Plant Crispr Database

Plant Information

Name: CRT0020-1-1

Seed stock: 5403
Seed availability: No

Relationship:
Parent: CRT0020-1
Progeny: CRT0020-1-1-7
Progeny: CRT0020-1-1-6
Progeny: CRT0020-1-1-3

Transformation: CRT0020
transformation CRT0020 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0020-1-1 (T1 )	CRT0020	CTG0020-1	CGR0020-1	gene : Solyc06g071810 (SOBIR)	-1 bp/-1 bp Is tDNA present: Yes Mutation sequence: CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGAGGGAATAACGATTCAAGTTTAACGATATAC AGTCCACTGATCAAGAAAGCCGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAATGATTGGAAA AGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCACCGGGGAGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATC CCCAATGGATGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGCGTCAAGTTAAATCAGAAATTC AAATTGTAGGTCAAATCAGACACCGGAATCTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGTC TACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCACAGAAGGCACAAGGGAATTAGATTGGTTGGG ACGACACAGAATTGCAGCGGGGGTAGCTGCTGGTCTCGAGTATCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 857 CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGA 906 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGA 50 Wildtype 907 GGGAATAACGATTCAAGTTTAACGATATACAGTCCACTGATCAAGAAAGC 956 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 GGGAATAACGATTCAAGTTTAACGATATACAGTCCACTGATCAAGAAAGC 100 Wildtype 957 CGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAA 1006 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 CGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAA 150 Wildtype 1007 TGATTGGAAAAGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCTACCGGGG 1056 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\| Mutation 151 TGATTGGAAAAGGTGGCTGTGGAGAAGTTTATAGAGCAGAGC-ACCGGGG 199 gRNA GTTTATAGAGCAGAGCTACC Wildtype 1057 AGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATCCCCAATGGA 1106 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 200 AGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATCCCCAATGGA 249 Wildtype 1107 TGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGC 1156 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 250 TGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGC 299 Wildtype 1157 GTCAAGTTAAATCAGAAATTCAAATTGTAGGTCAAATCAGACACCGGAAT 1206 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 300 GTCAAGTTAAATCAGAAATTCAAATTGTAGGTCAAATCAGACACCGGAAT 349 Wildtype 1207 CTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGT 1256 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 350 CTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGT 399 Wildtype 1257 CTACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCA 1306 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 400 CTACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCA 449 Wildtype 1307 CAGAAGGCACAAGGGAATTAGATTGGTTGGGACGACACAGAATTGCAGCG 1356 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 450 CAGAAGGCACAAGGGAATTAGATTGGTTGGGACGACACAGAATTGCAGCG 499 Wildtype 1357 GGGGTAGCTGCTGGTCTCGAGTATCTC 1383 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 500 GGGGTAGCTGCTGGTCTCGAGTATCTC 526

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0020-1-1 (T1 )

CRT0020

CTG0020-1

CGR0020-1

gene : Solyc06g071810 (SOBIR)

-1 bp/-1 bp

Is tDNA present: Yes

Mutation sequence:

CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGAGGGAATAACGATTCAAGTTTAACGATATAC
AGTCCACTGATCAAGAAAGCCGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAATGATTGGAAA
AGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCACCGGGGAGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATC
CCCAATGGATGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGCGTCAAGTTAAATCAGAAATTC
AAATTGTAGGTCAAATCAGACACCGGAATCTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGTC
TACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCACAGAAGGCACAAGGGAATTAGATTGGTTGGG
ACGACACAGAATTGCAGCGGGGGTAGCTGCTGGTCTCGAGTATCTC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         857 CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGA    906
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGA     50

Wildtype         907 GGGAATAACGATTCAAGTTTAACGATATACAGTCCACTGATCAAGAAAGC    956
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GGGAATAACGATTCAAGTTTAACGATATACAGTCCACTGATCAAGAAAGC    100

Wildtype         957 CGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAA   1006
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 CGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAA    150

Wildtype        1007 TGATTGGAAAAGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCTACCGGGG   1056
                     |||||||||||||||||||||||||||||||||||||||||| |||||||
Mutation         151 TGATTGGAAAAGGTGGCTGTGGAGAAGTTTATAGAGCAGAGC-ACCGGGG    199
gRNA                                           GTTTATAGAGCAGAGCTACC

Wildtype        1057 AGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATCCCCAATGGA   1106
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         200 AGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATCCCCAATGGA    249

Wildtype        1107 TGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGC   1156
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 TGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGC    299

Wildtype        1157 GTCAAGTTAAATCAGAAATTCAAATTGTAGGTCAAATCAGACACCGGAAT   1206
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 GTCAAGTTAAATCAGAAATTCAAATTGTAGGTCAAATCAGACACCGGAAT    349

Wildtype        1207 CTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGT   1256
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         350 CTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGT    399

Wildtype        1257 CTACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCA   1306
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         400 CTACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCA    449

Wildtype        1307 CAGAAGGCACAAGGGAATTAGATTGGTTGGGACGACACAGAATTGCAGCG   1356
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         450 CAGAAGGCACAAGGGAATTAGATTGGTTGGGACGACACAGAATTGCAGCG    499

Wildtype        1357 GGGGTAGCTGCTGGTCTCGAGTATCTC   1383
                     |||||||||||||||||||||||||||
Mutation         500 GGGGTAGCTGCTGGTCTCGAGTATCTC    526