Plant Crispr Database

Plant Information

Name: CRT0064-3-2

Seed stock: 5432
Seed availability: No

Relationship:
Parent: CRT0064-3

Transformation: CRT0064
transformation CRT0064 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0064-3-2 (T1 )	CRT0064	CTG0064-2	CGR0064-2	gene : Solyc08g075550 (AOX)	-14 bp/-14 bp Is tDNA present: Yes Mutation sequence: AAAATTGTAACAATTGCTATATAAAAGAAATTCCATTACACATCAAAAATAAATAAACAAGATTAGAAATTCGGAGTCTA AAAAAAAATAGTATAATTGTAGAAATTAGACCTCTAAATATAAATTCCTTCAGAAACTCTTTTTCCCTCTCATAAGTTTC TATCAAATCTAATTAATATATATTCATTTAAATGATTGAACAAAAATTGTTCATACTCAATTTTAGTGAACTTATTTAAA AGACCAAAATTAATGAAAAGTATATTGAAACTCCAAAAAAACACAAGGGACCATTACCTACTATAACCCTATACATTATT ATAACGTTTAATTTATATACACTAACAGTATATTTAATTTCGCAGAGACGTCACATGTGTCANGCTATGTTACTAGAAGC CAGGCATGGTAGGTGGTATGCTCCTCCACTGTAAATCCCTCCGTCGATTCGAGCACAGTGGTGGTTGGATCAAAGCCCTC CTCGAAGAAGCAGAGAACGAACGGATGCATCTCANGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 486 TCATGAGATGCATCCGTTCGTTCTCTGCTTCTTCGAGGAGGGCTTTGATC 535 \|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TCNTGAGATGCATCCGTTCGTTCTCTGCTTCTTCGAGGAGGGCTTTGATC 50 Wildtype 536 CAACCACCACTGTGCTCGAATCGACGGAGGGATTTACAGTGGAGGAGCAT 585 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CAACCACCACTGTGCTCGAATCGACGGAGGGATTTACAGTGGAGGAGCAT 100 Wildtype 586 ACCACCTACCATGCCTGGCACGGCTGCAACCGTTTCTAGTAACATAGCAT 635 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\| MutationRev 101 ACCACCTACCATGCCTGGC--------------TTCTAGTAACATAGCNT 136 gRNA GGCACGGCTGCAACCGTTTC Wildtype 636 GACACATGTGACGTCTCTGCGAAATTAAATATACTGTTAGTGTATATAAA 685 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 137 GACACATGTGACGTCTCTGCGAAATTAAATATACTGTTAGTGTATATAAA 186 Wildtype 686 TTAAACGTTATAATAATGTATAGGGTTATAGTAGGTAATGGTCCCTTGTG 735 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 187 TTAAACGTTATAATAATGTATAGGGTTATAGTAGGTAATGGTCCCTTGTG 236 Wildtype 736 TTTTTTTGGAGTTTCAATATACTTTTCATTAATTTTGGTCTTTTAAATAA 785 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 237 TTTTTTTGGAGTTTCAATATACTTTTCATTAATTTTGGTCTTTTAAATAA 286 Wildtype 786 GTTCACTAAAATTGAGTATGAACAATTTTTGTTCAATCATTTAAATGAAT 835 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 287 GTTCACTAAAATTGAGTATGAACAATTTTTGTTCAATCATTTAAATGAAT 336 Wildtype 836 ATATATTAATTAGATTTGATAGAAACTTATGAGAGGGAAAAAGAGTTTCT 885 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 337 ATATATTAATTAGATTTGATAGAAACTTATGAGAGGGAAAAAGAGTTTCT 386 Wildtype 886 GAAGGAATTTATATTTAGAGGTCTAATTTCTACAATTATACTATTTTTTT 935 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 387 GAAGGAATTTATATTTAGAGGTCTAATTTCTACAATTATACTATTTTTTT 436 Wildtype 936 TTAGACTCCGAATTTCTAATCTTGTTTATTTATTTTTGATGTGTAATGGA 985 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 437 TTAGACTCCGAATTTCTAATCTTGTTTATTTATTTTTGATGTGTAATGGA 486 Wildtype 986 ATTTCTTTTATATAGCAATTGTTACAATTTT 1016 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 487 ATTTCTTTTATATAGCAATTGTTACAATTTT 517

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0064-3-2 (T1 )

CRT0064

CTG0064-2

CGR0064-2

gene : Solyc08g075550 (AOX)

-14 bp/-14 bp

Is tDNA present: Yes

Mutation sequence:

AAAATTGTAACAATTGCTATATAAAAGAAATTCCATTACACATCAAAAATAAATAAACAAGATTAGAAATTCGGAGTCTA
AAAAAAAATAGTATAATTGTAGAAATTAGACCTCTAAATATAAATTCCTTCAGAAACTCTTTTTCCCTCTCATAAGTTTC
TATCAAATCTAATTAATATATATTCATTTAAATGATTGAACAAAAATTGTTCATACTCAATTTTAGTGAACTTATTTAAA
AGACCAAAATTAATGAAAAGTATATTGAAACTCCAAAAAAACACAAGGGACCATTACCTACTATAACCCTATACATTATT
ATAACGTTTAATTTATATACACTAACAGTATATTTAATTTCGCAGAGACGTCACATGTGTCANGCTATGTTACTAGAAGC
CAGGCATGGTAGGTGGTATGCTCCTCCACTGTAAATCCCTCCGTCGATTCGAGCACAGTGGTGGTTGGATCAAAGCCCTC
CTCGAAGAAGCAGAGAACGAACGGATGCATCTCANGA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         486 TCATGAGATGCATCCGTTCGTTCTCTGCTTCTTCGAGGAGGGCTTTGATC    535
                     ||.|||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TCNTGAGATGCATCCGTTCGTTCTCTGCTTCTTCGAGGAGGGCTTTGATC     50

Wildtype         536 CAACCACCACTGTGCTCGAATCGACGGAGGGATTTACAGTGGAGGAGCAT    585
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CAACCACCACTGTGCTCGAATCGACGGAGGGATTTACAGTGGAGGAGCAT    100

Wildtype         586 ACCACCTACCATGCCTGGCACGGCTGCAACCGTTTCTAGTAACATAGCAT    635
                     |||||||||||||||||||              |||||||||||||||.|
MutationRev      101 ACCACCTACCATGCCTGGC--------------TTCTAGTAACATAGCNT    136
gRNA                                 GGCACGGCTGCAACCGTTTC

Wildtype         636 GACACATGTGACGTCTCTGCGAAATTAAATATACTGTTAGTGTATATAAA    685
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      137 GACACATGTGACGTCTCTGCGAAATTAAATATACTGTTAGTGTATATAAA    186

Wildtype         686 TTAAACGTTATAATAATGTATAGGGTTATAGTAGGTAATGGTCCCTTGTG    735
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      187 TTAAACGTTATAATAATGTATAGGGTTATAGTAGGTAATGGTCCCTTGTG    236

Wildtype         736 TTTTTTTGGAGTTTCAATATACTTTTCATTAATTTTGGTCTTTTAAATAA    785
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      237 TTTTTTTGGAGTTTCAATATACTTTTCATTAATTTTGGTCTTTTAAATAA    286

Wildtype         786 GTTCACTAAAATTGAGTATGAACAATTTTTGTTCAATCATTTAAATGAAT    835
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      287 GTTCACTAAAATTGAGTATGAACAATTTTTGTTCAATCATTTAAATGAAT    336

Wildtype         836 ATATATTAATTAGATTTGATAGAAACTTATGAGAGGGAAAAAGAGTTTCT    885
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      337 ATATATTAATTAGATTTGATAGAAACTTATGAGAGGGAAAAAGAGTTTCT    386

Wildtype         886 GAAGGAATTTATATTTAGAGGTCTAATTTCTACAATTATACTATTTTTTT    935
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      387 GAAGGAATTTATATTTAGAGGTCTAATTTCTACAATTATACTATTTTTTT    436

Wildtype         936 TTAGACTCCGAATTTCTAATCTTGTTTATTTATTTTTGATGTGTAATGGA    985
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      437 TTAGACTCCGAATTTCTAATCTTGTTTATTTATTTTTGATGTGTAATGGA    486

Wildtype         986 ATTTCTTTTATATAGCAATTGTTACAATTTT   1016
                     |||||||||||||||||||||||||||||||
MutationRev      487 ATTTCTTTTATATAGCAATTGTTACAATTTT    517