Plant Crispr Database

Plant Information

Name: CRT0103-1-16

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0103-1

Transformation: CRT0103
transformation CRT0103 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0103-1-16 (T1 )	CRT0103	CTG0103-1	CGR0103-1	gene : Solyc03g093560 (ERF5)	-7 bp /-7 bp Is tDNA present: Yes Mutation sequence: ACTTCAAATCTTGATGACATCGCAAGCTTCTTTAATGCTACTTCAAAAACAGAGTATGATGGTTTTTTCGAATTTGAGGC AAAACGACATGTTATCCATTCAAATTCTCCGAAACAGAGTAACTTGAGAGAACGGAAGCCATCTTAGCAATACCGGCGAA GCCTGTTGTTGTTGTAGAGAACGTTGAGATTGAGAAGAAGCATTACAGGGGAGTTAGACAGAGGCCATGGGGGAAGTTTG CAGCGGAGATTCGTGACCCAAATAGAAAGGGGACTCGAGTTTGGTTAGGAACATTTGATACTGCTGTGGATGCGGCAAAG GCATATGACAGGGCAGCGTTTAAGCTTAGAGGAAGCAAAGCAATATTGAATTTCCCACTCGAAGTTGCAAACTTTAAGCA ACAAAATGATGAGACTAAAACAGAGATGAAGTCGTCAGGCAGTAAAAGGGTGAGAGGAGAAACAGAGGAATTAGTAATCA AGAAGGAAAGGAAAATAGAAGAAGAAAGAGTACTCCCAACGGCGGCGGCTCCATTAACACCGTCAAGTTGGTCGACGATT TGGGATGAAAAGGGTATTTTTGAGGTGCCACCATTGTCACCATTATCTCAGCTTGTTATGATATAATTTTGGATTAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1 TTATATCATAACAAGCTGAGATAATGGTGACAATGGTGGCACCTCAAAAA 50 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 12 TTATATCATAACAAGCTGAGATAATGGTGACAATGGTGGCACCTCAAAAA 61 Wildtype 51 TACCCTTTTCATCCCAAATCGTCGACCAACTTGACGGTGTTAATGGAGCC 100 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 62 TACCCTTTTCATCCCAAATCGTCGACCAACTTGACGGTGTTAATGGAGCC 111 Wildtype 101 GCCGCCGTTGGGAGTACTCTTTCTTCTTCTATTTTCCTTTCCTTCTTGAT 150 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 112 GCCGCCGTTGGGAGTACTCTTTCTTCTTCTATTTTCCTTTCCTTCTTGAT 161 Wildtype 151 TACTAATTCCTCTGTTTCTCCTCTCACCCTTTTACTGCCTGACGACTTCA 200 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 162 TACTAATTCCTCTGTTTCTCCTCTCACCCTTTTACTGCCTGACGACTTCA 211 Wildtype 201 TCTCTGTTTTAGTCTCATCATTTTGTTGCTTAAAGTTTGCAACTTCGAGT 250 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 212 TCTCTGTTTTAGTCTCATCATTTTGTTGCTTAAAGTTTGCAACTTCGAGT 261 Wildtype 251 GGGAAATTCAATATTGCTTTGCTTCCTCTAAGCTTAAACGCTGCCCTGTC 300 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 262 GGGAAATTCAATATTGCTTTGCTTCCTCTAAGCTTAAACGCTGCCCTGTC 311 Wildtype 301 ATATGCCTTTGCCGCATCCACAGCAGTATCAAATGTTCCTAACCAAACTC 350 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 312 ATATGCCTTTGCCGCATCCACAGCAGTATCAAATGTTCCTAACCAAACTC 361 Wildtype 351 GAGTCCCCTTTCTATTTGGGTCACGAATCTCCGCTGCAAACTTCCCCCAT 400 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 362 GAGTCCCCTTTCTATTTGGGTCACGAATCTCCGCTGCAAACTTCCCCCAT 411 Wildtype 401 GGCCTCTGTCTAACTCCCCTGTAATGCTTCTTCTCAATCTCAACGTTCTC 450 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 412 GGCCTCTGTCTAACTCCCCTGTAATGCTTCTTCTCAATCTCAACGTTCTC 461 Wildtype 451 TACAACAACAACAGGCTTCGCCGGTATTGCTACGTTCAGAGATGGCTTCC 500 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\| MutationRev 462 TACAACAACAACAGGCTTCGCCGGTATTGCTA-------AGATGGCTTCC 504 gRNA GGTATTGCTACGTTCAGAGA Wildtype 501 GTTCTCTCAAGTTACTCTGTTTCGGAGAATTTGAATGGATAACATGTCGT 550 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 505 GTTCTCTCAAGTTACTCTGTTTCGGAGAATTTGAATGGATAACATGTCGT 554 Wildtype 551 TTTGCCTCAAATTCGAAAAAACCATCATACTCTGTTTTTGAAGTAGCATT 600 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 555 TTTGCCTCAAATTCGAAAAAACCATCATACTCTGTTTTTGAAGTAGCATT 604 Wildtype 601 AAAGAAGCTTGCGATGTCATCAAGATTTGAAGT 633 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 605 AAAGAAGCTTGCGATGTCATCAAGATTTGAAGT 637

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0103-1-16 (T1 )

CRT0103

CTG0103-1

CGR0103-1

gene : Solyc03g093560 (ERF5)

-7 bp /-7 bp

Is tDNA present: Yes

Mutation sequence:

ACTTCAAATCTTGATGACATCGCAAGCTTCTTTAATGCTACTTCAAAAACAGAGTATGATGGTTTTTTCGAATTTGAGGC
AAAACGACATGTTATCCATTCAAATTCTCCGAAACAGAGTAACTTGAGAGAACGGAAGCCATCTTAGCAATACCGGCGAA
GCCTGTTGTTGTTGTAGAGAACGTTGAGATTGAGAAGAAGCATTACAGGGGAGTTAGACAGAGGCCATGGGGGAAGTTTG
CAGCGGAGATTCGTGACCCAAATAGAAAGGGGACTCGAGTTTGGTTAGGAACATTTGATACTGCTGTGGATGCGGCAAAG
GCATATGACAGGGCAGCGTTTAAGCTTAGAGGAAGCAAAGCAATATTGAATTTCCCACTCGAAGTTGCAAACTTTAAGCA
ACAAAATGATGAGACTAAAACAGAGATGAAGTCGTCAGGCAGTAAAAGGGTGAGAGGAGAAACAGAGGAATTAGTAATCA
AGAAGGAAAGGAAAATAGAAGAAGAAAGAGTACTCCCAACGGCGGCGGCTCCATTAACACCGTCAAGTTGGTCGACGATT
TGGGATGAAAAGGGTATTTTTGAGGTGCCACCATTGTCACCATTATCTCAGCTTGTTATGATATAATTTTGGATTAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 TTATATCATAACAAGCTGAGATAATGGTGACAATGGTGGCACCTCAAAAA     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       12 TTATATCATAACAAGCTGAGATAATGGTGACAATGGTGGCACCTCAAAAA     61

Wildtype          51 TACCCTTTTCATCCCAAATCGTCGACCAACTTGACGGTGTTAATGGAGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       62 TACCCTTTTCATCCCAAATCGTCGACCAACTTGACGGTGTTAATGGAGCC    111

Wildtype         101 GCCGCCGTTGGGAGTACTCTTTCTTCTTCTATTTTCCTTTCCTTCTTGAT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      112 GCCGCCGTTGGGAGTACTCTTTCTTCTTCTATTTTCCTTTCCTTCTTGAT    161

Wildtype         151 TACTAATTCCTCTGTTTCTCCTCTCACCCTTTTACTGCCTGACGACTTCA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      162 TACTAATTCCTCTGTTTCTCCTCTCACCCTTTTACTGCCTGACGACTTCA    211

Wildtype         201 TCTCTGTTTTAGTCTCATCATTTTGTTGCTTAAAGTTTGCAACTTCGAGT    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      212 TCTCTGTTTTAGTCTCATCATTTTGTTGCTTAAAGTTTGCAACTTCGAGT    261

Wildtype         251 GGGAAATTCAATATTGCTTTGCTTCCTCTAAGCTTAAACGCTGCCCTGTC    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      262 GGGAAATTCAATATTGCTTTGCTTCCTCTAAGCTTAAACGCTGCCCTGTC    311

Wildtype         301 ATATGCCTTTGCCGCATCCACAGCAGTATCAAATGTTCCTAACCAAACTC    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      312 ATATGCCTTTGCCGCATCCACAGCAGTATCAAATGTTCCTAACCAAACTC    361

Wildtype         351 GAGTCCCCTTTCTATTTGGGTCACGAATCTCCGCTGCAAACTTCCCCCAT    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      362 GAGTCCCCTTTCTATTTGGGTCACGAATCTCCGCTGCAAACTTCCCCCAT    411

Wildtype         401 GGCCTCTGTCTAACTCCCCTGTAATGCTTCTTCTCAATCTCAACGTTCTC    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      412 GGCCTCTGTCTAACTCCCCTGTAATGCTTCTTCTCAATCTCAACGTTCTC    461

Wildtype         451 TACAACAACAACAGGCTTCGCCGGTATTGCTACGTTCAGAGATGGCTTCC    500
                     ||||||||||||||||||||||||||||||||       |||||||||||
MutationRev      462 TACAACAACAACAGGCTTCGCCGGTATTGCTA-------AGATGGCTTCC    504
gRNA                                       GGTATTGCTACGTTCAGAGA

Wildtype         501 GTTCTCTCAAGTTACTCTGTTTCGGAGAATTTGAATGGATAACATGTCGT    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      505 GTTCTCTCAAGTTACTCTGTTTCGGAGAATTTGAATGGATAACATGTCGT    554

Wildtype         551 TTTGCCTCAAATTCGAAAAAACCATCATACTCTGTTTTTGAAGTAGCATT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      555 TTTGCCTCAAATTCGAAAAAACCATCATACTCTGTTTTTGAAGTAGCATT    604

Wildtype         601 AAAGAAGCTTGCGATGTCATCAAGATTTGAAGT    633
                     |||||||||||||||||||||||||||||||||
MutationRev      605 AAAGAAGCTTGCGATGTCATCAAGATTTGAAGT    637