Plant Crispr Database

Plant Information

Name: CRT0094-1-2

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0094-1

Transformation: CRT0094
transformation CRT0094 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0094-1-2 (T1 )	CRT0094	CTG0094-1	CGR0094-1	gene : Solyc12g009020 (MKK1)	-2 bp/-2 bp Is tDNA present: No Mutation sequence: TCACATTTATGCTAAANTCATCTGTACTTTGTGTCAACTGACTTGAACTGTTGGATCCACTTCTTGTATCTCTTATTTCT GTGTTTCTCTAGCTATGGTTATGTATTTTGATGAGATGTTTTTGGTATTTTGTTGTATAGGACTGAATCAGGAACTTAAG GATGGAGATCTTCTGGTGAATAGAGATGGAGTTCGAATTGTTTCGCAGAGTGAAGTTGCAGCTGTACACTTTCTCTTTTG AATTACTTATGTTGTATTTGATTAAAGGAAATGTTGGTTTGATTTGTTTGAATTGCTTTTATAGTTCTGTAGTCTTGCTT GGATTGGGTAGTAGGATGATCCTTTGCTTAGTTTACATGGGATTAAGAGTAGACTATTCTCTAATTTGCCTTTGGAAGTT TGAAGTTTGAATTGGAATGTTTAGCTGACCTGAGATAGGAGGGTATGGATGTTGCGGATTAGGGTAGAAGGTAAGTAGGT AATTGAGCTTTGACTCATTTTTCGACGGGTTTAAGATTGGTGGGTGTCGGGGAACACTGTGTCTGTTGTAGTATTAGNCT CATACATGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 3213 ACATGTATGAGACTAATACTACAACAGACACAGTGTTCCCCGACACCCAC 3262 \|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ACATGTATGAGNCTAATACTACAACAGACACAGTGTTCCCCGACACCCAC 50 Wildtype 3263 CAATCTTAAACCCGTCGAAAAATGAGTCAAAGCTCAATTACCTACTTACC 3312 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CAATCTTAAACCCGTCGAAAAATGAGTCAAAGCTCAATTACCTACTTACC 100 Wildtype 3313 TTCTACCCTAATCCGCAACATCCATACCCTCCTATCTCAGGTCAGCTAAA 3362 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TTCTACCCTAATCCGCAACATCCATACCCTCCTATCTCAGGTCAGCTAAA 150 Wildtype 3363 CATTCCAATTCAAACTTCAAACTTCCAAAGGCAAATTAGAGAATAGTCTA 3412 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 CATTCCAATTCAAACTTCAAACTTCCAAAGGCAAATTAGAGAATAGTCTA 200 Wildtype 3413 CTCTTAATCCCATGTAAACTAAGCAAAGGATCATCCTACTACCCAATCCA 3462 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 CTCTTAATCCCATGTAAACTAAGCAAAGGATCATCCTACTACCCAATCCA 250 Wildtype 3463 AGCAAGACTACAGAACTATAAAAGCAATTCAAACAAATCAAACCAACATT 3512 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 AGCAAGACTACAGAACTATAAAAGCAATTCAAACAAATCAAACCAACATT 300 Wildtype 3513 TCCTTTAATCAAATACAACATAAGTAATTCAAAAGAGAAAGTGTACAGCT 3562 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 TCCTTTAATCAAATACAACATAAGTAATTCAAAAGAGAAAGTGTACAGCT 350 Wildtype 3563 GCAACTTCACTCTGCGAAACAATTCGAACTCCATCTCTATTCACCAGAAG 3612 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GCAACTTCACTCTGCGAAACAATTCGAACTCCATCTCTATTCACCAGAAG 400 Wildtype 3613 ATCTCCATCCTTAAATGTTCCTGATTCAGTCCTATACAACAAAATACCAA 3662 \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 ATCTCCATCCTTAA--GTTCCTGATTCAGTCCTATACAACAAAATACCAA 448 gRNA TAAATGTTCCTGATTCAGTC Wildtype 3663 AAACATCTCATCAAAATACATAACCATAGCTAGAGAAACACAGAAATAAG 3712 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 449 AAACATCTCATCAAAATACATAACCATAGCTAGAGAAACACAGAAATAAG 498 Wildtype 3713 AGATACAAGAAGTGGATCCAACAGTTCAAGTCAGTTGACACAAAGTACAG 3762 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 499 AGATACAAGAAGTGGATCCAACAGTTCAAGTCAGTTGACACAAAGTACAG 548 Wildtype 3763 ATGAATTTAGCATAAATGTGA 3783 \|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 549 ATGANTTTAGCATAAATGTGA 569	Immunity response: More susceptible to DC3000dd

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0094-1-2 (T1 )

CRT0094

CTG0094-1

CGR0094-1

gene : Solyc12g009020 (MKK1)

-2 bp/-2 bp

Is tDNA present: No

Mutation sequence:

TCACATTTATGCTAAANTCATCTGTACTTTGTGTCAACTGACTTGAACTGTTGGATCCACTTCTTGTATCTCTTATTTCT
GTGTTTCTCTAGCTATGGTTATGTATTTTGATGAGATGTTTTTGGTATTTTGTTGTATAGGACTGAATCAGGAACTTAAG
GATGGAGATCTTCTGGTGAATAGAGATGGAGTTCGAATTGTTTCGCAGAGTGAAGTTGCAGCTGTACACTTTCTCTTTTG
AATTACTTATGTTGTATTTGATTAAAGGAAATGTTGGTTTGATTTGTTTGAATTGCTTTTATAGTTCTGTAGTCTTGCTT
GGATTGGGTAGTAGGATGATCCTTTGCTTAGTTTACATGGGATTAAGAGTAGACTATTCTCTAATTTGCCTTTGGAAGTT
TGAAGTTTGAATTGGAATGTTTAGCTGACCTGAGATAGGAGGGTATGGATGTTGCGGATTAGGGTAGAAGGTAAGTAGGT
AATTGAGCTTTGACTCATTTTTCGACGGGTTTAAGATTGGTGGGTGTCGGGGAACACTGTGTCTGTTGTAGTATTAGNCT
CATACATGT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        3213 ACATGTATGAGACTAATACTACAACAGACACAGTGTTCCCCGACACCCAC   3262
                     |||||||||||.||||||||||||||||||||||||||||||||||||||
MutationRev        1 ACATGTATGAGNCTAATACTACAACAGACACAGTGTTCCCCGACACCCAC     50

Wildtype        3263 CAATCTTAAACCCGTCGAAAAATGAGTCAAAGCTCAATTACCTACTTACC   3312
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CAATCTTAAACCCGTCGAAAAATGAGTCAAAGCTCAATTACCTACTTACC    100

Wildtype        3313 TTCTACCCTAATCCGCAACATCCATACCCTCCTATCTCAGGTCAGCTAAA   3362
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 TTCTACCCTAATCCGCAACATCCATACCCTCCTATCTCAGGTCAGCTAAA    150

Wildtype        3363 CATTCCAATTCAAACTTCAAACTTCCAAAGGCAAATTAGAGAATAGTCTA   3412
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 CATTCCAATTCAAACTTCAAACTTCCAAAGGCAAATTAGAGAATAGTCTA    200

Wildtype        3413 CTCTTAATCCCATGTAAACTAAGCAAAGGATCATCCTACTACCCAATCCA   3462
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 CTCTTAATCCCATGTAAACTAAGCAAAGGATCATCCTACTACCCAATCCA    250

Wildtype        3463 AGCAAGACTACAGAACTATAAAAGCAATTCAAACAAATCAAACCAACATT   3512
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 AGCAAGACTACAGAACTATAAAAGCAATTCAAACAAATCAAACCAACATT    300

Wildtype        3513 TCCTTTAATCAAATACAACATAAGTAATTCAAAAGAGAAAGTGTACAGCT   3562
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 TCCTTTAATCAAATACAACATAAGTAATTCAAAAGAGAAAGTGTACAGCT    350

Wildtype        3563 GCAACTTCACTCTGCGAAACAATTCGAACTCCATCTCTATTCACCAGAAG   3612
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 GCAACTTCACTCTGCGAAACAATTCGAACTCCATCTCTATTCACCAGAAG    400

Wildtype        3613 ATCTCCATCCTTAAATGTTCCTGATTCAGTCCTATACAACAAAATACCAA   3662
                     ||||||||||||||  ||||||||||||||||||||||||||||||||||
MutationRev      401 ATCTCCATCCTTAA--GTTCCTGATTCAGTCCTATACAACAAAATACCAA    448
gRNA                            TAAATGTTCCTGATTCAGTC

Wildtype        3663 AAACATCTCATCAAAATACATAACCATAGCTAGAGAAACACAGAAATAAG   3712
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      449 AAACATCTCATCAAAATACATAACCATAGCTAGAGAAACACAGAAATAAG    498

Wildtype        3713 AGATACAAGAAGTGGATCCAACAGTTCAAGTCAGTTGACACAAAGTACAG   3762
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      499 AGATACAAGAAGTGGATCCAACAGTTCAAGTCAGTTGACACAAAGTACAG    548

Wildtype        3763 ATGAATTTAGCATAAATGTGA   3783
                     ||||.||||||||||||||||
MutationRev      549 ATGANTTTAGCATAAATGTGA    569

Immunity response: More susceptible to DC3000dd