Is tDNA present: Yes

Mutation sequence:

ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype          63 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC    112
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC     50

Wildtype         113 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC    162
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC    100

Wildtype         163 GCCATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTG    212
                     |||||||||||||||||||||||||| |||||||||||||||||||||||
Mutation         101 GCCATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTG    149
gRNA                           GGCGCTTAAAGCGGCCTTGG

Wildtype         213 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC    262
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         150 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC    199

Wildtype         263 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG    312
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         200 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG    249

Wildtype         313 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC    362
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC    299

Wildtype         363 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC    412
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC    349

Wildtype         413 TTTGTTCCGTGG    424
                     ||||||||||||
Mutation         350 TTTGTTCCGTGG    361