Plant Crispr Database

Plant Information

Name: CRT0011-10-5

Seed stock: 5057
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0011-10
Progeny: CRT0011-10-5-1

Transformation: CRT0011
transformation CRT0011 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0011-10-5 (T1 )	CRT0011	CTG0011-1	CGR0011-1	gene : Solyc04g082260 (Mai1)	-4 bp/-4 bp Is tDNA present: Yes Mutation sequence: ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGTGGAACCTTAGTAGCTCAGTTACATGGCTAT ATCCACTATATCCATTCAGGTCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAAAAACACAAGG GCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGCAAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTC TTAACAGCGATCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTTTCTCCACTTTCAGAAACAAT AAATTCTGAACTGAAGTTATTAGTAGCTGCTTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC CGACAGCAGCTCCGGCGCCGGTGCCGTTA----CTCGTGCGTTTACCACCCCATGACGCTGATCTTTCTCTGAGCTCAAC TCCTTCAAGATTGAAGATTGACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCATAACCAATCTT AAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATACAACTTTATTATATGATTAAATTAATCATAAAAATAGCTT TTTTAATC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 5027 ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGT 5076 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGT 50 Wildtype 5077 GGAACCTTAGTAGCTCAGTTACATGGCTATATCCACTATATCCATTCAGG 5126 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 GGAACCTTAGTAGCTCAGTTACATGGCTATATCCACTATATCCATTCAGG 100 Wildtype 5127 TCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAA 5176 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAA 150 Wildtype 5177 TAAAACAAGGGCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGC 5226 .\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 AAACACAAGGGCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGC 200 Wildtype 5227 AAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTCTTAACAGCGA 5276 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 AAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTCTTAACAGCGA 250 Wildtype 5277 TCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTT 5326 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 TCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTT 300 Wildtype 5327 TCTCCACTTTCAGAAACAATAAATTCTGAACTGAAGTTATTCGTAGCTGC 5376 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\| Mutation 301 TCTCCACTTTCAGAAACAATAAATTCTGAACTGAAGTTATTAGTAGCTGC 350 Wildtype 5377 TTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC 5426 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 351 TTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC 400 Wildtype 5427 CGACAGCAGCTCCGGCGCCGGTGCCGTTAGAAGCTCGTGCGTTTACCACC 5476 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 401 CGACAGCAGCTCCGGCGCCGGTGCCGTTA----CTCGTGCGTTTACCACC 446 gRNA TTAGAAGCTCGTGCGTTTAC Wildtype 5477 CCATGACGCTGATCTTTCTCTGAGCTCAACTCCTTCAAGATTGAAGATTG 5526 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 447 CCATGACGCTGATCTTTCTCTGAGCTCAACTCCTTCAAGATTGAAGATTG 496 Wildtype 5527 ACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCAT 5576 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 497 ACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCAT 546 Wildtype 5577 AACCAATCTTAAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATA 5626 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 547 AACCAATCTTAAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATA 596 Wildtype 5627 CAACTTTATTATATGATTAAATTAATCATAAAAATAGCTTTTTTAATC 5674 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 597 CAACTTTATTATATGATTAAATTAATCATAAAAATAGCTTTTTTAATC 644	Morphology : "Slow growth, sparse, brittle leaves" Immunity response: Inconclusive disease assays with Pst DC3000 due to leaf phenotype.

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0011-10-5 (T1 )

CRT0011

CTG0011-1

CGR0011-1

gene : Solyc04g082260 (Mai1)

-4 bp/-4 bp

Is tDNA present: Yes

Mutation sequence:

ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGTGGAACCTTAGTAGCTCAGTTACATGGCTAT
ATCCACTATATCCATTCAGGTCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAAAAACACAAGG
GCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGCAAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTC
TTAACAGCGATCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTTTCTCCACTTTCAGAAACAAT
AAATTCTGAACTGAAGTTATTAGTAGCTGCTTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC
CGACAGCAGCTCCGGCGCCGGTGCCGTTA----CTCGTGCGTTTACCACCCCATGACGCTGATCTTTCTCTGAGCTCAAC
TCCTTCAAGATTGAAGATTGACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCATAACCAATCTT
AAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATACAACTTTATTATATGATTAAATTAATCATAAAAATAGCTT
TTTTAATC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        5027 ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGT   5076
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGT     50

Wildtype        5077 GGAACCTTAGTAGCTCAGTTACATGGCTATATCCACTATATCCATTCAGG   5126
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GGAACCTTAGTAGCTCAGTTACATGGCTATATCCACTATATCCATTCAGG    100

Wildtype        5127 TCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAA   5176
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAA    150

Wildtype        5177 TAAAACAAGGGCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGC   5226
                     .||.||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 AAACACAAGGGCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGC    200

Wildtype        5227 AAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTCTTAACAGCGA   5276
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 AAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTCTTAACAGCGA    250

Wildtype        5277 TCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTT   5326
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTT    300

Wildtype        5327 TCTCCACTTTCAGAAACAATAAATTCTGAACTGAAGTTATTCGTAGCTGC   5376
                     |||||||||||||||||||||||||||||||||||||||||.||||||||
Mutation         301 TCTCCACTTTCAGAAACAATAAATTCTGAACTGAAGTTATTAGTAGCTGC    350

Wildtype        5377 TTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC   5426
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC    400

Wildtype        5427 CGACAGCAGCTCCGGCGCCGGTGCCGTTAGAAGCTCGTGCGTTTACCACC   5476
                     |||||||||||||||||||||||||||||    |||||||||||||||||
Mutation         401 CGACAGCAGCTCCGGCGCCGGTGCCGTTA----CTCGTGCGTTTACCACC    446
gRNA                                           TTAGAAGCTCGTGCGTTTAC

Wildtype        5477 CCATGACGCTGATCTTTCTCTGAGCTCAACTCCTTCAAGATTGAAGATTG   5526
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         447 CCATGACGCTGATCTTTCTCTGAGCTCAACTCCTTCAAGATTGAAGATTG    496

Wildtype        5527 ACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCAT   5576
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         497 ACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCAT    546

Wildtype        5577 AACCAATCTTAAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATA   5626
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         547 AACCAATCTTAAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATA    596

Wildtype        5627 CAACTTTATTATATGATTAAATTAATCATAAAAATAGCTTTTTTAATC   5674
                     ||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         597 CAACTTTATTATATGATTAAATTAATCATAAAAATAGCTTTTTTAATC    644

Morphology : "Slow growth, sparse, brittle leaves"
Immunity response: Inconclusive disease assays with Pst DC3000 due to leaf phenotype.