Is tDNA present: Yes

Mutation sequence:

GCTTGTTCTAGCTGGATATCTCATGGTGATTATCATACTTCAAAAGAAATTTAATTTCACACAGATGGAGTACGGTTTGA
GTGCAGCTCTAGTGGTATTCTTGCTTTTCCTACCACTTGGTTTGGTTATAAAAGAGGAAATCAATTCATGGAACGCGAAG
AAATTAGCCTTGGATAGTATTTCCAAGGTCAAAGTTGTAACTGAAAACCCTAAAAGTGAGGGTCCTCAATTACCACTGCC
AGCACCAGCATCAGCAGCAGCATCGGTTATTACTGACACGACTTTTGAGAAACAAGATTTGGATGGTATTGTTGAGGGGA
AGTCAAAAAGTAGTTTTGATGTTGACTTCAAAGATTCAGAAGTTTCGATTTCTTGCTGGAAGACGGTTTTTCAACCGCCA
GCTTCGGGGCGAAGACTTTACTATACTCCAAGCTCTATTCAGTCTAGACATGTTGATACTATTTGTTGCTACAATTTGTG
GAGTTGGAGGGACATTAACAGCTATAGACAACTTAGGTCAAATTGGGACATCACTAGGGTACCCTAAAAAAAGTATTAGT
ACATTTGTGTCCTTAGTAAGTATATGGAATTATTTAGGTAGAGTAGTGGCTGGTTTTTTATCTGAATATTTTTTGAAAAA
ATACAAATTTCCAAGACCTTTAGCCCTAACCATAACACTAATAGTCTCATGCATAGGACACATTTTAATTGCATTTAATG
T

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         505 GGTTAGGGCTAAAGGTCTTGGAAATTTGTATTTTTTCAAAAAATATTCAG    554
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 GGTTAGGGCTAAAGGTCTTGGAAATTTGTATTTTTTCAAAAAATATTCAG     50

Wildtype         555 ATAAAAAACCAGCCACTACTCTACCTAAATAATTCCATATACTTACTAAG    604
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATAAAAAACCAGCCACTACTCTACCTAAATAATTCCATATACTTACTAAG    100

Wildtype         605 GACACAAATGTACTAATACTTTTTTTAGGGTACCCTAGTGATGTCCCAAT    654
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 GACACAAATGTACTAATACTTTTTTTAGGGTACCCTAGTGATGTCCCAAT    150

Wildtype         655 TTGACCTAAGTTGTCTATAGCTGTTAATGTCCCTCCAACTCCACAAATTG    704
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TTGACCTAAGTTGTCTATAGCTGTTAATGTCCCTCCAACTCCACAAATTG    200

Wildtype         705 TAGCAACAAATAGTATCAACATGTCTAGACTGAATAGAGCTTGGAGTATA    754
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TAGCAACAAATAGTATCAACATGTCTAGACTGAATAGAGCTTGGAGTATA    250

Wildtype         755 GTAAAGTCTTCGCCCCG-AGCTGGCGGTTGAAAAACCGTCTTCCAGCAAG    803
                     ||||||||||||||||| ||||||||||||||||||||||||||||||||
Mutation         251 GTAAAGTCTTCGCCCCGAAGCTGGCGGTTGAAAAACCGTCTTCCAGCAAG    300
gRNA                 GTAAAGTCTTCGCCCCG-AGC

Wildtype         804 AAATCGAAACTTCTGAATCTTTGAAGTCAACATCAAAACTACTTTTTGAC    853
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 AAATCGAAACTTCTGAATCTTTGAAGTCAACATCAAAACTACTTTTTGAC    350

Wildtype         854 TTCCCCTCAACAATACCATCCAAATCTTGTTTCTCAAAA    892
                     |||||||||||||||||||||||||||||||||||||||
Mutation         351 TTCCCCTCAACAATACCATCCAAATCTTGTTTCTCAAAA    389