Is tDNA present: Yes

Mutation sequence:

CCCTTCTCTTATATATACAGAACCATATTGTCTATCTGGGCATCTCATTCTACCCTGAGTAACTGGATAGGTAACAATCA
TCAACTTCCTCAGTTGTGACACATATCGAAAAATTTTACCAAAAACTTAAACATGTACAAAATGATGTGTGTAATCGTCT
TTGGTGTTTTAGCCGTTGCAAATATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCATGAGTTGG
ATCGTACTAGCGCGGCAGCTTATACAAATTGGGCTGCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATT
TAGTTAATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACACATCCAATGTCTGATTATGTTACTTT
CCGTATTTTATACAGTATTCAATTTCATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATGGTTGC
AATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCCAATATGACTCTAAACTCTACCGGAGAACACTACTA
TATTTGCACTTTTGGTATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCACTGCAACTCCAGGAG
CCAACCCTCCTGTTGTACGACAACCAGAAGCTTGTGCACCTACACCCTCTGCTGCAGGACCTTCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGTACAAAATGATGTGTGTAATCGTCTTTGGTGTTTTAGCCGTTGCAAA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         133 ATGTACAAAATGATGTGTGTAATCGTCTTTGGTGTTTTAGCCGTTGCAAA    182

Wildtype          51 TATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCA    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         183 TATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCA    232

Wildtype         101 TGAGTTGGATCGTACCAAGCAATAGCGCGGCAGCTTATACAAATTGGGCT    150
                     |||||||||||||||       ||||||||||||||||||||||||||||
Mutation         233 TGAGTTGGATCGTAC-------TAGCGCGGCAGCTTATACAAATTGGGCT    275
gRNA                        GATCGTACCAAGCAATAGCG

Wildtype         151 GCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATTTAGTT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         276 GCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATTTAGTT    325

Wildtype         201 AATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACAC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         326 AATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACAC    375

Wildtype         251 ATCCAAAGTCTGATTATGTTACTTTCCGTATTTTATACAGTATTCAATTT    300
                     ||||||.|||||||||||||||||||||||||||||||||||||||||||
Mutation         376 ATCCAATGTCTGATTATGTTACTTTCCGTATTTTATACAGTATTCAATTT    425

Wildtype         301 CATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATG    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         426 CATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATG    475

Wildtype         351 GTTGCAATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCC    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         476 GTTGCAATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCC    525

Wildtype         401 AATATGACTCTAAACTCTACCGGAGAACACTACTATATTTGCACTTTTGG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         526 AATATGACTCTAAACTCTACCGGAGAACACTACTATATTTGCACTTTTGG    575

Wildtype         451 TATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCA    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         576 TATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCA    625

Wildtype         501 CTGCAACTCCAGGAGCCAACCCTCCTGTTGTACGACAACCAGAAGCTTGT    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         626 CTGCAACTCCAGGAGCCAACCCTCCTGTTGTACGACAACCAGAAGCTTGT    675

Wildtype         551 GCACCTACACCCTCTGCTGCAGGACCTTCA    580
                     ||||||||||||||||||||||||||||||
Mutation         676 GCACCTACACCCTCTGCTGCAGGACCTTCA    705