Is tDNA present: Yes

Mutation sequence:

CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGAGGGAATAACGATTCAAGTTTAACGATATAC
AGTCCACTGATCAAGAAAGCCGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAATGATTGGAAA
AGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCACCGGGGAGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATC
CCCAATGGATGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGCGTCAAGTTAAATCAGAAATTC
AAATTGTAGGTCAAATCAGACACCGGAATCTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGTC
TACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCACAGAAGGCACAAGGGAATTAGATTGGTTGGG
ACGACACAGAATTGCAGCGGGGGTAGCTGCTGGTCTCGAGTATCTC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         857 CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGA    906
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGA     50

Wildtype         907 GGGAATAACGATTCAAGTTTAACGATATACAGTCCACTGATCAAGAAAGC    956
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GGGAATAACGATTCAAGTTTAACGATATACAGTCCACTGATCAAGAAAGC    100

Wildtype         957 CGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAA   1006
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 CGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAA    150

Wildtype        1007 TGATTGGAAAAGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCTACCGGGG   1056
                     |||||||||||||||||||||||||||||||||||||||||| |||||||
Mutation         151 TGATTGGAAAAGGTGGCTGTGGAGAAGTTTATAGAGCAGAGC-ACCGGGG    199
gRNA                                           GTTTATAGAGCAGAGCTACC

Wildtype        1057 AGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATCCCCAATGGA   1106
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         200 AGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATCCCCAATGGA    249

Wildtype        1107 TGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGC   1156
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 TGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGC    299

Wildtype        1157 GTCAAGTTAAATCAGAAATTCAAATTGTAGGTCAAATCAGACACCGGAAT   1206
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 GTCAAGTTAAATCAGAAATTCAAATTGTAGGTCAAATCAGACACCGGAAT    349

Wildtype        1207 CTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGT   1256
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         350 CTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGT    399

Wildtype        1257 CTACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCA   1306
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         400 CTACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCA    449

Wildtype        1307 CAGAAGGCACAAGGGAATTAGATTGGTTGGGACGACACAGAATTGCAGCG   1356
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         450 CAGAAGGCACAAGGGAATTAGATTGGTTGGGACGACACAGAATTGCAGCG    499

Wildtype        1357 GGGGTAGCTGCTGGTCTCGAGTATCTC   1383
                     |||||||||||||||||||||||||||
Mutation         500 GGGGTAGCTGCTGGTCTCGAGTATCTC    526