Plant Crispr Database

Plant Information

Name: CRT0012-5-8

Seed stock: 5395
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0012-5

Transformation: CRT0012
transformation CRT0012 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0012-5-8 (T1 )	CRT0012	CTG0012-2	CGR0012-2	gene : Solyc12g099830 (BSK830)	-2 bp/-2 bp Is tDNA present: Yes Mutation sequence: CGGGTGTCTAACTAAGATAACCTTACAAGAAGCACAATGCACTTGAATTGACAATTTGGTTCAATGCAGCCAAATGCAGA TCTTGAAGCCGCATAAAGTGTAATTTGTCGGGTTGTGCTACAAACTCCAAGCTACTATATCTTTAAAGACTTAAAATCTG TAGGATAGTGTGATGGGCTGTGAAAGTTCTAAACTTGCCTCATGTTGCTGGACTGGAGAGAGTGGCCCCATACGAGGCTC AGAATCCTGGTATGTATATCTTTTATTGAAATATTTTCTATATAACCATCCTACTGCAGTGTTTAATAAATTTTTTTTCA CGCTTCTCGTAGATGAAGAAAAAAATGAAGTTAGTGATTCACCTGCATTCTGCGAGTTTACATTTGAGCAGCTCAGGATA GCTACATCTGCATTTGCTGTAGAGAATATAGTTTCTGAACATGGCGAAAAAGCTCCAAATGTTGTTTATAAAGGAAAGCT AGAGAACCAGAGGCCGGTTGCTGTTAAACGCTTCAACAGATCTGCATGGCCTGATTCCCGGCAATTTTTAGTAAGAGCTT GTCTGTTAATTCCTTGGTATATTTCAACTCTATGGAAAAGACGTTCCCATATCCATCTACTAAAATGATATAATTTCCTT TTTCTTTTCTTATATTAAATGTTGACGATGATATTCATGACAAGAATAGAAATTTAAAATAAAATAGTAATGAGAATAAT TG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 4729 AGAAAAAGGAAATTATATCATTTTAGTAGATGGATATGGGAACGTCTTTT 4778 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 AGAAAAAGGAAATTATATCATTTTAGTAGATGGATATGGGAACGTCTTTT 50 Wildtype 4779 CCATAGAGTTGAAATATACCAAGGAATTAACAGACAAGCTCTTACTAAAA 4828 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CCATAGAGTTGAAATATACCAAGGAATTAACAGACAAGCTCTTACTAAAA 100 Wildtype 4829 ATTGCCGGGAATCAGGCCATGCAGATCTGTTGAAGCGTTTAACAGCAACC 4878 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 ATTGCCGGGAATCAGGCCATGCAGATCTGTTGAAGCGTTTAACAGCAACC 150 Wildtype 4879 GGCCTCTGGTTCTCTAGCTTTCCTTTATAAACAACATTTGGAGCTTTTTC 4928 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GGCCTCTGGTTCTCTAGCTTTCCTTTATAAACAACATTTGGAGCTTTTTC 200 Wildtype 4929 GCCATGTTCAGAAACTATATTCTCTACAGCAAATGCAGATGTAGCTATCC 4978 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GCCATGTTCAGAAACTATATTCTCTACAGCAAATGCAGATGTAGCTATCC 250 Wildtype 4979 TGAGCTGCTCAAATGTAAACTCGCAGAATGCAGGTGAATCACTAACTTCA 5028 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TGAGCTGCTCAAATGTAAACTCGCAGAATGCAGGTGAATCACTAACTTCA 300 Wildtype 5029 TTTTTTTCTTCATCTACGAGAAGCGTGAAAAAAAATTTATTAAACACTGC 5078 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 TTTTTTTCTTCATCTACGAGAAGCGTGAAAAAAAATTTATTAAACACTGC 350 Wildtype 5079 AGTAGGATGGTTATATAGAAAATATTTCAATAAAAGATATACATACCAGG 5128 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 AGTAGGATGGTTATATAGAAAATATTTCAATAAAAGATATACATACCAGG 400 Wildtype 5129 ATTCTGAGCCTCGTGAATGGGGCCACTCTCTCCAGTCCAGCAACATGAGG 5178 \|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 ATTCTGAGCCT----AATGGGGCCACTCTCTCCAGTCCAGCAACATGAGG 446 Wildtype 5179 CAAGTTTAGAACTTTCACAGCCCAT 5203 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 447 CAAGTTTAGAACTTTCACAGCCCAT 471

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0012-5-8 (T1 )

CRT0012

CTG0012-2

CGR0012-2

gene : Solyc12g099830 (BSK830)

-2 bp/-2 bp

Is tDNA present: Yes

Mutation sequence:

CGGGTGTCTAACTAAGATAACCTTACAAGAAGCACAATGCACTTGAATTGACAATTTGGTTCAATGCAGCCAAATGCAGA
TCTTGAAGCCGCATAAAGTGTAATTTGTCGGGTTGTGCTACAAACTCCAAGCTACTATATCTTTAAAGACTTAAAATCTG
TAGGATAGTGTGATGGGCTGTGAAAGTTCTAAACTTGCCTCATGTTGCTGGACTGGAGAGAGTGGCCCCATACGAGGCTC
AGAATCCTGGTATGTATATCTTTTATTGAAATATTTTCTATATAACCATCCTACTGCAGTGTTTAATAAATTTTTTTTCA
CGCTTCTCGTAGATGAAGAAAAAAATGAAGTTAGTGATTCACCTGCATTCTGCGAGTTTACATTTGAGCAGCTCAGGATA
GCTACATCTGCATTTGCTGTAGAGAATATAGTTTCTGAACATGGCGAAAAAGCTCCAAATGTTGTTTATAAAGGAAAGCT
AGAGAACCAGAGGCCGGTTGCTGTTAAACGCTTCAACAGATCTGCATGGCCTGATTCCCGGCAATTTTTAGTAAGAGCTT
GTCTGTTAATTCCTTGGTATATTTCAACTCTATGGAAAAGACGTTCCCATATCCATCTACTAAAATGATATAATTTCCTT
TTTCTTTTCTTATATTAAATGTTGACGATGATATTCATGACAAGAATAGAAATTTAAAATAAAATAGTAATGAGAATAAT
TG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        4729 AGAAAAAGGAAATTATATCATTTTAGTAGATGGATATGGGAACGTCTTTT   4778
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 AGAAAAAGGAAATTATATCATTTTAGTAGATGGATATGGGAACGTCTTTT     50

Wildtype        4779 CCATAGAGTTGAAATATACCAAGGAATTAACAGACAAGCTCTTACTAAAA   4828
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CCATAGAGTTGAAATATACCAAGGAATTAACAGACAAGCTCTTACTAAAA    100

Wildtype        4829 ATTGCCGGGAATCAGGCCATGCAGATCTGTTGAAGCGTTTAACAGCAACC   4878
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATTGCCGGGAATCAGGCCATGCAGATCTGTTGAAGCGTTTAACAGCAACC    150

Wildtype        4879 GGCCTCTGGTTCTCTAGCTTTCCTTTATAAACAACATTTGGAGCTTTTTC   4928
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GGCCTCTGGTTCTCTAGCTTTCCTTTATAAACAACATTTGGAGCTTTTTC    200

Wildtype        4929 GCCATGTTCAGAAACTATATTCTCTACAGCAAATGCAGATGTAGCTATCC   4978
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GCCATGTTCAGAAACTATATTCTCTACAGCAAATGCAGATGTAGCTATCC    250

Wildtype        4979 TGAGCTGCTCAAATGTAAACTCGCAGAATGCAGGTGAATCACTAACTTCA   5028
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 TGAGCTGCTCAAATGTAAACTCGCAGAATGCAGGTGAATCACTAACTTCA    300

Wildtype        5029 TTTTTTTCTTCATCTACGAGAAGCGTGAAAAAAAATTTATTAAACACTGC   5078
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 TTTTTTTCTTCATCTACGAGAAGCGTGAAAAAAAATTTATTAAACACTGC    350

Wildtype        5079 AGTAGGATGGTTATATAGAAAATATTTCAATAAAAGATATACATACCAGG   5128
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 AGTAGGATGGTTATATAGAAAATATTTCAATAAAAGATATACATACCAGG    400

Wildtype        5129 ATTCTGAGCCTCGTGAATGGGGCCACTCTCTCCAGTCCAGCAACATGAGG   5178
                     |||||||||||    |||||||||||||||||||||||||||||||||||
MutationRev      401 ATTCTGAGCCT----AATGGGGCCACTCTCTCCAGTCCAGCAACATGAGG    446

Wildtype        5179 CAAGTTTAGAACTTTCACAGCCCAT   5203
                     |||||||||||||||||||||||||
MutationRev      447 CAAGTTTAGAACTTTCACAGCCCAT    471