCAGATTGTATAACAATAAGTTGAACTCCAGTATACCTGC    960
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TTGCAAATGCTCAGATTGTATAACAATAAGTTGAACTCCAGTATACCTGC    400

Wildtype         961 CTCAATATTCCACCTGAAGTCATTAACTCATTTAGGACTCTCACAGAATG   1010
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 CTCAATATTCCACCTGAAGTCATTAACTCATTTAGGACTCTCACAGAATG    450

Wildtype        1011 AGCTAACTGGGAATATTCCT   1030
                     ||||||||||||||||||||
Mutation         451 AGCTAACTGGGAATATTCCT    470

Is tDNA present: No

Mutation sequence:

TCCTACCTCCATTGGAGCACTGCAAACCCTTGATCTGAGCTCAAACCAGTTTTCTGGACCTATACCACCAGAAATTGGCA
ACTTGTCAAGTTTAGAAATTCTTCAGTTGCATCACAACTTTCTTTCTGGGAAAATCCCATCTGAACTTGGCCTCTGTATC
AATCTTGTTACCTTGGACATGTATAATAACCAATTCACTGGAGGCGTACCTCCTGAGCTTGGG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         519 AAGCCTCATCAACAACAGCCTCACAGGCAAATTACCGTCTGAAATTGGTA    568
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 AAGCCTCATCAACAACAGCCTCACAGGCAAATTACCGTCTGAAATTGGTA     50

Wildtype         569 ATTTGGCCAATCTTCAATTGTTAGAAGCTTATAGAAACAATTTGGTTGGT    618
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATTTGGCCAATCTTCAATTGTTAGAAGCTTATAGAAACAATTTGGTTGGT    100

Wildtype         619 TCTATTCCTA-CCTCCATTGGAATGCTGACAGCACTGCAAACCCTTGATC    667
                     |||||||||| |||||||||||||||||||||||||||||||||||||||
Mutation         101 TCTATTCCTATCCTCCATTGGAATGCTGACAGCACTGCAAACCCTTGATC    150
gRNA                          A-CCTCCATTGGAATGCTGAC

Wildtype         668 TGAGCTCAAACCAGTTTTCTGGACCTATACCACCAGAAATTGGCAACTTG    717
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TGAGCTCAAACCAGTTTTCTGGACCTATACCACCAGAAATTGGCAACTTG    200

Wildtype         718 TCAAGTTTAGAAATTCTTCAGTTGCATCACAACTTTCTTTCTGGGAAAAT    767
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TCAAGTTTAGAAATTCTTCAGTTGCATCACAACTTTCTTTCTGGGAAAAT    250

Wildtype         768 CCCATCTGAACTTGGCCTCTGTATCAATCTTGTTACCTTGGACATGTATA    817
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CCCATCTGAACTTGGCCTCTGTATCAATCTTGTTACCTTGGACATGTATA    300

Wildtype         818 ATAACCAATTCACTGGAGGCGTACCTCCTGAGCTTGGGAATTTAGAAAAC    867
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 ATAACCAATTCACTGGAGGCGTACCTCCTGAGCTTGGGAATTTAGAAAAC    350

Wildtype         868 CTGCACAGGCTCAGATTGTATAACAATAAGTTGAACTCCAGTATACCTGC    917
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 CTGCACAGGCTCAGATTGTATAACAATAAGTTGAACTCCAGTATACCTGC    400

Wildtype         918 CTCATTGTTCCATTTGAAGTCATTAACTCATTTAGGACTCTCACATAATG    967
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 CTCATTGTTCCATTTGAAGTCATTAACTCATTTAGGACTCTCACATAATG    450

Wildtype         968 AGCAAACTGGTAAAATTCCTCCTGAGTTTGGATCTTTAATGTCACTACAA   1017
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 AGCAAACTGGTAAAATTCCTCCTGAGTTTGGATCTTTAATGTCACTACAA    500

Wildtype        1018 GTGCTTACCCTCCAC   1032
                     |||||||||||||||
Mutation         501 GTGCTTACCCTCCAC    515