Plant Crispr Database

Plant Information

Name: CRT0050-4

Seed stock: 5300
Seed availability: No

Relationship:
Progeny: CRT0050-4-3
Progeny: CRT0050-4-2
Progeny: CRT0050-4-1

Transformation: CRT0050
transformation CRT0050 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0050-4 (T0 )	CRT0050	CTG0050-2	CGR0050-2	gene : Solyc01g067900 (RALF1)	-47 bp/-47 bp Is tDNA present: Yes Mutation sequence: AAAATCACATGCCTCTCCCCTTATTATTCCACATCTCAAAAAAGAAGAGGAGAAAACACCCTTTTAAATCCTTATATATT TACTCCAATCCCACTTTTCTCTCTCTTTATCAAAAATCTCCTTTAATTTTCTCCTCCTTTCCCTAACCCCTACCCCAAAA AATGGCGAAAGTGAAATCTCTCTCTATTTTCTTCATCTCATCCATTTTCTCCGTCGTTATTGTAGCGCTTTTATCTCCGG CCGCCGCTGGTTCCGCGGCGGCAGTAGCTGCTACTGGGTCCCACCAGCTGAGTTACTTTCCGATGACATTATCTTCTTCT TCTTCACCGATCTGCGACGGTTCGATCGGGGACTGTTTGGCTGAAGAAGACGAGAATGAGTTCGGGATGGAATCGGAGAG CAGCCGGCGCATGTTAGCATACCGCCGGAGATACATTAGCTACGTACTACAATTGCCGTCCCGGAGCTCAGGCGAATCCT TATCAACGTGGATGCAGTGCCATCACGCGCTGCCGTCATTGAAAACCCTCTACACAGTAAATTCCTTGATTTACTGTATT TACATTTTTTTTTTACCAGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 291 ACTTTCCGATGACATTATCTTCTTCTTCTTCACCGATCTGCGACGGTTCG 340 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ACTTTCCGATGACATTATCTTCTTNTTCTTCACCGATNTGCGACGGTTCG 50 Wildtype 341 ATCGGGGACTGTTTGGCTGAAGAAGACGAGAATGAGTTCGGGATGGAATC 390 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 ATCGGGGACTGTTTGGCTGAAGAAGACGAGAATGAGTTCGGGATGGAATC 100 Wildtype 391 GGAGAGCAGCCGGCGCATGTTAGCATACCGCCGGAGATACATTAGCTACG 440 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\| MutationRev 101 GGAGAGCAGCCGGCGCATGTTAGCATACCGCCGGAGATACNTTAGCTACG 150 Wildtype 441 GCGCGCTTAGTAGTAACAGAGTGCCGTGTTCAAGGAGAGGAGCTTCGTAC 490 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GCGCGCTTAGTAGTAACAGAGTGCCGTGT----GGAGAGGAGCTTCGTAC 196 gRNA TGTTCAAGGAGAGGAGCTTC Wildtype 491 TACAATTGCCGTCCCGGAGCTCAGGCGAATCCTTATCAACGTGGATGCAG 540 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 197 TACAATTGCCGTCCCGGAGCTCAGGCGAATCCTTATCAACGTGGATGCAG 246 Wildtype 541 TGCCATCACGCGCTGCCGTCATTGAAAACCCTCTACACAGTAAATTCCTT 590 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 247 TGCCATCACGCGCTGCCGTCATTGAAAACCCTCTACACAGTAAATTCCTT 296 Wildtype 591 GATTT-ACTGTATTTACATTTTTTTTTTACCAGAGTGGTAAAAAAGTTAA 639 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 297 GATTTAACTGTATTTACATTTTTTTTTTACCAGAGTGGTAAAAAAGTTAA 346 Wildtype 640 AAAATTCTTTTTGAGCTTTGTGTGATCTATTTA 672 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 347 AAAATTCTTTTTGAGCTTTGTGTGATCTATTTA 379

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0050-4 (T0 )

CRT0050

CTG0050-2

CGR0050-2

gene : Solyc01g067900 (RALF1)

-47 bp/-47 bp

Is tDNA present: Yes

Mutation sequence:

AAAATCACATGCCTCTCCCCTTATTATTCCACATCTCAAAAAAGAAGAGGAGAAAACACCCTTTTAAATCCTTATATATT
TACTCCAATCCCACTTTTCTCTCTCTTTATCAAAAATCTCCTTTAATTTTCTCCTCCTTTCCCTAACCCCTACCCCAAAA
AATGGCGAAAGTGAAATCTCTCTCTATTTTCTTCATCTCATCCATTTTCTCCGTCGTTATTGTAGCGCTTTTATCTCCGG
CCGCCGCTGGTTCCGCGGCGGCAGTAGCTGCTACTGGGTCCCACCAGCTGAGTTACTTTCCGATGACATTATCTTCTTCT
TCTTCACCGATCTGCGACGGTTCGATCGGGGACTGTTTGGCTGAAGAAGACGAGAATGAGTTCGGGATGGAATCGGAGAG
CAGCCGGCGCATGTTAGCATACCGCCGGAGATACATTAGCTACGTACTACAATTGCCGTCCCGGAGCTCAGGCGAATCCT
TATCAACGTGGATGCAGTGCCATCACGCGCTGCCGTCATTGAAAACCCTCTACACAGTAAATTCCTTGATTTACTGTATT
TACATTTTTTTTTTACCAGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         291 ACTTTCCGATGACATTATCTTCTTCTTCTTCACCGATCTGCGACGGTTCG    340
                     ||||||||||||||||||||||||.||||||||||||.||||||||||||
MutationRev        1 ACTTTCCGATGACATTATCTTCTTNTTCTTCACCGATNTGCGACGGTTCG     50

Wildtype         341 ATCGGGGACTGTTTGGCTGAAGAAGACGAGAATGAGTTCGGGATGGAATC    390
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 ATCGGGGACTGTTTGGCTGAAGAAGACGAGAATGAGTTCGGGATGGAATC    100

Wildtype         391 GGAGAGCAGCCGGCGCATGTTAGCATACCGCCGGAGATACATTAGCTACG    440
                     ||||||||||||||||||||||||||||||||||||||||.|||||||||
MutationRev      101 GGAGAGCAGCCGGCGCATGTTAGCATACCGCCGGAGATACNTTAGCTACG    150

Wildtype         441 GCGCGCTTAGTAGTAACAGAGTGCCGTGTTCAAGGAGAGGAGCTTCGTAC    490
                     |||||||||||||||||||||||||||||    |||||||||||||||||
MutationRev      151 GCGCGCTTAGTAGTAACAGAGTGCCGTGT----GGAGAGGAGCTTCGTAC    196
gRNA                                           TGTTCAAGGAGAGGAGCTTC

Wildtype         491 TACAATTGCCGTCCCGGAGCTCAGGCGAATCCTTATCAACGTGGATGCAG    540
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      197 TACAATTGCCGTCCCGGAGCTCAGGCGAATCCTTATCAACGTGGATGCAG    246

Wildtype         541 TGCCATCACGCGCTGCCGTCATTGAAAACCCTCTACACAGTAAATTCCTT    590
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      247 TGCCATCACGCGCTGCCGTCATTGAAAACCCTCTACACAGTAAATTCCTT    296

Wildtype         591 GATTT-ACTGTATTTACATTTTTTTTTTACCAGAGTGGTAAAAAAGTTAA    639
                     ||||| ||||||||||||||||||||||||||||||||||||||||||||
MutationRev      297 GATTTAACTGTATTTACATTTTTTTTTTACCAGAGTGGTAAAAAAGTTAA    346

Wildtype         640 AAAATTCTTTTTGAGCTTTGTGTGATCTATTTA    672
                     |||||||||||||||||||||||||||||||||
MutationRev      347 AAAATTCTTTTTGAGCTTTGTGTGATCTATTTA    379