Is tDNA present: Yes

Mutation sequence:

ATTCTCACTTTTATGCTATGAAGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATACTACCTTAT
GTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAACAGACAAAATGTCACTGCTAGCATTCAAGAATATGATCAT
TGATGACCCTTTCAAGATCATGGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCTGGGGTCTCCATGTGGTAAC
CGTCATTGCCGAGTTACAGTTTTAAATCTTACTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAG
CTTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCCATCAGAGATTGGCTACTTACATAAGTTAA
ACGTTTTACGCCTTGATAATAACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTTGTTTCTGTT
GGTCTTTCGTATAATATGATGGTAGGCGAAATTCCAGCAGAATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGT
TTCTAACTCTTTGACAGGAGGAATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACCTTTTCTGCAAGCAAGAATA
ATTTGTTAGGGAAAATACCTGATGAATTATGTCAATTACTCAACTTAAAGTACTTTGTAGTGAATGAAAACAACTTGAGT
AGTACCCTGCCTCCTTGTCTTTTCAATCTTTCCTCTATCGTGGCCATCGATGTTGGAACAAACCATTTAGAAGGACAATT
GCCTCCATTGCTTGGTATTACTCTTCCTAAGTTAGAGTTCCTTAGCATTTATAGAAACAATGTAAC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype          38 AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATA     87
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATA     50

Wildtype          88 CTACCTTATGTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAAC    137
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CTACCTTATGTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAAC    100

Wildtype         138 AGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTT    187
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 AGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTT    150

Wildtype         188 TCAAGATCATGGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCT    237
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCAAGATCATGGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCT    200
gRNA                                                          GACTGGCCT

Wildtype         238 GGGGTCT-CATGTGGTAACCGTCATTGCCGAGTTACAGTTTTAAATCTTA    286
                     ||||||| ||||||||||||||||||||||||||||||||||||||||||
Mutation         201 GGGGTCTCCATGTGGTAACCGTCATTGCCGAGTTACAGTTTTAAATCTTA    250
gRNA                 GACTGGC-CTGGGGTCTCATG

Wildtype         287 CTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGC    336
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGC    300

Wildtype         337 TTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCC    386
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCC    350

Wildtype         387 ATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA    436
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 ATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA    400

Wildtype         437 ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTT    486
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTT    450

Wildtype         487 GTTTCTGTTGGTCTTTCGTATAATATGATGGTAGGCGAAATTCCAGCAGA    536
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 GTTTCTGTTGGTCTTTCGTATAATATGATGGTAGGCGAAATTCCAGCAGA    500

Wildtype         537 ATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTT    586
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTT    550

Wildtype         587 TGACAGGAGGAATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACC    636
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 TGACAGGAGGAATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACC    600

Wildtype         637 TTTTCTGCAAGCAAGAATA    655
                     |||||||||||||||||||
Mutation         601 TTTTCTGCAAGCAAGAATA    619