Plant Crispr Database

Plant Information

Name: CRT0060-2-23

Seed stock: 5334
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0060-2

Transformation: CRT0060
transformation CRT0060 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0060-2-23 (T1 )	CRT0060	CTG0060-1	CGR0060-1	gene : Solyc05g005700 (ADE )	-5 bp/-5 bp Is tDNA present: No Mutation sequence: TTTGAGGTAGAAATCTTATTGAAATGGTTGAATATTCTGAGATGAAATGTAGAGAACTATAATGAGAAAAACTATTTTGT GTAGGAAAAAGATCCCATCTTGGCAAAATAGCTGCTTATAAATACAGCACAGCGGCTGCTTTCGAGGAGCCCGTCAAGCC TACATGTGGATCATACTAAACTTCTTATCAACGGGCAATTTGTTGATTCTGCATCAGGTAAATTTGTTAATCCATGTTAA TCACCATAATGGATCTCAATTCTCTTCTTTATACTTCTATTTTGGCCCTTTTGTTGTACAATGGAGCTAATTCGTTGAAC TTTTAGGAAAAACTTTCCCGACTCTCGACCCCAGGACAGGGGAGGTAATTGCACATATTGCTGAAGGTGATGCAGAAGAT ATTAATCGGGCAGTTGCTGCTGCTCGTAAGGCTTTCGATGAAGGACCATGGCCTAGGATGACTGCTTATGTATCTACTTT GTATCTCTTAGTGCATTCTTTTGCTATGTTAATGAATGTGCATGCGAGATTTCATCTACTCGTTAATGGTTCATAACTGC AGGAAAGGTCAAAGATATTGCTGCGCCTTGCTGATCTGATTGAAAAACATAACGAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2953 ATGTTTTTCAATCAGATCAGCAAGGCGCAGCAATATCTTTGACCTTTCCT 3002 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ATGTTTTTCAATCAGATCAGCAAGGCGCAGCAATATCTTTGACCTTTCCT 50 Wildtype 3003 GCAGTTATGAACCATTAACGAGTAGATGAAATCTCGCATGCACATTCATT 3052 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GCAGTTATGAACCATTAACGAGTAGATGAAATCTCGCATGCACATTCATT 100 Wildtype 3053 AACATAGCAAAAGAATGCACTAAGAGATACAAAGTAGATACATAAGCAGT 3102 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AACATAGCAAAAGAATGCACTAAGAGATACAAAGTAGATACATAAGCAGT 150 Wildtype 3103 CATCCTAGGCCATGGTCCTTCATCGAAAGCCTTACGAGCAGCAGCAACTG 3152 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 CATCCTAGGCCATGGTCCTTCATCGAA---------AGCAGCAGCAACTG 191 Wildtype 3153 CCCGATTAATATCTTCTGCATCACCTTCAGCAATATGTGCAATTACCTCC 3202 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 192 CCCGATTAATATCTTCTGCATCACCTTCAGCAATATGTGCAATTACCTCC 241 Wildtype 3203 CCTGTCCTGGGGTCGAGAGTCGGGAAAGTTTTTCCTAAAAGTTCAACGAA 3252 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 242 CCTGTCCTGGGGTCGAGAGTCGGGAAAGTTTTTCCTAAAAGTTCAACGAA 291 Wildtype 3253 TTAGCTCCATTGTACAACAAAAGGGCCAAAATAGAAGTATAAAGAAGAGA 3302 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 292 TTAGCTCCATTGTACAACAAAAGGGCCAAAATAGAAGTATAAAGAAGAGA 341 Wildtype 3303 ATTGAGATCCATTATGGTGATTAACATGGATTAACAAATTTACCTGATGC 3352 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 342 ATTGAGATCCATTATGGTGATTAACATGGATTAACAAATTTACCTGATGC 391 Wildtype 3353 AGAATCAACAAATTGCCCGTTGATAAGAAGTTTAGTATGATCCACATTGA 3402 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 392 AGAATCAACAAATTGCCCGTTGATAAGAAGTTTAGTATGATCCACAT--- 438 gRNA GTATGATCCACATTGA Wildtype 3403 CAGTAGGCTTGACGGGCTCCTCGAAAGCAGCCGCTGTGCTGTATTTATAA 3452 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 439 -AGTAGGCTTGACGGGCTCCTCGAAAGCAGCCGCTGTGCTGTATTTATAA 487 gRNA GTATGATCCACATTGACAGT Wildtype 3453 GCAGCTATTTTGCCAAGATGGGATCTTTTTCCTACACAAAATAGTTTTTC 3502 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 488 GCAGCTATTTTGCCAAGATGGGATCTTTTTCCTACACAAAATAGTTTTTC 537 Wildtype 3503 TCATTATAGTTCTCTACATTTCATCTCAGAATATTCAACCATTTCAATAA 3552 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 538 TCATTATAGTTCTCTACATTTCATCTCAGAATATTCAACCATTTCAATAA 587 Wildtype 3553 GATTTCTACCTCAAAGAT 3570 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 588 GATTTCTACCTCAAAGAT 605

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0060-2-23 (T1 )

CRT0060

CTG0060-1

CGR0060-1

gene : Solyc05g005700 (ADE )

-5 bp/-5 bp

Is tDNA present: No

Mutation sequence:

TTTGAGGTAGAAATCTTATTGAAATGGTTGAATATTCTGAGATGAAATGTAGAGAACTATAATGAGAAAAACTATTTTGT
GTAGGAAAAAGATCCCATCTTGGCAAAATAGCTGCTTATAAATACAGCACAGCGGCTGCTTTCGAGGAGCCCGTCAAGCC
TACATGTGGATCATACTAAACTTCTTATCAACGGGCAATTTGTTGATTCTGCATCAGGTAAATTTGTTAATCCATGTTAA
TCACCATAATGGATCTCAATTCTCTTCTTTATACTTCTATTTTGGCCCTTTTGTTGTACAATGGAGCTAATTCGTTGAAC
TTTTAGGAAAAACTTTCCCGACTCTCGACCCCAGGACAGGGGAGGTAATTGCACATATTGCTGAAGGTGATGCAGAAGAT
ATTAATCGGGCAGTTGCTGCTGCTCGTAAGGCTTTCGATGAAGGACCATGGCCTAGGATGACTGCTTATGTATCTACTTT
GTATCTCTTAGTGCATTCTTTTGCTATGTTAATGAATGTGCATGCGAGATTTCATCTACTCGTTAATGGTTCATAACTGC
AGGAAAGGTCAAAGATATTGCTGCGCCTTGCTGATCTGATTGAAAAACATAACGAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2953 ATGTTTTTCAATCAGATCAGCAAGGCGCAGCAATATCTTTGACCTTTCCT   3002
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 ATGTTTTTCAATCAGATCAGCAAGGCGCAGCAATATCTTTGACCTTTCCT     50

Wildtype        3003 GCAGTTATGAACCATTAACGAGTAGATGAAATCTCGCATGCACATTCATT   3052
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GCAGTTATGAACCATTAACGAGTAGATGAAATCTCGCATGCACATTCATT    100

Wildtype        3053 AACATAGCAAAAGAATGCACTAAGAGATACAAAGTAGATACATAAGCAGT   3102
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AACATAGCAAAAGAATGCACTAAGAGATACAAAGTAGATACATAAGCAGT    150

Wildtype        3103 CATCCTAGGCCATGGTCCTTCATCGAAAGCCTTACGAGCAGCAGCAACTG   3152
                     |||||||||||||||||||||||||||         ||||||||||||||
MutationRev      151 CATCCTAGGCCATGGTCCTTCATCGAA---------AGCAGCAGCAACTG    191

Wildtype        3153 CCCGATTAATATCTTCTGCATCACCTTCAGCAATATGTGCAATTACCTCC   3202
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      192 CCCGATTAATATCTTCTGCATCACCTTCAGCAATATGTGCAATTACCTCC    241

Wildtype        3203 CCTGTCCTGGGGTCGAGAGTCGGGAAAGTTTTTCCTAAAAGTTCAACGAA   3252
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      242 CCTGTCCTGGGGTCGAGAGTCGGGAAAGTTTTTCCTAAAAGTTCAACGAA    291

Wildtype        3253 TTAGCTCCATTGTACAACAAAAGGGCCAAAATAGAAGTATAAAGAAGAGA   3302
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      292 TTAGCTCCATTGTACAACAAAAGGGCCAAAATAGAAGTATAAAGAAGAGA    341

Wildtype        3303 ATTGAGATCCATTATGGTGATTAACATGGATTAACAAATTTACCTGATGC   3352
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      342 ATTGAGATCCATTATGGTGATTAACATGGATTAACAAATTTACCTGATGC    391

Wildtype        3353 AGAATCAACAAATTGCCCGTTGATAAGAAGTTTAGTATGATCCACATTGA   3402
                     |||||||||||||||||||||||||||||||||||||||||||||||   
MutationRev      392 AGAATCAACAAATTGCCCGTTGATAAGAAGTTTAGTATGATCCACAT---    438
gRNA                                                   GTATGATCCACATTGA

Wildtype        3403 CAGTAGGCTTGACGGGCTCCTCGAAAGCAGCCGCTGTGCTGTATTTATAA   3452
                      |||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      439 -AGTAGGCTTGACGGGCTCCTCGAAAGCAGCCGCTGTGCTGTATTTATAA    487
gRNA                 GTATGATCCACATTGACAGT

Wildtype        3453 GCAGCTATTTTGCCAAGATGGGATCTTTTTCCTACACAAAATAGTTTTTC   3502
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      488 GCAGCTATTTTGCCAAGATGGGATCTTTTTCCTACACAAAATAGTTTTTC    537

Wildtype        3503 TCATTATAGTTCTCTACATTTCATCTCAGAATATTCAACCATTTCAATAA   3552
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      538 TCATTATAGTTCTCTACATTTCATCTCAGAATATTCAACCATTTCAATAA    587

Wildtype        3553 GATTTCTACCTCAAAGAT   3570
                     ||||||||||||||||||
MutationRev      588 GATTTCTACCTCAAAGAT    605