Plant Crispr Database

Plant Information

Name: CRT0062-1-1

Seed stock: 5604
Seed availability: Yes, please contact: gbm7@cornell.edu to request seeds

Relationship:
Parent: CRT0062-1

Transformation: CRT0062
transformation CRT0062 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0062-1-1 (T1 )	CRT0062	CTG0062-1	CGR0062-1	gene : Solyc03g116690 (PBCP )	-7 bp/-7 bp Is tDNA present: No Mutation sequence: CCCTTCTCTTATATATACAGAACCATATTGTCTATCTGGGCATCTCATTCTACCCTGAGTAACTGGATAGGTAACAATCA TCAACTTCCTCAGTTGTGACACATATCGAAAAATTTTACCAAAAACTTAAACATGTACAAAATGATGTGTGTAATCGTCT TTGGTGTTTTAGCCGTTGCAAATATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCATGAGTTGG ATCGTACTAGCGCGGCAGCTTATACAAATTGGGCTGCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATT TAGTTAATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACACATCCAATGTCTGATTATGTTACTTT CCGTATTTTATACAGTATTCAATTTCATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATGGTTGC AATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCCAATATGACTCTAAACTCTACCGGAGAACACTACTA TATTTGCACTTTTGGTATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCACTGCAACTCCAGGAG CCAACCCTCCTGTTGTACGACAACCAGAAGCTTGTGCACCTACACCCTCTGCTGCAGGACCTTCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1 ATGTACAAAATGATGTGTGTAATCGTCTTTGGTGTTTTAGCCGTTGCAAA 50 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 133 ATGTACAAAATGATGTGTGTAATCGTCTTTGGTGTTTTAGCCGTTGCAAA 182 Wildtype 51 TATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCA 100 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 183 TATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCA 232 Wildtype 101 TGAGTTGGATCGTACCAAGCAATAGCGCGGCAGCTTATACAAATTGGGCT 150 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 233 TGAGTTGGATCGTAC-------TAGCGCGGCAGCTTATACAAATTGGGCT 275 gRNA GATCGTACCAAGCAATAGCG Wildtype 151 GCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATTTAGTT 200 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 276 GCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATTTAGTT 325 Wildtype 201 AATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACAC 250 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 326 AATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACAC 375 Wildtype 251 ATCCAAAGTCTGATTATGTTACTTTCCGTATTTTATACAGTATTCAATTT 300 \|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 376 ATCCAATGTCTGATTATGTTACTTTCCGTATTTTATACAGTATTCAATTT 425 Wildtype 301 CATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATG 350 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 426 CATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATG 475 Wildtype 351 GTTGCAATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCC 400 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 476 GTTGCAATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCC 525 Wildtype 401 AATATGACTCTAAACTCTACCGGAGAACACTACTATATTTGCACTTTTGG 450 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 526 AATATGACTCTAAACTCTACCGGAGAACACTACTATATTTGCACTTTTGG 575 Wildtype 451 TATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCA 500 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 576 TATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCA 625 Wildtype 501 CTGCAACTCCAGGAGCCAACCCTCCTGTTGTACGACAACCAGAAGCTTGT 550 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 626 CTGCAACTCCAGGAGCCAACCCTCCTGTTGTACGACAACCAGAAGCTTGT 675 Wildtype 551 GCACCTACACCCTCTGCTGCAGGACCTTCA 580 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 676 GCACCTACACCCTCTGCTGCAGGACCTTCA 705

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0062-1-1 (T1 )

CRT0062

CTG0062-1

CGR0062-1

gene : Solyc03g116690 (PBCP )

-7 bp/-7 bp

Is tDNA present: No

Mutation sequence:

CCCTTCTCTTATATATACAGAACCATATTGTCTATCTGGGCATCTCATTCTACCCTGAGTAACTGGATAGGTAACAATCA
TCAACTTCCTCAGTTGTGACACATATCGAAAAATTTTACCAAAAACTTAAACATGTACAAAATGATGTGTGTAATCGTCT
TTGGTGTTTTAGCCGTTGCAAATATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCATGAGTTGG
ATCGTACTAGCGCGGCAGCTTATACAAATTGGGCTGCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATT
TAGTTAATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACACATCCAATGTCTGATTATGTTACTTT
CCGTATTTTATACAGTATTCAATTTCATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATGGTTGC
AATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCCAATATGACTCTAAACTCTACCGGAGAACACTACTA
TATTTGCACTTTTGGTATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCACTGCAACTCCAGGAG
CCAACCCTCCTGTTGTACGACAACCAGAAGCTTGTGCACCTACACCCTCTGCTGCAGGACCTTCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGTACAAAATGATGTGTGTAATCGTCTTTGGTGTTTTAGCCGTTGCAAA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         133 ATGTACAAAATGATGTGTGTAATCGTCTTTGGTGTTTTAGCCGTTGCAAA    182

Wildtype          51 TATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCA    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         183 TATGATGCAAGATGCAACAGCTCAAACGGTGCATGTGGTTGGGGACACCA    232

Wildtype         101 TGAGTTGGATCGTACCAAGCAATAGCGCGGCAGCTTATACAAATTGGGCT    150
                     |||||||||||||||       ||||||||||||||||||||||||||||
Mutation         233 TGAGTTGGATCGTAC-------TAGCGCGGCAGCTTATACAAATTGGGCT    275
gRNA                        GATCGTACCAAGCAATAGCG

Wildtype         151 GCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATTTAGTT    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         276 GCTGGGAAAACCTTCAGCGTGGGTGACATTCTAGGTATGTGTATTTAGTT    325

Wildtype         201 AATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACAC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         326 AATCAATTTCCATTTTTTGATATCCTTCAGCTTATTTTGTATCAACACAC    375

Wildtype         251 ATCCAAAGTCTGATTATGTTACTTTCCGTATTTTATACAGTATTCAATTT    300
                     ||||||.|||||||||||||||||||||||||||||||||||||||||||
Mutation         376 ATCCAATGTCTGATTATGTTACTTTCCGTATTTTATACAGTATTCAATTT    425

Wildtype         301 CATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATG    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         426 CATGACCGGCCGACATGACGTGCTGCAAGTAGAAAAAACATCGTTCGATG    475

Wildtype         351 GTTGCAATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCC    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         476 GTTGCAATTCACAGAATGCCATTGGGAGTGCTATAATGACAGGACCAGCC    525

Wildtype         401 AATATGACTCTAAACTCTACCGGAGAACACTACTATATTTGCACTTTTGG    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         526 AATATGACTCTAAACTCTACCGGAGAACACTACTATATTTGCACTTTTGG    575

Wildtype         451 TATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCA    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         576 TATGCACTGCATAAATGGCCAGAAATTAGCTATTACAGTTTCGAACAGCA    625

Wildtype         501 CTGCAACTCCAGGAGCCAACCCTCCTGTTGTACGACAACCAGAAGCTTGT    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         626 CTGCAACTCCAGGAGCCAACCCTCCTGTTGTACGACAACCAGAAGCTTGT    675

Wildtype         551 GCACCTACACCCTCTGCTGCAGGACCTTCA    580
                     ||||||||||||||||||||||||||||||
Mutation         676 GCACCTACACCCTCTGCTGCAGGACCTTCA    705