Is tDNA present: No

Mutation sequence:

AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTCAACCTTACCTGAAACCCTGTGGAGCCTTCA
AGATCTCTTGGAACTCAATGCATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTAAAGGCTGCGT
CAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATATTCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTT
TCCATGGCACACAATAAATTAGAAGGGCCTATTCCGAATTCGGGAAAATGGTGGGGTTGGAATTCTTGGATTTTTCTTAC
AACAATCTTACTAGTGAAATTCCAAAGTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACAAATT
AAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGTCAATCTTTCATTTCCAATTCTGCACTATGTGGTG
CTCCTCGGTTTAATGTGTCGCCATGCCTCATC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1911 AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTC   1960
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTC     50

Wildtype        1961 AACCTTACCTGAAACCCTGTGGAGCCTTCAAGATCTCTTGGAACTCAATG   2010
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AACCTTACCTGAAACCCTGTGGAGCCTTCAAGATCTCTTGGAACTCAATG    100

Wildtype        2011 CATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTA   2060
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 CATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTA    150

Wildtype        2061 AAGGCTGCGTCAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATAT   2110
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 AAGGCTGCGTCAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATAT    200

Wildtype        2111 TCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTTTCCATGGCAC   2160
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 TCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTTTCCATGGCAC    250

Wildtype        2161 ACAATAAATTAGAAGGGCCTATTCCGAGTTCATTCGGGAAAATGGTGGGG   2210
                     |||||||||||||||||||||||||||    |||||||||||||||||||
Mutation         251 ACAATAAATTAGAAGGGCCTATTCCGA----ATTCGGGAAAATGGTGGGG    296
gRNA                                GGCCTATTCCGAGTTCATTC

Wildtype        2211 TTGGAATTCTTGGATTTTTCTTACAACAATCTTACTAGTGAAATTCCAAA   2260
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         297 TTGGAATTCTTGGATTTTTCTTACAACAATCTTACTAGTGAAATTCCAAA    346

Wildtype        2261 GTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACA   2310
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         347 GTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACA    396

Wildtype        2311 AATTAAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGT   2360
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         397 AATTAAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGT    446

Wildtype        2361 CAATCTTTCATTTCCAATTCTGCACTATGTGGTGCTCCTCGGTTTAATGT   2410
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         447 CAATCTTTCATTTCCAATTCTGCACTATGTGGTGCTCCTCGGTTTAATGT    496

Wildtype        2411 GTCGCCATGCCTCATC   2426
                     ||||||||||||||||
Mutation         497 GTCGCCATGCCTCATC    512