Is tDNA present: No

Mutation sequence:

GGTGACTTTGGATTAGCAACATTTCTCCTCGACACATCAAGTAATTCTTGGAGCCATCAGATTTCTGCAGCACTAAAGGG
TTCTATAGGTTACATCCCAACAGGTACAACGATGTCATTAGTTTACATTTTAATATTCAGTCATATATCATGCTGCAGAA
AATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCAACCATCCAACTTGGAGATGTTTACAGCTTTGGAATAGTGT
TGTTAGAGTTGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAACATTCACAAGTATGTTTCAACA
GCTTTGCCTGAACATGTTATGGAGATTGTAGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATCA
AGCAAGAAGAGTGGAAGAATGTCTACTCTCGGTTTTGGAAATCGGG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2575 GGTGACTTTGGATTAGCAACATTTCTCCTCGACACATCAAGTAATTCTTG   2624
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 GGTGACTTTGGATTAGCAACATTTCTCCTCGACACATCAAGTAATTCTTG     50

Wildtype        2625 GAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAA   2674
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 GAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAA    100

Wildtype        2675 CAGGTACAACGATGTCATTAGTTTACATTTTAATATTCAGTCATATATCA   2724
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 CAGGTACAACGATGTCATTAGTTTACATTTTAATATTCAGTCATATATCA    150

Wildtype        2725 TGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCA   2774
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCA    200
gRNA                                                           GGCGGCCA

Wildtype        2775 ACCATCCACACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGT   2824
                     |||||||| |||||||||||||||||||||||||||||||||||||||||
Mutation         201 ACCATCCA-ACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGT    249
gRNA                 GGCGGCCAACCATCCACACT

Wildtype        2825 TGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAAC   2874
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 TGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAAC    299

Wildtype        2875 ATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGT   2924
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 ATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGT    349

Wildtype        2925 AGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATC   2974
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         350 AGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATC    399

Wildtype        2975 AAGCAAGAAGAGTGGAAGAATGTCTACTCTCGGTTTTGGAAATCGGG   3021
                     |||||||||||||||||||||||||||||||||||||||||||||||
Mutation         400 AAGCAAGAAGAGTGGAAGAATGTCTACTCTCGGTTTTGGAAATCGGG    446