Plant Crispr Database

Plant Information

Name: CRT0085-1-6

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0085-1

Transformation: CRT0085
transformation CRT0085 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0085-1-6 (T1 )	CRT0085	CTG0085-1	CGR0085-1	gene : Solyc08g081210 (MKKK66)	+1 bp/+1 bp Is tDNA present: Yes Mutation sequence: AAGGATGTAAGGAGGAAATCCACTAAGGAGAGTTTCATTGACATAATAAATCGGAAACTGAAGATTTTCACCACGGAAAA ATCAAGTGGTAAATCTGGATCATCTCGAAGACGACGTAAAGATAAAAATTCAGTGAAGGGTTCTCAATCAAGGGTTTCAA GGTCACCATCACCATCTACGCCAGATTCACGCAAGTCAGGTCTTTGCTGATAGGACTTCTTCTCAACCACTTCCCCTTCC TGAGGGTCACTCTTCTAATGTACATCTTGTCGACTCTGATAACAGTGGATCCATAATATTAGTGACCGGTGAAGTCTCCG AGCCATCATTGACTTTGCCTCTTCCCATGCCCAGGCATCTTCCACATGGACCAACTGCTGCAGGAGTTGACAGGGACTTA CCAACTGCTTCTGTTTCTTGTGACTGCTCCAGTGACAGTGATGATCTTACTGACTCACGATTTCTAAGTCCCCAAACATC TGATTATGAAAACGGGAGCAGAACTGCCTTGAATAGTCCTTCCAGGTAAGCACAAACTGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 5881 ACAGTTTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTT 5930 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ACAGTTTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTT 50 Wildtype 5931 TTCATAATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCAT 5980 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TTCATAATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCAT 100 Wildtype 5981 CACTGTCACTGGAGCTGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTG 6030 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CACTGTCACTGGAGCAGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTG 150 Wildtype 6031 TCAACTCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAG 6080 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TCAACTCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAG 200 Wildtype 6081 AGGCAAAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGG 6130 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 AGGCAAAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGG 250 Wildtype 6131 ATCCACTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCA 6180 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 ATCCACTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCA 300 Wildtype 6181 GGAAGGGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGAC-TGC 6229 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| MutationRev 301 GGAAGGGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGACTTGC 350 gRNA GAC-TGC Wildtype 6230 GTGAATCTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAA 6279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GTGAATCTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAA 400 gRNA GACTGCGTGAATCTGGCGT Wildtype 6280 CCCTTCACTGAATTTGTATCTTTACGTCGTCTTCGAGATGATCCAGATTT 6329 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 CCCTTCACTGAATTTTTATCTTTACGTCGTCTTCGAGATGATCCAGATTT 450 Wildtype 6330 ACCACTTGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGT 6379 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 ACCACTTGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGT 500 Wildtype 6380 CAATGAAACTCTCCTTAGTGGATTTCCTCCTTACATCCTT 6419 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 CAATGAAACTCTCCTTAGTGGATTTCCTCCTTACATCCTT 540

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0085-1-6 (T1 )

CRT0085

CTG0085-1

CGR0085-1

gene : Solyc08g081210 (MKKK66)

+1 bp/+1 bp

Is tDNA present: Yes

Mutation sequence:

AAGGATGTAAGGAGGAAATCCACTAAGGAGAGTTTCATTGACATAATAAATCGGAAACTGAAGATTTTCACCACGGAAAA
ATCAAGTGGTAAATCTGGATCATCTCGAAGACGACGTAAAGATAAAAATTCAGTGAAGGGTTCTCAATCAAGGGTTTCAA
GGTCACCATCACCATCTACGCCAGATTCACGCAAGTCAGGTCTTTGCTGATAGGACTTCTTCTCAACCACTTCCCCTTCC
TGAGGGTCACTCTTCTAATGTACATCTTGTCGACTCTGATAACAGTGGATCCATAATATTAGTGACCGGTGAAGTCTCCG
AGCCATCATTGACTTTGCCTCTTCCCATGCCCAGGCATCTTCCACATGGACCAACTGCTGCAGGAGTTGACAGGGACTTA
CCAACTGCTTCTGTTTCTTGTGACTGCTCCAGTGACAGTGATGATCTTACTGACTCACGATTTCTAAGTCCCCAAACATC
TGATTATGAAAACGGGAGCAGAACTGCCTTGAATAGTCCTTCCAGGTAAGCACAAACTGT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        5881 ACAGTTTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTT   5930
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 ACAGTTTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTT     50

Wildtype        5931 TTCATAATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCAT   5980
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TTCATAATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCAT    100

Wildtype        5981 CACTGTCACTGGAGCTGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTG   6030
                     |||||||||||||||.||||||||||||||||||||||||||||||||||
MutationRev      101 CACTGTCACTGGAGCAGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTG    150

Wildtype        6031 TCAACTCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAG   6080
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 TCAACTCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAG    200

Wildtype        6081 AGGCAAAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGG   6130
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 AGGCAAAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGG    250

Wildtype        6131 ATCCACTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCA   6180
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 ATCCACTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCA    300

Wildtype        6181 GGAAGGGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGAC-TGC   6229
                     |||||||||||||||||||||||||||||||||||||||||||||| |||
MutationRev      301 GGAAGGGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGACTTGC    350
gRNA                                                            GAC-TGC

Wildtype        6230 GTGAATCTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAA   6279
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 GTGAATCTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAA    400
gRNA                 GACTGCGTGAATCTGGCGT

Wildtype        6280 CCCTTCACTGAATTTGTATCTTTACGTCGTCTTCGAGATGATCCAGATTT   6329
                     |||||||||||||||.||||||||||||||||||||||||||||||||||
MutationRev      401 CCCTTCACTGAATTTTTATCTTTACGTCGTCTTCGAGATGATCCAGATTT    450

Wildtype        6330 ACCACTTGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGT   6379
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 ACCACTTGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGT    500

Wildtype        6380 CAATGAAACTCTCCTTAGTGGATTTCCTCCTTACATCCTT   6419
                     ||||||||||||||||||||||||||||||||||||||||
MutationRev      501 CAATGAAACTCTCCTTAGTGGATTTCCTCCTTACATCCTT    540