Plant Crispr Database

Plant Information

Name: CRT0137-11-8

Seed stock: 4781
Seed availability: No

Relationship:
Parent: CRT0137-11

Transformation: CRT0137
transformation CRT0137 to M82 sp+

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0137-11-8 (T1 )	CRT0137	CTG0137-21	CGR0137-21	gene : Solyc04g014650 (LRRXII-RLK)	-2 bp/+1 bp	Morphology : "Stunted, bleached plants"
CRT0137-11-8 (T1 )	CRT0137	CTG0137-21	CGR0137-21	gene : Solyc04g014890 (LRRXII-RLK)	+1 bp/+1 bp Is tDNA present: No Mutation sequence: ACAACGATTTAATATTTTTTAATTTTTTTTTAAATCGCTGCCCCTACAGCGAGATTCAACGAGCAACAAATAATTTTGAT GAATCCAATTTAATTGGTGAGGGAAGCTCTGGCTCTGTGTACAAAGGTGCATTTGTTTGGTGGAACTGCAGTGGCCATTA AGGTTCTGGATTTGGAAAATGAGCAAGTATGCAAGAGGTTTGATACCGAATGTGAAGTGATGAGATATGTTAGACACAGA AATCTTGTTTCTGTGGTTACTACGTGTTCTAGTGACTATATAAGAGCCTTTGTTCTGCATTTTATGCCCAATGGAAGTCT TGAGAATTGGTTGTACAAAGAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 468 CTCTTTGTACAACCAATTCTCAAGACTTCCATTGGGCATAAAATGCAGAA 517 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 CTCTTTGTACAACCAATTCTCAAGACTTCCATTGGGCATAAAATGCAGAA 50 Wildtype 518 CAAAGGCTCTTATATAGTCACTAGAACACGTAGTAACCACAGAAACAAGA 567 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CAAAGGCTCTTATATAGTCACTAGAACACGTAGTAACCACAGAAACAAGA 100 Wildtype 568 TTTCTGTGTCTAACATATCTCATCACTTCACATTCGGTATCAAACCTCTT 617 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TTTCTGTGTCTAACATATCTCATCACTTCACATTCGGTATCAAACCTCTT 150 Wildtype 618 GCATACTTGCTCATTTTCCAAATCCAGAACCTTAATGGCCACTGCAGTTC 667 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GCATACTTGCTCATTTTCCAAATCCAGAACCTTAATGGCCACTGCAGTTC 200 Wildtype 668 CACCAAAC-AATGCACCTTTGTACACAGAGCCAGAGCTTCCCTCACCAAT 716 \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 CACCAAACAAATGCACCTTTGTACACAGAGCCAGAGCTTCCCTCACCAAT 250 gRNA AAC-AATGCACCTTTGTACAC Wildtype 717 TAAATTGGATTCATCAAAATTATTTGTTGCTCGTTGAATCTCGCTGTAGG 766 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TAAATTGGATTCATCAAAATTATTTGTTGCTCGTTGAATCTCGCTGTAGG 300 Wildtype 767 GGCAGCGATTTAAAAAAAAATTAAAAAATATTAAATCGTTGT 808 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 GGCAGCGATTTAAAAAAAAATTAAAAAATATTAAATCGTTGT 342	Morphology : "Stunted, bleached plants"

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0137-11-8 (T1 )

CRT0137

CTG0137-21

CGR0137-21

gene : Solyc04g014650 (LRRXII-RLK)

-2 bp/+1 bp

Morphology : "Stunted, bleached plants"

CRT0137-11-8 (T1 )

CRT0137

CTG0137-21

CGR0137-21

gene : Solyc04g014890 (LRRXII-RLK)

+1 bp/+1 bp

Is tDNA present: No

Mutation sequence:

ACAACGATTTAATATTTTTTAATTTTTTTTTAAATCGCTGCCCCTACAGCGAGATTCAACGAGCAACAAATAATTTTGAT
GAATCCAATTTAATTGGTGAGGGAAGCTCTGGCTCTGTGTACAAAGGTGCATTTGTTTGGTGGAACTGCAGTGGCCATTA
AGGTTCTGGATTTGGAAAATGAGCAAGTATGCAAGAGGTTTGATACCGAATGTGAAGTGATGAGATATGTTAGACACAGA
AATCTTGTTTCTGTGGTTACTACGTGTTCTAGTGACTATATAAGAGCCTTTGTTCTGCATTTTATGCCCAATGGAAGTCT
TGAGAATTGGTTGTACAAAGAG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         468 CTCTTTGTACAACCAATTCTCAAGACTTCCATTGGGCATAAAATGCAGAA    517
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 CTCTTTGTACAACCAATTCTCAAGACTTCCATTGGGCATAAAATGCAGAA     50

Wildtype         518 CAAAGGCTCTTATATAGTCACTAGAACACGTAGTAACCACAGAAACAAGA    567
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 CAAAGGCTCTTATATAGTCACTAGAACACGTAGTAACCACAGAAACAAGA    100

Wildtype         568 TTTCTGTGTCTAACATATCTCATCACTTCACATTCGGTATCAAACCTCTT    617
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 TTTCTGTGTCTAACATATCTCATCACTTCACATTCGGTATCAAACCTCTT    150

Wildtype         618 GCATACTTGCTCATTTTCCAAATCCAGAACCTTAATGGCCACTGCAGTTC    667
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GCATACTTGCTCATTTTCCAAATCCAGAACCTTAATGGCCACTGCAGTTC    200

Wildtype         668 CACCAAAC-AATGCACCTTTGTACACAGAGCCAGAGCTTCCCTCACCAAT    716
                     |||||||| |||||||||||||||||||||||||||||||||||||||||
MutationRev      201 CACCAAACAAATGCACCTTTGTACACAGAGCCAGAGCTTCCCTCACCAAT    250
gRNA                      AAC-AATGCACCTTTGTACAC

Wildtype         717 TAAATTGGATTCATCAAAATTATTTGTTGCTCGTTGAATCTCGCTGTAGG    766
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 TAAATTGGATTCATCAAAATTATTTGTTGCTCGTTGAATCTCGCTGTAGG    300

Wildtype         767 GGCAGCGATTTAAAAAAAAATTAAAAAATATTAAATCGTTGT    808
                     ||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 GGCAGCGATTTAAAAAAAAATTAAAAAATATTAAATCGTTGT    342

Morphology : "Stunted, bleached plants"