Plant Crispr Database

Plant Information

Name: CRT0018-5-4

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0018-5

Transformation: CRT0018
transformation CRT0018 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0018-5-4 (T1 )	CRT0018	CTG0018-2	CGR0018-2	gene : Solyc03g117980 (RbohB)	-7 bp /-7 bp Is tDNA present: Yes Mutation sequence: GAACTTAAGACCTTTAAGAGCATGAGCAGCAGCTGATTTATTCCTGTCGAACCTTCCAGTAGGAATTGGGCGCTTATTTA AGGAAGCCAATCGCTTGAGCTCTTGAGACACTTGCCGGATTCTCGAAGAAGCATTTCGTACAAGTGAAGCTCCCAAGGTA GATTTCTTCTCCAAACCTTTAGCCAATAAAGCCAACTCCGGATCTTCCACGTCAGCACCGCCGGCACACTGTGGACAGCC ACAGAGTCTTCACGAACATCGAGAGTGATTTCCACATAAGCATCGTCATTGGACTTGGCGCCGGCTCCTGCACTAGTTCC GATGTCAGTAGATTCCGGTATATTAAACCTCGCGCTCTTTTTTCCTCCTCGTTTGTTCAGCGGTCCGCTTAAAGGACCAC TGTACGACGCTCTATCATTCCCTATAACCTCCGTATCCGAGTGATGATGGTGATGGTGTGGGTGATGATTTTCCGAATTT TGCATCGCTACAATAGTTTCTTCGTGGAAAAATGAAATGAATGAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 201 TTCATTCATTTCATTTTTCCACGAAGAAACTATTGTAGCGATGCAAAATT 250 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TTCATTCATTTCATTTTTCCACGAAGAAACTATTGTAGCGATGCAAAATT 50 Wildtype 251 CGGAAAATCATCACCCACACCATCACCATCATCACTCGGATACGGAGGTT 300 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CGGAAAATCATCACCCACACCATCACCATCATCACTCGGATACGGAGGTT 100 Wildtype 301 ATAGGGAATGATAGAGCGTCGTACAGTGGTCCTTTAAGCGGACCGCTGAA 350 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 ATAGGGAATGATAGAGCGTCGTACAGTGGTCCTTTAAGCGGACCGCTGAA 150 Wildtype 351 CAAACGAGGAGGAAAAAAGAGCGCGAGGTTTAATATACCGGAATCTACTG 400 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 CAAACGAGGAGGAAAAAAGAGCGCGAGGTTTAATATACCGGAATCTACTG 200 Wildtype 401 ACATCGGAACTAGTGCAGGAGCCGGCGCCAAGTCCAATGACGATGCTTAT 450 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 ACATCGGAACTAGTGCAGGAGCCGGCGCCAAGTCCAATGACGATGCTTAT 250 Wildtype 451 GTGGAAATCACTCTCGATGTTCGTGAAGACTCTGTGGCTGTCCACAGTGT 500 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GTGGAAATCACTCTCGATGTTCGTGAAGACTCTGTGGCTGTCCACAGTG- 299 gRNA GTCCACAGTGT Wildtype 501 GAAAACTGCCGGCGGTGCTGACGTGGAAGATCCGGAGTTGGCTTTATTGG 550 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 300 ------TGCCGGCGGTGCTGACGTGGAAGATCCGGAGTTGGCTTTATTGG 343 gRNA GTCCACAGTGTGAAAACTGC Wildtype 551 CTAAAGGTTTGGAGAAGAAATCTACCTTGGGAGCTTCACTTGTACGAAAT 600 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 344 CTAAAGGTTTGGAGAAGAAATCTACCTTGGGAGCTTCACTTGTACGAAAT 393 Wildtype 601 GCTTCTTCGAGAATCCGGCAAGTGTCTCAAGAGCTCAAGCGATTGGCTTC 650 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 394 GCTTCTTCGAGAATCCGGCAAGTGTCTCAAGAGCTCAAGCGATTGGCTTC 443 Wildtype 651 CTTAAATAAGCGCCCAATTCCTACTGGAAGGTTCGACAGGAATAAATCAG 700 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 444 CTTAAATAAGCGCCCAATTCCTACTGGAAGGTTCGACAGGAATAAATCAG 493 Wildtype 701 CTGCTGCTCATGCTCTTAAAGGTCTTAAGTTC 732 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 494 CTGCTGCTCATGCTCTTAAAGGTCTTAAGTTC 525

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0018-5-4 (T1 )

CRT0018

CTG0018-2

CGR0018-2

gene : Solyc03g117980 (RbohB)

-7 bp /-7 bp

Is tDNA present: Yes

Mutation sequence:

GAACTTAAGACCTTTAAGAGCATGAGCAGCAGCTGATTTATTCCTGTCGAACCTTCCAGTAGGAATTGGGCGCTTATTTA
AGGAAGCCAATCGCTTGAGCTCTTGAGACACTTGCCGGATTCTCGAAGAAGCATTTCGTACAAGTGAAGCTCCCAAGGTA
GATTTCTTCTCCAAACCTTTAGCCAATAAAGCCAACTCCGGATCTTCCACGTCAGCACCGCCGGCACACTGTGGACAGCC
ACAGAGTCTTCACGAACATCGAGAGTGATTTCCACATAAGCATCGTCATTGGACTTGGCGCCGGCTCCTGCACTAGTTCC
GATGTCAGTAGATTCCGGTATATTAAACCTCGCGCTCTTTTTTCCTCCTCGTTTGTTCAGCGGTCCGCTTAAAGGACCAC
TGTACGACGCTCTATCATTCCCTATAACCTCCGTATCCGAGTGATGATGGTGATGGTGTGGGTGATGATTTTCCGAATTT
TGCATCGCTACAATAGTTTCTTCGTGGAAAAATGAAATGAATGAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         201 TTCATTCATTTCATTTTTCCACGAAGAAACTATTGTAGCGATGCAAAATT    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TTCATTCATTTCATTTTTCCACGAAGAAACTATTGTAGCGATGCAAAATT     50

Wildtype         251 CGGAAAATCATCACCCACACCATCACCATCATCACTCGGATACGGAGGTT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CGGAAAATCATCACCCACACCATCACCATCATCACTCGGATACGGAGGTT    100

Wildtype         301 ATAGGGAATGATAGAGCGTCGTACAGTGGTCCTTTAAGCGGACCGCTGAA    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATAGGGAATGATAGAGCGTCGTACAGTGGTCCTTTAAGCGGACCGCTGAA    150

Wildtype         351 CAAACGAGGAGGAAAAAAGAGCGCGAGGTTTAATATACCGGAATCTACTG    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 CAAACGAGGAGGAAAAAAGAGCGCGAGGTTTAATATACCGGAATCTACTG    200

Wildtype         401 ACATCGGAACTAGTGCAGGAGCCGGCGCCAAGTCCAATGACGATGCTTAT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 ACATCGGAACTAGTGCAGGAGCCGGCGCCAAGTCCAATGACGATGCTTAT    250

Wildtype         451 GTGGAAATCACTCTCGATGTTCGTGAAGACTCTGTGGCTGTCCACAGTGT    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||| 
MutationRev      251 GTGGAAATCACTCTCGATGTTCGTGAAGACTCTGTGGCTGTCCACAGTG-    299
gRNA                                                        GTCCACAGTGT

Wildtype         501 GAAAACTGCCGGCGGTGCTGACGTGGAAGATCCGGAGTTGGCTTTATTGG    550
                           ||||||||||||||||||||||||||||||||||||||||||||
MutationRev      300 ------TGCCGGCGGTGCTGACGTGGAAGATCCGGAGTTGGCTTTATTGG    343
gRNA                 GTCCACAGTGTGAAAACTGC

Wildtype         551 CTAAAGGTTTGGAGAAGAAATCTACCTTGGGAGCTTCACTTGTACGAAAT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      344 CTAAAGGTTTGGAGAAGAAATCTACCTTGGGAGCTTCACTTGTACGAAAT    393

Wildtype         601 GCTTCTTCGAGAATCCGGCAAGTGTCTCAAGAGCTCAAGCGATTGGCTTC    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      394 GCTTCTTCGAGAATCCGGCAAGTGTCTCAAGAGCTCAAGCGATTGGCTTC    443

Wildtype         651 CTTAAATAAGCGCCCAATTCCTACTGGAAGGTTCGACAGGAATAAATCAG    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      444 CTTAAATAAGCGCCCAATTCCTACTGGAAGGTTCGACAGGAATAAATCAG    493

Wildtype         701 CTGCTGCTCATGCTCTTAAAGGTCTTAAGTTC    732
                     ||||||||||||||||||||||||||||||||
MutationRev      494 CTGCTGCTCATGCTCTTAAAGGTCTTAAGTTC    525