Plant Crispr Database

Plant Information

Name: CRT0022-5

Seed stock:
Seed availability: No

Relationship:
Progeny: CRT0022-5-2

Transformation: CRT0022
transformation CRT0022 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0022-5 (T0 )	CRT0022	CTG0022-3	CGR0022-3	gene : Solyc05g009630 (NRC3)	-12 bp/-12 bp Is tDNA present: Yes Mutation sequence: TTCTTATTAGAAAATTTGACGCAGTTACTAATCGACAACGCCGATTTCCAAGGCGAAGTTGAAAATCTACTCACAGATCT CAATTACTTTAATGCTTTCCTCAAAGAAGCTGCTAAATCCCGCAGGGAAAATGAGGTTTGCCTTAAATTTAGGAATAATT TCATTTGTTTGCCATTCAAAATATGAACTGATTTTTTAAATATTATTGTTCGTTTCATA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 4358 TATGAAACGAACAATAATATTTAAAAAATCAGTTCATATTTTGAATGGCA 4407 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TATGAAACGAACAATAATATTTAAAAAATCAGTTCATATTTTGAATGGCA 50 Wildtype 4408 AACAAATGAAATTATTCCTAAATTTAAGGCAAACCTCATTTTCCCTGCGG 4457 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 AACAAATGAAATTATTCCTAAATTTAAGGCAAACCTCATTTTCCCTGCGG 100 Wildtype 4458 GATTTAGCAGCTTCTTTGAGGAAAGCATTAAAGTAATTGAGATCTGTGAG 4507 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 GATTTAGCAGCTTCTTTGAGGAAAGCATTAAAGTAATTGAGATCTGTGAG 150 Wildtype 4508 TAGATTTTCAACTTCGCCTTGGATTCCGAGAATCAAATCGGCGTTGTCGA 4557 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TAGATTTTCAACTTCGCCTTGG------------AAATCGGCGTTGTCGA 188 gRNA TGGATTCCGAGAATCAAATC Wildtype 4558 TTAGTAACTGCGTCAAATTTTCTAATAAGAA 4588 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 189 TTAGTAACTGCGTCAAATTTTCTAATAAGAA 219

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0022-5 (T0 )

CRT0022

CTG0022-3

CGR0022-3

gene : Solyc05g009630 (NRC3)

-12 bp/-12 bp

Is tDNA present: Yes

Mutation sequence:

TTCTTATTAGAAAATTTGACGCAGTTACTAATCGACAACGCCGATTTCCAAGGCGAAGTTGAAAATCTACTCACAGATCT
CAATTACTTTAATGCTTTCCTCAAAGAAGCTGCTAAATCCCGCAGGGAAAATGAGGTTTGCCTTAAATTTAGGAATAATT
TCATTTGTTTGCCATTCAAAATATGAACTGATTTTTTAAATATTATTGTTCGTTTCATA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        4358 TATGAAACGAACAATAATATTTAAAAAATCAGTTCATATTTTGAATGGCA   4407
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TATGAAACGAACAATAATATTTAAAAAATCAGTTCATATTTTGAATGGCA     50

Wildtype        4408 AACAAATGAAATTATTCCTAAATTTAAGGCAAACCTCATTTTCCCTGCGG   4457
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 AACAAATGAAATTATTCCTAAATTTAAGGCAAACCTCATTTTCCCTGCGG    100

Wildtype        4458 GATTTAGCAGCTTCTTTGAGGAAAGCATTAAAGTAATTGAGATCTGTGAG   4507
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 GATTTAGCAGCTTCTTTGAGGAAAGCATTAAAGTAATTGAGATCTGTGAG    150

Wildtype        4508 TAGATTTTCAACTTCGCCTTGGATTCCGAGAATCAAATCGGCGTTGTCGA   4557
                     ||||||||||||||||||||||            ||||||||||||||||
MutationRev      151 TAGATTTTCAACTTCGCCTTGG------------AAATCGGCGTTGTCGA    188
gRNA                                    TGGATTCCGAGAATCAAATC

Wildtype        4558 TTAGTAACTGCGTCAAATTTTCTAATAAGAA   4588
                     |||||||||||||||||||||||||||||||
MutationRev      189 TTAGTAACTGCGTCAAATTTTCTAATAAGAA    219