Plant Crispr Database

Plant Information

Name: CRT0022-4-8-4

Seed stock: 5602
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0022-4-8

Transformation: CRT0022
transformation CRT0022 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0022-4-8-4 (T2 )	CRT0022	CTG0022-2	CGR0022-2	gene : Solyc10g047320 (NRC2)	+1 bp /+1 bp Is tDNA present: No Mutation sequence: ATGCCTGGTCTCGGCAAAACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTAT TTGGGTTTATGTGTCTCAATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAAC AATATCATGGGATGTGTGAGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTG TTGGATGACGTATGGTCGGATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATTCGAGTAT TGTTGACCACTCGAGATTCCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAA AGTTGGATATTACTGGAGAAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1547 TTCCACTCTCTTGGTGTCTTGCCTTTCCCTATTAGTGCTCCTGCAATAAC 1596 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TTCCACTCTCTTGGTGTCTTGCCTTTCCCTATTAGTGCTCCTGCAATAAC 50 Wildtype 1597 AACAATCGCAAGTGGTAGTCCCTTGCATTTTTTTGCTATGCTCTTTCCAG 1646 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 AACAATCGCAAGTGGTAGTCCCTTGCATTTTTTTGCTATGCTCTTTCCAG 100 Wildtype 1647 ATAGTACCAATTCAGGAGGACATTTGTCCTTGTGAAAAACCTTCTTCTCC 1696 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 ATAGTACCAATTCAGGAGGACATTTGTCCTTGTGAAAAACCTTCTTCTCC 150 Wildtype 1697 AGTAATATCCAACTTTCATCTTCAGTCAGAAATTTTAGATCATGAGGTAT 1746 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 AGTAATATCCAACTTTCATCTTCAGTCAGAAATTTTAGATCATGAGGTAT 200 Wildtype 1747 GGGATTGCATTGTTTAGCCACTTTGGAATCTCGAGTGGTCAACAATACTC 1796 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GGGATTGCATTGTTTAGCCACTTTGGAATCTCGAGTGGTCAACAATACTC 250 gRNA GTGGTCAACAATACTC Wildtype 1797 G-ATTCGGTTTGTTGTTATTTGGGAATGCTATCTTGATACGTTCCCAAGC 1845 \| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GNATTCGGTTTGTTGTTATTTGGGAATGCTATCTTGATACGTTCCCAAGC 300 gRNA G-TGGTCAACAATACTCGATT Wildtype 1846 TTCATCCGACCATACGTCATCCAACACAACCAAGTATTTTCCTCCCTTTC 1895 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 TTCATCCGACCATACGTCATCCAACACAACCAAGTATTTTCCTCCCTTTC 350 Wildtype 1896 CCAAGAATTCTTGTATTTCATCAGCCAAATCCTCCTCACACATCCCATGA 1945 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 CCAAGAATTCTTGTATTTCATCAGCCAAATCCTCCTCACACATCCCATGA 400 Wildtype 1946 TATTGTTTTGTATTTCGAGTGAATTTGCTGATGATGTTGAGAAATAATTC 1995 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 TATTGTTTTGTATTTCGAGTGAATTTGCTGATGATGTTGAGAAATAATTC 450 Wildtype 1996 TCTTCTCCTGTATGATTGAGACACATAAACCCAAATACGAGTAAAAAACT 2045 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 TCTTCTCCTGTATGATTGAGACACATAAACCCAAATACGAGTAAAAAACT 500 Wildtype 2046 CATACCCGATTTTAGGATGCTTGTAAATCTTATTTGCTAGGGTCGT 2091 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 CATACCCGATTTTAGGATGCTTGTAAATCTTATTTGCTAGGGTCGT 546

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0022-4-8-4 (T2 )

CRT0022

CTG0022-2

CGR0022-2

gene : Solyc10g047320 (NRC2)

+1 bp /+1 bp

Is tDNA present: No

Mutation sequence:

ATGCCTGGTCTCGGCAAAACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTAT
TTGGGTTTATGTGTCTCAATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAAC
AATATCATGGGATGTGTGAGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTG
TTGGATGACGTATGGTCGGATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATTCGAGTAT
TGTTGACCACTCGAGATTCCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAA
AGTTGGATATTACTGGAGAAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1547 TTCCACTCTCTTGGTGTCTTGCCTTTCCCTATTAGTGCTCCTGCAATAAC   1596
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TTCCACTCTCTTGGTGTCTTGCCTTTCCCTATTAGTGCTCCTGCAATAAC     50

Wildtype        1597 AACAATCGCAAGTGGTAGTCCCTTGCATTTTTTTGCTATGCTCTTTCCAG   1646
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 AACAATCGCAAGTGGTAGTCCCTTGCATTTTTTTGCTATGCTCTTTCCAG    100

Wildtype        1647 ATAGTACCAATTCAGGAGGACATTTGTCCTTGTGAAAAACCTTCTTCTCC   1696
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATAGTACCAATTCAGGAGGACATTTGTCCTTGTGAAAAACCTTCTTCTCC    150

Wildtype        1697 AGTAATATCCAACTTTCATCTTCAGTCAGAAATTTTAGATCATGAGGTAT   1746
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 AGTAATATCCAACTTTCATCTTCAGTCAGAAATTTTAGATCATGAGGTAT    200

Wildtype        1747 GGGATTGCATTGTTTAGCCACTTTGGAATCTCGAGTGGTCAACAATACTC   1796
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GGGATTGCATTGTTTAGCCACTTTGGAATCTCGAGTGGTCAACAATACTC    250
gRNA                                                   GTGGTCAACAATACTC

Wildtype        1797 G-ATTCGGTTTGTTGTTATTTGGGAATGCTATCTTGATACGTTCCCAAGC   1845
                     | ||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 GNATTCGGTTTGTTGTTATTTGGGAATGCTATCTTGATACGTTCCCAAGC    300
gRNA                 G-TGGTCAACAATACTCGATT

Wildtype        1846 TTCATCCGACCATACGTCATCCAACACAACCAAGTATTTTCCTCCCTTTC   1895
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 TTCATCCGACCATACGTCATCCAACACAACCAAGTATTTTCCTCCCTTTC    350

Wildtype        1896 CCAAGAATTCTTGTATTTCATCAGCCAAATCCTCCTCACACATCCCATGA   1945
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 CCAAGAATTCTTGTATTTCATCAGCCAAATCCTCCTCACACATCCCATGA    400

Wildtype        1946 TATTGTTTTGTATTTCGAGTGAATTTGCTGATGATGTTGAGAAATAATTC   1995
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      401 TATTGTTTTGTATTTCGAGTGAATTTGCTGATGATGTTGAGAAATAATTC    450

Wildtype        1996 TCTTCTCCTGTATGATTGAGACACATAAACCCAAATACGAGTAAAAAACT   2045
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 TCTTCTCCTGTATGATTGAGACACATAAACCCAAATACGAGTAAAAAACT    500

Wildtype        2046 CATACCCGATTTTAGGATGCTTGTAAATCTTATTTGCTAGGGTCGT   2091
                     ||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      501 CATACCCGATTTTAGGATGCTTGTAAATCTTATTTGCTAGGGTCGT    546