Is tDNA present: Yes

Mutation sequence:

CTCAAGCAAGCTGCAATGGTCCGCAGCGAAAACCCAGTTCTCAAAGAACTAGTGAGGAGTATCAGAAAAGTGGTGAATCG
TGCTGAAGATGCTGTTGATAAATTTGTAATTGAAGCTAAAGTTCATAAAGACAAAGGGTTTAAAGGGGTTTTCGATAAAC
CTGGACATTATAGAAGAGTGAGGGATGCAGCTGTGGAGATTAAAGGTATCAGAGATAAAATGAGAGAAATTCGGCAAAAT
AAGGCACATGGCCTTCAGGCTCTACTTCAAGATCATGATGATTCAATCAGCAGGTGGAGAAGAGAGACAGGTATTGCTTC
AAGTCGTTAATTTCGACAAAATTAGCTCATGAAAATATTAGGATCTGGTCAACACACCTATATTTGTGTGGGTTTATATA
TTAATTTGATCCACCTAATTCAGATCGTGTAAAAGTTTGAAACCAAATTAACCATGAGACAGGAAGATAAAAGTGAACGA
TATATTTATATGAGAACGAATTTTAGCCAACATATCCACAAAATGAGTATTTTATTTGGATTTGAATGAAACATTTGAAT
TATTAAGATATTGTTTCTGCATTTAAACACATGCATAATTTATTCATTTGTAGAGATAAAGAAATTTATAATTCAAATGA
AATAAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         187 TTCAATGCCTTTCTCAAGCAAGCTGCAATGGTCCGCAGCGAAAACCCAGT    236
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TTCAATGCCTTTCTCAAGCAAGCTGCAATGGTCCGCAGCGAAAACCCAGT     50

Wildtype         237 TCTCAAAGAACTAGTGAGGAGTATCAGAAAAGTGGTGAATCGTGCTGAAG    286
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TCTCAAAGAACTAGTGAGGAGTATCAGAAAAGTGGTGAATCGTGCTGAAG    100

Wildtype         287 ATGCTGTTGATAAATTTGTAATTGAAGCTAAAGTTCATAAAGACAAAGGG    336
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATGCTGTTGATAAATTTGTAATTGAAGCTAAAGTTCATAAAGACAAAGGG    150

Wildtype         337 TTTAAAGGGGTTTTCGATAAACCTGGACATTATAGAAGAGTGAGGGATGC    386
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TTTAAAGGGGTTTTCGATAAACCTGGACATTATAGAAGAGTGAGGGATGC    200

Wildtype         387 AGCTGTGGAGATTAAAGGTATCAGAGATAAAATGAGAGAAATTCGGCAAA    436
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 AGCTGTGGAGATTAAAGGTATCAGAGATAAAATGAGAGAAATTCGGCAAA    250

Wildtype         437 ATAAGGCACATGGCCTTCAGGCTCTACTTCAAGATCATGATGATTCAATC    486
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 ATAAGGCACATGGCCTTCAGGCTCTACTTCAAGATCATGATGATTCAATC    300
gRNA                                                       GATGATTCAATC

Wildtype         487 AGCAGAGGTGGAGAAGAGAGACAGGTATTGCTTCAAGTCGTTAATTTCGA    536
                          |||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 -----AGGTGGAGAAGAGAGACAGGTATTGCTTCAAGTCGTTAATTTCGA    345
gRNA                 GATGATTCAATCAGCAGAGG

Wildtype         537 CAAAATTAGCTCATGAAAATATTAGGATCTGGTCAACACACCTATATTTG    586
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         346 CAAAATTAGCTCATGAAAATATTAGGATCTGGTCAACACACCTATATTTG    395

Wildtype         587 TGTGGGTTTATATATTAATTTGATCCACCTAATTCAGATCGTGTAAAAGT    636
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         396 TGTGGGTTTATATATTAATTTGATCCACCTAATTCAGATCGTGTAAAAGT    445

Wildtype         637 TTGAAACCAAATTAACCATGAGACAGGAAGATAAAAGTGAACGATATATT    686
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         446 TTGAAACCAAATTAACCATGAGACAGGAAGATAAAAGTGAACGATATATT    495

Wildtype         687 TATATGAGAACGAATTTTAGCCAACATATCCACAAAATGAGTATTTTATT    736
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         496 TATATGAGAACGAATTTTAGCCAACATATCCACAAAATGAGTATTTTATT    545

Wildtype         737 TGGATTTGAATGAAACATTTGAATTATTAAGATATTGTTTCTGCATTTAA    786
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         546 TGGATTTGAATGAAACATTTGAATTATTAAGATATTGTTTCTGCATTTAA    595

Wildtype         787 ACACATGCATAATTTATTCATTTGTAGAGATAAAGAAATTTATAATTCAA    836
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         596 ACACATGCATAATTTATTCATTTGTAGAGATAAAGAAATTTATAATTCAA    645

Wildtype         837 ATGAA    841
                     |||||
Mutation         646 ATGAA    650