Is tDNA present: No

Mutation sequence:

TGAGAGACACGAGCCTGTGGATGTCACGTCATGGACGCGTTTACAAGGACGAAGTAGAAAAAGGAGAACGATTCATGATT
TTCAAAGAAAACATGAAATTCATTGAGTCGGTCAATAAGGCAGGGAATCTGTCTTACATAGGGATGAATGAATTTGCAGA
TATTACTTCACAAGAGTTTTTGGCTAAATTTACTGGATTAAACATACCTAATTCATATCTTTCACCTTCTCCAATGTCAT
CAACGGAATTTAAAAAAATTAATGATCTTAGCGATGATTACATGCCGTCTAACTTGGACTGGAGAGAGTCTGGGGCTGTC
ACTCAAGTGAAACATCAAGGTCGATGTGGTAAGATAGAACACTGTCTCATGAAATATTATTCACTGATATTAATTAATTA
GGTATTTTTTTTGTATTATTAGGATGTTGCTGGGCGTTTTCTGCAGTTGGATCACTAGAAGGAGCCTACAAAATTGCAAC
AGGCAACTTGATGGAATTCTCTGAACAGGAACTTCTCGATTGCACCACCAAC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         188 GAGAGACACGAGCTGTGGATGTCACGTCATGGACGCGTTTACAAGGACGA    237
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 GAGAGACACGAGCTGTGGATGTCACGTCATGGACGCGTTTACAAGGACGA     50

Wildtype         238 AGTAGAAAAAGGAGAACGATTCATGATTTTCAAAGAAAACATGAAATTCA    287
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AGTAGAAAAAGGAGAACGATTCATGATTTTCAAAGAAAACATGAAATTCA    100

Wildtype         288 TTGAGTCGGTCAATAAGGCAGGGAATCTGTCTTACAAGTTAGGGATGAAT    337
                     |||||||||||||||||||||||||||             ||||||||||
Mutation         101 TTGAGTCGGTCAATAAGGCAGGGAATC-------------AGGGATGAAT    137
gRNA                                      GGAATCTGTCTTACAAGTTA

Wildtype         338 GAATTTGCAGATATTACTTCACAAGAGTTTTTGGCTAAATTTACTGGATT    387
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         138 GAATTTGCAGATATTACTTCACAAGAGTTTTTGGCTAAATTTACTGGATT    187

Wildtype         388 AAACATACCTAATTCATATCTTTCACCTTCTCCAATGTCATCAACGGAAT    437
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         188 AAACATACCTAATTCATATCTTTCACCTTCTCCAATGTCATCAACGGAAT    237

Wildtype         438 TTAAAAAAATTAATGATCTTAGCGATGATTACATGCCGTCTAACTTGGAC    487
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         238 TTAAAAAAATTAATGATCTTAGCGATGATTACATGCCGTCTAACTTGGAC    287

Wildtype         488 TGGAGAGAGTCTGGGGCTGTCACTCAAGTGAAACATCAAGGTCGATGTGG    537
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         288 TGGAGAGAGTCTGGGGCTGTCACTCAAGTGAAACATCAAGGTCGATGTGG    337

Wildtype         538 TAAGATAGAACACTGTCTCATGAAATATTATTCACTGATATTAATTAATT    587
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         338 TAAGATAGAACACTGTCTCATGAAATATTATTCACTGATATTAATTAATT    387

Wildtype         588 AGGTATTTTTTTTGTATTATTAGGATGTTGCTGGGCGTTTTCTGCAGTTG    637
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         388 AGGTATTTTTTTTGTATTATTAGGATGTTGCTGGGCGTTTTCTGCAGTTG    437

Wildtype         638 GATCACTAGAAGGAGCCTACAAAATTGCAACAGGCAACTTGATGGAATTC    687
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         438 GATCACTAGAAGGAGCCTACAAAATTGCAACAGGCAACTTGATGGAATTC    487

Wildtype         688 TCTGAACAGGAACTTCTCGATTGCACCACCA    718
                     |||||||||||||||||||||||||||||||
Mutation         488 TCTGAACAGGAACTTCTCGATTGCACCACCA    518