Plant Crispr Database

Plant Information

Name: CRT0029-4-4

Seed stock: 5341
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0029-4

Transformation: CRT0029
transformation CRT0029 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0029-4-4 (T1 )	CRT0029	CTG0029-2	CGR0029-2	gene : Solyc01g099520 (RALF2)	+44 bp and nucleotide substitution Is tDNA present: No Mutation sequence: ACAGCCCCATCTCCAAAGAAAAAAAAATAAAATTAAAAATTCAATATACCAAGAGAAATACAATTTTTTTTTATGGATCC TGTGGAAGAAGAAGAGAAAATAGAGAACCTAGCTCCTGCAACGAGTAATAGCACTGCATCCATCGGTTAAAATGTGAAAT TGTTTAGCTACAAGAGGAGTCACAAATGACAAGGTAGAACCGGTATCATGTAAGTCTTATACATCAACAGAGAAGGCCCA CGGCGAGAACACGGCACACTGTTCTTCTGGAGTGCACCATAGCTGATGTACTTTTTGGTTGCTAAAATACGCCTGTTTGA CTCACTGTCAAGGGCAAACTCATCTTCTTCAGCCATGCACTCTGCAATACTCCCCTTGCATTCACCGGATCTCGCCGGCA CCATCCAATCGTAGCTACCACTGTCGCCGGCGGCAGCCATGGAAATGAAAAAAGCTCCAACAAGAACACAAACAATCAAA TACGAAGAAACTCCCATTTTTTTTTCCTTGAATTGAAAAAAAAATGAAAGAGAANTTAANGCANCCTTTAGCTAATTCTT CNGGTTTATGATTCGGACGGCTAGTTTTGCCGTATTTTCNATGTTGAAAATTTGTTAGTTTCTGCAAATTGNGAANAANA CAAGTTATAANGGGCAAGGCCAGTTGTATTATAAGTAGAGGNGGTTGTTTATTTACCCCTAATACTTTACTTTAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 221 TATGGATCCTGTGGAAGAAGAAGAGAAAATAGAGAACCTAGCTCCTGCAA 270 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TATGGATCCTGTGGAAGAAGAAGAGAAAATAGAGAACCTAGCTCCTGCAA 50 Wildtype 271 CGAGTAATAGCACTGCATCCACGAGTGTAGGGATTTGCTTGAGCTCCAGG 320 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CGAGTAATAGCACTGCATCCACGAGTGTAGGGATTTGCTTGAGCTCCAGG 100 Wildtype 321 TTTGCAGTTGTAGTAGGAAGCTCCACGGCGAGAACACGGCACACTGTTCT 370 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TTTGCAGTTGTAGTAG-----TCCACGGCGAGAACACGGCACACTGTTCT 145 gRNA GTTGTAGTAGGAAGCTCCA Wildtype 371 TCTGGAGTGCACCATAGCTGATGTACTTTTTGGTTGCTAAAATACGCCTG 420 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 146 TCTGGAGTGCACCATAGCTGATGTACTTTTTGGTTGCTAAAATACGCCTG 195 Wildtype 421 TTTGACTCACTGTCAAGGGCAAACTCATCTTCTTCAGCCATGCACTCTGC 470 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 196 TTTGACTCACTGTCAAGGGCAAACTCATCTTCTTCAGCCATGCACTCTGC 245 Wildtype 471 AATACTCCCCTTGCATTCACCGGATCTCGCCGGCACCATCCAATCGTAGC 520 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 246 AATACTCCCCTTGCATTCACCGGATCTCGCCGGCACCATCCAATCGTAGC 295 Wildtype 521 TACCACTGTCGCCGGCGGCAGCCATGGAAATGAAAAAAGCTCCAACAAGA 570 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 296 TACCACTGTCGCCGGCGGCAGCCATGGAAATGAAAAAAGCTCCAACAAGA 345 Wildtype 571 ACACAAACAATCAAATACGAAGAAACTCCCATTTTTTTTTCCTTGAATTG 620 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 346 ACACAAACAATCAAATACGAAGAAACTCCCATTTTTTTTTCCTTGAATTG 395 Wildtype 621 AAAAAAAAATGAAAGAGA 638 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 396 AAAAAAAAATGAAAGAGA 413

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0029-4-4 (T1 )

CRT0029

CTG0029-2

CGR0029-2

gene : Solyc01g099520 (RALF2)

+44 bp and nucleotide substitution

Is tDNA present: No

Mutation sequence:

ACAGCCCCATCTCCAAAGAAAAAAAAATAAAATTAAAAATTCAATATACCAAGAGAAATACAATTTTTTTTTATGGATCC
TGTGGAAGAAGAAGAGAAAATAGAGAACCTAGCTCCTGCAACGAGTAATAGCACTGCATCCATCGGTTAAAATGTGAAAT
TGTTTAGCTACAAGAGGAGTCACAAATGACAAGGTAGAACCGGTATCATGTAAGTCTTATACATCAACAGAGAAGGCCCA
CGGCGAGAACACGGCACACTGTTCTTCTGGAGTGCACCATAGCTGATGTACTTTTTGGTTGCTAAAATACGCCTGTTTGA
CTCACTGTCAAGGGCAAACTCATCTTCTTCAGCCATGCACTCTGCAATACTCCCCTTGCATTCACCGGATCTCGCCGGCA
CCATCCAATCGTAGCTACCACTGTCGCCGGCGGCAGCCATGGAAATGAAAAAAGCTCCAACAAGAACACAAACAATCAAA
TACGAAGAAACTCCCATTTTTTTTTCCTTGAATTGAAAAAAAAATGAAAGAGAANTTAANGCANCCTTTAGCTAATTCTT
CNGGTTTATGATTCGGACGGCTAGTTTTGCCGTATTTTCNATGTTGAAAATTTGTTAGTTTCTGCAAATTGNGAANAANA
CAAGTTATAANGGGCAAGGCCAGTTGTATTATAAGTAGAGGNGGTTGTTTATTTACCCCTAATACTTTACTTTAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         221 TATGGATCCTGTGGAAGAAGAAGAGAAAATAGAGAACCTAGCTCCTGCAA    270
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TATGGATCCTGTGGAAGAAGAAGAGAAAATAGAGAACCTAGCTCCTGCAA     50

Wildtype         271 CGAGTAATAGCACTGCATCCACGAGTGTAGGGATTTGCTTGAGCTCCAGG    320
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CGAGTAATAGCACTGCATCCACGAGTGTAGGGATTTGCTTGAGCTCCAGG    100

Wildtype         321 TTTGCAGTTGTAGTAGGAAGCTCCACGGCGAGAACACGGCACACTGTTCT    370
                     ||||||||||||||||     |||||||||||||||||||||||||||||
MutationRev      101 TTTGCAGTTGTAGTAG-----TCCACGGCGAGAACACGGCACACTGTTCT    145
gRNA                       GTTGTAGTAGGAAGCTCCA

Wildtype         371 TCTGGAGTGCACCATAGCTGATGTACTTTTTGGTTGCTAAAATACGCCTG    420
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      146 TCTGGAGTGCACCATAGCTGATGTACTTTTTGGTTGCTAAAATACGCCTG    195

Wildtype         421 TTTGACTCACTGTCAAGGGCAAACTCATCTTCTTCAGCCATGCACTCTGC    470
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      196 TTTGACTCACTGTCAAGGGCAAACTCATCTTCTTCAGCCATGCACTCTGC    245

Wildtype         471 AATACTCCCCTTGCATTCACCGGATCTCGCCGGCACCATCCAATCGTAGC    520
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      246 AATACTCCCCTTGCATTCACCGGATCTCGCCGGCACCATCCAATCGTAGC    295

Wildtype         521 TACCACTGTCGCCGGCGGCAGCCATGGAAATGAAAAAAGCTCCAACAAGA    570
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      296 TACCACTGTCGCCGGCGGCAGCCATGGAAATGAAAAAAGCTCCAACAAGA    345

Wildtype         571 ACACAAACAATCAAATACGAAGAAACTCCCATTTTTTTTTCCTTGAATTG    620
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      346 ACACAAACAATCAAATACGAAGAAACTCCCATTTTTTTTTCCTTGAATTG    395

Wildtype         621 AAAAAAAAATGAAAGAGA    638
                     ||||||||||||||||||
MutationRev      396 AAAAAAAAATGAAAGAGA    413