Is tDNA present: Yes

Mutation sequence:

GTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTAGTAACTTCCCGCAACTTAAAAGAATTATCCATGCTTG
AAAGGCATGAAAATTGGATGGTTCATCATGGACGTGTATACAAAGATGATATAGAAAAAGAACACCGCTTCAAAACATTC
AAGGAAAACGTTGAGTTCATCGAATCTTTCAACAAGAACGGAACTCAACGTTATAAGCTAGCCATCAATAAATATGCTGA
TCTGACCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTATCCCAGCAAGAATCAACAGCTACAA
CAACGTCCTTTAAATATGATAGTGTGACTGAAGTTCCAAATAGCATGGACTGGAGAAAGAGAGGGAGTGTCACAGGAGTC
AAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCGTTTCACTTGAGTTGTGGTTAAGTATGTTCGTCTTA
AAATATTGGTTATGCTTGTCGAGAATCAAACTATATGAATTTTAATATATAACTTAATATCATGTATTTTTTCGTCATAT
TGATACGAGAAGATCAATTCCTACTTATTGTTATTTAATTTTCATTTAGATTTTAAGTATCTAAATTATAATTTTAAAAG
GTGACCACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 TATTCACTCAGCCTCTCTTTTAGTTTTTAACTTAGTTCCTATTTTGATAT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          63 TATTCACTCAGCCTCTCTTTTAGTTTTTAACTTAGTTCCTATTTTGATAT    112

Wildtype          51 TTCATTACCATTTACCATCAATGGCTTCCAATTTTTTCCTCAAGAATATT    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         113 TTCATTACCATTTACCATCAATGGCTTCCAATTTTTTCCTCAAGAATATT    162

Wildtype         101 ACTGTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTATAGT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         163 ACTGTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTATAGT    212
gRNA                                                            TTATAGT

Wildtype         151 AACTTCCCGCAACTTAAAAGAATTATCCATGCTTGAAAGGCATGAAAATT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         213 AACTTCCCGCAACTTAAAAGAATTATCCATGCTTGAAAGGCATGAAAATT    262
gRNA                 TTATAGTAACTTCCCGCAAC

Wildtype         201 GGATGGTTCATCATGGACGTGTATACAAAGATGATATAGAAAAAGAACAC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         263 GGATGGTTCATCATGGACGTGTATACAAAGATGATATAGAAAAAGAACAC    312

Wildtype         251 CGCTTCAAAACATTCAAGGAAAACGTTGAGTTCATCGAATCTTTCAACAA    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         313 CGCTTCAAAACATTCAAGGAAAACGTTGAGTTCATCGAATCTTTCAACAA    362

Wildtype         301 GAACGGAACTCAACGTTATAAGCTAGCCATCAATAAATATGCTGATCTGA    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         363 GAACGGAACTCAACGTTATAAGCTAGCCATCAATAAATATGCTGATCTGA    412

Wildtype         351 CCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTA    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         413 CCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTA    462

Wildtype         401 TCCCAGCAAGAATCAACAGCTACAACAACGTCCTTTAAATATGATAGTGT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         463 TCCCAGCAAGAATCAACAGCTACAACAACGTCCTTTAAATATGATAGTGT    512

Wildtype         451 GACTGAAGTTCCAAATAGCATGGACTGGAGAAAGAGAGGGAGTGTCACAG    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         513 GACTGAAGTTCCAAATAGCATGGACTGGAGAAAGAGAGGGAGTGTCACAG    562

Wildtype         501 GAGTCAAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         563 GAGTCAAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCG    612

Wildtype         551 TTTCACTTGAGTTGTGGTTAAGTATGTTCGTCTTAAAATATTGGTTATGC    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         613 TTTCACTTGAGTTGTGGTTAAGTATGTTCGTCTTAAAATATTGGTTATGC    662

Wildtype         601 TTGTCGAGAATCAAACTATATGAATTTTAATATATAACTTAATATCATGT    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         663 TTGTCGAGAATCAAACTATATGAATTTTAATATATAACTTAATATCATGT    712

Wildtype         651 ATTTTTTCGTCATATTGATACGAGAAGATCAATTCCTACTTATTGTTATT    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         713 ATTTTTTCGTCATATTGATACGAGAAGATCAATTCCTACTTATTGTTATT    762

Wildtype         701 TAATTTTCATTTAGATTTTAAGTATCTAAATTATAATTTTAAAAGGTGAC    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         763 TAATTTTCATTTAGATTTTAAGTATCTAAATTATAATTTTAAAAGGTGAC    812

Wildtype         751 CACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTTGAAAATTCTAT    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         813 CACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTTGAAAATTCTAT    862

Wildtype         801 ATGCCAATAACCATCATGTGTAACTATAAA    830
                     ||||||||||||||||||||||||||||||
Mutation         863 ATGCCAATAACCATCATGTGTAACTATAAA    892