Plant Crispr Database

Plant Information

Name: CRT0059-8-5

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0059-8
Progeny: CRT0059-8-5-2

Transformation: CRT0059
transformation CRT0059 to RG-prf3

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation
CRT0059-8-5 (T1 )	CRT0059	CTG0059-1	CGR0059-1	gene : Solyc11g069430 (Aquaporin transporter 1)	WT 26.4%; +1bp (70.8%)
CRT0059-8-5 (T1 )	CRT0059	CTG0059-1	CGR0059-2	gene : Solyc06g074820 (Aquaporin transporter 2)	-1 bp/-1 bp Is tDNA present: Yes Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG GCATGGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 63 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC 112 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC 50 Wildtype 113 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC 162 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC 100 Wildtype 163 GCCATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTG 212 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 GCCATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTG 149 gRNA GGCGCTTAAAGCGGCCTTGG Wildtype 213 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC 262 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 150 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC 199 Wildtype 263 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG 312 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 200 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG 249 Wildtype 313 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC 362 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 250 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC 299 Wildtype 363 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC 412 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 300 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC 349 Wildtype 413 TTTGTTCCGTGG 424 \|\|\|\|\|\|\|\|\|\|\|\| Mutation 350 TTTGTTCCGTGG 361
CRT0059-8-5 (T1 )	CRT0059	CTG0059-1	CGR0059-3	gene : Solyc08g066840 (Aquaporin transporter 3)	-124 bp/-124 bp Is tDNA present: Yes Mutation sequence: TACAAGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAA TGAAAAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAG ATTCCACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCTCCATAATTAAC CCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAAAAAATGTACATATTAACAAAGAATT TCAAAACTTTTATCATTAACTGTGTCATTAGATCTTAAATTTCATCGTTGATTAAAACGG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 230 CCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGAA 279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 CCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGAA 50 Wildtype 280 AAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAA 329 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 AAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAA 100 Wildtype 330 TCCAGTAGACCCAATGGTCAGTCCAGATTCCACTTACAAAAGCTGGCCCA 379 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TCCAGTAGACCCAATGGTCAGTCCAGATTCCACTTACAAAAGCTGGCCCA 150 Wildtype 380 AATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCAGCCAT 429 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 AATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACC------ 194 gRNA ACCAGCCAT Wildtype 430 AATATTGGCCCCAACAACAAGCCCAGTTAGAAGTGGGCCCATTCCTTCAA 479 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 195 AATATTGGCCCCAACAACAAGCCCAGTTAGAAGTGGGCCCATTCCTTCAA 244 gRNA ACCAGCCATAATATTGGCCC Wildtype 480 GAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAACAAA 529 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 245 GAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAACAAA 294 Wildtype 530 GAAAAGGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCC 579 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 295 GAAAAGGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCC 344 Wildtype 580 ATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAAAAAATGTACATATTA 629 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 345 ATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAAAAAATGTACATATTA 394 Wildtype 630 ACAAAGAAT 638 \|\|\|\|\|\|\|\|\| MutationRev 395 ACAAAGAAT 403

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0059-8-5 (T1 )

CRT0059

CTG0059-1

CGR0059-1

gene : Solyc11g069430 (Aquaporin transporter 1)

WT 26.4%; +1bp (70.8%)

CRT0059-8-5 (T1 )

CRT0059

CTG0059-1

CGR0059-2

gene : Solyc06g074820 (Aquaporin transporter 2)

-1 bp/-1 bp

Is tDNA present: Yes

Mutation sequence:

ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype          63 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC    112
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC     50

Wildtype         113 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC    162
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC    100

Wildtype         163 GCCATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTG    212
                     |||||||||||||||||||||||||| |||||||||||||||||||||||
Mutation         101 GCCATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTG    149
gRNA                           GGCGCTTAAAGCGGCCTTGG

Wildtype         213 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC    262
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         150 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC    199

Wildtype         263 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG    312
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         200 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG    249

Wildtype         313 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC    362
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC    299

Wildtype         363 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC    412
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC    349

Wildtype         413 TTTGTTCCGTGG    424
                     ||||||||||||
Mutation         350 TTTGTTCCGTGG    361

CRT0059-8-5 (T1 )

CRT0059

CTG0059-1

CGR0059-3

gene : Solyc08g066840 (Aquaporin transporter 3)

-124 bp/-124 bp

Is tDNA present: Yes

Mutation sequence:

TACAAGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAA
TGAAAAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAG
ATTCCACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCTCCATAATTAAC
CCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAAAAAATGTACATATTAACAAAGAATT
TCAAAACTTTTATCATTAACTGTGTCATTAGATCTTAAATTTCATCGTTGATTAAAACGG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         230 CCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGAA    279
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 CCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGAA     50

Wildtype         280 AAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAA    329
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 AAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAA    100

Wildtype         330 TCCAGTAGACCCAATGGTCAGTCCAGATTCCACTTACAAAAGCTGGCCCA    379
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 TCCAGTAGACCCAATGGTCAGTCCAGATTCCACTTACAAAAGCTGGCCCA    150

Wildtype         380 AATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCAGCCAT    429
                     ||||||||||||||||||||||||||||||||||||||||||||      
MutationRev      151 AATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACC------    194
gRNA                                                          ACCAGCCAT

Wildtype         430 AATATTGGCCCCAACAACAAGCCCAGTTAGAAGTGGGCCCATTCCTTCAA    479
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      195 AATATTGGCCCCAACAACAAGCCCAGTTAGAAGTGGGCCCATTCCTTCAA    244
gRNA                 ACCAGCCATAATATTGGCCC

Wildtype         480 GAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAACAAA    529
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      245 GAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAACAAA    294

Wildtype         530 GAAAAGGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCC    579
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      295 GAAAAGGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCC    344

Wildtype         580 ATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAAAAAATGTACATATTA    629
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      345 ATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAAAAAATGTACATATTA    394

Wildtype         630 ACAAAGAAT    638
                     |||||||||
MutationRev      395 ACAAAGAAT    403