Plant Crispr Database

Plant Information

Name: CRT0016-4-4-4

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0016-4-4

Transformation: CRT0016
transformation CRT0016 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0016-4-4-4 (T2 )	CRT0016	CTG0016-1	CGR0016-1	gene : Solyc09g014720 (Wak1)	-10 bp/-10 bp Is tDNA present: No Mutation sequence: ATATATCTAAGTTTAGTCCGTCAATTAGAGTTAAGTTTTTTTTCAGGTTGTCAGTGATTCGATACGAAACTAATAATTTG CTTCAAATTCAGAAAGTGTTTTCAACAAAATCTCTCTAAATAAAACTACAAATAGGAAATAATCCAAAGATACTATTGTA CGAATCGGTTCCCCTTAAGCCCTATATATGGAACCTTTTACTCAATAAGTCCTTTTAATTTCCATAATAATATTTTTTCA AAACCATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTCTTAACACTAGCCACCGCCCAAATTATCC CTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCCCTCCCCCACCAAC ACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTCCATATCCATTTGG AATTGGGTCTGGTTGTGCCTTAGACCCCATGTTTGAGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 3224 TCTCAAACATGGGGTCTAAGGCACAACCAGACCCAATTCCAAATGGATAT 3273 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TCTCAAACATGGGGTCTAAGGCACAACCAGACCCAATTCCAAATGGATAT 50 Wildtype 3274 GGAACTGTAACGTTTCCACACTGTTTTGGACATCCTGGTTTTGTGATGTT 3323 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GGAACTGTAACGTTTCCACACTGTTTTGGACATCCTGGTTTTGTGATGTT 100 Wildtype 3324 AGCAGCTTTGGTGATTGTGTTGGTGGGGGAGGGGGCGGGGGCGGTGGCAT 3373 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AGCAGCTTTGGTGATTGTGTTGGTGGGGGAGGGGGCGGGGGCGGTGGCAT 150 Wildtype 3374 TCGTTGGCGGAGAAGTGGAGTTTGTGGGCGGAGATGTGGTGTTAGAAGGG 3423 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TCGTTGGCGGAGAAGTGGAGTTTGTGGGCGGAGATGTGGTGTTAGAAGGG 200 Wildtype 3424 ATAATTTGGGCGGTGGCTAGTGTTAAGATTAGCATAAAACAGGGGAGTTG 3473 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 ATAATTTGGGCGGTGGCTAGTGTTAAG----------AACAGGGGAGTTG 240 gRNA GTTAAGATTAGCATAAAACA Wildtype 3474 GAAAGAAAACAGAGCAACTTGATAATGCTGCAT 3506 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 241 GAAAGAAAACAGAGCAACTTGATAATGCTGCAT 273	Immunity response: More susceptible to DC3000dd

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0016-4-4-4 (T2 )

CRT0016

CTG0016-1

CGR0016-1

gene : Solyc09g014720 (Wak1)

-10 bp/-10 bp

Is tDNA present: No

Mutation sequence:

ATATATCTAAGTTTAGTCCGTCAATTAGAGTTAAGTTTTTTTTCAGGTTGTCAGTGATTCGATACGAAACTAATAATTTG
CTTCAAATTCAGAAAGTGTTTTCAACAAAATCTCTCTAAATAAAACTACAAATAGGAAATAATCCAAAGATACTATTGTA
CGAATCGGTTCCCCTTAAGCCCTATATATGGAACCTTTTACTCAATAAGTCCTTTTAATTTCCATAATAATATTTTTTCA
AAACCATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTCTTAACACTAGCCACCGCCCAAATTATCC
CTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCCCTCCCCCACCAAC
ACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTCCATATCCATTTGG
AATTGGGTCTGGTTGTGCCTTAGACCCCATGTTTGAGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        3224 TCTCAAACATGGGGTCTAAGGCACAACCAGACCCAATTCCAAATGGATAT   3273
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TCTCAAACATGGGGTCTAAGGCACAACCAGACCCAATTCCAAATGGATAT     50

Wildtype        3274 GGAACTGTAACGTTTCCACACTGTTTTGGACATCCTGGTTTTGTGATGTT   3323
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GGAACTGTAACGTTTCCACACTGTTTTGGACATCCTGGTTTTGTGATGTT    100

Wildtype        3324 AGCAGCTTTGGTGATTGTGTTGGTGGGGGAGGGGGCGGGGGCGGTGGCAT   3373
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AGCAGCTTTGGTGATTGTGTTGGTGGGGGAGGGGGCGGGGGCGGTGGCAT    150

Wildtype        3374 TCGTTGGCGGAGAAGTGGAGTTTGTGGGCGGAGATGTGGTGTTAGAAGGG   3423
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 TCGTTGGCGGAGAAGTGGAGTTTGTGGGCGGAGATGTGGTGTTAGAAGGG    200

Wildtype        3424 ATAATTTGGGCGGTGGCTAGTGTTAAGATTAGCATAAAACAGGGGAGTTG   3473
                     |||||||||||||||||||||||||||          |||||||||||||
MutationRev      201 ATAATTTGGGCGGTGGCTAGTGTTAAG----------AACAGGGGAGTTG    240
gRNA                                      GTTAAGATTAGCATAAAACA

Wildtype        3474 GAAAGAAAACAGAGCAACTTGATAATGCTGCAT   3506
                     |||||||||||||||||||||||||||||||||
MutationRev      241 GAAAGAAAACAGAGCAACTTGATAATGCTGCAT    273

Immunity response: More susceptible to DC3000dd