Plant Crispr Database

Plant Information

Name: CRT0023-1-5

Seed stock: 5297
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0023-1

Transformation: CRT0023
transformation CRT0023 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0023-1-5 (T1 )	CRT0023	CTG0023-2	CGR0023-2	gene : Solyc04g049090 (SlMlo1)	-7 bp/-7 bp Is tDNA present: No Mutation sequence: CTCGCTATTTCTATTTTTATTGAACAAATTATTCATCACATTGGAGAGGTACCTACTATATAATTTCAAAATCTTTTACT AGATCGATATGAACAAAATATGCTTATTAATTGTTGGGAAAACCATTTTCTTTTTTAGTGGTTACTGGAAAAGCGGAAAA AGTCTCTATATGAAGCACTTGAAAAGCTGGTAAATTCAAAATACAGTCTTGTATTATAAAATAATATAGTTTAAAGATGG ATCATACTCATGACATTTTTGGTGTGTGTGTGTCGGACAGAACTTATGCTGTTGGGATTCTTATCACTGTTGTTGACAGT GTTGCAAGATCCAGTTTCTAACTTATGTGTCCCCAAGAGTGTTGGTTATTCATGGCATCCTTGTATGGCAAAGGAAGATG CCAAGTCTGAGTAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 4844 TACTCAGACTTGGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACC 4893 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 3 TACTCAGACTTGGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACC 52 Wildtype 4894 AACACTCTTGGGGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCA 4943 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 53 AACACTCTTGGGGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCA 102 Wildtype 4944 ACAACAGTGATAAGAATCCCAACAGCATAAGTTCTGTCCGACACACACAC 4993 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 103 ACAACAGTGATAAGAATCCCAACAGCATAAGTTCTGTCCGACACACACAC 152 Wildtype 4994 ACCAAAAATGTCATGAGTATGATCCATCTTTAAACTATATTATTTTATAA 5043 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 153 ACCAAAAATGTCATGAGTATGATCCATCTTTAAACTATATTATTTTATAA 202 Wildtype 5044 TACAAGACTGTATTTTGAATTTACCAGCTTTGATCTTTTCAAGTGCTTCA 5093 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 203 TACAAGACTGTATTTTGAATTTACCAG-------CTTTTCAAGTGCTTCA 245 gRNA GCTTTGATCTTTTCAAGTGC Wildtype 5094 TATAGAGACTTTTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTT 5143 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 246 TATAGAGACTTTTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTT 295 Wildtype 5144 TTCCCAACAATTAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGAT 5193 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 296 TTCCCAACAATTAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGAT 345 Wildtype 5194 TTTGAAATTATATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCA 5243 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 346 TTTGAAATTATATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCA 395 Wildtype 5244 ATAAAAATAGAAATAGCGAG 5263 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 396 ATAAAAATAGAAATAGCGAG 415

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0023-1-5 (T1 )

CRT0023

CTG0023-2

CGR0023-2

gene : Solyc04g049090 (SlMlo1)

-7 bp/-7 bp

Is tDNA present: No

Mutation sequence:

CTCGCTATTTCTATTTTTATTGAACAAATTATTCATCACATTGGAGAGGTACCTACTATATAATTTCAAAATCTTTTACT
AGATCGATATGAACAAAATATGCTTATTAATTGTTGGGAAAACCATTTTCTTTTTTAGTGGTTACTGGAAAAGCGGAAAA
AGTCTCTATATGAAGCACTTGAAAAGCTGGTAAATTCAAAATACAGTCTTGTATTATAAAATAATATAGTTTAAAGATGG
ATCATACTCATGACATTTTTGGTGTGTGTGTGTCGGACAGAACTTATGCTGTTGGGATTCTTATCACTGTTGTTGACAGT
GTTGCAAGATCCAGTTTCTAACTTATGTGTCCCCAAGAGTGTTGGTTATTCATGGCATCCTTGTATGGCAAAGGAAGATG
CCAAGTCTGAGTAAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        4844 TACTCAGACTTGGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACC   4893
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        3 TACTCAGACTTGGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACC     52

Wildtype        4894 AACACTCTTGGGGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCA   4943
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       53 AACACTCTTGGGGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCA    102

Wildtype        4944 ACAACAGTGATAAGAATCCCAACAGCATAAGTTCTGTCCGACACACACAC   4993
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      103 ACAACAGTGATAAGAATCCCAACAGCATAAGTTCTGTCCGACACACACAC    152

Wildtype        4994 ACCAAAAATGTCATGAGTATGATCCATCTTTAAACTATATTATTTTATAA   5043
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      153 ACCAAAAATGTCATGAGTATGATCCATCTTTAAACTATATTATTTTATAA    202

Wildtype        5044 TACAAGACTGTATTTTGAATTTACCAGCTTTGATCTTTTCAAGTGCTTCA   5093
                     |||||||||||||||||||||||||||       ||||||||||||||||
MutationRev      203 TACAAGACTGTATTTTGAATTTACCAG-------CTTTTCAAGTGCTTCA    245
gRNA                                           GCTTTGATCTTTTCAAGTGC

Wildtype        5094 TATAGAGACTTTTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTT   5143
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      246 TATAGAGACTTTTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTT    295

Wildtype        5144 TTCCCAACAATTAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGAT   5193
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      296 TTCCCAACAATTAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGAT    345

Wildtype        5194 TTTGAAATTATATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCA   5243
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      346 TTTGAAATTATATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCA    395

Wildtype        5244 ATAAAAATAGAAATAGCGAG   5263
                     ||||||||||||||||||||
MutationRev      396 ATAAAAATAGAAATAGCGAG    415