Plant Crispr Database

Plant Information

Name: CRT0025-3-2

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0025-3

Transformation: CRT0025
transformation CRT0025 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0025-3-2 (T1 )	CRT0025	CTG0025-2	CGR0025-2	gene : Solyc07g049180 (Bti9)	-3 bp/-3 bp Is tDNA present: Yes Mutation sequence: GTTGGCTTATTTGTTATCCGTTTTCAGACCATTCATGATGATAAAGGAAGTCCTTTATGCTATCTACACTTACCATCATT GCATCCTTCTTAACAATTAGGTTTGTCTGGTGGCGCTAAAGCTGGGATATCTATAGGAGCAATAGGAGTAGTCTTGCTGG CTGGTTTGGTTTATGTAGGATGTTATAGGAACAAAACACGAAAGATTTCGCTGCTCAGATCGGAAGACCACCTCCATCAG TATGGTCATGGTAGGTGTTGATCCCTGTTCTTTAGTTCTTTAAGTTTATAACATTCATTTATTTGGCGTACGACATTTCT TAAGGTGCTTATACTTGGATCACTTTAAGGCTGAAAATGCAATTTAAAGCAGCTGATTTCTTTGTTGGAATTGAATATTT CCTTTAGTTATGCTGAGTAGTAGAAAACAATGTTTGTTAGTTGACTTACATGTTAAGCTTTTGTCTTGATTAGGCCCAGA AGGCAGTACGACAGTTAAAGCTGCTGATTCCGGTCGCCTGGCTGATGGCAATTCTCCAGTGCTTTCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 3518 TGAAAGCACTGGAGAATTGCCATCAGCCAGGCGACCGGAATCAGCAGCTT 3567 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TGAAAGCACTGGAGAATTGCCATCAGCCAGGCGACCGGAATCAGCAGCTT 50 Wildtype 3568 TAACTGTCGTACTGCCTTCTGGGCCTAATCAAGACAAAAGCTTAACATGT 3617 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TAACTGTCGTACTGCCTTCTGGGCCTAATCAAGACAAAAGCTTAACATGT 100 Wildtype 3618 AAGTCAACTAACAAACATTGTTTTCTACTACTCAGCATAACTAAAGGAAA 3667 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AAGTCAACTAACAAACATTGTTTTCTACTACTCAGCATAACTAAAGGAAA 150 Wildtype 3668 TATTCAATTCCAACAAAGAAATCAGCTGCTTTAAATTGCATTTTCAGCCT 3717 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TATTCAATTCCAACAAAGAAATCAGCTGCTTTAAATTGCATTTTCAGCCT 200 Wildtype 3718 TAAAGTGATCCAAGTATAAGCACCTTAAGAAATGTCGTACGCCAAATAAA 3767 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TAAAGTGATCCAAGTATAAGCACCTTAAGAAATGTCGTACGCCAAATAAA 250 Wildtype 3768 TGAATGTTATAAACTTAAAGAACTAAAGAACAGGGATCAACACCTACCAT 3817 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TGAATGTTATAAACTTAAAGAACTAAAGAACAGGGATCAACACCTACCAT 300 Wildtype 3818 GACCATACTGATGGAGGTGGTCTTCCGATCTGAGCAGCGAAATCTTTCGT 3867 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 GACCATACTGATGGAGGTGGTCTTCCGATCTGAGCAGCGAAATCTTTCGT 350 Wildtype 3868 GTTTTGTTCCTATAACATCCTACATAAACCAAACCAGCCAACAGCAAGAC 3917 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\| MutationRev 351 GTTTTGTTCCTATAACATCCTACATAAACCAAACCAGC---CAGCAAGAC 397 gRNA GCCAACAGCAAGAC Wildtype 3918 TACTCCTATTGCTCCTATAGATATCCCAGCTTTAGCGCCACCAGACAAAC 3967 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 398 TACTCCTATTGCTCCTATAGATATCCCAGCTTTAGCGCCACCAGACAAAC 447 gRNA GCCAACAGCAAGACTACTCC Wildtype 3968 CTAATTGTTAAGAAGGATGCAATGATGGTAAGTGTAGATAGCATAAAGGA 4017 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 448 CTAATTGTTAAGAAGGATGCAATGATGGTAAGTGTAGATAGCATAAAGGA 497 Wildtype 4018 CTTCCTTTATCATCATGAATGGTCTGAAAACGGATAACAAATAAGCCAAC 4067 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 498 CTTCCTTTATCATCATGAATGGTCTGAAAACGGATAACAAATAAGCCAAC 547

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0025-3-2 (T1 )

CRT0025

CTG0025-2

CGR0025-2

gene : Solyc07g049180 (Bti9)

-3 bp/-3 bp

Is tDNA present: Yes

Mutation sequence:

GTTGGCTTATTTGTTATCCGTTTTCAGACCATTCATGATGATAAAGGAAGTCCTTTATGCTATCTACACTTACCATCATT
GCATCCTTCTTAACAATTAGGTTTGTCTGGTGGCGCTAAAGCTGGGATATCTATAGGAGCAATAGGAGTAGTCTTGCTGG
CTGGTTTGGTTTATGTAGGATGTTATAGGAACAAAACACGAAAGATTTCGCTGCTCAGATCGGAAGACCACCTCCATCAG
TATGGTCATGGTAGGTGTTGATCCCTGTTCTTTAGTTCTTTAAGTTTATAACATTCATTTATTTGGCGTACGACATTTCT
TAAGGTGCTTATACTTGGATCACTTTAAGGCTGAAAATGCAATTTAAAGCAGCTGATTTCTTTGTTGGAATTGAATATTT
CCTTTAGTTATGCTGAGTAGTAGAAAACAATGTTTGTTAGTTGACTTACATGTTAAGCTTTTGTCTTGATTAGGCCCAGA
AGGCAGTACGACAGTTAAAGCTGCTGATTCCGGTCGCCTGGCTGATGGCAATTCTCCAGTGCTTTCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        3518 TGAAAGCACTGGAGAATTGCCATCAGCCAGGCGACCGGAATCAGCAGCTT   3567
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TGAAAGCACTGGAGAATTGCCATCAGCCAGGCGACCGGAATCAGCAGCTT     50

Wildtype        3568 TAACTGTCGTACTGCCTTCTGGGCCTAATCAAGACAAAAGCTTAACATGT   3617
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TAACTGTCGTACTGCCTTCTGGGCCTAATCAAGACAAAAGCTTAACATGT    100

Wildtype        3618 AAGTCAACTAACAAACATTGTTTTCTACTACTCAGCATAACTAAAGGAAA   3667
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AAGTCAACTAACAAACATTGTTTTCTACTACTCAGCATAACTAAAGGAAA    150

Wildtype        3668 TATTCAATTCCAACAAAGAAATCAGCTGCTTTAAATTGCATTTTCAGCCT   3717
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 TATTCAATTCCAACAAAGAAATCAGCTGCTTTAAATTGCATTTTCAGCCT    200

Wildtype        3718 TAAAGTGATCCAAGTATAAGCACCTTAAGAAATGTCGTACGCCAAATAAA   3767
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 TAAAGTGATCCAAGTATAAGCACCTTAAGAAATGTCGTACGCCAAATAAA    250

Wildtype        3768 TGAATGTTATAAACTTAAAGAACTAAAGAACAGGGATCAACACCTACCAT   3817
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 TGAATGTTATAAACTTAAAGAACTAAAGAACAGGGATCAACACCTACCAT    300

Wildtype        3818 GACCATACTGATGGAGGTGGTCTTCCGATCTGAGCAGCGAAATCTTTCGT   3867
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 GACCATACTGATGGAGGTGGTCTTCCGATCTGAGCAGCGAAATCTTTCGT    350

Wildtype        3868 GTTTTGTTCCTATAACATCCTACATAAACCAAACCAGCCAACAGCAAGAC   3917
                     ||||||||||||||||||||||||||||||||||||||   |||||||||
MutationRev      351 GTTTTGTTCCTATAACATCCTACATAAACCAAACCAGC---CAGCAAGAC    397
gRNA                                                     GCCAACAGCAAGAC

Wildtype        3918 TACTCCTATTGCTCCTATAGATATCCCAGCTTTAGCGCCACCAGACAAAC   3967
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      398 TACTCCTATTGCTCCTATAGATATCCCAGCTTTAGCGCCACCAGACAAAC    447
gRNA                 GCCAACAGCAAGACTACTCC

Wildtype        3968 CTAATTGTTAAGAAGGATGCAATGATGGTAAGTGTAGATAGCATAAAGGA   4017
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      448 CTAATTGTTAAGAAGGATGCAATGATGGTAAGTGTAGATAGCATAAAGGA    497

Wildtype        4018 CTTCCTTTATCATCATGAATGGTCTGAAAACGGATAACAAATAAGCCAAC   4067
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      498 CTTCCTTTATCATCATGAATGGTCTGAAAACGGATAACAAATAAGCCAAC    547