Plant Crispr Database

Plant Information

Name: CRT0119-1-2

Seed stock:
Seed availability: No

Relationship:
Parent: CRT0119-1

Transformation: CRT0119
transformation CRT0119 to RG-PtoR

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0119-1-2 (T1 )	CRT0119	CTG0119-2	CGR0119-2	gene : Solyc08g006320 (WRKY11)	-4 bp/-4 bp Is tDNA present: No Mutation sequence: CTGCAAATCAAACAACAAAAATGGCTGTAGATTTGTTAAATTATTCGAATTTGAATGAACAATTAGCTTTACAAGAAGCT GCTACAGCCGGTTTAAAATCTATGGATAATTTAATCCGGTTCGTTTCATTTCAACAACAGCAAAATCAAACGGTTCAGCC GGATTGTAGAGAGATAACTGATTATACTGTTTCGAATTTTAGAAAGGTTATTACTATTTTGAACCGGACCGGTCATGCCC GGTTTAGACGTAGTCCGGTTCAGGTTACTGATGATCTACGGCTTTAACTCTATCGCCGTTGACAAATCCGGCGGAGGAAA CTGTACCGGCGGTGAAAGTACCGGTTGAGAAGTATCAATCGAAGGCGTTGACTTTAGATTTTACGAAACGGAAAGTTGGT AAATCTATTGGATGTGAAGCTGTTCCGGTAGCGAGTTCGACGACGTCGTCGTCGTTTATGTCGACGATTACAGGTGAAGG AAGTGTTTCGAATGGTAAAGTGTTTTCGTCGATGAGTTTACCTCCTCGTCCGCCTGTTTCCTCCGGGAAGCCGCCAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 896 ATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGTAAACTCATCGA 945 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGTAAACTCATCGA 50 Wildtype 946 CGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGTAATCGTCGACA 995 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGTAATCGTCGACA 100 Wildtype 996 TAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAGCTTCACATCCA 1045 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAGCTTCACATCCA 150 Wildtype 1046 ATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTCAACGCCTTCGA 1095 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 ATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTCAACGCCTTCGA 200 Wildtype 1096 TTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGTTTCCTCCGCCG 1145 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGTTTCCTCCGCCG 250 Wildtype 1146 GATTTGTCAACGGCGATAGAGTTAAAGCCGTAGACGAATCATCAGTAACC 1195 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GATTTGTCAACGGCGATAGAGTTAAAGCCGTAG----ATCATCAGTAACC 296 gRNA TAGACGAATCATCAGTAACC Wildtype 1196 TGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGGTTCAAAATAGT 1245 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 297 TGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGGTTCAAAATAGT 346 Wildtype 1246 AATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTATCTCTCTACAAT 1295 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 347 AATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTATCTCTCTACAAT 396 Wildtype 1296 CCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAACGAACCGGATT 1345 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 397 CCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAACGAACCGGATT 446 Wildtype 1346 AAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCTTGTAAAGCTAA 1395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 447 AAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCTTGTAAAGCTAA 496 Wildtype 1396 TTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGCCATTTTTGTTG 1445 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 497 TTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGCCATTTTTGTTG 546 Wildtype 1446 TTTGATTTGCAG 1457 \|\|\|\|\|\|\|\|\|\|\|\| MutationRev 547 TTTGATTTGCAG 558

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0119-1-2 (T1 )

CRT0119

CTG0119-2

CGR0119-2

gene : Solyc08g006320 (WRKY11)

-4 bp/-4 bp

Is tDNA present: No

Mutation sequence:

CTGCAAATCAAACAACAAAAATGGCTGTAGATTTGTTAAATTATTCGAATTTGAATGAACAATTAGCTTTACAAGAAGCT
GCTACAGCCGGTTTAAAATCTATGGATAATTTAATCCGGTTCGTTTCATTTCAACAACAGCAAAATCAAACGGTTCAGCC
GGATTGTAGAGAGATAACTGATTATACTGTTTCGAATTTTAGAAAGGTTATTACTATTTTGAACCGGACCGGTCATGCCC
GGTTTAGACGTAGTCCGGTTCAGGTTACTGATGATCTACGGCTTTAACTCTATCGCCGTTGACAAATCCGGCGGAGGAAA
CTGTACCGGCGGTGAAAGTACCGGTTGAGAAGTATCAATCGAAGGCGTTGACTTTAGATTTTACGAAACGGAAAGTTGGT
AAATCTATTGGATGTGAAGCTGTTCCGGTAGCGAGTTCGACGACGTCGTCGTCGTTTATGTCGACGATTACAGGTGAAGG
AAGTGTTTCGAATGGTAAAGTGTTTTCGTCGATGAGTTTACCTCCTCGTCCGCCTGTTTCCTCCGGGAAGCCGCCAAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         896 ATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGTAAACTCATCGA    945
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 ATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGTAAACTCATCGA     50

Wildtype         946 CGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGTAATCGTCGACA    995
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGTAATCGTCGACA    100

Wildtype         996 TAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAGCTTCACATCCA   1045
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 TAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAGCTTCACATCCA    150

Wildtype        1046 ATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTCAACGCCTTCGA   1095
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 ATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTCAACGCCTTCGA    200

Wildtype        1096 TTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGTTTCCTCCGCCG   1145
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 TTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGTTTCCTCCGCCG    250

Wildtype        1146 GATTTGTCAACGGCGATAGAGTTAAAGCCGTAGACGAATCATCAGTAACC   1195
                     |||||||||||||||||||||||||||||||||    |||||||||||||
MutationRev      251 GATTTGTCAACGGCGATAGAGTTAAAGCCGTAG----ATCATCAGTAACC    296
gRNA                                               TAGACGAATCATCAGTAACC

Wildtype        1196 TGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGGTTCAAAATAGT   1245
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      297 TGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGGTTCAAAATAGT    346

Wildtype        1246 AATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTATCTCTCTACAAT   1295
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      347 AATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTATCTCTCTACAAT    396

Wildtype        1296 CCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAACGAACCGGATT   1345
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      397 CCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAACGAACCGGATT    446

Wildtype        1346 AAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCTTGTAAAGCTAA   1395
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      447 AAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCTTGTAAAGCTAA    496

Wildtype        1396 TTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGCCATTTTTGTTG   1445
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      497 TTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGCCATTTTTGTTG    546

Wildtype        1446 TTTGATTTGCAG   1457
                     ||||||||||||
MutationRev      547 TTTGATTTGCAG    558