Is tDNA present: Yes

Mutation sequence:

CATGCTTGGTATTTTGTAACAAATTCATAGAATGCAAGTCATTTTCCGTAAGTGTGAACGGATGACTAGTTGACTGCTAC
TGAAAGCAGTGATTCACTGAAATATTTGCTATGAAATGTGTATAAAGGCTTCATTGTCAGCATAAGTTTTTTCAGTGAGT
GAAGTTTTCTTTCTTTTGAAATGATGATGTTAAAGACAGTTGTATATGCATTAGCCATATTTTCTATTACCTTTCTCATA
CCTTTNATCTGGGCAAAATCCAAGATTTGAAGTTGAAGTTGCTGCTTTGAAAGCTTTCAAGAGCTCAATCTCTGACGACC
CTTTCAGTGCACTTGTGGATTGGACTGATGTAAATCACCATTGCAACTGGTCTGGAATCATATGTGATCCTTCTTCAAAT
CATGTCATCAACATTTCACTTATTGAGACACAGCTCAAAGGAGAAATTTCTCCGTTTCTAGGAAACCTCTCCAAACTCCA
GGTTCTTGATCTAACTTTGAATTCATTTACTGGAAATATTCCACCCCAGCTGGGTCATTGCACGGACCTGGTTGAGCTCG
TTTTTTACCAAAACTCTCTTTTTGGTGAAATTCCTGCCG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         562 AGGCTTCAACAACAACAATTTCACAGGCAAATTACCATCTGAAATTGGTA    611
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 AGGCTTCAACAACAACAATTTCACAGGCAAATTACCATCTGAAATTGGTA     50

Wildtype         612 ATTTGGCTAATCTTCAGTTGTTTGTAGCTTATACAAACAATTTGGTTGGT    661
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATTTGGCTAATCTTCAGTTGTTTGTAGCTTATACAAACAATTTGGTTGGT    100

Wildtype         662 TTTATGCCTACC-TCCATTGGAATGCTGACAGCTTTGCATACCCTTGATC    710
                     |||||||||||| |||||||||||||||||||||||||||||||||||||
Mutation         101 TTTATGCCTACCTTCCATTGGAATGCTGACAGCTTTGCATACCCTTGATC    150

Wildtype         711 TGAGCGAAAACCAGTTATCTGGACCTATACCACCAGAAATTGGCAACTTG    760
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TGAGCGAAAACCAGTTATCTGGACCTATACCACCAGAAATTGGCAACTTG    200

Wildtype         761 TCAAGTTTAGGAATTCTTCAGTTACATCTCAACTCTCTTTCTGGGAAAAT    810
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TCAAGTTTAGGAATTCTTCAGTTACATCTCAACTCTCTTTCTGGGAAAAT    250

Wildtype         811 CCCATCTGAACTTGGCCTCTGTATCAATCTTTTTACCTTGAACATGTATA    860
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CCCATCTGAACTTGGCCTCTGTATCAATCTTTTTACCTTGAACATGTATA    300

Wildtype         861 CTAATCAATTTACTGGAAGCATACCTCCTGAGCTTGGAAATTTAGAAAAC    910
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 CTAATCAATTTACTGGAAGCATACCTCCTGAGCTTGGAAATTTAGAAAAC    350

Wildtype         911 TTGCAAATGCTCAGATTGTATAACAATAAGTTGAACTCCAGTATACCTGC    960
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TTGCAAATGCTCAGATTGTATAACAATAAGTTGAACTCCAGTATACCTGC    400

Wildtype         961 CTCAATATTCCACCTGAAGTCATTAACTCATTTAGGACTCTCACAGAATG   1010
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 CTCAATATTCCACCTGAAGTCATTAACTCATTTAGGACTCTCACAGAATG    450

Wildtype        1011 AGCTAACTGGGAATATTCCT   1030
                     ||||||||||||||||||||
Mutation         451 AGCTAACTGGGAATATTCCT    470