TATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTAACCTGCACCATGAGCTTACTTGACAAGCTC
TGGGACGACACCGTTGCCGGTCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTTTTAGTCAGCGTTC
AAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTCGCCGGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACT
ATCTATTATGATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGTATATTTATCTGTGAACGTGCACA
AATTAGTGCGATTTAAATAACGCTGTCTATTATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTTAAC
TGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGCAAGTTACATTTTCCTAAATAAGGACTAGATGAT
AGCTTATCAAAAAGAGAAGGAAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGCTACCGAAAATATT
CGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAGAATCAGAAGTTTCAACACCGAGATACTGAGGAAGCAAGTGAGG
ACGCGGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCAGAATAGAGATTCACCTCCCGTTTCTCCG
GCCGGTACTACTCCTCCGGTATCTCCTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAACTAGTTCC
TACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAATAATTTGT

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype          54 TATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTA    103
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTA     50

Wildtype         104 ACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGCCGG    153
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGCCGG    100

Wildtype         154 TCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTTTTA    203
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTTTTA    150

Wildtype         204 GTCAGCGTTCAAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTCGCC    253
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 GTCAGCGTTCAAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTCGCC    200

Wildtype         254 GGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATTATG    303
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 GGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATTATG    250

Wildtype         304 ATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGTATA    353
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 ATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGTATA    300

Wildtype         354 TTTATCTGTGAACGTGCACAAATTAGTGCGATTTAAATAACGCTGTCTAT    403
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TTTATCTGTGAACGTGCACAAATTAGTGCGATTTAAATAACGCTGTCTAT    350

Wildtype         404 TATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTTAAC    453
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTTAAC    400

Wildtype         454 TGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGCAAG    503
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 TGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGCAAG    450

Wildtype         504 TTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCAAAAAGAGAAGG    553
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCAAAAAGAGAAGG    500

Wildtype         554 AAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGCTAC    603
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 AAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGCTAC    550

Wildtype         604 CGAAAATATTCGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAGAAT    653
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 CGAAAATATTCGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAGAAT    600

Wildtype         654 CAGAAGTTTCAACACCGAGATCCTTTACTGAGGAAGCAAGTGAGGACGCG    703
                     ||||||||||||||||||||     |||||||||||||||||||||||||
Mutation         601 CAGAAGTTTCAACACCGAGA-----TACTGAGGAAGCAAGTGAGGACGCG    645
gRNA                                  AGATCCTTTACTGAGGAAGC

Wildtype         704 GTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCAGAA    753
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         646 GTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCAGAA    695

Wildtype         754 TAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTATCTC    803
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         696 TAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTATCTC    745

Wildtype         804 CTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAACTA    853
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         746 CTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAACTA    795

Wildtype         854 GTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAATAA    903
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         796 GTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAATAA    845

Wildtype         904 TTTGT    908
                     |||||
Mutation         846 TTTGT    850