Plant Crispr Database

Plant Information

Name: CRT0032-4-3

Seed stock: 5304
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0032-4

Transformation: CRT0032
transformation CRT0032 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0032-4-3 (T1 )	CRT0032	CTG0032-2	CGR0032-2	gene : Solyc03g096190 (CORE)	+1 bp /+1 bp Is tDNA present: No Mutation sequence: AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATACTACCTTATGTTCTTTGCTCATCAAAAATT CAGGTGAAAGGGAATGAAACAGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTTTCAAGATCAT GGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCTGGGGTCTCCATGTGGTAACCGTCATTGCCGAGTTACAGTT TTAAATCTTACTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGCTTTCTTAATGTCCTTAAGCT TCAAAATAATAGTTTTTCAGGTGAAATCCCATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTTGTTTCTGTTGGTCTTTCGTATAATATGATG GTAGGCGAAATTCCAGCAGAATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTTTGACAGGAGG AATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACCTTTTCTGCAAGCAAGAATA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 38 AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATA 87 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATA 50 Wildtype 88 CTACCTTATGTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAAC 137 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 CTACCTTATGTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAAC 100 Wildtype 138 AGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTT 187 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 AGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTT 150 Wildtype 188 TCAAGATCATGGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCT 237 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 TCAAGATCATGGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCT 200 gRNA GACTGGCCT Wildtype 238 GGGGTCT-CATGTGGTAACCGTCATTGCCGAGTTACAGTTTTAAATCTTA 286 \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 GGGGTCTCCATGTGGTAACCGTCATTGCCGAGTTACAGTTTTAAATCTTA 250 gRNA GACTGGC-CTGGGGTCTCATG Wildtype 287 CTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGC 336 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 CTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGC 300 Wildtype 337 TTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCC 386 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 301 TTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCC 350 Wildtype 387 ATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA 436 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 351 ATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA 400 Wildtype 437 ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTT 486 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 401 ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTT 450 Wildtype 487 GTTTCTGTTGGTCTTTCGTATAATATGATGGTAGGCGAAATTCCAGCAGA 536 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 451 GTTTCTGTTGGTCTTTCGTATAATATGATGGTAGGCGAAATTCCAGCAGA 500 Wildtype 537 ATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTT 586 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 501 ATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTT 550 Wildtype 587 TGACAGGAGGAATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACC 636 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 551 TGACAGGAGGAATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACC 600 Wildtype 637 TTTTCTGCAAGCAAGAATA 655 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 601 TTTTCTGCAAGCAAGAATA 619

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0032-4-3 (T1 )

CRT0032

CTG0032-2

CGR0032-2

gene : Solyc03g096190 (CORE)

+1 bp /+1 bp

Is tDNA present: No

Mutation sequence:

AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATACTACCTTATGTTCTTTGCTCATCAAAAATT
CAGGTGAAAGGGAATGAAACAGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTTTCAAGATCAT
GGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCTGGGGTCTCCATGTGGTAACCGTCATTGCCGAGTTACAGTT
TTAAATCTTACTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGCTTTCTTAATGTCCTTAAGCT
TCAAAATAATAGTTTTTCAGGTGAAATCCCATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA
ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTTGTTTCTGTTGGTCTTTCGTATAATATGATG
GTAGGCGAAATTCCAGCAGAATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTTTGACAGGAGG
AATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACCTTTTCTGCAAGCAAGAATA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype          38 AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATA     87
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATA     50

Wildtype          88 CTACCTTATGTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAAC    137
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 CTACCTTATGTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAAC    100

Wildtype         138 AGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTT    187
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 AGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTT    150

Wildtype         188 TCAAGATCATGGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCT    237
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCAAGATCATGGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCT    200
gRNA                                                          GACTGGCCT

Wildtype         238 GGGGTCT-CATGTGGTAACCGTCATTGCCGAGTTACAGTTTTAAATCTTA    286
                     ||||||| ||||||||||||||||||||||||||||||||||||||||||
Mutation         201 GGGGTCTCCATGTGGTAACCGTCATTGCCGAGTTACAGTTTTAAATCTTA    250
gRNA                 GACTGGC-CTGGGGTCTCATG

Wildtype         287 CTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGC    336
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGC    300

Wildtype         337 TTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCC    386
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCC    350

Wildtype         387 ATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA    436
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 ATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA    400

Wildtype         437 ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTT    486
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTT    450

Wildtype         487 GTTTCTGTTGGTCTTTCGTATAATATGATGGTAGGCGAAATTCCAGCAGA    536
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 GTTTCTGTTGGTCTTTCGTATAATATGATGGTAGGCGAAATTCCAGCAGA    500

Wildtype         537 ATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTT    586
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 ATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTT    550

Wildtype         587 TGACAGGAGGAATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACC    636
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 TGACAGGAGGAATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACC    600

Wildtype         637 TTTTCTGCAAGCAAGAATA    655
                     |||||||||||||||||||
Mutation         601 TTTTCTGCAAGCAAGAATA    619